CA2351553A1 - Transgenic animals - Google Patents

Transgenic animals Download PDF

Info

Publication number
CA2351553A1
CA2351553A1 CA002351553A CA2351553A CA2351553A1 CA 2351553 A1 CA2351553 A1 CA 2351553A1 CA 002351553 A CA002351553 A CA 002351553A CA 2351553 A CA2351553 A CA 2351553A CA 2351553 A1 CA2351553 A1 CA 2351553A1
Authority
CA
Canada
Prior art keywords
present
animals
transgenic animals
oocytes
methods
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002351553A
Other languages
French (fr)
Other versions
CA2351553C (en
Inventor
Robert D. Bremel
Gregory T. Bleck
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Gala Design Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2351553A1 publication Critical patent/CA2351553A1/en
Application granted granted Critical
Publication of CA2351553C publication Critical patent/CA2351553C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/101—Bovine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/01—Animal expressing industrially exogenous proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2730/00—Reverse transcribing DNA viruses
    • C12N2730/00011—Details
    • C12N2730/10011—Hepadnaviridae
    • C12N2730/10111—Orthohepadnavirus, e.g. hepatitis B virus
    • C12N2730/10122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Environmental Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Animal Husbandry (AREA)
  • Virology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The present invention provides improved methods and compositions for the generation of transgenic non-human animals. The present invention permits the introduction of exogenous 5nucleic acid sequences into the genome of unfertilized eggs (e.g., pre-maturation oocytes and pre-fertilization oocytes) by microinjection of infectious retrovirus into the perivitelline space of the egg. The methods of the present invention provide an increased efficiency of production of transgenic animals with a reduced rate of generating animals which are mosaic for the presence of the transgene.
CA002351553A 1998-11-19 1999-11-17 Transgenic animals Expired - Fee Related CA2351553C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US19667098A 1998-11-19 1998-11-19
US09/196,670 1998-11-19
PCT/US1999/026848 WO2000030437A1 (en) 1998-11-19 1999-11-17 Transgenic animals

Publications (2)

Publication Number Publication Date
CA2351553A1 true CA2351553A1 (en) 2000-06-02
CA2351553C CA2351553C (en) 2007-01-30

Family

ID=22726354

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002351553A Expired - Fee Related CA2351553C (en) 1998-11-19 1999-11-17 Transgenic animals

Country Status (5)

Country Link
EP (1) EP1130960A4 (en)
AU (1) AU772439B2 (en)
CA (1) CA2351553C (en)
NZ (1) NZ511808A (en)
WO (1) WO2000030437A1 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010036978A2 (en) 2008-09-25 2010-04-01 Transgenrx, Inc. Novel vectors for production of growth hormone
US9150880B2 (en) 2008-09-25 2015-10-06 Proteovec Holding, L.L.C. Vectors for production of antibodies
WO2010118360A1 (en) 2009-04-09 2010-10-14 The Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College Production of proteins using transposon-based vectors
US9248179B2 (en) * 2010-06-24 2016-02-02 The United States of America, as represented by the Secretary of the Department of Health and Human Services, Centers for Disease Control and Prevention Pan-lyssavirus vaccines against rabies
CN108070617A (en) * 2016-11-16 2018-05-25 中国科学院海洋研究所 A kind of exopalaemon carinicauda embryo micro-injection method and Application way structure mRNA are overexpressed model
CN112342238A (en) * 2020-10-21 2021-02-09 复旦大学 Ovum function restoring preparation for inducing ovum maturation disorder by TRIP13 gene mutation and using method thereof

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8901778D0 (en) * 1989-01-27 1989-03-15 Univ Court Of The University O Manipulatory technique
US5453366A (en) * 1990-07-26 1995-09-26 Sims; Michele M. Method of cloning bovine embryos
US5550034A (en) * 1992-12-04 1996-08-27 Arch Development Corp. Apolipoprotein B mRNA editing protein compositions and methods
WO1994018834A1 (en) * 1993-02-16 1994-09-01 Virginia Tech Intellectual Properties, Inc. Polyelectrolyte dna conjugation and genetic transformation of an animal
US6080912A (en) * 1997-03-20 2000-06-27 Wisconsin Alumni Research Foundation Methods for creating transgenic animals

Also Published As

Publication number Publication date
CA2351553C (en) 2007-01-30
WO2000030437A8 (en) 2001-04-19
EP1130960A4 (en) 2003-07-30
WO2000030437A9 (en) 2001-05-17
WO2000030437A1 (en) 2000-06-02
AU772439B2 (en) 2004-04-29
NZ511808A (en) 2004-01-30
AU1721000A (en) 2000-06-13
EP1130960A1 (en) 2001-09-12

Similar Documents

Publication Publication Date Title
DE69839905D1 (en) METHOD FOR CREATING TRANSGENIC NON-HUMAN ANIMALS
US7932431B2 (en) Genetically modified non-human mammals and cells
Robl et al. Transgenic animal production and animal biotechnology
Kranz et al. An improved Flp deleter mouse in C57Bl/6 based on Flpo recombinase
IL135604A0 (en) Vectors comprising a magnum-specific promoter for avian transgenesis
WO2004067713A3 (en) Exogenous proteins expressed in avians and their eggs
Huang et al. In vivo transfection of testicular germ cells and transgenesis by using the mitochondrially localized jellyfish fluorescent protein gene
AU2002341746A1 (en) Production of a transgenic avian by cytoplasmic injection
AU2003299959A1 (en) Administration of transposon-based vectors to reproductive organs
DK0748386T3 (en) Generation of fibrinogen in transgenic animals
ATE281516T1 (en) METHOD FOR PRODUCING MAMMALS WITH DEFINED GENETIC CHARACTERISTICS
US7700356B2 (en) System for gene targeting and producing stable genomic transgene insertions
CA2351553A1 (en) Transgenic animals
Keskintepe et al. Derivation and comparison of C57BL/6 embryonic stem cells to a widely used 129 embryonic stem cell line
US20050273873A1 (en) Genomic modification
Ohtsuka et al. One-step generation of multiple transgenic mouse lines using an i mproved Pronuclear Injection-based Targeted Transgenesis (i-PITT)
WO2005038001A3 (en) Improved transgenesis by sperm-mediated gene transfer
WO2003024200A8 (en) Production of transgenic birds using stage x primordial germ cells
WO2000075300A3 (en) Methods for manipulating the avian genome
WO2001032008A8 (en) Methods for sexing non-human mammals
WO2002020752A3 (en) Cloned cells, embryos, and avians and methods, of producing them
ATE493498T1 (en) MODIFICATION OF THE PLANT GENOME THROUGH INDUCIBLE, SITE-SPECIFIC RECOMBINATION OF TRANSGENES
US20210345592A1 (en) Genetically modified sterile avians and method for the reconstitution thereof
WO2002067669A3 (en) Primordial germ cell-based germ line production of birds
EP1712126A4 (en) Transgenic bird and method of constructing the same

Legal Events

Date Code Title Description
EEER Examination request
MKLA Lapsed