WO2000075300A3 - Methods for manipulating the avian genome - Google Patents

Methods for manipulating the avian genome Download PDF

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Publication number
WO2000075300A3
WO2000075300A3 PCT/US2000/040059 US0040059W WO0075300A3 WO 2000075300 A3 WO2000075300 A3 WO 2000075300A3 US 0040059 W US0040059 W US 0040059W WO 0075300 A3 WO0075300 A3 WO 0075300A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
nucleic acid
manipulating
avian
avian genome
Prior art date
Application number
PCT/US2000/040059
Other languages
French (fr)
Other versions
WO2000075300A2 (en
Inventor
Paul A Ditullio
Karl M Ebert
Original Assignee
Tranxenogen Inc
Paul A Ditullio
Karl M Ebert
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Tranxenogen Inc, Paul A Ditullio, Karl M Ebert filed Critical Tranxenogen Inc
Priority to JP2001502566A priority Critical patent/JP2003501083A/en
Priority to NZ516173A priority patent/NZ516173A/en
Priority to CA002375441A priority patent/CA2375441A1/en
Priority to AU57898/00A priority patent/AU777420B2/en
Priority to EP00943424A priority patent/EP1190042A2/en
Publication of WO2000075300A2 publication Critical patent/WO2000075300A2/en
Publication of WO2000075300A3 publication Critical patent/WO2000075300A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
    • A01K67/027—New breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/02—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from eggs
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/30—Bird
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/01—Animal expressing industrially exogenous proteins
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/02—Animal zootechnically ameliorated
    • A01K2267/025—Animal producing cells or organs for transplantation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/008—Vector systems having a special element relevant for transcription cell type or tissue specific enhancer/promoter combination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/46—Vector systems having a special element relevant for transcription elements influencing chromatin structure, e.g. scaffold/matrix attachment region, methylation free island
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/80—Vector systems having a special element relevant for transcription from vertebrates
    • C12N2830/85—Vector systems having a special element relevant for transcription from vertebrates mammalian
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/80—Vector systems having a special element relevant for transcription from vertebrates
    • C12N2830/90—Vector systems having a special element relevant for transcription from vertebrates avian

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Environmental Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Endocrinology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Toxicology (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Diabetes (AREA)
  • Plant Pathology (AREA)
  • Animal Husbandry (AREA)
  • Microbiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

The invention features a method for introducing a nucleic acid molecule into the genome of an avian species by contacting in vivo a blastodermal cell of a fertilized egg with the nucleic acid molecule, which nucleic acid is not associated with a viral coat protein. The invention also encompasses transgenic avian animals and methods of producing such transgenic animals.
PCT/US2000/040059 1999-06-04 2000-06-02 Methods for manipulating the avian genome WO2000075300A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
JP2001502566A JP2003501083A (en) 1999-06-04 2000-06-02 Methods for manipulating the bird genome
NZ516173A NZ516173A (en) 1999-06-04 2000-06-02 Methods for manipulating the avian genome
CA002375441A CA2375441A1 (en) 1999-06-04 2000-06-02 Methods for manipulating the avian genome
AU57898/00A AU777420B2 (en) 1999-06-04 2000-06-02 Methods for manipulating the avian genome
EP00943424A EP1190042A2 (en) 1999-06-04 2000-06-02 Methods for manipulating the avian genome

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US13776199P 1999-06-04 1999-06-04
US60/137,761 1999-06-04

Publications (2)

Publication Number Publication Date
WO2000075300A2 WO2000075300A2 (en) 2000-12-14
WO2000075300A3 true WO2000075300A3 (en) 2002-01-10

Family

ID=22478939

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/040059 WO2000075300A2 (en) 1999-06-04 2000-06-02 Methods for manipulating the avian genome

Country Status (6)

Country Link
EP (1) EP1190042A2 (en)
JP (1) JP2003501083A (en)
AU (1) AU777420B2 (en)
CA (1) CA2375441A1 (en)
NZ (1) NZ516173A (en)
WO (1) WO2000075300A2 (en)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2001284703B2 (en) 2000-08-03 2007-03-22 Therapeutic Human Polyclonals Inc. Production of humanized antibodies in transgenic animals
US20020108132A1 (en) * 2001-02-02 2002-08-08 Avigenics Inc. Production of a monoclonal antibody by a transgenic chicken
CA2456262C (en) 2001-08-13 2012-09-04 Embrex, Inc. Methods for injecting avian eggs
ATE513038T1 (en) * 2001-09-18 2011-07-15 Synageva Biopharma Corp PRODUCTION OF A TRANSGENIC BIRD USING CYTOPLASM INJECTION
AU2002341795A1 (en) * 2001-09-21 2003-04-01 Avigenics, Inc. Production of transgenic avians using sperm-mediated transfection
US20060191026A1 (en) 2005-02-18 2006-08-24 Origen Therapeutics, Inc. Tissue specific expression of antibodies in chickens
US7323618B2 (en) 2002-02-01 2008-01-29 Origen Therapeutics, Inc. Tissue specific expression of exogenous proteins in transgenic chickens
US7145057B2 (en) 2002-02-01 2006-12-05 Origen Therapeutics, Inc. Chimeric bird from embryonic stem cells
EP1480675A4 (en) * 2002-02-08 2005-10-26 Tranxenogen Inc Immunoglobulin purification
JP2006523438A (en) * 2003-04-15 2006-10-19 ユニバーシティ・オブ・ジョージア・リサーチ・ファウンデイション・インコーポレイテッド Avian gene recombination using chicken ovalbumin gene region
ES2473596T3 (en) 2003-07-15 2014-07-07 Therapeutic Human Polyclonals, Inc. Humanized immunoglobulin loci
ES2378407T3 (en) 2006-09-01 2012-04-12 Therapeutic Human Polyclonals, Inc. Increased expression of human or humanized immunoglobulin in non-human transgenic animals
KR101720394B1 (en) 2011-09-21 2017-03-27 후지레비오 가부시키가이샤 Antibody against affinity complex

