CN102482639B - 活化诱导胞苷脱氨酶(aid)突变体及使用方法 - Google Patents

活化诱导胞苷脱氨酶(aid)突变体及使用方法 Download PDF

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CN102482639B
CN102482639B CN201080023311.XA CN201080023311A CN102482639B CN 102482639 B CN102482639 B CN 102482639B CN 201080023311 A CN201080023311 A CN 201080023311A CN 102482639 B CN102482639 B CN 102482639B
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aid
amino acid
protein
residue
nucleic acid
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CN102482639A (zh
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M·王
Z·杨
C·拉达
M·纽伯格
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British Research And Innovation Agency
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y305/00—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
    • C12Y305/04—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in cyclic amidines (3.5.4)
    • C12Y305/04005—Cytidine deaminase (3.5.4.5)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/23—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a GST-tag
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2799/00—Uses of viruses
    • C12N2799/02—Uses of viruses as vector
    • C12N2799/021—Uses of viruses as vector for the expression of a heterologous nucleic acid
    • C12N2799/027—Uses of viruses as vector for the expression of a heterologous nucleic acid where the vector is derived from a retrovirus

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
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  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
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  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN201080023311.XA 2009-04-03 2010-04-05 活化诱导胞苷脱氨酶(aid)突变体及使用方法 Active CN102482639B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US16634909P 2009-04-03 2009-04-03
US61/166,349 2009-04-03
PCT/IB2010/000958 WO2010113039A1 (en) 2009-04-03 2010-04-05 Mutants of activation-induced cytidine deaminase (aid) and methods of use

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CN102482639A CN102482639A (zh) 2012-05-30
CN102482639B true CN102482639B (zh) 2016-01-06

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US (1) US9683226B2 (enExample)
EP (1) EP2414507B1 (enExample)
JP (1) JP5683566B2 (enExample)
CN (1) CN102482639B (enExample)
AU (1) AU2010230985B2 (enExample)
CA (1) CA2757178C (enExample)
DK (1) DK2414507T3 (enExample)
ES (1) ES2498765T3 (enExample)
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WO (1) WO2010113039A1 (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107177625A (zh) * 2017-05-26 2017-09-19 中国农业科学院植物保护研究所 一种定点突变的人工载体系统及定点突变方法

