ES2452485B1 - Mutantes de apoacuorina y metodos para su uso - Google Patents

Mutantes de apoacuorina y metodos para su uso Download PDF

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Publication number
ES2452485B1
ES2452485B1 ES201231104A ES201231104A ES2452485B1 ES 2452485 B1 ES2452485 B1 ES 2452485B1 ES 201231104 A ES201231104 A ES 201231104A ES 201231104 A ES201231104 A ES 201231104A ES 2452485 B1 ES2452485 B1 ES 2452485B1
Authority
ES
Spain
Prior art keywords
apoacuorine
mutants
methods
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
ES201231104A
Other languages
English (en)
Other versions
ES2452485A1 (es
Inventor
ALONSO Mª TERESA ALONSO
Martin Javier Garcia-Sancho
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Universidad de Valladolid
Original Assignee
Universidad de Valladolid
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Universidad de Valladolid filed Critical Universidad de Valladolid
Priority to ES201231104A priority Critical patent/ES2452485B1/es
Priority to PCT/ES2013/070503 priority patent/WO2014009590A1/es
Publication of ES2452485A1 publication Critical patent/ES2452485A1/es
Application granted granted Critical
Publication of ES2452485B1 publication Critical patent/ES2452485B1/es
Active legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/84—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving inorganic compounds or pH
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y113/00—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
    • C12Y113/12—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of one atom of oxygen (internal monooxygenases or internal mixed function oxidases)(1.13.12)
    • C12Y113/12005—Renilla-luciferin 2-monooxygenase (1.13.12.5), i.e. renilla-luciferase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/04—Fusion polypeptide containing a localisation/targetting motif containing an ER retention signal such as a C-terminal HDEL motif
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/60—Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Inorganic Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
ES201231104A 2012-07-13 2012-07-13 Mutantes de apoacuorina y metodos para su uso Active ES2452485B1 (es)

Priority Applications (2)

Application Number Priority Date Filing Date Title
ES201231104A ES2452485B1 (es) 2012-07-13 2012-07-13 Mutantes de apoacuorina y metodos para su uso
PCT/ES2013/070503 WO2014009590A1 (es) 2012-07-13 2013-07-12 Mutantes de apoacuorina y metodos para su uso

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES201231104A ES2452485B1 (es) 2012-07-13 2012-07-13 Mutantes de apoacuorina y metodos para su uso

Publications (2)

Publication Number Publication Date
ES2452485A1 ES2452485A1 (es) 2014-04-01
ES2452485B1 true ES2452485B1 (es) 2015-03-09

Family

ID=49915453

Family Applications (1)

Application Number Title Priority Date Filing Date
ES201231104A Active ES2452485B1 (es) 2012-07-13 2012-07-13 Mutantes de apoacuorina y metodos para su uso

Country Status (2)

Country Link
ES (1) ES2452485B1 (es)
WO (1) WO2014009590A1 (es)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MA55153A (fr) * 2016-02-19 2021-09-29 Immatics Biotechnologies Gmbh Nouveaux peptides et combinaison de peptides pour une utilisation dans l'immunothérapie contre le lymphome non hodgkinien et d'autres cancers

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5389307B2 (ja) * 2000-06-01 2014-01-15 インスティチュート・パスツール 単細胞レベルでの生物発光性Ｃａ＋＋リポーターとしてのキメラ性ＧＦＰ−エクオリン
WO2005078445A1 (en) * 2004-02-12 2005-08-25 Institut Pasteur Non-invasive real-time in vivo bioluminescence imaging of local ca2+ dynamics in living organisms

Also Published As

Publication number Publication date
ES2452485A1 (es) 2014-04-01
WO2014009590A1 (es) 2014-01-16

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