DK2681334T3 - Påføring af quantum dots til nuklear farvning - Google Patents

Påføring af quantum dots til nuklear farvning Download PDF

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DK2681334T3
DK2681334T3 DK12709822.6T DK12709822T DK2681334T3 DK 2681334 T3 DK2681334 T3 DK 2681334T3 DK 12709822 T DK12709822 T DK 12709822T DK 2681334 T3 DK2681334 T3 DK 2681334T3
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dna
conjugate
nanoparticle
quantum dot
tissue sample
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Chol S Yun
Brian D Kelly
Julia Ashworth-Sharpe
Christopher Bieniarz
Pascal Bamford
Adrian E Murillo
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Ventana Med Syst Inc
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
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Claims (17)

1. Konjugat, omfattende: en nanopartikel; og en DNA-bindende enhed omfattende et DNA-bindende molekyle og en linker, hvor det DNA-bindende molekyle er en lille-rille binder (minor groove binder), en stor-rille binder (major groove binder), en DNA-interkalator, et DNA-alkyleringsmiddel, eller en kombination deraf, og hvor konjugatet har strukturen nanopartikel - linker - DNA-bindende molekyle.
2. Konjugatet ifølge krav 1, hvor nanopartiklen omfatter en quantum dot, en metalnanopartikel, en metaloxidnanopartikel, eller en overgangsmetalkompleksnanopartikel.
3. Konjugatet ifølge krav 1, hvor den DNA-bindende del omfatter et DNA-bindende molekyle valgt fra 4',6-diamidino-2-phenylindol (DAPI), bis-benzimidfarve, psoralen, og naphthalendiimid.
4. Konjugatet ifølge krav 1, hvor linkeren omfatter en alifatisk kæde fra 1 til 30 carbonatomer i længde eller (-OCH2CH2-)n hvor n er fra 2 til 15.
5. Konjugatet ifølge krav 1, hvor linkeren er en multifunktionel linker og en flerhed af DNA-bindende molekyler er bundet til den multifunktionelle linker.
6. Konjugatet ifølge krav 1, hvor den DNA-bindende del er
7. Fremgangsmåde til visualisering afen cellekerne, omfattende at: forbehandle en vævsprøve med en protease til at danne en forbehandlet vævsprøve; inkubere den forbehandlede vævsprøve med et konjugat ifølge et hvilket som helst af kravene 1 til 6 under betingelser der er tilstrækkelige til at tillade konjugatet at trænge ind i en kerne inde i den forbehandlede vævsprøve, hvor konjugatet binder til DNA i kernen; and visualisere nanopartiklen, og derved visualisere kernen.
8. Fremgangsmåden ifølge krav 7, hvor nanopartiklen omfatter en quantum dot og visualisere nanopartiklen der omfatter visualisering af fotostabil fluorescens af quantum dots.
9. Fremgangsmåden ifølge krav 7, hvor konjugatet er inkuberet med vævsprøven ved en koncentration på mindst 20 nM.
10. Fremgangsmåden ifølge krav 7, yderligere omfattende anvendelse af computerbilleddannelsesanalyseteknikker til kvantitativt at måle nukleartræk, eventuelt hvor nukleartrækkene omfatter kromosomal spredning, ploidi, form, størrelse, teksturtræk, kontekstuelle træk, eller kombinationer deraf.
11. Fremgangsmåden ifølge krav 7, hvor vævsprøven et forbehandlet med proteasen i 4-8 minutter og vævsprøven fikseres før forbehandling med proteasen.
12. Fremgangsmåden ifølge krav 7, yderligere omfattende at: tilvejebringe en probe der er i stand til at hybridisere til et mål inde i vævsprøven før inkubering af den forbehandlede vævsprøve med konjugatet; inkubere proben med vævsprøven under betingelser der er tilstrækkelige til at tillade proben at hybridisere til målet inde i vævsprøven; og påvise proben.
13. Fremgangsmåden ifølge krav 12, hvor påvisning af proben omfatter visualisering af en quantum dot associeret med proben, og hvor nanopartiklen af konjugatet omfatter en quantum dot der er i stand til at sende fluorescens ved en forskellig bølgelængde end en quantum doten der er associeret med proben.
14. Fremgangsmåden ifølge krav 7, yderligere omfattende udføre en fluorescens in situ-hybridiseringsprocedure på vævsprøven, eventuelt hvor fluorescens-in situ-hybridiseringsproceduren omfatter et HER2-assay, et TMPRSS2-ERG-assay, et Chrl7-assay, eller en kombination deraf.
15. Kit til visualisering af kernen, omfattende: en proteaseenzymsammensætning omfattende et proteaseenzym og en proteasebuffer, hvor proteasebufferen har en saltkoncentration og pH tilstrækkelig til at tillade proteaseenzymet at udvise proteolytisk aktivitet; konjugat omfattende a) en nanopartikel og b) en DNA-bindende enhed omfattende et DNA-bindende molekyle; og en reaktionsbuffer, hvor reaktionsbufferen har en saltkoncentration og pH der er tilstrækkelig til at tillade konjugatet at trænge ind i en kerne inden i en vævsprøve forbehandlet med proteaseenzymsammensætningen.
16. Kittet ifølge krav 15, hvor nanopartiklen omfatter en quantum dot, en metalnanopartikel, en metaloxidnanopartikel, eller en overgangsmetalkompleksnanopartikel.
17. Kittet ifølge krav 15, hvor det DNA-bindende molekyle er en lille-rille binder (minor groove binder), en stor-rille binder (major groove binder), en DNA-interkalator, et DNA-alkyleringsmiddel, eller en kombination deraf.
DK12709822.6T 2011-02-28 2012-02-27 Påføring af quantum dots til nuklear farvning DK2681334T3 (da)