Families Citing this family (37)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9005974B2 (en) * 2005-12-09 2015-04-14 Academish Medisch Centrum Bij De Universiteit Van Amsterdam Means and methods for influencing the stability of cells
NZ600075A (en) * 2005-12-09 2013-12-20 Amc Amsterdam Means and methods for influencing the stability of antibody producing cells
DK3241435T3 (da) 2009-07-08 2021-08-23 Kymab Ltd Dyremodeller og terapeutiske molekyler
US9445581B2 (en) 2012-03-28 2016-09-20 Kymab Limited Animal models and therapeutic molecules
WO2011008093A1 (en) * 2009-07-15 2011-01-20 Aimm Therapeutics B.V. Means and methods for producing high affinity antibodies
WO2012063048A1 (en) 2010-11-08 2012-05-18 Kymab Limited Cells & vertebrates for enhanced somatic hypermutation and class switch recombination
WO2012072814A1 (en) 2010-12-02 2012-06-07 Aimm Therapeutics B.V. Means and methods for producing high affinity antibodies
WO2012104843A1 (en) 2011-02-06 2012-08-09 Yeda Research And Development Co.Ltd. At The Weizmann Institute Of Science Affinity maturated t cell receptors and use thereof
AU2012311286B2 (en) 2011-09-19 2018-07-26 Kymab Limited Antibodies, variable domains and chains tailored for human use
WO2013045916A1 (en) 2011-09-26 2013-04-04 Kymab Limited Chimaeric surrogate light chains (slc) comprising human vpreb
US9253965B2 (en) 2012-03-28 2016-02-09 Kymab Limited Animal models and therapeutic molecules
GB2502127A (en) 2012-05-17 2013-11-20 Kymab Ltd Multivalent antibodies and in vivo methods for their production
US10251377B2 (en) 2012-03-28 2019-04-09 Kymab Limited Transgenic non-human vertebrate for the expression of class-switched, fully human, antibodies
CN103031295B (zh) * 2012-12-10 2015-01-28 浙江工业大学 冬虫夏草胞苷脱氨酶、编码基因及其应用
PL225333B1 (pl) * 2013-01-14 2017-03-31 Inst Chemii Bioorganicznej Polskiej Akademii Nauk Preparat wykazujący aktywność ludzkiej deaminazy cytydyny indukowanej aktywacją limfocytów B (AID) oraz sposób otrzymywania tego preparatu w systemie bakteryjnym
US9788534B2 (en) 2013-03-18 2017-10-17 Kymab Limited Animal models and therapeutic molecules
US9783618B2 (en) 2013-05-01 2017-10-10 Kymab Limited Manipulation of immunoglobulin gene diversity and multi-antibody therapeutics
US9783593B2 (en) 2013-05-02 2017-10-10 Kymab Limited Antibodies, variable domains and chains tailored for human use
US11707056B2 (en) 2013-05-02 2023-07-25 Kymab Limited Animals, repertoires and methods
WO2015049517A2 (en) 2013-10-01 2015-04-09 Kymab Limited Animal models and therapeutic molecules
DK3099709T3 (da) 2014-01-31 2020-02-03 Aimm Therapeutics Bv Midler og fremgangsmåder til fremstilling af stabile antistoffer
CN104263755B (zh) * 2014-07-03 2017-02-15 成都中联生科基因科技有限公司 一种利用dna损伤修复机制使单克隆抗体细胞株进行体内快速进化的新方法
US10704040B2 (en) * 2017-05-22 2020-07-07 Abzyme Therapeutics Llc Triple-mode system for antibody maturation, surface display and secretion
US10968444B2 (en) * 2017-05-22 2021-04-06 Abzyme Therapeutics Llc Methods for generating bispecific functional agents
US10961525B2 (en) * 2017-07-05 2021-03-30 The Trustees Of The University Of Pennsylvania Hyperactive AID/APOBEC and hmC dominant TET enzymes
GB201710984D0 (en) 2017-07-07 2017-08-23 Kymab Ltd Cells, vertebrates, populations & methods
CN109402096B (zh) * 2018-11-20 2021-01-01 中国科学院生物物理研究所 一种aid酶突变体及其应用
WO2020148206A1 (en) * 2019-01-14 2020-07-23 INSERM (Institut National de la Santé et de la Recherche Médicale) Methods and kits for generating and selecting a variant of a binding protein with increased binding affinity and/or specificity
US20220170006A1 (en) * 2019-04-05 2022-06-02 The Broad Institute, Inc. A pseudo-random dna editor for efficient and continuous nucleotide diversification in human cells
CN110734900B (zh) * 2019-11-06 2022-09-30 上海科技大学 一种胞嘧啶碱基编辑工具及其用途
CN111518794B (zh) 2020-04-13 2023-05-16 中山大学 基于激活诱导性胞苷脱氨酶的诱导突变蛋白的制备和用途
IL276599A (en) 2020-08-09 2022-03-01 Yeda Res & Dev T cell receptor unique to mage-a1 and its uses
CN112708650A (zh) * 2020-12-31 2021-04-27 河南巨龙生物工程股份有限公司 一种缩短胞苷发酵迟滞期的方法
CN113073094B (zh) * 2021-03-29 2023-03-28 中山大学 基于胞苷脱氨酶LjCDA1L1_4a及其突变体的单碱基突变系统
CN115094127A (zh) * 2022-02-22 2022-09-23 中国科学院深圳先进技术研究院 一种原位检测蛋白质与脱氧核糖核苷酸结合位置的方法
WO2023169410A1 (zh) * 2022-03-08 2023-09-14 中国科学院遗传与发育生物学研究所 胞嘧啶脱氨酶及其在碱基编辑中的用途
WO2025119295A1 (zh) * 2023-12-05 2025-06-12 广州瑞风生物科技有限公司 脱氨酶、融合蛋白、核酸、药物组合物及其应用

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN85108240A (zh) * 1984-12-06 1986-07-02 武田药品工业株式会社 胞苷和/或脱氧胞苷的生产方法

