DK2601288T3 - Simplified base media for human pluripotent cell culture - Google Patents
Simplified base media for human pluripotent cell culture Download PDFInfo
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- DK2601288T3 DK2601288T3 DK11751704.5T DK11751704T DK2601288T3 DK 2601288 T3 DK2601288 T3 DK 2601288T3 DK 11751704 T DK11751704 T DK 11751704T DK 2601288 T3 DK2601288 T3 DK 2601288T3
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- cells
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- human
- pluripotent stem
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Families Citing this family (59)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8017395B2 (en) | 2004-12-17 | 2011-09-13 | Lifescan, Inc. | Seeding cells on porous supports |
| US9080145B2 (en) | 2007-07-01 | 2015-07-14 | Lifescan Corporation | Single pluripotent stem cell culture |
| CA2695225C (en) | 2007-07-31 | 2021-06-01 | Lifescan, Inc. | Differentiation of human embryonic stem cells to pancreatic endocrine |
| CA2954431C (en) | 2007-11-27 | 2021-08-24 | Lifescan, Inc. | Differentiation of human embryonic stem cells to pancreatic cells |
| CN102046779A (zh) | 2008-02-21 | 2011-05-04 | 森托科尔奥索生物科技公司 | 用于细胞粘附、培养和分离的方法、表面改性培养板和组合物 |
| EP2942392B1 (en) | 2008-06-30 | 2018-10-03 | Janssen Biotech, Inc. | Differentiation of pluripotent stem cells |
| ES2727950T3 (es) | 2008-10-31 | 2019-10-21 | Janssen Biotech Inc | Diferenciación de células madre embrionarias humanas en linaje endocrino pancreático |
| MX2011004565A (es) | 2008-10-31 | 2011-07-28 | Centocor Ortho Biotech Inc | Diferenciacion de celulas madre embrionarias humanas al linaje endocrino pancreatico. |
| JP5719305B2 (ja) | 2008-11-20 | 2015-05-13 | ヤンセン バイオテツク，インコーポレーテツド | 平面支持体上での細胞付着及び培養のための方法及び組成物 |
| KR101774546B1 (ko) | 2008-11-20 | 2017-09-04 | 얀센 바이오테크 인코포레이티드 | 마이크로-캐리어 상의 만능 줄기 세포 배양 |
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| SG10201501503VA (en) | 2010-03-01 | 2015-04-29 | Janssen Biotech Inc | Methods for purifying cells derived from pluripotent stem cells |
| EP3498825A1 (en) | 2010-05-12 | 2019-06-19 | Janssen Biotech, Inc. | Differentiation of human embryonic stem cells |
| US9279107B2 (en) | 2010-08-05 | 2016-03-08 | Wisconsin Alumni Research Foundation | Simplified basic media for human pluripotent cell culture |
| ES2585028T3 (es) | 2010-08-31 | 2016-10-03 | Janssen Biotech, Inc. | Diferenciación de células madre pluripotentes |
| EP3211070A1 (en) | 2010-08-31 | 2017-08-30 | Janssen Biotech, Inc. | Differentiation of human embryonic stem cells |
| CA2809300A1 (en) | 2010-08-31 | 2012-03-08 | Janssen Biotech, Inc. | Differentiation of human embryonic stem cells |
| US8497124B2 (en) | 2011-12-05 | 2013-07-30 | Factor Bioscience Inc. | Methods and products for reprogramming cells to a less differentiated state |
| CA2858148C (en) | 2011-12-05 | 2023-03-14 | Factor Bioscience Inc. | Methods and products for transfecting cells |
