DK2505220T3 - Fremgangsmåde til formering af humane hepatocytafledte celler - Google Patents

Fremgangsmåde til formering af humane hepatocytafledte celler Download PDF

Info

Publication number
DK2505220T3
DK2505220T3 DK12004762.6T DK12004762T DK2505220T3 DK 2505220 T3 DK2505220 T3 DK 2505220T3 DK 12004762 T DK12004762 T DK 12004762T DK 2505220 T3 DK2505220 T3 DK 2505220T3
Authority
DK
Denmark
Prior art keywords
cells
alginate
liver
matrix
cell
Prior art date
Application number
DK12004762.6T
Other languages
English (en)
Inventor
Clare Selden
Humphrey Hodgson
Sam Coward
Original Assignee
Univ London
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ London filed Critical Univ London
Application granted granted Critical
Publication of DK2505220T3 publication Critical patent/DK2505220T3/da

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A01N1/0236—Mechanical aspects
    • A01N1/0242—Apparatuses, i.e. devices used in the process of preservation of living parts, such as pumps, refrigeration devices or any other devices featuring moving parts and/or temperature controlling components
    • A01N1/0247—Apparatuses, i.e. devices used in the process of preservation of living parts, such as pumps, refrigeration devices or any other devices featuring moving parts and/or temperature controlling components for perfusion, i.e. for circulating fluid through organs, blood vessels or other living parts
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A01N1/0236—Mechanical aspects
    • A01N1/0242—Apparatuses, i.e. devices used in the process of preservation of living parts, such as pumps, refrigeration devices or any other devices featuring moving parts and/or temperature controlling components
    • A01N1/0252—Temperature controlling refrigerating apparatus, i.e. devices used to actively control the temperature of a designated internal volume, e.g. refrigerators, freeze-drying apparatus or liquid nitrogen baths
    • A01N1/0257—Stationary or portable vessels generating cryogenic temperatures
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
    • A61F2/00—Filters implantable into blood vessels; Prostheses, i.e. artificial substitutes or replacements for parts of the body; Appliances for connecting them with the body; Devices providing patency to, or preventing collapsing of, tubular structures of the body, e.g. stents
    • A61F2/02—Prostheses implantable into the body
    • A61F2/022—Artificial gland structures using bioreactors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
    • A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
    • A61M1/34—Filtering material out of the blood by passing it through a membrane, i.e. hemofiltration or diafiltration
    • A61M1/3472—Filtering material out of the blood by passing it through a membrane, i.e. hemofiltration or diafiltration with treatment of the filtrate
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
    • A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
    • A61M1/34—Filtering material out of the blood by passing it through a membrane, i.e. hemofiltration or diafiltration
    • A61M1/3472—Filtering material out of the blood by passing it through a membrane, i.e. hemofiltration or diafiltration with treatment of the filtrate
    • A61M1/3486—Biological, chemical treatment, e.g. chemical precipitation; treatment by absorbents
    • A61M1/3489—Biological, chemical treatment, e.g. chemical precipitation; treatment by absorbents by biological cells, e.g. bioreactor
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
    • A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
    • A61M1/34—Filtering material out of the blood by passing it through a membrane, i.e. hemofiltration or diafiltration
    • A61M1/3496—Plasmapheresis; Leucopheresis; Lymphopheresis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
    • A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
    • A61M1/36—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits
    • A61M1/3687—Chemical treatment
    • A61M1/3689—Chemical treatment by biological cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/08—Bioreactors or fermenters specially adapted for specific uses for producing artificial tissue or for ex-vivo cultivation of tissue
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/06—Tubular
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/16—Particles; Beads; Granular material; Encapsulation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
    • C12M41/24—Heat exchange systems, e.g. heat jackets or outer envelopes inside the vessel
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0012—Cell encapsulation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0062—General methods for three-dimensional culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • C12N5/0075—General culture methods using substrates using microcarriers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/067—Hepatocytes
    • C12N5/0671—Three-dimensional culture, tissue culture or organ culture; Encapsulated cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2511/00—Cells for large scale production
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/10—Mineral substrates
    • C12N2533/12—Glass
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/70—Polysaccharides
    • C12N2533/74—Alginate

Claims (13)