Family Cites Families (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4737462A (en) 1982-10-19 1988-04-12 Cetus Corporation Structural genes, plasmids and transformed cells for producing cysteine depleted muteins of interferon-β
US4518584A (en) 1983-04-15 1985-05-21 Cetus Corporation Human recombinant interleukin-2 muteins
CA1293460C (en) 1985-10-07 1991-12-24 Brian Lee Sauer Site-specific recombination of dna in yeast
US5583024A (en) 1985-12-02 1996-12-10 The Regents Of The University Of California Recombinant expression of Coleoptera luciferase
GB8601597D0 (en) 1986-01-23 1986-02-26 Wilson R H Nucleotide sequences
US5221623A (en) 1986-07-22 1993-06-22 Boyce Thompson Institute For Plant Research, Inc. Use of bacterial luciferase structural genes for cloning and monitoring gene expression in microorganisms and for tagging and identification of genetically engineered organisms
US4937190A (en) 1987-10-15 1990-06-26 Wisconsin Alumni Research Foundation Translation enhancer
US5070012A (en) 1988-03-30 1991-12-03 The Board Of Trustees Of The Leland Stanford Junior University Monitoring of cells and trans-activating transcription elements
US5683888A (en) 1989-07-22 1997-11-04 University Of Wales College Of Medicine Modified bioluminescent proteins and their use
US5283179A (en) 1990-09-10 1994-02-01 Promega Corporation Luciferase assay method
ATE159548T1 (de) 1990-11-13 1997-11-15 Immunex Corp Bifunktionelle wählbare fusionsgene
WO1992015694A1 (en) 1991-03-08 1992-09-17 The Salk Institute For Biological Studies Flp-mediated gene modification in mammalian cells, and compositions and cells useful therefor
GB9116601D0 (en) 1991-08-01 1991-09-18 Iaf Biochem Int 1,3-oxathiolane nucleoside analogues
US5770428A (en) 1993-02-17 1998-06-23 Wisconsin Alumni Research Foundation Chimeric retrovial expression vectors and particles containing a simple retroviral long terminal repeat, BLV or HIV coding regions and cis-acting regulatory sequences, and an RNA translational enhancer with internal ribsome entry site
AU6953394A (en) 1993-05-21 1994-12-20 Targeted Genetics Corporation Bifunctional selectable fusion genes based on the cytosine deaminase (cd) gene
US5814618A (en) 1993-06-14 1998-09-29 Basf Aktiengesellschaft Methods for regulating gene expression
US5464758A (en) 1993-06-14 1995-11-07 Gossen; Manfred Tight control of gene expression in eucaryotic cells by tetracycline-responsive promoters
JP3466765B2 (ja) 1994-07-27 2003-11-17 キッコーマン株式会社 ビオチン化ホタルルシフェラーゼ、ビオチン化ホタルルシフェラーゼ遺伝子、新規な組み換え体ｄｎａ、ビオチン化ホタルルシフェラーゼの製造法及び生物発光分析法
US5741657A (en) 1995-03-20 1998-04-21 The Regents Of The University Of California Fluorogenic substrates for β-lactamase and methods of use
US5885827A (en) 1996-01-23 1999-03-23 The Regents Of The Universtiy Of California Eukaryotic high rate mutagenesis system
US5955604A (en) 1996-10-15 1999-09-21 The Regents Of The University Of California Substrates for β-lactamase and uses thereof
AU3330700A (en) * 1999-03-29 2000-10-16 Tasuku Honjo Novel cytidine deaminase
FR2814642B1 (fr) 2000-10-03 2005-07-01 Ass Pour Le Dev De La Rech En Souris transgenique pour la recombinaison ciblee mediee par la cre-er modifiee
US20040142325A1 (en) * 2001-09-14 2004-07-22 Liat Mintz Methods and systems for annotating biomolecular sequences
WO2003095636A2 (en) * 2002-05-10 2003-11-20 Medical Research Council Activation induced deaminase (aid)
US20050287648A1 (en) * 2002-08-05 2005-12-29 University Of Rochester Protein Transducing Domain/Deaminase Chimeric Proteins, Related Compounds, and Uses Thereof
US7393923B2 (en) 2004-11-08 2008-07-01 The Regents Of The University Of California Red-shifted fluorescent proteins mPlum and mRaspberry and polynucleotides encoding the same
JP4482693B2 (ja) 2005-08-29 2010-06-16 国立大学法人 岡山大学 抗体産生細胞の特異的選択方法
GB0607063D0 (en) * 2006-04-07 2006-05-17 Cellcentric Ltd Compositions and methods for epigenetic modification of nucleic acid sequences in vivo
JP5675109B2 (ja) 2007-02-20 2015-02-25 アナプティスバイオ インコーポレイティッド ライブラリーの生成方法及びその使用
US7803582B2 (en) * 2007-03-17 2010-09-28 Hongzhan Xu Recombinant vector and use in gene therapy
US8095350B2 (en) * 2007-12-21 2012-01-10 Xiaojiang Chen Three-dimensional structure of the APOBEC 2 structure, uses thereof, and methods for treating chronic and infectious diseases
US7981655B2 (en) * 2008-02-07 2011-07-19 Regents Of The University Of Minnesota Modified cytosine deaminases
EP2098536A1 (en) * 2008-03-05 2009-09-09 4-Antibody AG Isolation and identification of antigen- or ligand-specific binding proteins

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN85108240A (zh) * 1984-12-06 1986-07-02 武田药品工业株式会社 胞苷和/或脱氧胞苷的生产方法

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Impact of Phosphorylation and Phosphorylation-null Mutants on the Activity and Deamination Specificity of Activation-induced Cytidine Deaminase;Pham. P. et al.;《THE JOURNAL OF BIOLOGICAL CHEMISTRY》;20080620;第17428-17439页 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107177625A (zh) * 2017-05-26 2017-09-19 中国农业科学院植物保护研究所 一种定点突变的人工载体系统及定点突变方法
CN107177625B (zh) * 2017-05-26 2021-05-25 中国农业科学院植物保护研究所 一种定点突变的人工载体系统及定点突变方法

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JP2012522501A (ja) 2012-09-27
AU2010230985A1 (en) 2011-10-06
CN102482639A (zh) 2012-05-30
RU2537264C2 (ru) 2014-12-27
DK2414507T3 (da) 2014-08-25
US20120151613A1 (en) 2012-06-14
CA2757178C (en) 2020-05-19
EP2414507B1 (en) 2014-07-02
CA2757178A1 (en) 2010-10-07
EP2414507A1 (en) 2012-02-08
RU2011144569A (ru) 2013-05-10
WO2010113039A1 (en) 2010-10-07
ES2498765T3 (es) 2014-09-25
JP5683566B2 (ja) 2015-03-11
AU2010230985B2 (en) 2015-09-24
US9683226B2 (en) 2017-06-20

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