DK2699668T3 - Filamentøse svampe med en ændret viskositetsfænotype - Google Patents
Filamentøse svampe med en ændret viskositetsfænotype Download PDFInfo
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- DK2699668T3 DK2699668T3 DK12717561.0T DK12717561T DK2699668T3 DK 2699668 T3 DK2699668 T3 DK 2699668T3 DK 12717561 T DK12717561 T DK 12717561T DK 2699668 T3 DK2699668 T3 DK 2699668T3
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- tps2
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/36—Adaptation or attenuation of cells
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
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- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
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- Proteomics, Peptides & Aminoacids (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Peptides Or Proteins (AREA)
Claims (18)
- FILAMENTØSE SVAMPE MED EN ÆNDRET VISKOSITETSFÆNOTYPE PATENTKRAV1. Variantstamme af filamentøs svamp, der er afledt af en moderstamme, hvilken variantstamme indbefatter en genetisk ændring, der omfatter en disruption af ips2-genet, der bevirker, at celler af variantstammen producerer en reduceret mængde funktionelt Tps2-protein sammenlignet med celler af moderstammen, hvor cellerne af variantstammen under aerob fermentation i nedsænket kultur producerer en cellebouillon, der (i) kræver en reduceret mængde omrøring for at bevare et forhåndsvalgt opløst oxygenindhold sammenlignet med moderstammens celler, og/eller (ii) bevarer et øget opløst oxygenindhold ved en forhåndsvalgt mængde omrøring, sammenlignet med moderstammens celler, hvor variantstammen endvidere omfatter et gen, der koder for et protein af interesse, til ekspression af proteinet i de filamentøse svampeceller.
- 2. Variantstamme ifølge krav 1, hvor: (a) den genetiske ændring, der omfatter en disruption af ips2-genet, er resultatet af deletering af hele eller en del af ips2-genet; eller (b) den genetiske ændring, der omfatter en disruption af tps2-genet, er resultatet af deletering af en del af genomisk DNA, der omfatter ips2-genet; eller (c) den genetiske ændring, der omfatter en disruption af ips2-genet, er resultatet af mutagenese af ips2-genet.
- 3. Variantstamme ifølge krav 2, hvor disruption af ips2-genet udføres ved anvendelse af stedsspecifik rekombination eller disruption af ips2-genet udføres i kombination med indføring af en valgbar markør ved det genetiske locus for ips2-genet.
- 4. Variantstamme ifølge et hvilket som helst af de foregående krav, hvor variantstammen ikke producerer funktionelt Tps2-protein.
- 5. Variantstamme ifølge et hvilket som helst af de foregående krav, hvor variantstammen ikke producerer Tps2-protein.
- 6. Variantstamme ifølge et hvilket som helst af de foregående krav, der endvidere omfatter en disruption af s/fc3-genet.
- 7. Variantstamme ifølge et hvilket som helst af de foregående krav, hvor variantstammen producerer mere af proteinet af interesse pr. biomassemængdeenhed end moderstammen.
- 8. Variantstamme ifølge et hvilket som helst af de foregående krav, hvor den filamentøse svamp er en Pezizomycotina-ait eller den filamentøse svamp er en Trichoderma spp.
- 9. Variantstamme ifølge krav 8, hvor den filamentøse svamp er Trichoderma reesei.
- 10. Variantstamme ifølge et hvilket som helst af de foregående krav, hvor variantstammen endvidere omfatter en dismption af mindst ét gen, der er udvalgt fra gruppen bestående af sft>3-genet, seW-genet, mpgl-genet, ga,v/-genet og c/z/-genet.
