DK175120B1 - Rekombinant DNA-molekyle, transformant, 2,5-DKG-reduktase, fremgangsmåde til fremstilling af en 2,5-DKG-reduktase, fremgangsmåde til fremstilling af 2-KLG ud fra glucose samt fremgangsmåde til fremstilling af vitamin C - Google Patents

Rekombinant DNA-molekyle, transformant, 2,5-DKG-reduktase, fremgangsmåde til fremstilling af en 2,5-DKG-reduktase, fremgangsmåde til fremstilling af 2-KLG ud fra glucose samt fremgangsmåde til fremstilling af vitamin C Download PDF

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Publication number
DK175120B1
DK175120B1 DK198701694A DK169487A DK175120B1 DK 175120 B1 DK175120 B1 DK 175120B1 DK 198701694 A DK198701694 A DK 198701694A DK 169487 A DK169487 A DK 169487A DK 175120 B1 DK175120 B1 DK 175120B1
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DK
Denmark
Prior art keywords
klg
dkg reductase
dkg
glucose
promoters
Prior art date
Application number
DK198701694A
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Danish (da)
English (en)
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DK169487A (da
DK169487D0 (da
Inventor
Kimber Hardy
Hendrick Van De Pol
June Grindley
Mark Payton
Original Assignee
Genencor Int
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Publication date
Priority claimed from GB858519536A external-priority patent/GB8519536D0/en
Application filed by Genencor Int filed Critical Genencor Int
Publication of DK169487A publication Critical patent/DK169487A/da
Publication of DK169487D0 publication Critical patent/DK169487D0/da
Application granted granted Critical
Publication of DK175120B1 publication Critical patent/DK175120B1/da

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01274—2,5-Didehydrogluconate reductase (1.1.1.274), i.e. 2,5-diketo-D-gluconic acid reductase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/58—Aldonic, ketoaldonic or saccharic acids
    • C12P7/60—2-Ketogulonic acid

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Vessels, Lead-In Wires, Accessory Apparatuses For Cathode-Ray Tubes (AREA)
  • Investigating Strength Of Materials By Application Of Mechanical Stress (AREA)
  • Joining Of Building Structures In Genera (AREA)
DK198701694A 1985-08-02 1987-04-02 Rekombinant DNA-molekyle, transformant, 2,5-DKG-reduktase, fremgangsmåde til fremstilling af en 2,5-DKG-reduktase, fremgangsmåde til fremstilling af 2-KLG ud fra glucose samt fremgangsmåde til fremstilling af vitamin C DK175120B1 (da)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
GB8519536 1985-08-02
GB858519536A GB8519536D0 (en) 1985-08-02 1985-08-02 Vitamin c precursor
US79243285A 1985-10-29 1985-10-29
US79243285 1985-10-29
US8601571 1986-08-01
PCT/US1986/001571 WO1987000863A1 (en) 1985-08-02 1986-08-01 Production of a vitamin c precursor using genetically modified organisms

Publications (3)

Publication Number Publication Date
DK169487A DK169487A (da) 1987-04-02
DK169487D0 DK169487D0 (da) 1987-04-02
DK175120B1 true DK175120B1 (da) 2004-06-07

Family

ID=26289601

Family Applications (1)

Application Number Title Priority Date Filing Date
DK198701694A DK175120B1 (da) 1985-08-02 1987-04-02 Rekombinant DNA-molekyle, transformant, 2,5-DKG-reduktase, fremgangsmåde til fremstilling af en 2,5-DKG-reduktase, fremgangsmåde til fremstilling af 2-KLG ud fra glucose samt fremgangsmåde til fremstilling af vitamin C

Country Status (7)

Country Link
EP (1) EP0231354B1 (ja)
JP (4) JPH0817706B2 (ja)
AT (1) ATE78297T1 (ja)
AU (1) AU594080B2 (ja)
DE (1) DE3686041T2 (ja)
DK (1) DK175120B1 (ja)
WO (1) WO1987000863A1 (ja)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5008193A (en) * 1984-06-14 1991-04-16 Genentech, Inc. Ascorbic acid intermediates and process enzymes
US5032514A (en) * 1988-08-08 1991-07-16 Genentech, Inc. Metabolic pathway engineering to increase production of ascorbic acid intermediates
US5376544A (en) * 1992-09-08 1994-12-27 Rutgers The State University Of New Jersey Enzymes for the production of 2-keto-L-gulonic acid
US5795761A (en) * 1996-01-11 1998-08-18 Rutgers, The State University Of New Jersey Mutants of 2,5-diketo-D-gluconic acid (2,5-DKG) reductase A
US6916646B1 (en) * 1997-06-23 2005-07-12 Genencor International, Inc. Enterobacteriaceae fermentation strains
US6407046B1 (en) 1998-09-03 2002-06-18 Genencor International, Inc. Mutant EGIII cellulase, DNA encoding such EGIII compositions and methods for obtaining same
US6187732B1 (en) 1998-09-03 2001-02-13 Genencor International, Inc. Mutant EGIII cellulase, DNA encoding such EGIII compositions and methods for obtaining same
US6358715B1 (en) 1998-12-04 2002-03-19 Genencor International, Inc. Production of ascorbic acid
US6576452B1 (en) 2000-10-04 2003-06-10 Genencor International, Inc. 2,5-diketo-L-gluconic acid reductases and methods of use
CN112430560B (zh) * 2019-08-26 2024-01-05 中国科学院分子植物科学卓越创新中心 一种2-酮基-l-古龙酸生产菌株及其构建方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS57186496A (en) * 1981-05-11 1982-11-16 Ajinomoto Co Inc Preparation of l-threonine by fermentation
IL72225A (en) * 1983-06-28 1990-08-31 Genentech Inc 2,5-diketogluconic acid reductase,its preparation and use in converting 2,5-diketogluconic acid into 2-keto-l-gluconic acid
JPS60118186A (ja) * 1983-11-17 1985-06-25 Shionogi & Co Ltd ２・５−ジケト−ｄ−グルコン酸リダクタ−ゼ

Also Published As

Publication number Publication date
EP0231354B1 (en) 1992-07-15
ATE78297T1 (de) 1992-08-15
DK169487A (da) 1987-04-02
EP0231354A4 (en) 1988-10-06
AU6221086A (en) 1987-03-05
JP2577876B2 (ja) 1997-02-05
JPH06319564A (ja) 1994-11-22
DK169487D0 (da) 1987-04-02
WO1987000863A1 (en) 1987-02-12
AU594080B2 (en) 1990-03-01
DE3686041D1 (de) 1992-08-20
JPH0838185A (ja) 1996-02-13
DE3686041T2 (de) 1993-01-28
JP2526021B2 (ja) 1996-08-21
JP2577877B2 (ja) 1997-02-05
JPH0817706B2 (ja) 1996-02-28
EP0231354A1 (en) 1987-08-12
JPH0838189A (ja) 1996-02-13
JPS63500493A (ja) 1988-02-25

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B1 Patent granted (law 1993)
PBP Patent lapsed
PUP Patent expired