DK1292613T3 - Kimært GPF-Aequorin som bioluminiscerende Ca-reportere på enkeltcelleniveau - Google Patents

Kimært GPF-Aequorin som bioluminiscerende Ca-reportere på enkeltcelleniveau

Info

Publication number
DK1292613T3
DK1292613T3 DK01949426T DK01949426T DK1292613T3 DK 1292613 T3 DK1292613 T3 DK 1292613T3 DK 01949426 T DK01949426 T DK 01949426T DK 01949426 T DK01949426 T DK 01949426T DK 1292613 T3 DK1292613 T3 DK 1292613T3
Authority
DK
Denmark
Prior art keywords
gpf
aequorin
reporters
chimeric
cell bioluminescent
Prior art date
Application number
DK01949426T
Other languages
Danish (da)
English (en)
Inventor
Philippe Brulet
Valerie Baubet
Mouellic Herve Le
Original Assignee
Pasteur Institut
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pasteur Institut filed Critical Pasteur Institut
Application granted granted Critical
Publication of DK1292613T3 publication Critical patent/DK1292613T3/da

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/04—Linear peptides containing only normal peptide links
    • C07K7/06—Linear peptides containing only normal peptide links having 5 to 11 amino acids
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K49/00—Preparations for testing in vivo
    • A61K49/001—Preparation for luminescence or biological staining
    • A61K49/0013—Luminescence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/001—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof by chemical synthesis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43595—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from coelenteratae, e.g. medusae
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/04—Linear peptides containing only normal peptide links
    • C07K7/08—Linear peptides containing only normal peptide links having 12 to 20 amino acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/968—High energy substrates, e.g. fluorescent, chemiluminescent, radioactive
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S930/00—Peptide or protein sequence
    • Y10S930/01—Peptide or protein sequence
    • Y10S930/31—Linker sequence

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Public Health (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
DK01949426T 2000-06-01 2001-06-01 Kimært GPF-Aequorin som bioluminiscerende Ca-reportere på enkeltcelleniveau DK1292613T3 (da)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US20831400P 2000-06-01 2000-06-01
US21052600P 2000-06-09 2000-06-09
US25511100P 2000-12-14 2000-12-14
PCT/EP2001/007057 WO2001092300A2 (en) 2000-06-01 2001-06-01 Chimeric gfp-aequorin as bioluminescent ca++ reporters at the single cell level

Publications (1)

Publication Number Publication Date
DK1292613T3 true DK1292613T3 (da) 2008-11-24

Family

ID=27395180

Family Applications (1)

Application Number Title Priority Date Filing Date
DK01949426T DK1292613T3 (da) 2000-06-01 2001-06-01 Kimært GPF-Aequorin som bioluminiscerende Ca-reportere på enkeltcelleniveau

Country Status (14)

Country Link
US (3) US6936475B2 (de)
EP (1) EP1292613B9 (de)
JP (1) JP5389307B2 (de)
CN (1) CN100467604C (de)
AT (1) ATE407142T1 (de)
AU (1) AU784887B2 (de)
CA (1) CA2411304C (de)
CY (1) CY1110438T1 (de)
DE (1) DE60135652D1 (de)
DK (1) DK1292613T3 (de)
ES (1) ES2312454T3 (de)
HK (1) HK1060370A1 (de)
PT (1) PT1292613E (de)
WO (1) WO2001092300A2 (de)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PT1292613E (pt) * 2000-06-01 2008-11-10 Pasteur Institut Gpf-aequorina quiméria como repórteres de ca++ bioluminiscentes, ao nível da célula singular
CA2421858A1 (en) * 2000-09-07 2002-03-14 Bjorn A. Olde Reporter system for cell surface receptor-ligand binding
DE03779067T1 (de) * 2002-11-12 2006-06-22 Zakrytoe Aktsionernoe Obschestvo "Evrogen" Fluoreszenzproteine und chromoproteine aus nicht-aequorea-hydrozoa-spezies sowie verfahren zur verwendung davon
CN1304571C (zh) * 2003-10-16 2007-03-14 清华大学 一种培育转基因斑马鱼的方法及其专用质粒
EP1714155A1 (de) * 2004-02-12 2006-10-25 Institut Pasteur Nichtinvasive in-vivo-echtzeit-biolumineszenzabbildung lokaler ca2+-dynamik in lebenden organismen
GB0625678D0 (en) 2006-12-21 2007-01-31 Lux Biotechnology Ltd Composition and method for detection of demineralisation
ES2427854B1 (es) * 2012-03-29 2014-09-15 Universidad De Valladolid Sensores de calcio y métodos para la detección de calcio libre intracelular
ES2452485B1 (es) * 2012-07-13 2015-03-09 Univ Valladolid Mutantes de apoacuorina y metodos para su uso
ES2445018B1 (es) * 2012-07-27 2014-12-12 Universidad De Castilla La Mancha VARIANTES DE LA PROTEÍNA DE FUSIÓN SENSIBLE AL CALCIO tdTOMATO-AEQUORINA
FR2996225B1 (fr) 2012-10-03 2014-10-03 Centre Nat Rech Scient Analogues de la coelenterazine emettant une lumiere decalee vers le rouge visant l'imagerie in vivo
GB201700317D0 (en) 2017-01-09 2017-02-22 Calcivis Ltd Detection device
WO2019241318A1 (en) * 2018-06-13 2019-12-19 Cornell University Bioluminescent markers of neural activity
USD1042180S1 (en) 2020-05-28 2024-09-17 Calcivis Limited Dental detection device

