DK2670853T3 - Fremgangsmåde til enzymatisk raffinering af forbehandlet celluloseholdigt materiale til forsukring - Google Patents

Fremgangsmåde til enzymatisk raffinering af forbehandlet celluloseholdigt materiale til forsukring Download PDF

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Publication number
DK2670853T3
DK2670853T3 DK12735971.9T DK12735971T DK2670853T3 DK 2670853 T3 DK2670853 T3 DK 2670853T3 DK 12735971 T DK12735971 T DK 12735971T DK 2670853 T3 DK2670853 T3 DK 2670853T3
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gly
ala
ser
thr
seq
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DK12735971.9T
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Armindo Ribeiro Gaspar
Donald Higgins
Ye Chen
Hui Xu
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Novozymes North America Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08H—DERIVATIVES OF NATURAL MACROMOLECULAR COMPOUNDS
    • C08H8/00—Macromolecular compounds derived from lignocellulosic materials
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
    • C—CHEMISTRY; METALLURGY
    • C13—SUGAR INDUSTRY
    • C13K—SACCHARIDES OBTAINED FROM NATURAL SOURCES OR BY HYDROLYSIS OF NATURALLY OCCURRING DISACCHARIDES, OLIGOSACCHARIDES OR POLYSACCHARIDES
    • C13K1/00—Glucose; Glucose-containing syrups
    • C13K1/02—Glucose; Glucose-containing syrups obtained by saccharification of cellulosic materials
    • D—TEXTILES; PAPER
    • D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
    • D21C—PRODUCTION OF CELLULOSE BY REMOVING NON-CELLULOSE SUBSTANCES FROM CELLULOSE-CONTAINING MATERIALS; REGENERATION OF PULPING LIQUORS; APPARATUS THEREFOR
    • D21C5/00—Other processes for obtaining cellulose, e.g. cooking cotton linters ; Processes characterised by the choice of cellulose-containing starting materials
    • D21C5/005—Treatment of cellulose-containing material with microorganisms or enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P2201/00—Pretreatment of cellulosic or lignocellulosic material for subsequent enzymatic treatment or hydrolysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P2203/00—Fermentation products obtained from optionally pretreated or hydrolyzed cellulosic or lignocellulosic material as the carbon source
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/01—Carboxylic ester hydrolases (3.1.1)
    • C12Y301/01073—Feruloyl esterase (3.1.1.73)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Emergency Medicine (AREA)
  • Materials Engineering (AREA)
  • Medicinal Chemistry (AREA)
  • Polymers & Plastics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Claims (10)

1. Fremgangsmåde til enzymatisk raffinering af et forbehandlet celluloseholdigt materiale, hvilken fremgangsmåde omfatter: - separation af en væske fra det forbehandlede celluloseholdige materiale, - etablering af kontakt mellem væsken og feruloylesterase og - recirkulering af væsken, således at den bringes i kontakt med forbehandlet celluloseholdigt materiale eller med et forbehandlet celluloseholdigt materiale efter etablering af kontakt mellem dette og feruloylesterase for at danne et raffineret forbehandlet celluloseholdigt materiale.
2. Fremgangsmåde ifølge krav 1, hvor feruloylesterasen omfatter en aminosyresekvens, som udviser mindst 70%, 80%, 90%, 95%, 96%, 97%, 98%, 99% sekvensidentitet med det modne polypeptid ifølge SEQ ID NO: 133, SEQ ID NO: 134, SEQ ID NO: 135, SEQ ID NO: 136 eller SEQ ID NO: 137, eller fragmenter deraf med feruloylesteraseaktivitet.
3. Fremgangsmåde ifølge krav 1, hvor feruloylesterasen omfatter eller består af SEQ ID NO: 133, SEQ ID NO: 134, SEQ ID NO: 135, SEQ ID NO: 136 eller SEQ ID NO: 137 eller det modne polypeptid deraf.
4. Fremgangsmåde ifølge et hvilket som helst af kravene 1-3, som endvidere omfatter efterbehandling af det raffinerede forbehandlede celluloseholdige materiale med en enzymatisk forbehandling, kemisk forbehandling, mekanisk forbehandling og/eller en fysisk forbehandling.
5. Fremgangsmåde ifølge et hvilket som helst af kravene 1-4, som endvidere omfatter indvinding af det raffinerede forbehandlede celluloseholdige materiale.
6. Fremgangsmåde ifølge et hvilket som helst af kravene 1-5, hvor etableringen af kontakt med feruloylesterasen udføres med 0,0005 til 5 mg feruloylesterase pr. g forbehandlet celluloseholdigt materiale.
7. Fremgangsmåde ifølge et hvilket som helst af kravene 1-6, hvor etableringen af kontakt med feruloylesterase udføres med et totalt indhold af faste stoffer (total solids - TS) på 1 til 50%.
8. Fremgangsmåde ifølge et hvilket som helst af kravene 1-7, hvor etableringen af kontakt med esterase udføres ved en pH på 2 til 9.
9. Fremgangsmåde ifølge et hvilket som helst af kravene 1-8, hvor etableringen af kontakt udføres ved en temperatur i intervallet fra 20 til 70°C.
10. Fremgangsmåde ifølge et hvilket som helst af kravene 1-9, hvor etableringen af kontakt med feruloylesterase udføres i et tidsrum på fra 5 minutter til 35 timer.
DK12735971.9T 2011-01-31 2012-01-31 Fremgangsmåde til enzymatisk raffinering af forbehandlet celluloseholdigt materiale til forsukring DK2670853T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161438242P 2011-01-31 2011-01-31
PCT/US2012/023217 WO2012134626A2 (en) 2011-01-31 2012-01-31 Processes for enzymatic refining of pretreated cellulosic material for saccharification

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Publication Number Publication Date
DK2670853T3 true DK2670853T3 (da) 2017-08-28

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Country Link
US (1) US9434972B2 (da)
EP (1) EP2670853B1 (da)
CN (1) CN103459605B (da)
BR (1) BR112013019324B1 (da)
DK (1) DK2670853T3 (da)
WO (1) WO2012134626A2 (da)

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US20130337509A1 (en) 2013-12-19
CN103459605A (zh) 2013-12-18
BR112013019324A2 (pt) 2017-03-21
WO2012134626A3 (en) 2013-03-21
BR112013019324B1 (pt) 2021-06-08
EP2670853B1 (en) 2017-05-24
US9434972B2 (en) 2016-09-06
EP2670853A2 (en) 2013-12-11
CN103459605B (zh) 2016-08-24

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