DK2240570T3 - Bakteriel ghostproduktionsproces (BG) under anvendelse af betapropiolacton (BLP) til slutinaktivering - Google Patents
Bakteriel ghostproduktionsproces (BG) under anvendelse af betapropiolacton (BLP) til slutinaktivering Download PDFInfo
- Publication number
- DK2240570T3 DK2240570T3 DK09702047.3T DK09702047T DK2240570T3 DK 2240570 T3 DK2240570 T3 DK 2240570T3 DK 09702047 T DK09702047 T DK 09702047T DK 2240570 T3 DK2240570 T3 DK 2240570T3
- Authority
- DK
- Denmark
- Prior art keywords
- bacterial
- gene
- ghost
- enzyme
- bacterial cells
- Prior art date
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/02—Bacterial antigens
- A61K39/025—Enterobacteriales, e.g. Enterobacter
- A61K39/0258—Escherichia
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/02—Bacterial antigens
- A61K39/025—Enterobacteriales, e.g. Enterobacter
- A61K39/0283—Shigella
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/06—Lysis of microorganisms
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
- A61K2039/52—Bacterial cells; Fungal cells; Protozoal cells
- A61K2039/521—Bacterial cells; Fungal cells; Protozoal cells inactivated (killed)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/555—Medicinal preparations containing antigens or antibodies characterised by a specific combination antigen/adjuvant
- A61K2039/55588—Adjuvants of undefined constitution
- A61K2039/55594—Adjuvants of undefined constitution from bacteria
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Organic Chemistry (AREA)
- Public Health (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- Veterinary Medicine (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Immunology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Epidemiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Oncology (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Communicable Diseases (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Claims (17)
1. Fremgangsmåde til fremstilling af en bakteriel g hostf re msti 11 i ng omfattende at behandle nævnte bakterielle ghost med betapropiolacton.
2. Fremgangsmåde ifølge krav 1 omfattende trinnene: (a) produktion af en bakteriel ghosttilberedning, og (b) efterfølgende behandling af den bakterielle ghosttilberedning med betapropiolacton ved betingelser, hvor antallet af eventuelle tilbageværende levende bakterieceller i nævnte ghosttilberedning er reduceret.
3. Fremgangsmåde ifølge krav 1 eller 2, hvor antallet af eventuelle tilbageværende levende bakterielle celler i nævnte ghosttilberedning er reduceret med mindst 103 - 104.
4. Fremgangsmåde ifølge krav 1 - 3, hvor de bakterielle ghost er behandlet med 0,01 -1 volumen% betapropiolacton som slutkoncentration.
5. Fremgangsmåde ifølge krav 1 - 4, hvor betapropiolactontilsætning udføres i to konsekutive trin.
6. Fremgangsmåde ifølge krav 1 - 5, hvor betapropiolactontilsætningen udføres ved 26 - 50°C, og hvor den bakterielle ghost inaktiveres i 10 - 60 minutter.
7. Fremgangsmåde ifølge et vilkårligt af kravene 1 - 6, hvor den bakterielle ghostfremstilling er opnået ved: (a) tilvejebringelse af bakterielle celler omfattende et gen, som koder for et lytisk protein i stand til at danne en tunnelstruktur i det bakterielle cellekappes kompleks, (b) udsættelse af den bakterielle celle for tilstande, hvor det lytiske gen udtrykkes, og de cytoplasmiske komponenter af de bakterielle celler frisættes, og (c) opnåelse af de resulterende bakterielle ghostbakterier.
8. Fremgangsmåde ifølge et vilkårligt af kravene 1 - 6. hvor den bakterielle ghosttilberedning er opnået ved: (a) tilvejebringelse af bakterielle celler omfattende et gen, som koder for et lytisk protein i stand til at danne en tunnelstruktur i det bakterielle cellekap-pekompleks, (b) dyrkning af de bakterielle celler ved tilstande, hvor det lytiske gen ikke udtrykkes, (c) udsættelse af den bakterielle celle for betingelser, hvor det lytiske gen udtrykkes, og de cytoplasmiske komponenter af de bakterielle celler frisættes, og (d) opnåelse af den resulterende bakterielle ghost.
9. Fremgangsmåde ifølge krav 7 eller 8, hvor de bakterielle celler yderligere koder for et enzym i stand til at hydrolysere cytoplasmiske komponenter i den bakterielle celle omfattende følgende trin: udsættelse af den bakterielle celle for betingelser, hvor enzymgenet udtrykkes, og de cytoplasmiske komponenter af de bakterielle celler nedbrydes.
