DK2258831T3 - Gærmutant og gærekstrakt - Google Patents

Gærmutant og gærekstrakt Download PDF

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Publication number
DK2258831T3
DK2258831T3 DK09727399.9T DK09727399T DK2258831T3 DK 2258831 T3 DK2258831 T3 DK 2258831T3 DK 09727399 T DK09727399 T DK 09727399T DK 2258831 T3 DK2258831 T3 DK 2258831T3
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DK
Denmark
Prior art keywords
yeast
acid
glutamic acid
weight
yeast extract
Prior art date
Application number
DK09727399.9T
Other languages
English (en)
Inventor
Ryo Iwakiri
Hirokazu Maekawa
Shogo Furue
Hiroko Kodera
Setsuko Hirakura
Masahiro Nishida
Masanori Uchida
Sakiko Ikeda
Naohisa Masuo
Original Assignee
Kohjin Life Sciences Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kohjin Life Sciences Co Ltd filed Critical Kohjin Life Sciences Co Ltd
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Publication of DK2258831T3 publication Critical patent/DK2258831T3/da

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Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L31/00—Edible extracts or preparations of fungi; Preparation or treatment thereof
    • A23L31/10—Yeasts or derivatives thereof
    • A23L31/15—Extracts
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/10—Natural spices, flavouring agents or condiments; Extracts thereof
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/14—Yeasts or derivatives thereof
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/14—Yeasts or derivatives thereof
    • A23L33/145—Extracts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/165—Yeast isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/01—Preparation of mutants without inserting foreign genetic material therein; Screening processes therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/14—Glutamic acid; Glutamine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/32—Nucleotides having a condensed ring system containing a six-membered ring having two N-atoms in the same ring, e.g. purine nucleotides, nicotineamide-adenine dinucleotide
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/72—Candida

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Mycology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Nutrition Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • Botany (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Seasonings (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Claims (8)

1. Gærmutant, hvor gærmutanten er Candida utilis 36D61 (accessionsnummer: FERM BP-11103).
2. Fremgangsmåde til fremstilling af et gærekstrakt fra gærmutanten ifølge krav 1, hvor gærekstraktet indeholder L-glutaminsyre i en mængde på 20 vægtprocent eller mere omdannet til en mængde af et natriumsalt deraf.
3. Fremgangsmåden ifølge krav 2, hvor gærekstraktet yderligere indeholder 5'-guanylsyre og 5'-inosinsyre eller 5'-adenylsyre i en total mængde på 3 vægtprocent eller mere.
4. Fremgangsmåde til fremstilling af en gær, der akkumulerer fri glutaminsyre og glutamin i en total mængde på 10 % eller mere og ribonukleinsyre (RNA) i en mængde på 5 % eller mere, baseret på en vægt afen tør celle, ved (a) at anvende et mutageniserende middel og (b) at vælge en streng i stand til at vokse på et synetisk medium indeholdende ethylbromopyruvat og 2-oxoglutarsyre i en koncentration ved hvilken moderstrengen dertil ikke kan vokse.
5. Fremgangsmåden ifølge krav 4, hvor gærmutanten er til at fremstille et gærekstrakt indeholdende glutaminsyre der udviser en stærk smag.
6. Fremgangsmåden ifølge et hvilket som helst af kravene 4 eller 5, hvor det mutageniserende middel vælges fra gruppen bestående af ultraviolette stråler, røntgenstråler, salpetersyrling, nitrosoguanidin og ethylmethansulfonat.
7. Fremgangsmåden ifølge et hvilket som helst af kravene 4 til 6, hvor gæren er en Candida utilis-gær.
8. Fremgangsmåden ifølge et hvilket som helst af kravene 4 til 7, hvor mutageniserings-behandlingen gentages.
DK09727399.9T 2008-03-31 2009-03-26 Gærmutant og gærekstrakt DK2258831T3 (da)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2008091709 2008-03-31
JP2008111232A JP5637507B2 (ja) 2008-03-31 2008-04-22 酵母変異株と酵母エキス
PCT/JP2009/056168 WO2009123019A1 (ja) 2008-03-31 2009-03-26 酵母変異株と酵母エキス

Publications (1)

Publication Number Publication Date
DK2258831T3 true DK2258831T3 (da) 2017-09-18

Family

ID=41135398

Family Applications (1)