- 11. Fremgangsmåde til at producere en variantstamme af filamentøse svampeceller, hvilken fremgangsmåde omfatter: indføring af en genetisk ændring i en moderstamme af filamentøs svampecelle, hvilken genetisk ændring omfatter disruption af ips2-genet ved anvendelse af genmanipulation til at reducere eller forhindre produktionen af funktionelt Tps2-protein sammenlignet med moderstammens celler, hvorved der produceres en filamentøs variantsvampecelle, der under aerob fermentation i nedsænket kultur producerer en cellebouillon, der (i) kræver en reduceret mængde omrøring for at bevare et forhåndsvalgt opløst oxygenindhold, sammenlignet med moderstammens celler, og/eller (ii) bevarer et øget opløst oxygenindhold ved en forhåndsvalgt mængde omrøring, sammenlignet med moderstammens celler, hvor variantstammen endvidere omfatter et gen, der koder for et protein af interesse, til ekspression af proteinet i de filamentøse svampeceller.
- 12. Fremgangsmåde ifølge krav 11, hvor: (a) den genetiske ændring omfatter deletering af tps2-genet i en filamentøs modersvampecelle ved anvendelse af genmanipulation; eller (b) den genetiske ændring udføres ved anvendelse af stedsspecifik genetisk rekombination.
- 13. Fremgangsmåde ifølge krav 11 eller krav 12, hvor: (a) disruption af tps2-genet udføres i kombination med indføring af en valgbar markør ved det genetiske locus for tps2-genet; eller (b) disruption af tps2-genet udføres i kombination med disruption af sft>3-genet.
- 14. Fremgangsmåde ifølge et hvilket som helst af kravene 11 til 13, hvor variantstammen producerer mere af proteinet af interesse pr. biomassemængdeenhed end moderstammen.
- 15. Fremgangsmåde ifølge et hvilket som helst af kravene 11 til 14, hvor den filamentøse svamp er en Pezizomycotina-ait eller den filamentøse svamp er en Trichoderma spp.
- 16. Fremgangsmåde ifølge krav 15, hvor den filamentøse svamp er Trichoderma reesei.
- 17. Fremgangsmåde ifølge et hvilket som helst af kravene 11 til 16, hvor genet, der koder for proteinet af interesse, er til stede i moderstammen før indføring af den genetiske ændring, der reducerer eller forhindrer produktionen af funktionelt Tps2-protein.
- 18. Fremgangsmåde ifølge et hvilket som helst af kravene 11 til 17, hvor disruption af /p,v2-gcnet udføres i kombination med disruption af mindst ét gen, der er udvalgt fra gruppen bestående af sfb3-genet, sebi-genet, mpgl-genet, gas/-genet og crzl-genet.
Applications Claiming Priority (6)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161478160P | 2011-04-22 | 2011-04-22 | |
| US201161478162P | 2011-04-22 | 2011-04-22 | |
| US201161480629P | 2011-04-29 | 2011-04-29 | |
| US201161480610P | 2011-04-29 | 2011-04-29 | |
| US201161480602P | 2011-04-29 | 2011-04-29 | |
| PCT/US2012/034409 WO2012145598A1 (en) | 2011-04-22 | 2012-04-20 | Filamentous fungi having an altered viscosity phenotype |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK2699668T3 true DK2699668T3 (da) | 2018-02-26 |
Family
ID=46018122
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK12717560.2T DK2699667T3 (da) | 2011-04-22 | 2012-04-20 | Filamentøse fungi med en ændret viscositetsfænotype |
| DK12717561.0T DK2699668T3 (da) | 2011-04-22 | 2012-04-20 | Filamentøse svampe med en ændret viskositetsfænotype |
