DK1035208T3 - LoxP-sekvensvarianter og deres anvendelse - Google Patents
LoxP-sekvensvarianter og deres anvendelseInfo
- Publication number
- DK1035208T3 DK1035208T3 DK98953027T DK98953027T DK1035208T3 DK 1035208 T3 DK1035208 T3 DK 1035208T3 DK 98953027 T DK98953027 T DK 98953027T DK 98953027 T DK98953027 T DK 98953027T DK 1035208 T3 DK1035208 T3 DK 1035208T3
- Authority
- DK
- Denmark
- Prior art keywords
- bases
- loxp site
- wild
- image
- specific recombination
- Prior art date
Links
- 101710200251 Recombinase cre Proteins 0.000 abstract 2
- 108090000623 proteins and genes Proteins 0.000 abstract 2
- 230000006798 recombination Effects 0.000 abstract 2
- 238000005215 recombination Methods 0.000 abstract 2
- 125000006850 spacer group Chemical group 0.000 abstract 2
- 241000588724 Escherichia coli Species 0.000 abstract 1
- 241000206602 Eukaryota Species 0.000 abstract 1
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 1
- 210000004102 animal cell Anatomy 0.000 abstract 1
- 230000010354 integration Effects 0.000 abstract 1
- 239000002773 nucleotide Substances 0.000 abstract 1
- 125000003729 nucleotide group Chemical group 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/86—Viral vectors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2217/00—Genetically modified animals
- A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2217/00—Genetically modified animals
- A01K2217/07—Animals genetically altered by homologous recombination
- A01K2217/075—Animals genetically altered by homologous recombination inducing loss of function, i.e. knock out
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/10011—Adenoviridae
- C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
- C12N2710/10341—Use of virus, viral particle or viral elements as a vector
- C12N2710/10343—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Mycology (AREA)
- Virology (AREA)
- Cell Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Detergent Compositions (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Peptides Or Proteins (AREA)
- Enzymes And Modification Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP33128997 | 1997-11-13 | ||
| JP27315098A JP4206154B2 (ja) | 1997-11-13 | 1998-09-28 | 変異型ｌｏｘＰ配列とその応用 |
| PCT/JP1998/005094 WO1999025851A1 (fr) | 1997-11-13 | 1998-11-12 | Sequences 1oxp de variant et leur application |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK1035208T3 true DK1035208T3 (da) | 2009-02-23 |
Family
ID=26550537
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK98953027T DK1035208T3 (da) | 1997-11-13 | 1998-11-12 | LoxP-sekvensvarianter og deres anvendelse |
Country Status (12)
| Country | Link |
|---|---|
| US (1) | US6465254B1 (ko) |
| EP (1) | EP1035208B1 (ko) |
| JP (1) | JP4206154B2 (ko) |
| KR (1) | KR100639088B1 (ko) |
| CN (1) | CN1173039C (ko) |
| AT (1) | ATE412759T1 (ko) |
| AU (1) | AU741415B2 (ko) |
| CA (1) | CA2309386C (ko) |
| DE (1) | DE69840173D1 (ko) |
| DK (1) | DK1035208T3 (ko) |
| NZ (1) | NZ504307A (ko) |
| WO (1) | WO1999025851A1 (ko) |
Families Citing this family (52)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6964861B1 (en) | 1998-11-13 | 2005-11-15 | Invitrogen Corporation | Enhanced in vitro recombinational cloning of using ribosomal proteins |
| US6720140B1 (en) | 1995-06-07 | 2004-04-13 | Invitrogen Corporation | Recombinational cloning using engineered recombination sites |
| US5888732A (en) | 1995-06-07 | 1999-03-30 | Life Technologies, Inc. | Recombinational cloning using engineered recombination sites |
| US5851808A (en) | 1997-02-28 | 1998-12-22 | Baylor College Of Medicine | Rapid subcloning using site-specific recombination |
| CN100342008C (zh) | 1997-10-24 | 2007-10-10 | 茵维特罗根公司 | 利用具重组位点的核酸进行重组克隆 |
| JP4153607B2 (ja) * | 1998-11-18 | 2008-09-24 | 大日本住友製薬株式会社 | 新規な組換えアデノウイルス |
| JP4580106B2 (ja) | 1999-03-02 | 2010-11-10 | ライフ テクノロジーズ コーポレーション | 核酸の組換えクローニングにおける使用のための組成物および方法 |
| WO2001011058A1 (en) * | 1999-08-09 | 2001-02-15 | Monsanto Technology Llc | Novel cloning methods and vectors |