  1. Fremgangsmåde til formering af humane hepatocytafledte celler Patentkrav
    1. Skalerbar fremgangsmåde til formering af celler fra en human hepatocytcellelinje omfattende: a) Podning af celler fra en human hepatocytcellelinje ind i et alginatmatrixdannende middel og dannelse af alginatkugler omfattende matrixindkapslede celler og b) Prækultur af de matrixindkapslede celler i et dyrkningsmedium i et kammer med et fluidiseret leje, så de danner sammenhængende sfæroider i et celleformeringsstadium, hvori de matrixindkapslede celler holdes i kontinuerlig suspension i dyrkningsmediet i 8 til 12 dage og dyrkes til ydeevnekompetence, således at de opretholder en næsten-kubisk cellearkitektur, har en tæt celle til celle- og celle til matrix-organisation og afsondrer ekstracellulære matrixproteiner og et repertoire af leverspecifikke afsondrede proteiner.
  2. 2. Skalerbar fremgangsmåde ifølge krav 1, hvori den humane hepatocytcellelinje omfatter Hep G2-celler.
  3. 3. Skalerbar fremgangsmåde ifølge krav 1, hvori alginatkugleme polymeriseres.
  4. 4. Skalerbar fremgangsmåde ifølge krav 3, hvori alginatkugleme polymeriseres i 10- 60 minutter i calciumchloridopløsning indeholdende et eller flere overfladeaktive midler.
  5. 5. Skalerbar fremgangsmåde ifølge krav 1, hvori alginatkugleme omfatter en ikke-coatet alginat.
  6. 6. Skalerbar fremgangsmåde ifølge et hvilket som helst af kravene 3 til 5, hvori alginatkugleme har en middeldiameter på mellem 300-1200 pm.
  7. 7. Skalerbar fremgangsmåde ifølge et hvilket som helst af de foregående krav, hvori de indkapslede celler endvidere omfatter et densitetskontrollerende middel.
  8. 8. Skalerbar fremgangsmåde ifølge krav 7, hvori det densitetskontrollerende middel er glaskugler.
  9. 9. Skalerbar fremgangsmåde ifølge krav 8, hvori glaskugleme har en middeldiameter på mellem 10-50 pm.
  10. 10. Skalerbar fremgangsmåde ifølge et hvilket som helst af de foregående krav, hvori omkring lxlO9 humane hepatocytceller anvendes til at pode fra 500-666 ml alginatkugler.
  11. 11. Skalerbar fremgangsmåde ifølge et hvilket som helst af kravene 1 til 10, hvori de indkapslede celler fremstilles ved en fremgangsmåde omfattende: i. Fremstilling af det alginatmatrixdannende middel; ii. Podning af det alginatmatrixdannende middel med en flerhed af celler fra en human hepatocytcellelinje; iii. Fortætning af det alginatmatrixdannende middel med en densitetsmodifikator, således at kugler af de matrixindkapsiede celler vil opnå kontinuerlig suspension, når de perfunderes i dyrkningsmediet eller i humant plasma; iv. Passage af de humane hepatocytafledte celler gennem en formet dyse, så der dannes en strøm; v. Udskæring af strømmen i kugler; og vi. Polymerisering af kuglerne.
  12. 12. Fremgangsmåde ifølge krav 11 omfattende 0,5xl06 til 3,0xl06 celler pr. ml alginat.
  13. 13. Fremgangsmåde ifølge krav 11, hvori den formede dyse er cirkulær i tværsnit og har en diameter på fra 250-450 pm, og strømmen udskæres med ståltråds skiver ved en hastighed på fra 3000 til 4000 rpm.
DK12004762.6T 2007-07-06 2008-06-25 Fremgangsmåde til formering af humane hepatocytafledte celler DK2505220T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0713595A GB2450761B (en) 2007-07-06 2007-07-06 Multi-functional chamber for housing a biological component
EP08762515.8A EP2170429B1 (en) 2007-07-06 2008-06-25 Multi-functional chamber for housing a biological component

Publications (1)

Publication Number Publication Date
DK2505220T3 true DK2505220T3 (da) 2018-05-07

Family

ID=38461507

Family Applications (1)

Application Number Title Priority Date Filing Date
DK12004762.6T DK2505220T3 (da) 2007-07-06 2008-06-25 Fremgangsmåde til formering af humane hepatocytafledte celler

Country Status (7)

