RU2537264C2 - Мутанты индуцируемой активацией цитидиндеаминазы (aid) и способы их применения - Google Patents

Мутанты индуцируемой активацией цитидиндеаминазы (aid) и способы их применения Download PDF

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RU2537264C2
RU2537264C2 RU2011144569/10A RU2011144569A RU2537264C2 RU 2537264 C2 RU2537264 C2 RU 2537264C2 RU 2011144569/10 A RU2011144569/10 A RU 2011144569/10A RU 2011144569 A RU2011144569 A RU 2011144569A RU 2537264 C2 RU2537264 C2 RU 2537264C2
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amino acid
residue
aid
acid substitution
nucleic acid
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RU2011144569/10A
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RU2011144569A (ru
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Мен ВАН
Зизхен ЯНГ
Кристина РАДА
Майкл НОЙБЕРГЕР
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Медикал Рисерч Каунсил
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y305/00—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
    • C12Y305/04—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in cyclic amidines (3.5.4)
    • C12Y305/04005—Cytidine deaminase (3.5.4.5)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/23—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a GST-tag
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2799/00—Uses of viruses
    • C12N2799/02—Uses of viruses as vector
    • C12N2799/021—Uses of viruses as vector for the expression of a heterologous nucleic acid
    • C12N2799/027—Uses of viruses as vector for the expression of a heterologous nucleic acid where the vector is derived from a retrovirus

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
RU2011144569/10A 2009-04-03 2010-04-05 Мутанты индуцируемой активацией цитидиндеаминазы (aid) и способы их применения RU2537264C2 (ru)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US16634909P 2009-04-03 2009-04-03
US61/166,349 2009-04-03
PCT/IB2010/000958 WO2010113039A1 (en) 2009-04-03 2010-04-05 Mutants of activation-induced cytidine deaminase (aid) and methods of use

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RU2011144569A RU2011144569A (ru) 2013-05-10
RU2537264C2 true RU2537264C2 (ru) 2014-12-27

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US (1) US9683226B2 (enExample)
EP (1) EP2414507B1 (enExample)
JP (1) JP5683566B2 (enExample)
CN (1) CN102482639B (enExample)
AU (1) AU2010230985B2 (enExample)
CA (1) CA2757178C (enExample)
DK (1) DK2414507T3 (enExample)
ES (1) ES2498765T3 (enExample)
RU (1) RU2537264C2 (enExample)
WO (1) WO2010113039A1 (enExample)

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PL225333B1 (pl) * 2013-01-14 2017-03-31 Inst Chemii Bioorganicznej Polskiej Akademii Nauk Preparat wykazujący aktywność ludzkiej deaminazy cytydyny indukowanej aktywacją limfocytów B (AID) oraz sposób otrzymywania tego preparatu w systemie bakteryjnym
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US11707056B2 (en) 2013-05-02 2023-07-25 Kymab Limited Animals, repertoires and methods
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CN104263755B (zh) * 2014-07-03 2017-02-15 成都中联生科基因科技有限公司 一种利用dna损伤修复机制使单克隆抗体细胞株进行体内快速进化的新方法
US10968444B2 (en) * 2017-05-22 2021-04-06 Abzyme Therapeutics Llc Methods for generating bispecific functional agents
US10704040B2 (en) * 2017-05-22 2020-07-07 Abzyme Therapeutics Llc Triple-mode system for antibody maturation, surface display and secretion
CN107177625B (zh) * 2017-05-26 2021-05-25 中国农业科学院植物保护研究所 一种定点突变的人工载体系统及定点突变方法
US10961525B2 (en) * 2017-07-05 2021-03-30 The Trustees Of The University Of Pennsylvania Hyperactive AID/APOBEC and hmC dominant TET enzymes
CN109402096B (zh) * 2018-11-20 2021-01-01 中国科学院生物物理研究所 一种aid酶突变体及其应用
WO2020148206A1 (en) * 2019-01-14 2020-07-23 INSERM (Institut National de la Santé et de la Recherche Médicale) Methods and kits for generating and selecting a variant of a binding protein with increased binding affinity and/or specificity
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CN110734900B (zh) * 2019-11-06 2022-09-30 上海科技大学 一种胞嘧啶碱基编辑工具及其用途
CN111518794B (zh) * 2020-04-13 2023-05-16 中山大学 基于激活诱导性胞苷脱氨酶的诱导突变蛋白的制备和用途
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CN112708650A (zh) * 2020-12-31 2021-04-27 河南巨龙生物工程股份有限公司 一种缩短胞苷发酵迟滞期的方法
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Publication number Publication date
DK2414507T3 (da) 2014-08-25
JP2012522501A (ja) 2012-09-27
EP2414507B1 (en) 2014-07-02
AU2010230985A1 (en) 2011-10-06
ES2498765T3 (es) 2014-09-25
WO2010113039A1 (en) 2010-10-07
CA2757178C (en) 2020-05-19
CN102482639A (zh) 2012-05-30
RU2011144569A (ru) 2013-05-10
AU2010230985B2 (en) 2015-09-24
JP5683566B2 (ja) 2015-03-11
CN102482639B (zh) 2016-01-06
US20120151613A1 (en) 2012-06-14
CA2757178A1 (en) 2010-10-07
EP2414507A1 (en) 2012-02-08
US9683226B2 (en) 2017-06-20

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