DK173366B1 - Fremgangsmåde til isolering og rensning af hirudin fra komplekse og saltholdige opløsninger - Google Patents

Fremgangsmåde til isolering og rensning af hirudin fra komplekse og saltholdige opløsninger Download PDF

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Publication number
DK173366B1
DK173366B1 DK198806306A DK630688A DK173366B1 DK 173366 B1 DK173366 B1 DK 173366B1 DK 198806306 A DK198806306 A DK 198806306A DK 630688 A DK630688 A DK 630688A DK 173366 B1 DK173366 B1 DK 173366B1
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DK
Denmark
Prior art keywords
hirudin
complex
acetic acid
isolating
saline solutions
Prior art date
Application number
DK198806306A
Other languages
Danish (da)
English (en)
Other versions
DK630688A (da
DK630688D0 (da
Inventor
Paul Habermann
Dominique Tripier
Peter Crause
Werner Badziong
Original Assignee
Hoechst Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoechst Ag filed Critical Hoechst Ag
Publication of DK630688D0 publication Critical patent/DK630688D0/da
Publication of DK630688A publication Critical patent/DK630688A/da
Application granted granted Critical
Publication of DK173366B1 publication Critical patent/DK173366B1/da

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/815—Protease inhibitors from leeches, e.g. hirudin, eglin

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biophysics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Nitrogen And Oxygen Or Sulfur-Condensed Heterocyclic Ring Systems (AREA)
  • Extrusion Moulding Of Plastics Or The Like (AREA)
  • Communication Cables (AREA)
  • Two-Way Televisions, Distribution Of Moving Picture Or The Like (AREA)
  • Seal Device For Vehicle (AREA)
  • Orthopedics, Nursing, And Contraception (AREA)
  • Professional, Industrial, Or Sporting Protective Garments (AREA)
  • Insulated Conductors (AREA)
  • Magnetic Heads (AREA)
  • Cable Accessories (AREA)
  • Graft Or Block Polymers (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
DK198806306A 1987-11-13 1988-11-11 Fremgangsmåde til isolering og rensning af hirudin fra komplekse og saltholdige opløsninger DK173366B1 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19873738541 DE3738541A1 (de) 1987-11-13 1987-11-13 Verfahren zur isolierung und reinigung von hirudin
DE3738541 1987-11-13

Publications (3)

Publication Number Publication Date
DK630688D0 DK630688D0 (da) 1988-11-11
DK630688A DK630688A (da) 1989-05-14
DK173366B1 true DK173366B1 (da) 2000-08-14

Family

ID=6340409

Family Applications (1)

Application Number Title Priority Date Filing Date
DK198806306A DK173366B1 (da) 1987-11-13 1988-11-11 Fremgangsmåde til isolering og rensning af hirudin fra komplekse og saltholdige opløsninger

Country Status (9)

