DK0975807T3 - To-trins hybridisering og indfangning af et polynukleotid - Google Patents

To-trins hybridisering og indfangning af et polynukleotid

Info

Publication number
DK0975807T3
DK0975807T3 DK98920106T DK98920106T DK0975807T3 DK 0975807 T3 DK0975807 T3 DK 0975807T3 DK 98920106 T DK98920106 T DK 98920106T DK 98920106 T DK98920106 T DK 98920106T DK 0975807 T3 DK0975807 T3 DK 0975807T3
Authority
DK
Denmark
Prior art keywords
target polynucleotide
hybridization
hybridization conditions
capture
polynucleotide
Prior art date
Application number
DK98920106T
Other languages
Danish (da)
English (en)
Inventor
William G Weisburg
Michael M Becker
Jay H Shaw
Mehrdad Majlessi
Original Assignee
Gen Probe Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gen Probe Inc filed Critical Gen Probe Inc
Application granted granted Critical
Publication of DK0975807T3 publication Critical patent/DK0975807T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Electrical Control Of Air Or Fuel Supplied To Internal-Combustion Engine (AREA)
DK98920106T 1997-05-02 1998-05-01 To-trins hybridisering og indfangning af et polynukleotid DK0975807T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US4543097P 1997-05-02 1997-05-02
PCT/US1998/008853 WO1998050583A1 (fr) 1997-05-02 1998-05-01 Hybridation en deux temps et capture d'un polynucleotide

Publications (1)

Publication Number Publication Date
DK0975807T3 true DK0975807T3 (da) 2007-01-29

Family

ID=21937832

Family Applications (1)

Application Number Title Priority Date Filing Date
DK98920106T DK0975807T3 (da) 1997-05-02 1998-05-01 To-trins hybridisering og indfangning af et polynukleotid

Country Status (11)

Country Link
US (3) US6110678A (fr)
EP (1) EP0975807B1 (fr)
JP (1) JP4222635B2 (fr)
KR (1) KR20010012175A (fr)
AT (1) ATE340868T1 (fr)
AU (1) AU738708B2 (fr)
CA (1) CA2287570C (fr)
DE (1) DE69836012T2 (fr)
DK (1) DK0975807T3 (fr)
ES (1) ES2271994T3 (fr)
WO (1) WO1998050583A1 (fr)

