DE3751632D1 - Expression von biologisch-aktiven PDGF-Analogen in eukaryotischen Zellen - Google Patents

Expression von biologisch-aktiven PDGF-Analogen in eukaryotischen Zellen

Info

Publication number
DE3751632D1
DE3751632D1 DE3751632T DE3751632T DE3751632D1 DE 3751632 D1 DE3751632 D1 DE 3751632D1 DE 3751632 T DE3751632 T DE 3751632T DE 3751632 T DE3751632 T DE 3751632T DE 3751632 D1 DE3751632 D1 DE 3751632D1
Authority
DE
Germany
Prior art keywords
pdgf
chain
chains
disclosed
biologically active
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE3751632T
Other languages
English (en)
Other versions
DE3751632T2 (de
Inventor
Mark J Murray
James D Kelly
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Zymogenetics Inc
Original Assignee
Zymogenetics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US06/896,485 external-priority patent/US4766073A/en
Priority claimed from US06/941,970 external-priority patent/US4849407A/en
Application filed by Zymogenetics Inc filed Critical Zymogenetics Inc
Application granted granted Critical
Publication of DE3751632D1 publication Critical patent/DE3751632D1/de
Publication of DE3751632T2 publication Critical patent/DE3751632T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/49—Platelet-derived growth factor [PDGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/11011—Alpharetrovirus, e.g. avian leucosis virus
    • C12N2740/11022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
DE3751632T 1986-08-13 1987-08-11 Expression von biologisch-aktiven PDGF-Analogen in eukaryotischen Zellen Expired - Lifetime DE3751632T2 (de)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US06/896,485 US4766073A (en) 1985-02-25 1986-08-13 Expression of biologically active PDGF analogs in eucaryotic cells
US06/942,161 US4845075A (en) 1985-02-25 1986-12-15 Biologically active B-chain homodimers
US06/941,970 US4849407A (en) 1986-08-13 1986-12-15 Biologically active mosaic proteins
US06/942,484 US4889919A (en) 1986-08-13 1986-12-15 Biologically active PDGF derived A-chain homodimers

Publications (2)

Publication Number Publication Date
DE3751632D1 true DE3751632D1 (de) 1996-01-25
DE3751632T2 DE3751632T2 (de) 1996-06-13

Family

ID=27505982

Family Applications (1)

Application Number Title Priority Date Filing Date
DE3751632T Expired - Lifetime DE3751632T2 (de) 1986-08-13 1987-08-11 Expression von biologisch-aktiven PDGF-Analogen in eukaryotischen Zellen

Country Status (7)

Country Link
EP (1) EP0259632B1 (de)
JP (1) JPH085797B2 (de)
AT (1) ATE131532T1 (de)
AU (1) AU7681687A (de)
CA (1) CA1341398C (de)
DE (1) DE3751632T2 (de)
DK (1) DK176036B1 (de)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU624789B2 (en) * 1987-02-19 1992-06-25 Amgen, Inc. Purified platelet-derived growth factor and methods for purification thereof
US6939849B1 (en) 1987-04-22 2005-09-06 Chiron Corporation Recombinant PDGF A-chain homodimers and methods of use
ES2091749T3 (es) * 1987-04-22 1996-11-16 Chiron Corp Produccion por recombinacion de polipeptidos de la cadena a del factor de crecimiento derivado de plaquetas (fcdp).
ATE144535T1 (de) * 1988-06-30 1996-11-15 Cor Therapeutics Inc Plättchen blockierende peptide
EP0366816A1 (de) * 1988-10-31 1990-05-09 Rorer Biotechnology Inc. Rekombinierte A-Kette von Blutplättchen Wachstumsfaktor aus Endothelzellen
DE3900770A1 (de) * 1989-01-12 1990-07-26 Juergen Prof Dr Hoppe Verfahren zur herstellung von pdgf-a und pdgf-aa
US5968778A (en) * 1989-01-12 1999-10-19 Jurgen Hoppe PDGF-AB, preparation process and pharmaceuticals containing them
IL96682A0 (en) * 1989-12-15 1991-09-16 Amgen Inc Production of biologically active platelet-derived growth factor from high expression host cell systems
US5272064A (en) * 1989-12-19 1993-12-21 Amgen Inc. DNA molecules encoding platelet-derived growth factor B chain analogs and method for expression thereof
DE69102651T2 (de) * 1990-07-23 1994-10-06 Zymogenetics Inc Proteaseresistenter pdgf und dessen verwendungen.
AU648630B2 (en) * 1990-12-21 1994-04-28 Creative Biomolecules, Inc. Biosynthetic PDGF antagonists
GB9101645D0 (en) * 1991-01-25 1991-03-06 British Bio Technology Compounds
WO1993020204A1 (en) * 1992-03-30 1993-10-14 Schering Corporation Monomeric platelet-derived growth factor and prevention of stenosis or restenosis
WO1993025576A2 (en) * 1992-06-05 1993-12-23 Sri International Peptides having platelet-derived growth factor (pdgf) activity
DE4228458A1 (de) * 1992-08-27 1994-06-01 Beiersdorf Ag Multicistronische Expressionseinheiten und deren Verwendung
DE4228457A1 (de) * 1992-08-27 1994-04-28 Beiersdorf Ag Herstellung von heterodimerem PDGF-AB mit Hilfe eines bicistronischen Vektorsystems in Säugerzellen
ZA942206B (en) * 1993-04-01 1994-10-31 Amgen Inc Biologically active polypeptide fusion dimers
EP1486565B1 (de) * 1995-10-11 2007-11-21 Novartis Vaccines and Diagnostics, Inc. Kombinierung von PDGF, KGF, IGF und IGFBP für Wundheilung
EP0948538B1 (de) * 1996-12-13 2008-06-11 Novartis Vaccines and Diagnostics, Inc. Analyse und separation von pdgf-proteinen
WO2016061219A1 (en) 2014-10-14 2016-04-21 Samuel Lynch Compositions for treating wounds

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH064673B2 (ja) * 1980-11-10 1994-01-19 ゲネンテツク・インコ−ポレ−テツド ハイブリド型ヒト白血球インタフエロン
US4840896A (en) * 1983-11-02 1989-06-20 Integrated Genetics, Inc. Heteropolymeric protein
AU600885B2 (en) * 1984-05-25 1990-08-30 Zymogenetics Inc. Stable DNA constructs
US4801542A (en) * 1984-10-12 1989-01-31 Zymogenetics, Inc. Expression of biologically active PDGF analogs in eucaryotic cells

Also Published As

Publication number Publication date
CA1341398C (en) 2002-11-19
DK421787D0 (da) 1987-08-12
JPS63119682A (ja) 1988-05-24
JPH085797B2 (ja) 1996-01-24
EP0259632A1 (de) 1988-03-16
DE3751632T2 (de) 1996-06-13
EP0259632B1 (de) 1995-12-13
DK176036B1 (da) 2006-01-09
ATE131532T1 (de) 1995-12-15
AU7681687A (en) 1988-02-18
DK421787A (da) 1988-02-14

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