| WO2013090919A1 (en) | 2011-12-16 | 2013-06-20 | Wisconsin Alumni Research Foundation | Fgf-2 having enhanced stability |
| MX2014007744A (es) | 2011-12-22 | 2015-01-12 | Janssen Biotech Inc | Diferenciacion de celulas madre embrionicas humanas en celulas individuales positivas de insulina hormonal. |
| MX354775B (es) | 2012-03-07 | 2018-03-20 | Janssen Biotech Inc | Medios definidos para la expansion y el mantenimiento de celulas madre pluripotentes. |
| DK3450542T3 (da) | 2012-06-08 | 2021-11-01 | Janssen Biotech Inc | Differentiering af humane embryonale stamceller til endokrine pancreatiske celler |
| CA2890110C (en) | 2012-11-01 | 2023-05-02 | Factor Bioscience Inc. | Methods and products for expressing proteins in cells |
| ES2942484T3 (es) | 2012-12-31 | 2023-06-01 | Janssen Biotech Inc | Diferenciación de células madre embrionarias humanas en células endocrinas pancreáticas usando reguladores de HB9 |
| US10344264B2 (en) | 2012-12-31 | 2019-07-09 | Janssen Biotech, Inc. | Culturing of human embryonic stem cells at the air-liquid interface for differentiation into pancreatic endocrine cells |
| RU2687379C2 (ru) | 2012-12-31 | 2019-05-13 | Янссен Байотек, Инк. | Суспендирование и кластеризация плюрипотентных клеток человека с целью их дифференцировки в панкреатические эндокринные клетки |
| US10370644B2 (en) | 2012-12-31 | 2019-08-06 | Janssen Biotech, Inc. | Method for making human pluripotent suspension cultures and cells derived therefrom |
| KR102211266B1 (ko) | 2013-01-31 | 2021-02-04 | 아지노모토 가부시키가이샤 | 다능성 줄기 세포의 안정한 미분화 유지 증식을 실시하기 위한 배양 방법 |
| WO2014208295A1 (ja) | 2013-06-28 | 2014-12-31 | 株式会社カネカ | 多能性幹細胞の増殖促進因子のスクリーニング法 |
| WO2015006762A1 (en) * | 2013-07-12 | 2015-01-15 | The Board Of Trustees Of The University Of Arkansas | Methods and compositions for expanding long-term hematopoietic stem cell populations |
| KR101655383B1 (ko) * | 2013-07-27 | 2016-09-08 | 고려대학교 산학협력단 | 소분자 화합물을 포함하는 만능성 줄기세포의 염색체 안정성 유지용 조성물 |
| US20160230143A1 (en) | 2013-09-19 | 2016-08-11 | The U.S.A., As Represented By The Secretary, Department Of Health & Human Services | Chemically defined culture medium for stem cell maintenance and differentiation |
| RU2714404C2 (ru) | 2014-01-31 | 2020-02-14 | Фэктор Байосайенс Инк. | Способы и продукты для получения и доставки нуклеиновых кислот |
| JP6422221B2 (ja) * | 2014-03-04 | 2018-11-14 | 旭化成株式会社 | 多能性幹細胞からなる細胞塊製造方法 |
| JP6502323B2 (ja) | 2014-03-26 | 2019-04-17 | 国立大学法人京都大学 | 多能性幹細胞培養用培地 |
| JP6588969B2 (ja) | 2014-05-16 | 2019-10-09 | ヤンセン バイオテツク，インコーポレーテツド | 膵内分泌細胞内のｍａｆａ発現を強化するための小分子の使用 |
| EP3177302A4 (en) * | 2014-08-07 | 2018-04-11 | Duke University | Compositions and methods for the reprogramming of cells into cardiomyocytes |
| CN106574248B (zh) | 2014-08-19 | 2020-11-24 | 富士胶片细胞动力有限公司 | 由源自多能干细胞的细胞形成的神经网络 |
| JP6800859B2 (ja) | 2015-01-26 | 2020-12-16 | フェイト セラピューティクス，インコーポレイテッド | 造血細胞分化を誘導するための方法および組成物 |
| EP3543339A1 (en) | 2015-02-13 | 2019-09-25 | Factor Bioscience Inc. | Nucleic acid products and methods of administration thereof |
