DK176036B1 - Ekspression af biologisk aktive PDGF analoger i eukaroyte celler - Google Patents

Ekspression af biologisk aktive PDGF analoger i eukaroyte celler Download PDF

Info

Publication number
DK176036B1
DK176036B1 DK198704217A DK421787A DK176036B1 DK 176036 B1 DK176036 B1 DK 176036B1 DK 198704217 A DK198704217 A DK 198704217A DK 421787 A DK421787 A DK 421787A DK 176036 B1 DK176036 B1 DK 176036B1
Authority
DK
Denmark
Prior art keywords
chain
amino acid
pdgf
human pdgf
dna
Prior art date
Application number
DK198704217A
Other languages
Danish (da)
English (en)
Other versions
DK421787A (da
DK421787D0 (da
Inventor
Mark J Murray
James D Kelly
Original Assignee
Zymogenetics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US06/896,485 external-priority patent/US4766073A/en
Priority claimed from US06/942,484 external-priority patent/US4889919A/en
Application filed by Zymogenetics Inc filed Critical Zymogenetics Inc
Publication of DK421787D0 publication Critical patent/DK421787D0/da
Publication of DK421787A publication Critical patent/DK421787A/da
Application granted granted Critical
Publication of DK176036B1 publication Critical patent/DK176036B1/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/49—Platelet-derived growth factor [PDGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/11011—Alpharetrovirus, e.g. avian leucosis virus
    • C12N2740/11022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Mycology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Animal Behavior & Ethology (AREA)
  • Plant Pathology (AREA)
  • Toxicology (AREA)
  • Public Health (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Physics & Mathematics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Virology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
DK198704217A 1986-08-13 1987-08-12 Ekspression af biologisk aktive PDGF analoger i eukaroyte celler DK176036B1 (da)

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
US06/896,485 US4766073A (en) 1985-02-25 1986-08-13 Expression of biologically active PDGF analogs in eucaryotic cells
US89648586 1986-08-13
US94248486 1986-12-15
US06/942,161 US4845075A (en) 1985-02-25 1986-12-15 Biologically active B-chain homodimers
US06/942,484 US4889919A (en) 1986-08-13 1986-12-15 Biologically active PDGF derived A-chain homodimers
US94197086 1986-12-15
US94216186 1986-12-15
US06/941,970 US4849407A (en) 1986-08-13 1986-12-15 Biologically active mosaic proteins

Publications (3)

Publication Number Publication Date
DK421787D0 DK421787D0 (da) 1987-08-12
DK421787A DK421787A (da) 1988-02-14
DK176036B1 true DK176036B1 (da) 2006-01-09

Family

ID=27505982

Family Applications (1)

Application Number Title Priority Date Filing Date
DK198704217A DK176036B1 (da) 1986-08-13 1987-08-12 Ekspression af biologisk aktive PDGF analoger i eukaroyte celler

Country Status (7)

Country Link
EP (1) EP0259632B1 (fr)
JP (1) JPH085797B2 (fr)
AT (1) ATE131532T1 (fr)
AU (1) AU7681687A (fr)
CA (1) CA1341398C (fr)
DE (1) DE3751632T2 (fr)
DK (1) DK176036B1 (fr)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1988007057A1 (fr) * 1987-03-13 1988-09-22 Amgen Inc. Facteur de croissance purifie de derive de plaquette et ses procedes de purification
DE3855504T2 (de) * 1987-04-22 1997-01-23 Chiron Corp Rekombinante Herstellung von A-Ketten-Polypeptiden von PDGF
US6939849B1 (en) 1987-04-22 2005-09-06 Chiron Corporation Recombinant PDGF A-chain homodimers and methods of use
EP0422125B1 (fr) * 1988-06-30 1996-10-23 Cor Therapeutics, Inc. Peptides bloquant des plaquettes
EP0366816A1 (fr) * 1988-10-31 1990-05-09 Rorer Biotechnology Inc. Procédé de préparation par génie génétique de la chaîne A du facteur de croissance provenant de plaquettes sanguines de cellules endothéliates
US5968778A (en) * 1989-01-12 1999-10-19 Jurgen Hoppe PDGF-AB, preparation process and pharmaceuticals containing them
DE3900770A1 (de) * 1989-01-12 1990-07-26 Juergen Prof Dr Hoppe Verfahren zur herstellung von pdgf-a und pdgf-aa
IL96682A0 (en) * 1989-12-15 1991-09-16 Amgen Inc Production of biologically active platelet-derived growth factor from high expression host cell systems
US5272064A (en) * 1989-12-19 1993-12-21 Amgen Inc. DNA molecules encoding platelet-derived growth factor B chain analogs and method for expression thereof
WO1992001716A1 (fr) * 1990-07-23 1992-02-06 Zymogenetics, Inc. Facteur de croissance provenant des plaquettes humaines (pdgf) resistant aux proteases et procedes d'utilisation
WO1992011364A1 (fr) * 1990-12-21 1992-07-09 Creative Biomolecules, Inc. Antagonistes biosynthetiques de facteur de croissance plaquettaire
GB9101645D0 (en) * 1991-01-25 1991-03-06 British Bio Technology Compounds
AU3816593A (en) * 1992-03-30 1993-11-08 Schering Corporation Monomeric platelet-derived growth factor and prevention of stenosis or restenosis
WO1993025576A2 (fr) * 1992-06-05 1993-12-23 Sri International Peptides ayant un facteur de croissance derive des plaquettes (pdgf)
DE4228458A1 (de) * 1992-08-27 1994-06-01 Beiersdorf Ag Multicistronische Expressionseinheiten und deren Verwendung
DE4228457A1 (de) * 1992-08-27 1994-04-28 Beiersdorf Ag Herstellung von heterodimerem PDGF-AB mit Hilfe eines bicistronischen Vektorsystems in Säugerzellen
ZA942206B (en) * 1993-04-01 1994-10-31 Amgen Inc Biologically active polypeptide fusion dimers
PT1486565E (pt) * 1995-10-11 2008-02-28 Novartis Vaccines & Diagnostic Combinação de pdgf, kgf, igf e igfbp para cicatrização de ferimentos
EP1365028B1 (fr) * 1996-12-13 2009-03-18 Novartis Vaccines and Diagnostics, Inc. Procédés permettant d'exprimer des protéines PDGF ou IGF dans la levure
WO2016061219A1 (fr) 2014-10-14 2016-04-21 Samuel Lynch Compositions pour le traitement de plaies