| JP2001321174A (ja) * | 2000-05-11 | 2001-11-20 | Japan Science & Technology Corp | アンチセンス型ジーントラップベクター |
| US7198924B2 (en) | 2000-12-11 | 2007-04-03 | Invitrogen Corporation | Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites |
| FR2814642B1 (fr) | 2000-10-03 | 2005-07-01 | Ass Pour Le Dev De La Rech En | Souris transgenique pour la recombinaison ciblee mediee par la cre-er modifiee |
| EP1322152A2 (en) * | 2000-10-03 | 2003-07-02 | ADEREGEM (Association pour le Développement de la Recherche en Génétique Moléculaire | Transgenic mouse for targeted recombination mediated by modified cre-er |
| WO2002083889A2 (en) * | 2001-04-10 | 2002-10-24 | Imperial College Innovations Limited | Methods for the production of minicircles |
| US6838285B2 (en) * | 2001-09-18 | 2005-01-04 | Becton Dickinson | Site specific recombinase based method for producing adenoviral vectors |
| JP4171844B2 (ja) | 2001-11-22 | 2008-10-29 | 学校法人慶應義塾 | 超レパートリー人工抗体ライブラリー |
| US6818420B2 (en) | 2002-02-27 | 2004-11-16 | Biosource International, Inc. | Methods of using FET labeled oligonucleotides that include a 3′-5′ exonuclease resistant quencher domain and compositions for practicing the same |
| AU2003231244A1 (en) | 2002-05-02 | 2003-11-17 | University Of North Carolina At Chapel Hill | In vitro mutagenesis, phenotyping, and gene mapping |
| EP1572943B1 (en) | 2002-08-29 | 2015-04-22 | The Board of Trustees of The Leland S. Stanford Junior University | Circular nucleic acid vectors, and methods for making and using the same |
| US20040216178A1 (en) * | 2003-03-03 | 2004-10-28 | Jones Stephen N | Regulation of mdm2 function |
| EP2202322A1 (en) | 2003-10-31 | 2010-06-30 | AB Advanced Genetic Analysis Corporation | Methods for producing a paired tag from a nucleic acid sequence and methods of use thereof |
| EP1697534B1 (en) | 2003-12-01 | 2010-06-02 | Life Technologies Corporation | Nucleic acid molecules containing recombination sites and methods of using the same |
| WO2005081632A2 (en) * | 2004-02-26 | 2005-09-09 | State Of Israel, Ministry Of Agriculture, Agricultural Research Organization | Enzymes, cells and methods for site specific recombination at asymmetric sites |
| JP2006333742A (ja) * | 2005-05-31 | 2006-12-14 | Chemicals Evaluation & Research Institute | 細胞及び細胞へのｄｎａ導入方法 |
| ES2390132T3 (es) | 2005-07-18 | 2012-11-06 | Pioneer Hi-Bred International Inc. | Sitios de recombinación FRT modificados y métodos de uso |
| EP1948803A4 (en) * | 2005-10-13 | 2009-11-25 | Bc Cancer Agency | MODULAR GENOME FOR SYNTHETIC BIOLOGY AND METABOLISM MANIPULATION |
| US20070143881A1 (en) * | 2005-12-16 | 2007-06-21 | Pioneer Hi-Bred International, Inc. | Methods and Compositions for Improving the Efficiency of Site-Specific Polynucleotide Exchange |
| CA2651895C (en) | 2006-05-09 | 2014-05-06 | The Curators Of The University Of Missouri | Plant artificial chromosome platforms via telomere truncation |
| CN101490267B (zh) | 2006-05-17 | 2013-04-17 | 先锋高级育种国际公司 | 人工植物微染色体 |
| EP2167666A2 (en) | 2007-06-29 | 2010-03-31 | Pioneer Hi-Bred International Inc. | Methods for altering the genome of a monocot plant cell |
| EP2067858A1 (en) | 2007-12-07 | 2009-06-10 | Universidad de Sevilla | Animal models for neurodegenerative diseases |
| US8530197B2 (en) | 2008-01-09 | 2013-09-10 | Applied Biosystems, Llc | Method of making a paired tag library for nucleic acid sequencing |
| WO2012044847A1 (en) | 2010-10-01 | 2012-04-05 | Life Technologies Corporation | Nucleic acid adaptors and uses thereof |
| ES2337973B8 (es) | 2008-05-16 | 2011-07-21 | Proyecto De Biomedicina Cima, S.L. | Adenovirus auxiliares auto-inactivantes para la produccion de adenovirus recombinantes de alta capacidad. |
| CN102939383B (zh) | 2009-12-30 | 2015-04-29 | 先锋国际良种公司 | 用于靶向多核苷酸修饰的方法和组合物 |
| US10030245B2 (en) | 2011-03-23 | 2018-07-24 | E I Du Pont De Nemours And Company | Methods for producing a complex transgenic trait locus |
| SG194089A1 (en) | 2011-04-27 | 2013-11-29 | Amyris Inc | Methods for genomic modification |
