WO2000030437A8 - Transgenic animals - Google Patents

Transgenic animals

Info

Publication number
WO2000030437A8
WO2000030437A8 PCT/US1999/026848 US9926848W WO0030437A8 WO 2000030437 A8 WO2000030437 A8 WO 2000030437A8 US 9926848 W US9926848 W US 9926848W WO 0030437 A8 WO0030437 A8 WO 0030437A8
Authority
WO
WIPO (PCT)
Prior art keywords
present
animals
transgenic animals
oocytes
methods
Prior art date
Application number
PCT/US1999/026848
Other languages
French (fr)
Other versions
WO2000030437A1 (en
WO2000030437A9 (en
Inventor
Robert D Bremel
Gregory T Bleck
Original Assignee
Wisconsin Alumni Res Found
Robert D Bremel
Gregory T Bleck
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to EP99960310A priority Critical patent/EP1130960A4/en
Application filed by Wisconsin Alumni Res Found, Robert D Bremel, Gregory T Bleck filed Critical Wisconsin Alumni Res Found
Priority to NZ511808A priority patent/NZ511808A/en
Priority to CA002351553A priority patent/CA2351553C/en
Priority to AU17210/00A priority patent/AU772439B2/en
Publication of WO2000030437A1 publication Critical patent/WO2000030437A1/en
Publication of WO2000030437A8 publication Critical patent/WO2000030437A8/en
Publication of WO2000030437A9 publication Critical patent/WO2000030437A9/en

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/101—Bovine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/01—Animal expressing industrially exogenous proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2730/00—Reverse transcribing DNA viruses
    • C12N2730/00011—Details
    • C12N2730/10011—Hepadnaviridae
    • C12N2730/10111—Orthohepadnavirus, e.g. hepatitis B virus
    • C12N2730/10122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Environmental Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Animal Husbandry (AREA)
  • Virology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The present invention provides improved methods and compositions for the generation of transgenic non-human animals. The present invention permits the introduction of exogenous 5nucleic acid sequences into the genome of unfertilized eggs (e.g., pre-maturation oocytes and pre-fertilization oocytes) by microinjection of infectious retrovirus into the perivitelline space of the egg. The methods of the present invention provide an increased efficiency of production of transgenic animals with a reduced rate of generating animals which are mosaic for the presence of the transgene.
PCT/US1999/026848 1998-11-19 1999-11-17 Transgenic animals WO2000030437A1 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
EP99960310A EP1130960A4 (en) 1998-11-19 1999-11-12 Transgenic animals
NZ511808A NZ511808A (en) 1998-11-19 1999-11-17 Transgenic bovine that produce the heterologous protein in milk
CA002351553A CA2351553C (en) 1998-11-19 1999-11-17 Transgenic animals
AU17210/00A AU772439B2 (en) 1998-11-19 1999-11-17 Transgenic animals

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US19667098A 1998-11-19 1998-11-19
US09/196,670 1998-11-19

Publications (3)

Publication Number Publication Date
WO2000030437A1 WO2000030437A1 (en) 2000-06-02
WO2000030437A8 true WO2000030437A8 (en) 2001-04-19
WO2000030437A9 WO2000030437A9 (en) 2001-05-17

Family

ID=22726354

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/026848 WO2000030437A1 (en) 1998-11-19 1999-11-17 Transgenic animals

Country Status (5)

Country Link
EP (1) EP1130960A4 (en)
AU (1) AU772439B2 (en)
CA (1) CA2351553C (en)
NZ (1) NZ511808A (en)
WO (1) WO2000030437A1 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010036976A2 (en) 2008-09-25 2010-04-01 Transgenrx, Inc. Novel vectors for production of antibodies
WO2010036978A2 (en) 2008-09-25 2010-04-01 Transgenrx, Inc. Novel vectors for production of growth hormone
EP2417263B1 (en) 2009-04-09 2015-09-23 ProteoVec Holding L.L.C. Production of proteins using transposon-based vectors
CN103068985A (en) * 2010-06-24 2013-04-24 美国政府(由卫生和人类服务部、疾病控制和预防中心的部长所代表) Pan-lyssavirus vaccines against rabies
CN108070617A (en) * 2016-11-16 2018-05-25 中国科学院海洋研究所 A kind of exopalaemon carinicauda embryo micro-injection method and Application way structure mRNA are overexpressed model
CN112342238A (en) * 2020-10-21 2021-02-09 复旦大学 Ovum function restoring preparation for inducing ovum maturation disorder by TRIP13 gene mutation and using method thereof

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8901778D0 (en) * 1989-01-27 1989-03-15 Univ Court Of The University O Manipulatory technique
US5453366A (en) * 1990-07-26 1995-09-26 Sims; Michele M. Method of cloning bovine embryos
US5550034A (en) * 1992-12-04 1996-08-27 Arch Development Corp. Apolipoprotein B mRNA editing protein compositions and methods
WO1994018834A1 (en) * 1993-02-16 1994-09-01 Virginia Tech Intellectual Properties, Inc. Polyelectrolyte dna conjugation and genetic transformation of an animal
US6080912A (en) * 1997-03-20 2000-06-27 Wisconsin Alumni Research Foundation Methods for creating transgenic animals

Also Published As

Publication number Publication date
AU772439B2 (en) 2004-04-29
AU1721000A (en) 2000-06-13
CA2351553A1 (en) 2000-06-02
WO2000030437A1 (en) 2000-06-02
NZ511808A (en) 2004-01-30
EP1130960A4 (en) 2003-07-30
CA2351553C (en) 2007-01-30
EP1130960A1 (en) 2001-09-12
WO2000030437A9 (en) 2001-05-17

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