| DK12717558.6T DK2673290T3 (da) | 2011-04-22 | 2012-04-20 | Filamentøse svampe med en ændret viskositetsfænotype |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK12717560.2T DK2699667T3 (da) | 2011-04-22 | 2012-04-20 | Filamentøse fungi med en ændret viscositetsfænotype |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK12717558.6T DK2673290T3 (da) | 2011-04-22 | 2012-04-20 | Filamentøse svampe med en ændret viskositetsfænotype |
Country Status (10)
| Country | Link |
|---|---|
| US (3) | US9587242B2 (da) |
| EP (3) | EP2699667B1 (da) |
| JP (3) | JP6042867B2 (da) |
| KR (3) | KR101952469B1 (da) |
| CN (3) | CN103502430B (da) |
| AU (4) | AU2012245319A1 (da) |
| BR (3) | BR112013026535A2 (da) |
| CA (3) | CA2833765C (da) |
| DK (3) | DK2699667T3 (da) |
| WO (3) | WO2012145596A1 (da) |
Families Citing this family (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK3000870T3 (da) * | 2011-04-22 | 2018-04-30 | Danisco Us Inc | Filamentøse svampe med ændret viscositetsfænotype |
| EP2699667B1 (en) * | 2011-04-22 | 2016-04-13 | Danisco US Inc. | Filamentous fungi having an altered viscosity phenotype |
| CN104736694B (zh) | 2012-10-22 | 2020-08-11 | 默沙东公司 | Crz1突变型真菌细胞 |
| KR20200026878A (ko) | 2017-06-06 | 2020-03-11 | 지머젠 인코포레이티드 | 균류 균주를 개량하기 위한 htp 게놈 공학 플랫폼 |
| EP3755710A1 (en) * | 2018-04-24 | 2020-12-30 | Danisco US Inc. | Filamentous fungal strains comprising reduced viscosity phenotypes |
| US11028401B2 (en) | 2018-06-06 | 2021-06-08 | Zymergen Inc. | Manipulation of genes involved in signal transduction to control fungal morphology during fermentation and production |
| CA3100909A1 (en) * | 2018-06-08 | 2019-12-12 | Emergy Inc. | Methods for growing fungal mycelium and forming edible products therefrom |
| CN112654704A (zh) * | 2018-07-30 | 2021-04-13 | 丹尼斯科美国公司 | 包含蛋白质生产率提高表型的突变和遗传修饰的丝状真菌菌株及其方法 |
| EP3831929A4 (en) * | 2018-07-30 | 2022-04-27 | Toray Industries, Inc. | MUTATION OF TRICHODERMA THREAD FUNGUS AND METHOD OF PRODUCING PROTEIN |
| WO2020075788A1 (ja) * | 2018-10-11 | 2020-04-16 | 東レ株式会社 | トリコデルマ・リーセイの変異株およびタンパク質の製造方法 |
| US20200354756A1 (en) * | 2019-04-03 | 2020-11-12 | Danisco Us Inc | Disruption of mvb12 in yeast is associated with increased alcohol production and tolerance |
| FR3104169B1 (fr) * | 2019-12-04 | 2022-10-21 | Ifp Energies Now | Souche de champignon ayant une viscosite diminuee |
| US20220396817A1 (en) * | 2020-02-14 | 2022-12-15 | Inbiose N.V. | Production of glycosylated product in host cells |
| EP3926039A1 (en) | 2020-06-17 | 2021-12-22 | AB Enzymes GmbH | Use of a nuclease for reducing the viscosity and/or preventing an increase in viscosity of a fermentation broth |
| US11479779B2 (en) | 2020-07-31 | 2022-10-25 | Zymergen Inc. | Systems and methods for high-throughput automated strain generation for non-sporulating fungi |
| CN111944779B (zh) * | 2020-08-28 | 2022-06-21 | 山东省科学院生物研究所 | 一种海藻糖合成双功能酶编码基因TvTPS/TPP及其应用 |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FI943133A0 (fi) * | 1994-06-29 | 1994-06-29 | Alko Ab Oy | Transgena vaexter |
| US7189538B2 (en) | 1999-03-24 | 2007-03-13 | Genencor International, Inc. | Hyphal growth in fungi |
| CA2378016A1 (en) * | 1999-07-30 | 2001-02-08 | Margreet Heerikhuisen | Use of aspergillus sojae as host for recombinant protein production |