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US201161464217P 2011-02-28 2011-02-28
PCT/EP2012/053255 WO2012116949A1 (en) 2011-02-28 2012-02-27 Application of quantum dots for nuclear staining

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Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9156986B2 (en) * 2003-04-03 2015-10-13 Enzo Life Sciences, Inc. Multisignal labeling reagents and processes and uses therefor
US9696298B2 (en) 2003-04-03 2017-07-04 Enzo Life Sciences, Inc. Multisignal reagents for labeling analytes
KR20180081561A (ko) 2015-11-06 2018-07-16 벤타나 메디컬 시스템즈, 인코포레이티드 대표 진단법
CN105543395B (zh) * 2016-02-22 2019-03-29 深圳优圣康医学检验实验室 检测乳腺癌her2基因的纳米量子点标记分子探针及其制备方法
US10747999B2 (en) * 2017-10-18 2020-08-18 The Trustees Of Columbia University In The City Of New York Methods and systems for pattern characteristic detection
JP7038209B2 (ja) 2017-11-13 2022-03-17 エフ．ホフマン－ラ ロシュ アーゲー エピタコ電気泳動を使用する試料分析のための装置
CN109030440B (zh) * 2018-07-18 2020-12-04 西北农林科技大学 一种基于三氧化钼量子点检测单宁酸含量的方法
US10908092B2 (en) 2018-10-11 2021-02-02 Baker Hughes, A Ge Company, Llc Process for making cyano functionalized gold nanoparticles
WO2020074742A1 (en) 2018-10-12 2020-04-16 F. Hoffmann-La Roche Ag Detection methods for epitachophoresis workflow automation
CN111333622B (zh) * 2018-12-18 2022-08-09 中国科学院大连化学物理研究所 含活性酯的高亮度、高稳定性荧光染料及其合成和应用
US20220325268A1 (en) 2019-05-14 2022-10-13 Roche Sequencing Solutions, Inc Devices and methods for sample analysis
WO2023127783A1 (ja) * 2021-12-28 2023-07-06 三菱ケミカル株式会社 電子写真感光体、電子写真感光体カートリッジ、画像形成装置、電子写真感光体保護層形成用塗布液、並びに、化合物