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990003439A1 (en) * 1988-09-22 1990-04-05 Amgen Inc. Method of gene transfer into chickens and other avian species
EP0424044A1 (en) * 1989-10-16 1991-04-24 Merck & Co. Inc. Transgenic fowl expressing bovine growth hormone
GB2282139A (en) * 1993-09-24 1995-03-29 Univ Reading Introducing DNA into the germ line of birds
WO1999010505A2 (en) * 1997-08-22 1999-03-04 University Of Guelph Production of proteins in eggs
WO1999019472A1 (en) * 1997-10-16 1999-04-22 University Of Georgia Research Foundation, Inc. Vectors comprising a magnum-specific promoter for avian transgenesis
EP0919121A1 (en) * 1996-07-08 1999-06-02 Dnavec Research Inc. In vivo electroporation method for early animal embryo

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990003439A1 (en) * 1988-09-22 1990-04-05 Amgen Inc. Method of gene transfer into chickens and other avian species
EP0424044A1 (en) * 1989-10-16 1991-04-24 Merck & Co. Inc. Transgenic fowl expressing bovine growth hormone
GB2282139A (en) * 1993-09-24 1995-03-29 Univ Reading Introducing DNA into the germ line of birds
EP0919121A1 (en) * 1996-07-08 1999-06-02 Dnavec Research Inc. In vivo electroporation method for early animal embryo
WO1999010505A2 (en) * 1997-08-22 1999-03-04 University Of Guelph Production of proteins in eggs
WO1999019472A1 (en) * 1997-10-16 1999-04-22 University Of Georgia Research Foundation, Inc. Vectors comprising a magnum-specific promoter for avian transgenesis

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
BRAZOLOT C L ET AL: "EFFICIENT TRANSFECTION OF CHICKEN CELLS BY LIPOFECTION, AND INTRODUCTION OF TRANSFECTED BLASTODERMAL CELLS INTO THE EMBRYO", MOLECULAR REPRODUCTION AND DEVELOPMENT,NEW YORK, NY,US, vol. 30, 1991, pages 304 - 312, XP000974404 *
ETCHES R J ET AL: "STRATEGIES FOR THE PRODUCTION OF TRANSGENIC CHICKENS", METHODS IN MOLECULAR BIOLOGY,HUMANA PRESS INC., CLIFTON, NJ,US, vol. 62, 1997, pages 433 - 450, XP000974906 *
MOHAMMED SM, MORRISON S, WIMS L, TRINH KR, WILDEMAN AG, BONSELAAR J, ETCHES RJ.: "Deposition of genetically engineered human antibodies into the egg yolk of hens.", IMMUNOTECHNOLOGY. 1998 OCT;4(2):115-25., XP000992749 *
PERRY M M AND SANG H M: "Transgenesis in chickens", TRANSGENIC RESEARCH,GB,LONDON, vol. 2, no. 3, 1 May 1993 (1993-05-01), pages 125 - 133, XP002086865, ISSN: 0962-8819 *
PETITTE J N ET AL: "THE ORIGIN OF THE AVIAN GERM LINE AND TRANSGENESIS IN BIRDS", POULTRY SCIENCE,CHAMPAIGN, IL,US, vol. 76, no. 8, August 1997 (1997-08-01), pages 1084 - 1092, XP000997849, ISSN: 0032-5791 *
See also references of EP1190042A2 *
THORAVAL ET AL: "Germline transmission of exogenous genes in chickens using helper-free ecotropic avian leukosis virus-based vectors", TRANSGENIC RESEARCH,GB,LONDON, vol. 4, no. 6, 1 November 1995 (1995-11-01), pages 369 - 377, XP002095225, ISSN: 0962-8819 *

Also Published As

Publication number Publication date
AU777420B2 (en) 2004-10-14
WO2000075300A2 (en) 2000-12-14
NZ516173A (en) 2004-02-27
CA2375441A1 (en) 2000-12-14
AU5789800A (en) 2000-12-28
EP1190042A2 (en) 2002-03-27
JP2003501083A (en) 2003-01-14

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