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH064673B2 (ja) * 1980-11-10 1994-01-19 ゲネンテツク・インコ−ポレ−テツド ハイブリド型ヒト白血球インタフエロン
US4840896A (en) * 1983-11-02 1989-06-20 Integrated Genetics, Inc. Heteropolymeric protein
AU600885B2 (en) * 1984-05-25 1990-08-30 Zymogenetics Inc. Stable DNA constructs
US4801542A (en) * 1984-10-12 1989-01-31 Zymogenetics, Inc. Expression of biologically active PDGF analogs in eucaryotic cells

Also Published As

Publication number Publication date
JPS63119682A (ja) 1988-05-24
EP0259632B1 (fr) 1995-12-13
ATE131532T1 (de) 1995-12-15
EP0259632A1 (fr) 1988-03-16
DE3751632T2 (de) 1996-06-13
AU7681687A (en) 1988-02-18
CA1341398C (fr) 2002-11-19
DE3751632D1 (de) 1996-01-25
JPH085797B2 (ja) 1996-01-24
DK421787A (da) 1988-02-14
DK421787D0 (da) 1987-08-12

Similar Documents

Publication Publication Date Title
US4849407A (en) Biologically active mosaic proteins
DK176036B1 (da) Ekspression af biologisk aktive PDGF analoger i eukaroyte celler
US4766073A (en) Expression of biologically active PDGF analogs in eucaryotic cells
US5187263A (en) Expression of biologically active PDGE analogs in eucaryotic cells
DE3588218T2 (de) Von Blutplättchen abstammende biologisch aktive Analoga eines Wachstumsfaktors in eukaryotischen Zellen
US5516896A (en) Biologically active B-chain homodimers
US4769328A (en) Expression of biologically active PDGF analogs in yeast
US5889149A (en) Purified PDGF AB isoform and method of making it
NL8900178A (nl) Groei regulerende clycoproteinen.
US5045633A (en) Expression of biologically active PDGF analogs in eucaryotic cells
US5115096A (en) Amphiregulin: a bifunctional growth modulating glycoprotein
NO175312B (no) Fremgangsmåte for rensing av et polypeptid med en del av den strukturelle oppbygning til blodplate-avledet vekstfaktor, samt monoklonalt antistoff som er spesifikt for en epitop derav
EP0547064B1 (fr) Facteur de croissance provenant des plaquettes humaines (pdgf) resistant aux proteases et procedes d'utilisation
US6004929A (en) Biologically active PDGF A-chain homodimers
US5498600A (en) Biologically active mosaic proteins
US5474982A (en) PDGF analogs and methods of use
AU641816B2 (en) Expression of biologically active PDGF analogs in eucaryotic cells
DD296700A5 (de) Verfahren zur herstellung einer fuer das amphiregulin-gen kodierenden nucleotidsequenz und von modifizierten zellen, welche diese nucleotidsequenz enthalten.
NO179453B (no) Fremgangsmåte for fremstilling av terapeutisk aktivt amphiregulin, vertscelle som produserer proteinet, rekombinant vektor inneholdt i vertscellen, samt nukleotidsekvens som koder for proteiner med amphiregulinaktivitet

Legal Events

Date Code Title Description
PUP Patent expired