Family Cites Families (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5990479A (en) 1997-11-25 1999-11-23 Regents Of The University Of California Organo Luminescent semiconductor nanocrystal probes for biological applications and process for making and using such probes
JP4630459B2 (ja) 1998-09-24 2011-02-09 インディアナ・ユニバーシティ・リサーチ・アンド・テクノロジー・コーポレーション 水溶性発光量子ドットおよびその生体分子コンジュゲート
JP2003522962A (ja) 2000-02-16 2003-07-29 クァンタム・ドット・コーポレイション 半導体ナノクリスタルを使用するマイクロアレイ法
DE60128458T2 (de) 2000-03-20 2008-01-10 Massachusetts Institute Of Technology, Cambridge Anorganische teilchenkonjugate
US6649138B2 (en) 2000-10-13 2003-11-18 Quantum Dot Corporation Surface-modified semiconductive and metallic nanoparticles having enhanced dispersibility in aqueous media
EP1215199A1 (en) 2000-12-08 2002-06-19 Sony International (Europe) GmbH Linker molecules for selective metallisation of nucleic acids and their uses
US20020083888A1 (en) 2000-12-28 2002-07-04 Zehnder Donald A. Flow synthesis of quantum dot nanocrystals
US20020127224A1 (en) 2001-03-02 2002-09-12 James Chen Use of photoluminescent nanoparticles for photodynamic therapy
JP2005511761A (ja) * 2001-07-10 2005-04-28 ノース・キャロライナ・ステイト・ユニヴァーシティ ナノ粒子送達ビヒクル
AUPR647801A0 (en) 2001-07-19 2001-08-09 Cea Technologies Inc. Chromatin segmentation
CA2453450A1 (en) 2001-07-20 2003-11-06 Quantum Dot Corporation Luminescent nanoparticles and methods for their preparation
JP2004065040A (ja) 2002-08-02 2004-03-04 Japan Science & Technology Corp マウスナノス様遺伝子
US7981667B2 (en) 2003-05-07 2011-07-19 Indiana University Research And Technology Corporation Alloyed semiconductor quantum dots and concentration-gradient alloyed quantum dots, series comprising the same and methods related thereto
KR100657891B1 (ko) 2003-07-19 2006-12-14 삼성전자주식회사 반도체 나노결정 및 그 제조방법
JP4111099B2 (ja) 2003-08-21 2008-07-02 ソニー株式会社 生化学分析方法及び装置
WO2007013877A2 (en) 2004-09-02 2007-02-01 The Regents Of The University Of California Signal peptide-semiconductor nanocrystal conjugates
US20060246524A1 (en) 2005-04-28 2006-11-02 Christina Bauer Nanoparticle conjugates
ES2548518T3 (es) 2005-11-23 2015-10-19 Ventana Medical Systems, Inc. Conjugado molecular
US7498177B2 (en) 2006-04-24 2009-03-03 Jesus Martinez De La Fuente Quantum dots and their uses
WO2008028156A2 (en) 2006-09-01 2008-03-06 Ventana Medical Systems, Inc. Method for producing nucleic acid probes
JP5697068B2 (ja) 2006-12-28 2015-04-08 独立行政法人産業技術総合研究所 プルシアンブルー型金属錯体ナノ粒子の製造方法、並びにそれにより得られるプルシアンブルー型金属錯体ナノ粒子、その分散液、その発色制御方法、それを用いた電極及び透過光制御装置
MX2009008470A (es) 2007-02-09 2009-11-26 Univ Northwestern Particulas para detectar objetivos intracelulares.
US8790877B2 (en) 2007-07-12 2014-07-29 The United States Of America As Represented By The Secretary Of The Air Force Using DNA aptamers and quantum dots for the detection of proteins or other targets
KR100951719B1 (ko) 2007-10-02 2010-04-07 재단법인서울대학교산학협력재단 세포투과성 펩타이드와 형광표지 자성나노입자의 복합체 및그 용도
WO2011011782A1 (en) 2009-07-24 2011-01-27 Duke University Functionalized metal-coated energy converting nanoparticles, methods for production thereof and methods for use

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US20150267262A1 (en) 2015-09-24
AU2012222506B2 (en) 2015-08-20
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ES2588514T3 (es) 2016-11-03
CA2824355C (en) 2020-03-31
US9448231B2 (en) 2016-09-20
AU2012222506A1 (en) 2013-07-25
CA2824355A1 (en) 2012-09-07
JP5943943B2 (ja) 2016-07-05
EP2681334A1 (en) 2014-01-08
US20120219948A1 (en) 2012-08-30
WO2012116949A1 (en) 2012-09-07

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