DK2414507T3 - Mutanter af aktiveringsinduceret cytidin-deaminase (aid) og til anvendelsesfremgangsmåder - Google Patents

Mutanter af aktiveringsinduceret cytidin-deaminase (aid) og til anvendelsesfremgangsmåder

Info

Publication number
DK2414507T3
DK2414507T3 DK10723293.6T DK10723293T DK2414507T3 DK 2414507 T3 DK2414507 T3 DK 2414507T3 DK 10723293 T DK10723293 T DK 10723293T DK 2414507 T3 DK2414507 T3 DK 2414507T3
Authority
DK
Denmark
Prior art keywords
deaminase
mutants
activation
aid
induced cytidine
Prior art date
Application number
DK10723293.6T
Other languages
Danish (da)
English (en)
Inventor
Meng Wang
Zizhen Yang
Cristina Rada
Michael Neuberger
Original Assignee
Medical Res Council
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Medical Res Council filed Critical Medical Res Council
Application granted granted Critical
Publication of DK2414507T3 publication Critical patent/DK2414507T3/da

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y305/00—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
    • C12Y305/04—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in cyclic amidines (3.5.4)
    • C12Y305/04005—Cytidine deaminase (3.5.4.5)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/23—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a GST-tag
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2799/00—Uses of viruses
    • C12N2799/02—Uses of viruses as vector
    • C12N2799/021—Uses of viruses as vector for the expression of a heterologous nucleic acid
    • C12N2799/027—Uses of viruses as vector for the expression of a heterologous nucleic acid where the vector is derived from a retrovirus

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DK10723293.6T 2009-04-03 2010-04-05 Mutanter af aktiveringsinduceret cytidin-deaminase (aid) og til anvendelsesfremgangsmåder DK2414507T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US16634909P 2009-04-03 2009-04-03
PCT/IB2010/000958 WO2010113039A1 (en) 2009-04-03 2010-04-05 Mutants of activation-induced cytidine deaminase (aid) and methods of use

Publications (1)

Publication Number Publication Date
DK2414507T3 true DK2414507T3 (da) 2014-08-25

Family

ID=42617524

Family Applications (1)

Application Number Title Priority Date Filing Date
DK10723293.6T DK2414507T3 (da) 2009-04-03 2010-04-05 Mutanter af aktiveringsinduceret cytidin-deaminase (aid) og til anvendelsesfremgangsmåder

Country Status (10)

Country Link
US (1) US9683226B2 (enExample)
EP (1) EP2414507B1 (enExample)
JP (1) JP5683566B2 (enExample)
CN (1) CN102482639B (enExample)
AU (1) AU2010230985B2 (enExample)
CA (1) CA2757178C (enExample)
DK (1) DK2414507T3 (enExample)
ES (1) ES2498765T3 (enExample)
RU (1) RU2537264C2 (enExample)
WO (1) WO2010113039A1 (enExample)

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CN107177625B (zh) * 2017-05-26 2021-05-25 中国农业科学院植物保护研究所 一种定点突变的人工载体系统及定点突变方法
US10961525B2 (en) * 2017-07-05 2021-03-30 The Trustees Of The University Of Pennsylvania Hyperactive AID/APOBEC and hmC dominant TET enzymes
CN109402096B (zh) * 2018-11-20 2021-01-01 中国科学院生物物理研究所 一种aid酶突变体及其应用
WO2020148206A1 (en) * 2019-01-14 2020-07-23 INSERM (Institut National de la Santé et de la Recherche Médicale) Methods and kits for generating and selecting a variant of a binding protein with increased binding affinity and/or specificity
WO2020206325A2 (en) * 2019-04-05 2020-10-08 The Broad Institute, Inc. A pseudo-random dna editor for efficient and continuous nucleotide diversification in human cells
CN110734900B (zh) * 2019-11-06 2022-09-30 上海科技大学 一种胞嘧啶碱基编辑工具及其用途
CN111518794B (zh) * 2020-04-13 2023-05-16 中山大学 基于激活诱导性胞苷脱氨酶的诱导突变蛋白的制备和用途
IL276599A (en) 2020-08-09 2022-03-01 Yeda Res & Dev T cell receptor unique to mage-a1 and its uses
CN112708650A (zh) * 2020-12-31 2021-04-27 河南巨龙生物工程股份有限公司 一种缩短胞苷发酵迟滞期的方法
CN113073094B (zh) * 2021-03-29 2023-03-28 中山大学 基于胞苷脱氨酶LjCDA1L1_4a及其突变体的单碱基突变系统
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Also Published As

Publication number Publication date
JP2012522501A (ja) 2012-09-27
EP2414507B1 (en) 2014-07-02
AU2010230985A1 (en) 2011-10-06
ES2498765T3 (es) 2014-09-25
WO2010113039A1 (en) 2010-10-07
CA2757178C (en) 2020-05-19
CN102482639A (zh) 2012-05-30
RU2011144569A (ru) 2013-05-10
RU2537264C2 (ru) 2014-12-27
AU2010230985B2 (en) 2015-09-24
JP5683566B2 (ja) 2015-03-11
CN102482639B (zh) 2016-01-06
US20120151613A1 (en) 2012-06-14
CA2757178A1 (en) 2010-10-07
EP2414507A1 (en) 2012-02-08
US9683226B2 (en) 2017-06-20

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