Country Link
US (2) US9402944B2 (da)
EP (2) EP2505220B1 (da)
DK (1) DK2505220T3 (da)
ES (2) ES2666888T3 (da)
GB (2) GB2467645B (da)
NO (1) NO2505220T3 (da)
WO (1) WO2009007678A2 (da)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2467645B (en) * 2007-07-06 2011-12-21 Univ London A method of proliferating human hepatocyte cells
GB2466481B (en) * 2008-12-17 2013-05-29 Ucl Business Plc Improvements in or relating to growing cells
WO2010105204A2 (en) * 2009-03-13 2010-09-16 Mayo Foundation For Medical Education And Research Bioartificial liver
US9534992B2 (en) * 2011-05-18 2017-01-03 Biocision, Llc Ventilation assisted passive cell freezing device
CN103981147B (zh) 2013-02-08 2017-11-10 中国科学院上海生命科学研究院 一种新的制备肝实质细胞的方法
EP2871233A1 (de) * 2013-11-12 2015-05-13 Brandenburgische Technische Universität Cottbus-Senftenberg Verfahren zur Herstellung von biogenen Stoffen
CN104353142B (zh) * 2014-09-30 2017-01-11 南京比瑞生物科技有限公司 一种生物人工肝反应器
DE102016001407A1 (de) * 2016-02-09 2017-08-24 Fresenius Medical Care Deutschland Gmbh Blutbehandlung mit Inaktivierung von freien Nukleinsäuren
CN106139285B (zh) * 2016-07-29 2018-07-06 武汉仝干医疗科技股份有限公司 四合一毛绒线式生物人工肝在线监测与恒温加热一体系统
CN109689853B (zh) 2016-08-27 2022-08-23 三维生物科技有限公司 生物反应器
CN106560419B (zh) * 2016-11-14 2018-10-19 上海原能细胞医学技术有限公司 管阵式液氮罐
NZ753873A (en) 2016-12-05 2023-01-27 Children’S Hospital Medical Center Colonic organoids and methods of making and using same
WO2019053243A1 (en) 2017-09-18 2019-03-21 Santersus Sa METHOD AND DEVICE FOR PURIFYING BLOOD OF FREE CIRCULATING DNA
KR20200138774A (ko) * 2018-05-20 2020-12-10 아베야테크, 엘엘씨 극저온 저장 유닛
KR20210065970A (ko) * 2018-09-27 2021-06-04 칠드런즈 호스피탈 메디칼 센터 간 오가노이드를 포함하는 간 지원 시스템 및 이의 제조 및 사용 방법
CN110205245B (zh) * 2019-06-14 2022-07-08 科先医疗科技(苏州)有限公司 一种用于生物型人工肝支持系统的细胞反应器
CN110317731A (zh) * 2019-08-07 2019-10-11 上海交通大学医学院附属第九人民医院 灌注式生物反应器
JP2023516484A (ja) 2020-03-11 2023-04-19 ビット バイオ リミテッド 肝細胞作製方法
WO2021181110A1 (en) 2020-03-11 2021-09-16 Bit Bio Limited Method of generating hepatic cells
CN111249552B (zh) * 2020-03-16 2020-11-17 南京鼓楼医院 基于人iPSCs诱导类肝细胞及多层多孔生物反应器的生物人工肝
CA3202658A1 (en) * 2020-12-22 2022-06-30 Michal Wszola A system for storing or cultivating of an organ or tissue model and uses thereof
CN113652349B (zh) * 2021-08-24 2023-07-07 威世药业（如皋）有限公司 一种立再适体内分析方法开发和初步药代动力学实验设备