| MXPA01012905A (es) * | 2000-04-13 | 2002-11-22 | Mark Aaron Emalfarb | Clasificacion de alto rendimiento de bibliotecas de adn expresado en hongo filamentos. |
| KR100903780B1 (ko) * | 2000-04-13 | 2009-06-19 | 다이아딕 인터내셔널 (유에스에이), 인크. | 사상균 크리소스포리움 분야에서의 발현 조절 서열 및발현 생성물 |
| US6936449B2 (en) * | 2001-03-14 | 2005-08-30 | Genencor International, Inc. | Regulatable growth of filamentous fungi |
| WO2003093814A1 (en) * | 2002-05-03 | 2003-11-13 | Dsm Ip Assets B.V. | Process for the monitoring of contaminant removal during the purification process of a pharmaceutical product produced by a host cell |
| EP1862539B1 (en) | 2003-05-29 | 2012-01-25 | Danisco US Inc. | Novel trichoderma genes |
| JP4834554B2 (ja) * | 2003-11-06 | 2011-12-14 | ジェネンコー・インターナショナル・インク | 糸状菌におけるプロテアーゼ抑制剤及びその変異体の発現 |
| ATE530570T1 (de) * | 2006-02-28 | 2011-11-15 | Suntory Holdings Ltd | Für trehalose-6-phosphatphosphatase codierendes gen und verwendung davon |
| US10266832B2 (en) | 2010-08-25 | 2019-04-23 | Danisco Us Inc | Filamentous fungi having an altered viscosity phenotype |
| EP2699667B1 (en) * | 2011-04-22 | 2016-04-13 | Danisco US Inc. | Filamentous fungi having an altered viscosity phenotype |
| DK3000870T3 (da) * | 2011-04-22 | 2018-04-30 | Danisco Us Inc | Filamentøse svampe med ændret viscositetsfænotype |
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2012
- 2012-04-20 EP EP12717560.2A patent/EP2699667B1/en active Active
- 2012-04-20 CA CA2833765A patent/CA2833765C/en active Active
- 2012-04-20 DK DK12717560.2T patent/DK2699667T3/da active
- 2012-04-20 AU AU2012245319A patent/AU2012245319A1/en not_active Abandoned
- 2012-04-20 WO PCT/US2012/034405 patent/WO2012145596A1/en active Application Filing
- 2012-04-20 CN CN201280019665.6A patent/CN103502430B/zh active Active
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- 2012-04-20 US US14/113,930 patent/US9587242B2/en active Active
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- 2012-04-20 CN CN201280019432.6A patent/CN103517981B/zh active Active
- 2012-04-20 CA CA2833756A patent/CA2833756C/en active Active
- 2012-04-20 EP EP12717558.6A patent/EP2673290B1/en active Active
- 2012-04-20 CA CA2833539A patent/CA2833539C/en active Active
- 2012-04-20 WO PCT/US2012/034409 patent/WO2012145598A1/en active Application Filing
- 2012-04-20 BR BR112013026535A patent/BR112013026535A2/pt not_active Application Discontinuation
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- 2012-04-20 KR KR1020137030515A patent/KR101952469B1/ko active IP Right Grant
- 2012-04-20 AU AU2012245325A patent/AU2012245325B2/en not_active Ceased
- 2012-04-20 KR KR1020137030475A patent/KR20140033047A/ko not_active Application Discontinuation
- 2012-04-20 US US14/113,799 patent/US9593341B2/en active Active
- 2012-04-20 BR BR112013026559A patent/BR112013026559A2/pt not_active Application Discontinuation
- 2012-04-20 WO PCT/US2012/034399 patent/WO2012145592A1/en active Application Filing
- 2012-04-20 AU AU2012245323A patent/AU2012245323B2/en not_active Ceased
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2017
- 2017-09-28 AU AU2017235968A patent/AU2017235968B2/en not_active Ceased
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| DK2699668T3 (da) | Filamentøse svampe med en ændret viskositetsfænotype | |
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