| WO2013066423A2 (en) | 2011-06-21 | 2013-05-10 | Pioneer Hi-Bred International, Inc. | Methods and compositions for producing male sterile plants |
| US9657309B2 (en) * | 2012-03-12 | 2017-05-23 | Dsm Ip Assets B.V. | Recombination system |
| CN103361342B (zh) * | 2012-03-30 | 2019-01-25 | 上海杰隆生物工程股份有限公司 | 一种转基因定位整合的方法及其应用 |
| US20140173781A1 (en) | 2012-12-13 | 2014-06-19 | Pioneer Hi-Bred International, Inc. | Methods and compositions for producing and selecting transgenic wheat plants |
| ES2523016B1 (es) | 2013-05-20 | 2015-09-09 | 3P Biopharmaceuticals | Vectores alfavirales y líneas celulares para la producción de proteínas recombinantes |
| EP3083958B1 (en) | 2013-12-19 | 2019-04-17 | Amyris, Inc. | Methods for genomic integration |
| MX2017006127A (es) | 2014-11-11 | 2017-11-08 | Clara Foods Co | Metodos y composiciones para produccion de proteina de clara de huevo. |
| CN105316362B (zh) * | 2015-08-19 | 2020-03-17 | 暨南大学 | 一种Dual-RMCE介导的TCR基因置换系统及其方法 |
| WO2017040343A1 (en) | 2015-08-28 | 2017-03-09 | Pioneer Hi-Bred International, Inc. | Ochrobactrum-mediated transformation of plants |
| WO2017201311A2 (en) | 2016-05-18 | 2017-11-23 | Amyris, Inc. | Compositions and methods for genomic integration of nucleic acids into exogenous landing pads |
| CA3146649A1 (en) | 2019-07-11 | 2021-01-14 | Clara Foods Co. | Protein compositions and consumable products thereof |
| US10927360B1 (en) | 2019-08-07 | 2021-02-23 | Clara Foods Co. | Compositions comprising digestive enzymes |
| WO2023015822A1 (zh) * | 2021-08-13 | 2023-02-16 | 上海鑫湾生物科技有限公司 | 缺氧触发的人工转录因子、转录控制系统及其应用 |
| AU2022424476A1 (en) | 2021-12-27 | 2024-07-11 | Repli-Tech Co.,Ltd. | Temperature-sensitive negative-strand RNA virus or virus vector and RNA genome thereof |
| CN117165627A (zh) * | 2022-05-27 | 2023-12-05 | 上海科技大学 | 基于Cre-LoxP和CRISPR的核酸构建体及其应用 |
| CN118147146B (zh) * | 2024-03-25 | 2024-09-17 | 中国科学院水生生物研究所 | 突变型LoxP元件、其表达载体和在鱼类基因工程育种中的应用 |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6228646B1 (en) * | 1996-03-07 | 2001-05-08 | The Regents Of The University Of California | Helper-free, totally defective adenovirus for gene therapy |
| AU4984697A (en) * | 1996-10-11 | 1998-05-11 | The Texas A & M University System | Methods for the generation of primordial germ cells and transgenic animal species |
-
1998
- 1998-09-28 JP JP27315098A patent/JP4206154B2/ja not_active Expired - Lifetime
- 1998-11-12 AU AU10526/99A patent/AU741415B2/en not_active Ceased
- 1998-11-12 WO PCT/JP1998/005094 patent/WO1999025851A1/ja active IP Right Grant
- 1998-11-12 AT AT98953027T patent/ATE412759T1/de not_active IP Right Cessation
- 1998-11-12 NZ NZ504307A patent/NZ504307A/en not_active IP Right Cessation
- 1998-11-12 CN CNB988127237A patent/CN1173039C/zh not_active Expired - Lifetime
- 1998-11-12 EP EP98953027A patent/EP1035208B1/en not_active Expired - Lifetime
- 1998-11-12 CA CA2309386A patent/CA2309386C/en not_active Expired - Fee Related
- 1998-11-12 KR KR1020007005138A patent/KR100639088B1/ko not_active IP Right Cessation
- 1998-11-12 DE DE69840173T patent/DE69840173D1/de not_active Expired - Lifetime
- 1998-11-12 US US09/554,271 patent/US6465254B1/en not_active Expired - Lifetime
- 1998-11-12 DK DK98953027T patent/DK1035208T3/da active
Also Published As
| Publication number | Publication date |
|---|---|
| KR100639088B1 (ko) | 2006-10-27 |
| US6465254B1 (en) | 2002-10-15 |
| ATE412759T1 (de) | 2008-11-15 |
| CN1173039C (zh) | 2004-10-27 |
| DE69840173D1 (de) | 2008-12-11 |
| KR20010032020A (ko) | 2001-04-16 |
| JPH11196880A (ja) | 1999-07-27 |
| EP1035208A1 (en) | 2000-09-13 |
| EP1035208B1 (en) | 2008-10-29 |
| WO1999025851A1 (fr) | 1999-05-27 |
| CA2309386C (en) | 2010-07-13 |
| CN1283233A (zh) | 2001-02-07 |
| JP4206154B2 (ja) | 2009-01-07 |
| CA2309386A1 (en) | 1999-05-27 |
| AU741415B2 (en) | 2001-11-29 |
| AU1052699A (en) | 1999-06-07 |
| NZ504307A (en) | 2001-07-27 |
| EP1035208A4 (en) | 2002-01-30 |
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