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8916806D0 (en) * 1989-07-22 1989-09-06 Univ Wales Medicine Modified proteins
US5498521A (en) * 1990-01-24 1996-03-12 President And Fellows Of Harvard College Diagnosis of hereditary retinal degenerative diseases
US5614184A (en) * 1992-07-28 1997-03-25 New England Deaconess Hospital Recombinant human erythropoietin mutants and therapeutic methods employing them
US5714666A (en) * 1993-02-09 1998-02-03 Children's Hospital Of Philadelphia Measurement of intracellular calcium using bioluminescent apoaequorin expressed in mammalian cells
US6818439B1 (en) * 1994-12-30 2004-11-16 Chiron Corporation Methods for administration of recombinant gene delivery vehicles for treatment of hemophilia and other disorders
US5744326A (en) * 1996-03-11 1998-04-28 The Immune Response Corporation Use of viral CIS-acting post-transcriptional regulatory sequences to increase expression of intronless genes containing near-consensus splice sites
AU765703B2 (en) * 1998-03-27 2003-09-25 Bruce J. Bryan Luciferases, fluorescent proteins, nucleic acids encoding the luciferases and fluorescent proteins and the use thereof in diagnostics, high throughput screening and novelty items
DK1071809T3 (da) * 1998-04-17 2003-12-01 Rigel Pharmaceuticals Inc Multiparameter FACS test til påvisning af ændringer i cellulære parametre
US6800492B2 (en) 2000-06-01 2004-10-05 Institute Pasteur Chimeric GFP-aequorin as bioluminescent Ca++ reporters at the single cell level
PT1292613E (pt) 2000-06-01 2008-11-10 Pasteur Institut Gpf-aequorina quiméria como repórteres de ca++ bioluminiscentes, ao nível da célula singular

Also Published As

Publication number Publication date
US20030175807A1 (en) 2003-09-18
US20100330667A1 (en) 2010-12-30
AU7058301A (en) 2001-12-11
CN1468312A (zh) 2004-01-14
PT1292613E (pt) 2008-11-10
JP5389307B2 (ja) 2014-01-15
WO2001092300A2 (en) 2001-12-06
DE60135652D1 (de) 2008-10-16
US6936475B2 (en) 2005-08-30
US20080213879A1 (en) 2008-09-04
CN100467604C (zh) 2009-03-11
ATE407142T1 (de) 2008-09-15
US7763443B2 (en) 2010-07-27
ES2312454T3 (es) 2009-03-01
CA2411304C (en) 2014-07-29
CA2411304A1 (en) 2001-12-06
AU784887B2 (en) 2006-07-20
CY1110438T1 (el) 2015-04-29
EP1292613A2 (de) 2003-03-19
WO2001092300A3 (en) 2002-11-07
EP1292613B1 (de) 2008-09-03
JP2004532001A (ja) 2004-10-21
EP1292613B9 (de) 2009-04-01
HK1060370A1 (en) 2004-08-06
WO2001092300A9 (en) 2009-01-22

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