10. Fremgangsmåde ifølge krav 7 eller 8, hvor de bakterielle celler yderligere koder for et enzym i stand til at hydrolysere cytoplasmiske komponenter i bakterievæggen, omfattende følgende trin: (a) eventuelt dyrkning af de bakterielle celler under tilstande, hvor enzymgenet ikke udtrykkes, (b) udsættelse af den bakterielle celle for betingelser, hvor enzymgenet udtrykkes, og de cytoplasmiske komponenter i de bakterielle celler nedbrydes.
11. Fremgangsmåde ifølge krav 9 eller 10, hvor genet, som koder for det hy-drolytiske enzym, er et nucleasegen.
12. Fremgangsmåde ifølge et vilkårligt af kravene 7-11, hvor det lytiske gen og enzymgenet er i operativ forbindelse med en regulatorisk dygtig ekspressionskontrolsekvens, hvor det lytiske gen og enzymgenet er på én eller flere vektorer.
13. Fremgangsmåde ifølge krav 12, hvor ekspressionen af det lytiske gen og ekspressionen af enzymet induceres til forskellige tider.
14. Farmaceutisk sammensætning omfattende et betapropiolactonbehandlet bakteriel ghosttilberedning og en farmaceutisk acceptabel bærer, fortyndingsmiddel og/eller adjuvans.
15. Sammensætning ifølge krav 14, som er en vaccine eller er en adjuvans.
16. Sammensætning ifølge krav 15 til anvendelse i human medicin eller til anvendelse i veterinær medicin.
17. Anvendelse af betapropiolacton ved fremstilling af en bakteriel ghosttilberedning.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US2197708P | 2008-01-18 | 2008-01-18 | |
| PCT/EP2009/000272 WO2009090093A1 (en) | 2008-01-18 | 2009-01-16 | Bacterial ghost (bg) production process using betapropiolactone (blp) for final inactivation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK2240570T3 true DK2240570T3 (da) | 2014-03-10 |
Family
ID=40532591
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK09702047.3T DK2240570T3 (da) | 2008-01-18 | 2009-01-16 | Bakteriel ghostproduktionsproces (BG) under anvendelse af betapropiolacton (BLP) til slutinaktivering |
Country Status (13)
| Country | Link |
|---|---|
| US (1) | US7968323B2 (da) |
| EP (1) | EP2240570B1 (da) |
| CN (1) | CN102224237B (da) |
| AU (1) | AU2009204952B2 (da) |
| CA (1) | CA2710814C (da) |
| DK (1) | DK2240570T3 (da) |
| ES (1) | ES2452471T3 (da) |
| NZ (1) | NZ586669A (da) |
| PL (1) | PL2240570T3 (da) |
| PT (1) | PT2240570E (da) |
| RU (1) | RU2520082C2 (da) |
| SI (1) | SI2240570T1 (da) |
| WO (1) | WO2009090093A1 (da) |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101934072B (zh) * | 2010-03-24 | 2012-05-23 | 天津农学院 | 一种制备App菌影的方法及App菌影装载巴氏杆菌抗原制备亚单位疫苗的方法 |
| CN101991863B (zh) * | 2010-04-28 | 2012-11-07 | 中国人民解放军军事医学科学院野战输血研究所 | 一种双重靶向dna疫苗及其构建方法 |
| EP2591798B1 (en) | 2011-11-09 | 2014-11-19 | Werner Lubitz | Vaccine for use in tumor immunotherapy |
| CN103436550B (zh) * | 2013-07-26 | 2015-04-15 | 黑龙江八一农垦大学 | 一种菌影载体的构建方法 |
| CN105143441B (zh) * | 2014-01-13 | 2019-11-08 | 张明杰 | 用带有活性因子的细胞空壳作为淋巴细胞体外培养增效剂的制备及其应用方法 |
| US10526574B2 (en) | 2014-02-21 | 2020-01-07 | Bird-C Gmbh & Co. Kg | Fed-batch process for the production of bacterial ghosts |
| EP3195878A1 (en) | 2016-01-22 | 2017-07-26 | Werner Lubitz | Bacterial ghosts for the treatment of cancer |
| KR101825439B1 (ko) | 2016-04-15 | 2018-02-05 | 배재대학교 산학협력단 | 염산 처리에 의한 그람양성 박테리아 고스트의 제조 방법 |
| WO2019081625A1 (en) | 2017-10-25 | 2019-05-02 | Allero Therapeutics Bvba | TREATMENT OF IMMUNE DISEASES BY ADMINISTRATION OF ANTIGEN-SPECIFIC FORMULATIONS |