Application Number Title Priority Date Filing Date
DK09727399.9T DK2258831T3 (da) 2008-03-31 2009-03-26 Gærmutant og gærekstrakt

Country Status (8)

Country Link
US (3) US9084435B2 (da)
EP (1) EP2258831B1 (da)
JP (1) JP5637507B2 (da)
CN (1) CN101981179B (da)
AU (1) AU2009232936B2 (da)
DK (1) DK2258831T3 (da)
WO (1) WO2009123019A1 (da)
ZA (1) ZA201004611B (da)

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JP4503700B1 (ja) 2008-11-18 2010-07-14 アサヒビール株式会社 グルタミン酸高含有酵母の製造方法
WO2011074359A1 (ja) * 2009-12-17 2011-06-23 キリン協和フーズ株式会社 アルギニン高含有酵母エキスおよびその製造方法
JP6080191B2 (ja) * 2010-01-08 2017-02-15 興人ライフサイエンス株式会社 コク味を増強する調味料の製造方法
EP2620060B1 (en) * 2010-09-24 2020-02-12 Asahi Group Holdings, Ltd. Method for producing thermally reacted seasoning
FR2972728B1 (fr) * 2011-03-18 2015-08-07 Lesaffre & Cie Amelioration des panifications a fort taux de levure
JP2012196188A (ja) * 2011-03-23 2012-10-18 Kohjin Co Ltd コク味を増強する調味料の製造方法
CN103717093B (zh) * 2011-08-26 2016-03-30 兴人生命科学株式会社 具有味道增强效果的酵母抽提物
GB201118480D0 (en) * 2011-10-26 2011-12-07 Givaudan Sa Organic compounds
JP6630948B2 (ja) * 2012-10-15 2020-01-15 三菱商事ライフサイエンス株式会社 酵母タンパク由来調味料
WO2013146465A1 (ja) * 2012-03-30 2013-10-03 オリエンタル酵母工業株式会社 銅高含有酵母抽出物及びその製造方法、並びに、食品、及び野菜の緑色保持復元剤
KR101572140B1 (ko) 2013-10-08 2015-11-27 주식회사 태경식품 핵산 함량이 증대된 효모 변이주 및 이의 배양방법
KR101581616B1 (ko) * 2015-01-29 2015-12-30 최복이 죽요리용 육수원료분말의 제조방법
FR3034101B1 (fr) * 2015-03-24 2019-07-12 Lesaffre Et Compagnie Extrait de levure et son utilisation pour le collage de mouts et de boissons
US10827771B2 (en) 2015-04-28 2020-11-10 Tablemark Co., Ltd. Method for producing yeast extract, yeast extract obtained thereby, seasoning composition, and food
JP6172814B2 (ja) * 2015-05-15 2017-08-02 ヤマサ醤油株式会社 高いリボ核酸収量を示す酵母の製造方法
WO2017209175A1 (ja) * 2016-05-31 2017-12-07 キッコーマン株式会社 核酸含有発酵調味料及びその製造方法
JP6906348B2 (ja) * 2017-04-10 2021-07-21 三菱商事ライフサイエンス株式会社 酵母の改良方法
JP6886191B2 (ja) * 2018-12-14 2021-06-16 ヤマサ醤油株式会社 リボ核酸高収量を示す酵母
WO2021119390A1 (en) * 2019-12-13 2021-06-17 Magna International Inc. Porous spreader assisted jet and spray impingement cooling systems
WO2023198506A1 (en) * 2022-04-15 2023-10-19 Dsm Ip Assets B.V. Yeast extract with free glutamate and 5'-ribonucleotides

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Also Published As

Publication number Publication date
WO2009123019A9 (ja) 2010-02-18
EP2258831A1 (en) 2010-12-08
US20110020528A1 (en) 2011-01-27
US20160331015A1 (en) 2016-11-17
JP5637507B2 (ja) 2014-12-10
CN101981179A (zh) 2011-02-23
WO2009123019A1 (ja) 2009-10-08
AU2009232936A1 (en) 2009-10-08
EP2258831A4 (en) 2012-09-19
JP2009261253A (ja) 2009-11-12
EP2258831B1 (en) 2017-07-05
AU2009232936B2 (en) 2014-11-06
US9084435B2 (en) 2015-07-21
CN101981179B (zh) 2015-07-29
ZA201004611B (en) 2011-09-28
US20150225690A1 (en) 2015-08-13

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