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IT1008963B (it) * 1974-03-01 1976-11-30 Co Tecnica Ind Petro Li Spa Perfezionamento nei fermentatori a trascinamento d aria
DE2940446C2 (de) * 1979-10-05 1982-07-08 B. Braun Melsungen Ag, 3508 Melsungen Züchtung von tierischen Zellen in Suspensions- und Monolayerkulturen in Fermentationsgefäßen
US4311589A (en) * 1979-11-06 1982-01-19 Biomedics, Inc. Toroidal flow blood reactor
JPS60159596A (ja) * 1984-01-30 1985-08-21 Agency Of Ind Science & Technol 生物汚損防止方法
US5270192A (en) * 1991-02-07 1993-12-14 Monsanto Company Biological artificial liver
JP3339685B2 (ja) 1992-02-07 2002-10-28 モンサント カンパニー 生物学的人工肝臓
FR2690926B1 (fr) * 1992-05-06 1995-08-04 Lvmh Rech Dispositif du type reacteur a volume variable et procede de culture cellulaire.
US6858146B1 (en) 1992-09-11 2005-02-22 Xenogenics Artificial liver apparatus and method
WO1996039819A1 (en) * 1995-06-07 1996-12-19 St. Jude Medical, Inc. Cardiovascular bioreactor apparatus and method
US5827729A (en) * 1996-04-23 1998-10-27 Advanced Tissue Sciences Diffusion gradient bioreactor and extracorporeal liver device using a three-dimensional liver tissue
US5846829A (en) * 1996-11-08 1998-12-08 Board Of Trustees Operating Michigan State University Method and apparatus for growing plant cells
US6068775A (en) * 1998-04-13 2000-05-30 Circe Biomedical, Inc. Removal of agent from cell suspension
EP0950932B1 (fr) 1998-04-17 2004-06-30 Daniel Roth et Gerald Genta Haute Horlogerie SA Garde-temps dont un des affichages s'étend sur un arc de cercle
WO2000078932A1 (en) * 1999-06-21 2000-12-28 The General Hospital Corporation Cell culture systems and methods for organ assist devices
EP1234553A1 (de) * 2001-02-14 2002-08-28 Boris-Wolfgang Hochleitner Bioartifizielles Gerät
US6582955B2 (en) 2001-05-11 2003-06-24 Spectrum Laboratories, Inc. Bioreactor with application as blood therapy device
WO2005036125A2 (en) * 2003-09-10 2005-04-21 Triosyn Holding, Inc. System, method and apparatus for purifying biological fluids such as blood and constituents thereof
DE102004053164B4 (de) * 2004-11-01 2007-06-14 Innovent E.V. Technologieentwicklung Beschichtete Mikroträger, ein Verfahren zu ihrer Herstellung und ihre Verwendung
GB2467645B (en) * 2007-07-06 2011-12-21 Univ London A method of proliferating human hepatocyte cells

Also Published As

Publication number Publication date
ES2434218T3 (es) 2013-12-16
GB2467645A (en) 2010-08-11
EP2505220B1 (en) 2018-01-24
EP2170429A2 (en) 2010-04-07
US20160316742A1 (en) 2016-11-03
ES2666888T3 (es) 2018-05-08
US20110125286A1 (en) 2011-05-26
GB2450761A (en) 2009-01-07
NO2505220T3 (da) 2018-06-23
EP2170429B1 (en) 2013-08-14
EP2505220A3 (en) 2013-05-01
WO2009007678A2 (en) 2009-01-15
GB201001916D0 (en) 2010-03-24
US9402944B2 (en) 2016-08-02
GB0713595D0 (en) 2007-08-22
WO2009007678A3 (en) 2009-07-30
EP2505220A2 (en) 2012-10-03
GB2450761B (en) 2010-08-04
GB2467645B (en) 2011-12-21

Similar Documents

Publication Publication Date Title
DK2505220T3 (da) Fremgangsmåde til formering af humane hepatocytafledte celler
US9650609B2 (en) Bioartificial liver system
JP3692147B2 (ja) フィルターデバイス
TWI576433B (zh) Cell culture system and cell culture method
US8785181B2 (en) Cell expansion system and methods of use
Lazar et al. Formation of porcine hepatocyte spheroids for use in a bioartificial liver
US7122371B1 (en) Modular cell culture bioreactor
Jasmund et al. Cultivation of primary porcine hepatocytes in an OXY‐HFB for use as a bioartificial liver device
Sielaff et al. Characterization of the three-compartment gel-entrapment porcine hepatocyte bioartificial liver
JPH06509476A (ja) 生物学的人工肝臓
Catapano et al. Bioreactors for liver tissue engineering
Dermenoudis et al. Bioreactors in tissue engineering
JPH0870847A (ja) 細胞浮遊培養用容器
EP2376627B1 (en) Improvements in or relating to growing cells
AU2009201912B2 (en) Process for preserving insulin-secreting cells intended to be transplanted in a patient
Shi et al. A novel configuration of bioartificial liver support system based on circulating microcarrier culture
Goltry et al. Large-scale production of adult stem cells for clinical use
JP2005218368A (ja) 細胞培養装置
JPH0576364A (ja) 動物遊離細胞の固定化物、固定化方法および培養方法
US20230374433A1 (en) System and methods for immune cells expansion and activation in large scale
Park et al. Bioartificial liver
JPS5967965A (ja) 人工肝臓装置
De Bartolo et al. Membrane Bioartificial Organs