| KR102178334B1 (ko) | 2019-01-29 | 2020-11-12 | 배재대학교 산학협력단 | 황산을 이용하는 박테리아 고스트 제조방법, 상기 방법으로 제조된 박테리아 고스트 및 이의 용도 |
| CN110812473A (zh) * | 2019-12-10 | 2020-02-21 | 广东君睿生物技术研究有限公司 | 一种副猪嗜血杆菌病、猪链球菌病和猪多杀性巴氏杆菌病三联灭活疫苗及其制备方法 |
| CN110951666A (zh) * | 2019-12-27 | 2020-04-03 | 江苏农牧科技职业学院 | 一种双蛋白表达的大肠杆菌菌蜕及其制备方法 |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US2989435A (en) | 1957-01-03 | 1961-06-20 | Philips Corp | Method of chemical sterilization of aqueous liquids containing bacteria or spores ofbacteria |
| DE3715840A1 (de) | 1987-05-12 | 1988-12-01 | Boehringer Mannheim Gmbh | Fuer ein lytisch wirksames, chimaeres protein kodierende rekombinante dna und ihre verwendung |
| DE4005874A1 (de) | 1990-02-24 | 1991-11-07 | Boehringer Mannheim Gmbh | Traegergebundene rekombinante proteine, verfahren zur herstellung und verwendung als immunogene und vakzine |
| BR9105978A (pt) | 1990-03-07 | 1992-11-17 | Engelhard Corp | Aditivo de forragem particulado,e composicao de forragem |
| ES2186694T3 (es) | 1990-06-07 | 2003-05-16 | Bayer Ag | Vacuna bacteriana para el tratamiento de necrosis hepatica causada por f. necrophorum. |
| RU2059417C1 (ru) * | 1992-10-01 | 1996-05-10 | Данилевич Василий Николаевич | Способ стерилизации питательных сред |
| DE10003241A1 (de) | 2000-01-26 | 2001-08-02 | Werner Lubitz | Verschließen von Bakterienghosts |
| ATE462779T1 (de) * | 2001-07-11 | 2010-04-15 | Lubitz Werner Prof Dr | Nukleinsäurefreie ghost zusammensetzungen |
| WO2005001059A2 (en) * | 2003-06-23 | 2005-01-06 | Novasterilis Inc. | Inactivating organisms using carbon dioxide at or near its supercritical pressure and temperature conditions |
| DE10335796A1 (de) | 2003-08-05 | 2005-03-03 | Lubitz, Werner, Prof. Dr. | Verschliessen von Bakterienghosts durch bioaffine Wechselwirkungen |
-
2009
- 2009-01-16 RU RU2010134400/10A patent/RU2520082C2/ru not_active IP Right Cessation
- 2009-01-16 CA CA2710814A patent/CA2710814C/en active Active
- 2009-01-16 PL PL09702047T patent/PL2240570T3/pl unknown
- 2009-01-16 DK DK09702047.3T patent/DK2240570T3/da active
- 2009-01-16 WO PCT/EP2009/000272 patent/WO2009090093A1/en active Application Filing
- 2009-01-16 PT PT97020473T patent/PT2240570E/pt unknown
- 2009-01-16 CN CN200980102422.7A patent/CN102224237B/zh not_active Expired - Fee Related
- 2009-01-16 AU AU2009204952A patent/AU2009204952B2/en not_active Ceased
- 2009-01-16 SI SI200930872T patent/SI2240570T1/sl unknown
- 2009-01-16 EP EP09702047.3A patent/EP2240570B1/en active Active
- 2009-01-16 ES ES09702047.3T patent/ES2452471T3/es active Active
- 2009-01-16 NZ NZ586669A patent/NZ586669A/en not_active IP Right Cessation
- 2009-01-21 US US12/356,703 patent/US7968323B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CN102224237B (zh) | 2014-03-12 |
| RU2010134400A (ru) | 2012-02-27 |
| EP2240570A1 (en) | 2010-10-20 |
| CA2710814C (en) | 2014-10-14 |
| SI2240570T1 (sl) | 2014-04-30 |
| CN102224237A (zh) | 2011-10-19 |
| PL2240570T3 (pl) | 2014-05-30 |
| ES2452471T3 (es) | 2014-04-01 |
| RU2520082C2 (ru) | 2014-06-20 |
| PT2240570E (pt) | 2014-02-17 |
| AU2009204952B2 (en) | 2013-02-07 |
| WO2009090093A1 (en) | 2009-07-23 |
| US7968323B2 (en) | 2011-06-28 |
| AU2009204952A1 (en) | 2009-07-23 |
| NZ586669A (en) | 2012-06-29 |
| EP2240570B1 (en) | 2013-12-11 |
| US20100092516A1 (en) | 2010-04-15 |
| CA2710814A1 (en) | 2009-07-23 |
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