CN102482639B - 活化诱导胞苷脱氨酶(aid)突变体及使用方法 - Google Patents

活化诱导胞苷脱氨酶(aid)突变体及使用方法 Download PDF

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CN102482639B
CN102482639B CN201080023311.XA CN201080023311A CN102482639B CN 102482639 B CN102482639 B CN 102482639B CN 201080023311 A CN201080023311 A CN 201080023311A CN 102482639 B CN102482639 B CN 102482639B
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aid
amino acid
protein
residue
nucleic acid
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CN102482639A (zh
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M·王
Z·杨
C·拉达
M·纽伯格
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y305/00—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
    • C12Y305/04—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in cyclic amidines (3.5.4)
    • C12Y305/04005—Cytidine deaminase (3.5.4.5)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/23—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a GST-tag
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2799/00—Uses of viruses
    • C12N2799/02—Uses of viruses as vector
    • C12N2799/021—Uses of viruses as vector for the expression of a heterologous nucleic acid
    • C12N2799/027—Uses of viruses as vector for the expression of a heterologous nucleic acid where the vector is derived from a retrovirus

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  • Health & Medical Sciences (AREA)
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  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
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  • General Health & Medical Sciences (AREA)
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  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN201080023311.XA 2009-04-03 2010-04-05 活化诱导胞苷脱氨酶(aid)突变体及使用方法 Active CN102482639B (zh)

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US16634909P 2009-04-03 2009-04-03
US61/166,349 2009-04-03
PCT/IB2010/000958 WO2010113039A1 (en) 2009-04-03 2010-04-05 Mutants of activation-induced cytidine deaminase (aid) and methods of use

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CN102482639A CN102482639A (zh) 2012-05-30
CN102482639B true CN102482639B (zh) 2016-01-06

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EP (1) EP2414507B1 (enExample)
JP (1) JP5683566B2 (enExample)
CN (1) CN102482639B (enExample)
AU (1) AU2010230985B2 (enExample)
CA (1) CA2757178C (enExample)
DK (1) DK2414507T3 (enExample)
ES (1) ES2498765T3 (enExample)
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WO (1) WO2010113039A1 (enExample)

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US9445581B2 (en) 2012-03-28 2016-09-20 Kymab Limited Animal models and therapeutic molecules
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ES2626671T3 (es) 2010-12-02 2017-07-25 Aimm Therapeutics B.V. Medios y métodos para producir anticuerpos de alta afinidad
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US9253965B2 (en) 2012-03-28 2016-02-09 Kymab Limited Animal models and therapeutic molecules
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US10251377B2 (en) 2012-03-28 2019-04-09 Kymab Limited Transgenic non-human vertebrate for the expression of class-switched, fully human, antibodies
CN103031295B (zh) * 2012-12-10 2015-01-28 浙江工业大学 冬虫夏草胞苷脱氨酶、编码基因及其应用
PL225333B1 (pl) * 2013-01-14 2017-03-31 Inst Chemii Bioorganicznej Polskiej Akademii Nauk Preparat wykazujący aktywność ludzkiej deaminazy cytydyny indukowanej aktywacją limfocytów B (AID) oraz sposób otrzymywania tego preparatu w systemie bakteryjnym
US9788534B2 (en) 2013-03-18 2017-10-17 Kymab Limited Animal models and therapeutic molecules
US9783618B2 (en) 2013-05-01 2017-10-10 Kymab Limited Manipulation of immunoglobulin gene diversity and multi-antibody therapeutics
US9783593B2 (en) 2013-05-02 2017-10-10 Kymab Limited Antibodies, variable domains and chains tailored for human use
US11707056B2 (en) 2013-05-02 2023-07-25 Kymab Limited Animals, repertoires and methods
CA2925723A1 (en) 2013-10-01 2015-04-09 Kymab Limited Animal models and therapeutic molecules
WO2015115892A1 (en) 2014-01-31 2015-08-06 Aimm Therapeutics B.V. Means and methods for producing stable antibodies
CN104263755B (zh) * 2014-07-03 2017-02-15 成都中联生科基因科技有限公司 一种利用dna损伤修复机制使单克隆抗体细胞株进行体内快速进化的新方法
US10968444B2 (en) * 2017-05-22 2021-04-06 Abzyme Therapeutics Llc Methods for generating bispecific functional agents
US10704040B2 (en) * 2017-05-22 2020-07-07 Abzyme Therapeutics Llc Triple-mode system for antibody maturation, surface display and secretion
US10961525B2 (en) 2017-07-05 2021-03-30 The Trustees Of The University Of Pennsylvania Hyperactive AID/APOBEC and hmC dominant TET enzymes
CN109402096B (zh) * 2018-11-20 2021-01-01 中国科学院生物物理研究所 一种aid酶突变体及其应用
EP3911746A1 (en) * 2019-01-14 2021-11-24 Institut National de la Santé et de la Recherche Médicale (INSERM) Methods and kits for generating and selecting a variant of a binding protein with increased binding affinity and/or specificity
US20220170006A1 (en) * 2019-04-05 2022-06-02 The Broad Institute, Inc. A pseudo-random dna editor for efficient and continuous nucleotide diversification in human cells
CN110734900B (zh) * 2019-11-06 2022-09-30 上海科技大学 一种胞嘧啶碱基编辑工具及其用途
CN111518794B (zh) * 2020-04-13 2023-05-16 中山大学 基于激活诱导性胞苷脱氨酶的诱导突变蛋白的制备和用途
IL276599A (en) 2020-08-09 2022-03-01 Yeda Res & Dev T cell receptor unique to mage-a1 and its uses
CN112708650A (zh) * 2020-12-31 2021-04-27 河南巨龙生物工程股份有限公司 一种缩短胞苷发酵迟滞期的方法
CN113073094B (zh) * 2021-03-29 2023-03-28 中山大学 基于胞苷脱氨酶LjCDA1L1_4a及其突变体的单碱基突变系统
CN115094127A (zh) * 2022-02-22 2022-09-23 中国科学院深圳先进技术研究院 一种原位检测蛋白质与脱氧核糖核苷酸结合位置的方法
EP4491723A1 (en) * 2022-03-08 2025-01-15 Institute Of Genetics And Developmental Biology, Chinese Academy Of Sciences Cytosine deaminase and use thereof in base editing
WO2025119295A1 (zh) * 2023-12-05 2025-06-12 广州瑞风生物科技有限公司 脱氨酶、融合蛋白、核酸、药物组合物及其应用

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Publication number Priority date Publication date Assignee Title
CN107177625A (zh) * 2017-05-26 2017-09-19 中国农业科学院植物保护研究所 一种定点突变的人工载体系统及定点突变方法
CN107177625B (zh) * 2017-05-26 2021-05-25 中国农业科学院植物保护研究所 一种定点突变的人工载体系统及定点突变方法

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RU2011144569A (ru) 2013-05-10
DK2414507T3 (da) 2014-08-25
JP5683566B2 (ja) 2015-03-11
US20120151613A1 (en) 2012-06-14
AU2010230985A1 (en) 2011-10-06
WO2010113039A1 (en) 2010-10-07
EP2414507B1 (en) 2014-07-02
EP2414507A1 (en) 2012-02-08
ES2498765T3 (es) 2014-09-25
CA2757178C (en) 2020-05-19
US9683226B2 (en) 2017-06-20
CA2757178A1 (en) 2010-10-07
RU2537264C2 (ru) 2014-12-27
CN102482639A (zh) 2012-05-30
JP2012522501A (ja) 2012-09-27
AU2010230985B2 (en) 2015-09-24

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