CY1110438T1 - Χιμαιρικες (πρωτεϊνες συντηξης) gfp-ακουορινης ως βιοφωταυγεις αναφορεις ca++ στο επιπεδο του ενος κυτταρου - Google Patents
Χιμαιρικες (πρωτεϊνες συντηξης) gfp-ακουορινης ως βιοφωταυγεις αναφορεις ca++ στο επιπεδο του ενος κυτταρουInfo
- Publication number
- CY1110438T1 CY1110438T1 CY20081101381T CY081101381T CY1110438T1 CY 1110438 T1 CY1110438 T1 CY 1110438T1 CY 20081101381 T CY20081101381 T CY 20081101381T CY 081101381 T CY081101381 T CY 081101381T CY 1110438 T1 CY1110438 T1 CY 1110438T1
- Authority
- CY
- Cyprus
- Prior art keywords
- bioslighters
- aquorine
- gfp
- references
- chemical
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
- C07K7/04—Linear peptides containing only normal peptide links
- C07K7/06—Linear peptides containing only normal peptide links having 5 to 11 amino acids
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K49/00—Preparations for testing in vivo
- A61K49/001—Preparation for luminescence or biological staining
- A61K49/0013—Luminescence
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/001—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof by chemical synthesis
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
- C07K14/43595—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from coelenteratae, e.g. medusae
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
- C07K7/04—Linear peptides containing only normal peptide links
- C07K7/08—Linear peptides containing only normal peptide links having 12 to 20 amino acids
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/968—High energy substrates, e.g. fluorescent, chemiluminescent, radioactive
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S930/00—Peptide or protein sequence
- Y10S930/01—Peptide or protein sequence
- Y10S930/31—Linker sequence
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Gastroenterology & Hepatology (AREA)
- Engineering & Computer Science (AREA)
- Toxicology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biomedical Technology (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Tropical Medicine & Parasitology (AREA)
- General Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Ένα τροποποιημένο βιοφωταυγές σύστημα που περιέχει ένα φθορίζον μόριο (που είναι) συνδεμένο ομοιοπολικά με μία φωτοπρωτεΐνη, όπου η εν λόγω σύνδεση μεταξύ των δύο πρωτεϊνών έχει τη λειτουργία να σταθεροποιεί το τροποποιημένο βιοφωταυγές σύστημα και επιτρέποντας τη μεταφορά της ενέργειας μέσω Μεταφοράς Ενέργειας Συντονισμού Χημειοφωταύγειας (CRET).
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US20831400P | 2000-06-01 | 2000-06-01 | |
US21052600P | 2000-06-09 | 2000-06-09 | |
US25511100P | 2000-12-14 | 2000-12-14 | |
EP01949426A EP1292613B9 (en) | 2000-06-01 | 2001-06-01 | Chimeric GPF-Aequorin as bioluminiscent Ca++ reporters at the single cell level |
Publications (1)
Publication Number | Publication Date |
---|---|
CY1110438T1 true CY1110438T1 (el) | 2015-04-29 |
Family
ID=27395180
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CY20081101381T CY1110438T1 (el) | 2000-06-01 | 2008-12-02 | Χιμαιρικες (πρωτεϊνες συντηξης) gfp-ακουορινης ως βιοφωταυγεις αναφορεις ca++ στο επιπεδο του ενος κυτταρου |
Country Status (14)
Country | Link |
---|---|
US (3) | US6936475B2 (el) |
EP (1) | EP1292613B9 (el) |
JP (1) | JP5389307B2 (el) |
CN (1) | CN100467604C (el) |
AT (1) | ATE407142T1 (el) |
AU (1) | AU784887B2 (el) |
CA (1) | CA2411304C (el) |
CY (1) | CY1110438T1 (el) |
DE (1) | DE60135652D1 (el) |
DK (1) | DK1292613T3 (el) |
ES (1) | ES2312454T3 (el) |
HK (1) | HK1060370A1 (el) |
PT (1) | PT1292613E (el) |
WO (1) | WO2001092300A2 (el) |
Families Citing this family (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
ATE407142T1 (de) * | 2000-06-01 | 2008-09-15 | Pasteur Institut | Chimäres gpf-aequorin als bioluminiszenter ca++ marker auf einzelzellniveau |
WO2002020749A2 (en) * | 2000-09-07 | 2002-03-14 | Owman Christer S O | Chimeric reporter system for cell surface receptor-ligand binding |
EP1565559B1 (en) * | 2002-11-12 | 2011-05-18 | Zakrytoe Aktsionernoe Obschestvo " Evrogen" | Fluorescent proteins and chromoproteins from non-aequorea hydrozoa species and methods for using same |
CN1304571C (zh) * | 2003-10-16 | 2007-03-14 | 清华大学 | 一种培育转基因斑马鱼的方法及其专用质粒 |
EP1714155A1 (en) * | 2004-02-12 | 2006-10-25 | Institut Pasteur | Non-invasive real-time in vivo bioluminescence imaging of local ca2+ dynamics in living organisms |
GB0625678D0 (en) | 2006-12-21 | 2007-01-31 | Lux Biotechnology Ltd | Composition and method for detection of demineralisation |
ES2427854B1 (es) * | 2012-03-29 | 2014-09-15 | Universidad De Valladolid | Sensores de calcio y métodos para la detección de calcio libre intracelular |