Country Link
US (1) US5095092A (pt)
EP (1) EP0316650B1 (pt)
JP (1) JP2756279B2 (pt)
AT (1) ATE100109T1 (pt)
DE (2) DE3738541A1 (pt)
DK (1) DK173366B1 (pt)
ES (1) ES2049238T3 (pt)
IE (1) IE62538B1 (pt)
PT (1) PT88975B (pt)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5268296A (en) * 1988-06-11 1993-12-07 Ciba-Geigy Corporation DNA vector and recombinant host cell for production of hirullin P6 and P18
DE3835815A1 (de) * 1988-10-21 1990-04-26 Hoechst Ag Neue isohirudine
DE4140381A1 (de) * 1991-12-07 1993-06-09 Hoechst Ag, 6230 Frankfurt, De Neue synthetische isohirudine mit verbesserter stabilitaet
DE4404168A1 (de) * 1994-02-10 1995-08-17 Hoechst Ag Hirudinderivate und Verfahren zu deren Herstellung
KR0141651B1 (ko) * 1994-09-07 1998-06-15 강재헌 금속이온 친화성 크로마토그라피를 이용한 히루딘의 정제방법
US7638618B2 (en) 2001-02-20 2009-12-29 Sanofi-Aventis Deutschland Gmbh Nucleic acids encoding a hirudin and pro-insulin as superscretable peptides and for parallel improvement of the exported forms of one or more polypeptides of interest
DE10108211A1 (de) * 2001-02-20 2002-08-22 Aventis Pharma Gmbh Verwendung von Fusionsproteinen, deren N-terminaler Anteil aus einem Hirudinderivat besteht, zur Herstellung rekombinanter Proteine über Sekretion durch Hefen
WO2003059935A2 (en) * 2001-12-21 2003-07-24 Immunex Corporation Methods for purifying protein
US7795205B2 (en) 2004-04-12 2010-09-14 Canyon Pharmaceuticals, Inc. Methods for effecting regression of tumor mass and size in a metastasized pancreatic tumor
CN101005859B (zh) * 2004-06-29 2012-08-15 利莱恩斯生命科学有限公司 灭病毒生物液体的制备方法
JP6845139B2 (ja) * 2014-12-15 2021-03-17 メルク パテント ゲゼルシャフト ミット ベシュレンクテル ハフツングＭｅｒｃｋ Ｐａｔｅｎｔ Ｇｅｓｅｌｌｓｃｈａｆｔ ｍｉｔ ｂｅｓｃｈｒａｅｎｋｔｅｒ Ｈａｆｔｕｎｇ 粗製溶液からの標的分子捕捉
CN107353338B (zh) * 2017-07-27 2020-11-17 宁波博睿修存生物科技有限公司 一种分离水蛭素发酵液中色素分子的方法
CN113984936A (zh) * 2021-12-09 2022-01-28 车延柠 一种水蛭酶解多肽的lc-ms特征图谱、检测方法及其应用

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES8207217A1 (es) * 1980-10-09 1982-09-01 Hoechst Ag Procedimiento para la preparacion de un inactivador de alfa-amilasa
DE3342139A1 (de) * 1983-11-22 1985-05-30 Ciba-Geigy Ag, Basel Desulfatohirudine, verfahren zu ihrer herstellung und pharmazeutische mittel
DE3438296A1 (de) * 1984-04-18 1985-11-07 Hoechst Ag, 6230 Frankfurt Neue polypeptide mit blutgerinnungshemmender wirkung, verfahren zu deren herstellung bzw. gewinnung, deren verwendung und diese enthaltende mittel
DE3429430A1 (de) * 1984-08-10 1986-02-20 Hoechst Ag, 6230 Frankfurt Gentechnologisches verfahren zur herstellung von hirudin und mittel zur durchfuehrung dieses verfahrens
US4616078A (en) * 1985-04-08 1986-10-07 Eli Lilly And Company Process for purifying proinsulin-like materials
FR2593518B1 (fr) * 1985-05-02 1989-09-08 Transgene Sa Vecteurs d'expression et de secretion de l'hirudine par les levures transformees
US4617376A (en) * 1985-07-01 1986-10-14 Eli Lilly And Company Process for recovering glucagon from pancreas glands
DE3689525D1 (de) * 1985-07-17 1994-02-24 Hoechst Ag Neue Polypeptide mit blutgerinnungshemmender Wirkung, Verfahren zu deren Herstellung bzw. Gewinnung, deren Verwendung und diese enthaltende Mittel.
DE3526995A1 (de) * 1985-07-27 1987-02-05 Hoechst Ag Fusionsproteine, verfahren zu ihrer herstellung und ihre verwendung

Also Published As

Publication number Publication date
JP2756279B2 (ja) 1998-05-25
DK630688A (da) 1989-05-14
EP0316650B1 (de) 1994-01-12
IE883393L (en) 1989-05-13
ES2049238T3 (es) 1994-04-16
IE62538B1 (en) 1995-02-08
PT88975A (pt) 1989-11-30
JPH01165599A (ja) 1989-06-29
DK630688D0 (da) 1988-11-11
ATE100109T1 (de) 1994-01-15
DE3887095D1 (de) 1994-02-24
DE3738541A1 (de) 1989-05-24
EP0316650A3 (en) 1990-07-25
EP0316650A2 (de) 1989-05-24
US5095092A (en) 1992-03-10
PT88975B (pt) 1994-11-30

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Date Code Title Description
B1 Patent granted (law 1993)
PUP Patent expired