| KR20250033315A (ko) | 2015-11-04 | 2025-03-07 | 페이트 세러퓨틱스, 인코포레이티드 | 조혈 세포 분화를 유도하기 위한 방법 및 조성물 |
| EP3371314B1 (en) | 2015-11-04 | 2023-07-05 | Fate Therapeutics, Inc. | Genomic engineering of pluripotent cells |
| MA45479A (fr) | 2016-04-14 | 2019-02-20 | Janssen Biotech Inc | Différenciation de cellules souches pluripotentes en cellules de l'endoderme de l'intestin moyen |
| CN116115629A (zh) | 2016-08-17 | 2023-05-16 | 菲克特生物科学股份有限公司 | 核酸产品及其施用方法 |
| KR101953573B1 (ko) * | 2017-03-31 | 2019-03-05 | 한양대학교 에리카산학협력단 | 난황줄기세포 배양을 위한 우태아혈청 조성물 및 이의 제조방법 |
| KR102668891B1 (ko) | 2017-04-18 | 2024-05-29 | 후지필름 셀룰러 다이내믹스, 인코포레이티드 | 항원-특이적 면역 이펙터 세포 |
| US11252954B2 (en) | 2017-09-08 | 2022-02-22 | Nissan Chemical Corporation | Method for preserving a cell material in an unfrozen state |
| KR102121602B1 (ko) * | 2018-06-27 | 2020-06-10 | 주식회사 티아라줄기세포연구소 | 지방줄기세포배양용 배지 조성물 제조방법, 지방줄기세포배양용 배지 조성물 제조방법과 줄기세포 유효성분 3저 추출법을 이용한 줄기세포파쇄추출물(쉘드줄기세포) 제조방법, 이를 이용한 항관절염 치료용 조성물, 이를 이용한 염증억제 효과를 갖는 조성물 및 세포재생 효과를 갖는 조성물 |
| WO2020200071A1 (en) * | 2019-04-05 | 2020-10-08 | The University Of Hong Kong | Culture medium for mammalian expanded potential stem cells, composition, and methods thereof |
| CN119569589A (zh) | 2019-07-03 | 2025-03-07 | 菲克特生物科学股份有限公司 | 阳离子脂质及其用途 |
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| AU2020351221A1 (en) * | 2019-09-19 | 2022-05-05 | Northwestern University | Cost effective culture media and protocol for human induced pluripotent stem cells |
| CN110564673A (zh) * | 2019-09-26 | 2019-12-13 | 广东工业大学 | 一种干细胞培养液及其应用 |
| CN110564678B (zh) * | 2019-10-12 | 2023-04-07 | 广东唯泰生物科技有限公司 | 脐带华通氏胶间充质干细胞成骨定向分化的方法 |
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| GB2432846B (en) | 2004-09-08 | 2009-12-30 | Wisconsin Alumni Res Found | Medium and culture of embryonic stem cells |
| EP3354723B1 (en) * | 2005-08-29 | 2023-12-13 | Technion Research & Development Foundation Ltd. | Media for culturing stem cells |
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| WO2009135206A1 (en) | 2008-05-02 | 2009-11-05 | Stem Cell Products, Inc. | Method for production of mast cells from stem cells |
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| BR112013002811A8 (pt) | 2020-01-28 |
| EP2601288A2 (en) | 2013-06-12 |
| AU2011285531A1 (en) | 2013-02-21 |
| KR101829488B1 (ko) | 2018-02-14 |
| EP2601288B1 (en) | 2016-04-06 |
| WO2012019122A3 (en) | 2012-05-03 |
| KR20130099927A (ko) | 2013-09-06 |
| BR112013002811A2 (pt) | 2016-08-02 |
| US9279103B2 (en) | 2016-03-08 |
| US20120202291A1 (en) | 2012-08-09 |
| WO2012019122A2 (en) | 2012-02-09 |
| JP6043999B2 (ja) | 2016-12-14 |
| CN108531444A (zh) | 2018-09-14 |
| CA2807418A1 (en) | 2012-02-09 |
| BR122020000909B1 (pt) | 2021-02-23 |
| CA2807418C (en) | 2017-04-04 |
| SG187699A1 (en) | 2013-03-28 |
| JP2017018137A (ja) | 2017-01-26 |
| IL224431A (en) | 2017-10-31 |
| JP2013532492A (ja) | 2013-08-19 |
| CN103180434A (zh) | 2013-06-26 |
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