ES2452485B1 (es) * | 2012-07-13 | 2015-03-09 | Univ Valladolid | Mutantes de apoacuorina y metodos para su uso |
ES2445018B1 (es) * | 2012-07-27 | 2014-12-12 | Universidad De Castilla La Mancha | VARIANTES DE LA PROTEÍNA DE FUSIÓN SENSIBLE AL CALCIO tdTOMATO-AEQUORINA |
FR2996225B1 (fr) | 2012-10-03 | 2014-10-03 | Centre Nat Rech Scient | Analogues de la coelenterazine emettant une lumiere decalee vers le rouge visant l'imagerie in vivo |
GB201700317D0 (en) | 2017-01-09 | 2017-02-22 | Calcivis Ltd | Detection device |
WO2019241318A1 (en) * | 2018-06-13 | 2019-12-19 | Cornell University | Bioluminescent markers of neural activity |
Family Cites Families (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB8916806D0 (en) * | 1989-07-22 | 1989-09-06 | Univ Wales Medicine | Modified proteins |
US5498521A (en) * | 1990-01-24 | 1996-03-12 | President And Fellows Of Harvard College | Diagnosis of hereditary retinal degenerative diseases |
US5614184A (en) * | 1992-07-28 | 1997-03-25 | New England Deaconess Hospital | Recombinant human erythropoietin mutants and therapeutic methods employing them |
US5714666A (en) * | 1993-02-09 | 1998-02-03 | Children's Hospital Of Philadelphia | Measurement of intracellular calcium using bioluminescent apoaequorin expressed in mammalian cells |
US6818439B1 (en) * | 1994-12-30 | 2004-11-16 | Chiron Corporation | Methods for administration of recombinant gene delivery vehicles for treatment of hemophilia and other disorders |
US5744326A (en) * | 1996-03-11 | 1998-04-28 | The Immune Response Corporation | Use of viral CIS-acting post-transcriptional regulatory sequences to increase expression of intronless genes containing near-consensus splice sites |
CA2324648C (en) * | 1998-03-27 | 2013-02-26 | Prolume, Ltd. | Luciferases, fluorescent proteins, nucleic acids encoding the luciferases and fluorescent proteins and the use thereof in diagnostics, high throughput screening and novelty items |
CA2325597C (en) * | 1998-04-17 | 2010-09-21 | Rigel Pharmaceuticals, Inc. | Multiparameter facs assays to detect alterations in cellular parameters and to screen small molecule libraries |
ATE407142T1 (de) * | 2000-06-01 | 2008-09-15 | Pasteur Institut | Chimäres gpf-aequorin als bioluminiszenter ca++ marker auf einzelzellniveau |
US6800492B2 (en) | 2000-06-01 | 2004-10-05 | Institute Pasteur | Chimeric GFP-aequorin as bioluminescent Ca++ reporters at the single cell level |
-
2001
- 2001-06-01 AT AT01949426T patent/ATE407142T1/de active
- 2001-06-01 PT PT01949426T patent/PT1292613E/pt unknown
- 2001-06-01 CN CN01812104.7A patent/CN100467604C/zh not_active Expired - Fee Related
- 2001-06-01 AU AU70583/01A patent/AU784887B2/en not_active Ceased
- 2001-06-01 CA CA2411304A patent/CA2411304C/en not_active Expired - Fee Related
- 2001-06-01 DE DE60135652T patent/DE60135652D1/de not_active Expired - Lifetime
- 2001-06-01 ES ES01949426T patent/ES2312454T3/es not_active Expired - Lifetime
- 2001-06-01 JP JP2002500911A patent/JP5389307B2/ja not_active Expired - Fee Related
- 2001-06-01 DK DK01949426T patent/DK1292613T3/da active
- 2001-06-01 EP EP01949426A patent/EP1292613B9/en not_active Expired - Lifetime
- 2001-06-01 WO PCT/EP2001/007057 patent/WO2001092300A2/en active IP Right Grant
-
2002
- 2002-12-02 US US10/307,389 patent/US6936475B2/en not_active Expired - Fee Related
-
2004
- 2004-05-14 HK HK04103420.0A patent/HK1060370A1/xx not_active IP Right Cessation
-
2005
- 2005-06-10 US US11/149,177 patent/US7763443B2/en not_active Expired - Fee Related
-
2008
- 2008-12-02 CY CY20081101381T patent/CY1110438T1/el unknown
-
2010
- 2010-06-04 US US12/794,147 patent/US20100330667A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
US7763443B2 (en) | 2010-07-27 |
US20080213879A1 (en) | 2008-09-04 |
DE60135652D1 (de) | 2008-10-16 |
DK1292613T3 (da) | 2008-11-24 |
ATE407142T1 (de) | 2008-09-15 |
CA2411304C (en) | 2014-07-29 |
WO2001092300A9 (en) | 2009-01-22 |
HK1060370A1 (en) | 2004-08-06 |
PT1292613E (pt) | 2008-11-10 |
EP1292613B9 (en) | 2009-04-01 |
CA2411304A1 (en) | 2001-12-06 |
CN1468312A (zh) | 2004-01-14 |
EP1292613A2 (en) | 2003-03-19 |
AU784887B2 (en) | 2006-07-20 |
AU7058301A (en) | 2001-12-11 |
WO2001092300A2 (en) | 2001-12-06 |
EP1292613B1 (en) | 2008-09-03 |
ES2312454T3 (es) | 2009-03-01 |
JP5389307B2 (ja) | 2014-01-15 |
WO2001092300A3 (en) | 2002-11-07 |
US6936475B2 (en) | 2005-08-30 |
JP2004532001A (ja) | 2004-10-21 |
CN100467604C (zh) | 2009-03-11 |
US20030175807A1 (en) | 2003-09-18 |
US20100330667A1 (en) | 2010-12-30 |
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