Families Citing this family (205)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5714380A (en) 1986-10-23 1998-02-03 Amoco Corporation Closed vessel for isolating target molecules and for performing amplification
US6327410B1 (en) 1997-03-14 2001-12-04 The Trustees Of Tufts College Target analyte sensors utilizing Microspheres
US7622294B2 (en) 1997-03-14 2009-11-24 Trustees Of Tufts College Methods for detecting target analytes and enzymatic reactions
US20030027126A1 (en) 1997-03-14 2003-02-06 Walt David R. Methods for detecting target analytes and enzymatic reactions
ES2402947T3 (es) 1997-04-10 2013-05-10 Stichting Katholieke Universiteit University Medical Centre Nijmegen PCA3, genes PCA3 y métodos de uso
ATE340868T1 (de) * 1997-05-02 2006-10-15 Gen Probe Inc Zwei-schritt hybridisierung und einfang von einem polynukleotid
US6534273B2 (en) 1997-05-02 2003-03-18 Gen-Probe Incorporated Two-step hybridization and capture of a polynucleotide
US6849400B1 (en) 1997-07-23 2005-02-01 Gen-Probe Incorporated Methods for detecting and measuring spliced nucleic acids
US7115884B1 (en) 1997-10-06 2006-10-03 Trustees Of Tufts College Self-encoding fiber optic sensor
US7348181B2 (en) 1997-10-06 2008-03-25 Trustees Of Tufts College Self-encoding sensor with microspheres
WO1999055365A1 (fr) 1998-04-30 1999-11-04 Cornell Research Foundation, Inc. Vecteurs adenoviraux avec proteines a deux types de genes de fibre
US8337753B2 (en) 1998-05-01 2012-12-25 Gen-Probe Incorporated Temperature-controlled incubator having a receptacle mixing mechanism
EP1930078A1 (fr) 1998-05-01 2008-06-11 Gen-Probe Incorporated Procédé pour l'agitation du contenu d'un conteneur
EP1090293B2 (fr) 1998-06-24 2019-01-23 Illumina, Inc. Décodage de détecteurs matricels à microspheres
US6355431B1 (en) 1999-04-20 2002-03-12 Illumina, Inc. Detection of nucleic acid amplification reactions using bead arrays
US20060275782A1 (en) 1999-04-20 2006-12-07 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
US6620584B1 (en) 1999-05-20 2003-09-16 Illumina Combinatorial decoding of random nucleic acid arrays
US6544732B1 (en) 1999-05-20 2003-04-08 Illumina, Inc. Encoding and decoding of array sensors utilizing nanocrystals
US8080380B2 (en) 1999-05-21 2011-12-20 Illumina, Inc. Use of microfluidic systems in the detection of target analytes using microsphere arrays
US8481268B2 (en) 1999-05-21 2013-07-09 Illumina, Inc. Use of microfluidic systems in the detection of target analytes using microsphere arrays
US6623920B1 (en) 1999-07-09 2003-09-23 Gen-Probe Incorporated Detection of HIV-1 by nucleic acid amplification
US7604996B1 (en) 1999-08-18 2009-10-20 Illumina, Inc. Compositions and methods for preparing oligonucleotide solutions
ATE321857T1 (de) * 1999-09-29 2006-04-15 Diagnocure Inc Pca3 mrna in gutartigen und bösartigen prostatageweben
US6428957B1 (en) * 1999-11-08 2002-08-06 Agilent Technologies, Inc. Systems tools and methods of assaying biological materials using spatially-addressable arrays
JP2003516765A (ja) 1999-12-15 2003-05-20 ジェン−プローブ・インコーポレーテッド 鳥型結核菌（Ｍｙｃｏｂａｃｔｅｒｉｕｍａｖｉｕｍ）複合体種の検出のための方法および組成物
AU781192B2 (en) * 1999-12-17 2005-05-12 Biomerieux Sa Nucleic acid amplification and detection of mycobacterium species
US6664081B2 (en) 1999-12-17 2003-12-16 Gen-Probe Incorporated Nucleic acid amplification and detection of mycobacterium species
EP1990428B1 (fr) 2000-02-07 2010-12-22 Illumina, Inc. Procédés de détection d'acide nucléique utilisant un amorçage universel
US7955794B2 (en) 2000-09-21 2011-06-07 Illumina, Inc. Multiplex nucleic acid reactions
US6913884B2 (en) * 2001-08-16 2005-07-05 Illumina, Inc. Compositions and methods for repetitive use of genomic DNA
US7361488B2 (en) * 2000-02-07 2008-04-22 Illumina, Inc. Nucleic acid detection methods using universal priming
US6812005B2 (en) 2000-02-07 2004-11-02 The Regents Of The University Of California Nucleic acid detection methods using universal priming
US7582420B2 (en) 2001-07-12 2009-09-01 Illumina, Inc. Multiplex nucleic acid reactions
US8076063B2 (en) 2000-02-07 2011-12-13 Illumina, Inc. Multiplexed methylation detection methods
US6770441B2 (en) 2000-02-10 2004-08-03 Illumina, Inc. Array compositions and methods of making same
US20020039728A1 (en) * 2000-02-10 2002-04-04 Robert Kain Alternative substrates and formats for bead-based array of arrays
WO2001061043A2 (fr) 2000-02-16 2001-08-23 Illumina, Inc. Genotypage parallele de plusieurs echantillons preleves sur des patients
US6962906B2 (en) * 2000-03-14 2005-11-08 Active Motif Oligonucleotide analogues, methods of synthesis and methods of use
CA2402319A1 (fr) 2000-03-14 2001-09-20 Active Motif Analogues d'oligonucleotides, procedes de synthese et procedes de mise en oeuvre
US20040014644A1 (en) * 2000-03-14 2004-01-22 Vladimir Efimov Oligonucleotide analogues and methods of use for modulating gene expression
US7439016B1 (en) * 2000-06-15 2008-10-21 Digene Corporation Detection of nucleic acids by type-specific hybrid capture method
US6582920B2 (en) 2000-09-01 2003-06-24 Gen-Probe Incorporated Amplification of HIV-1 RT sequences for detection of sequences associated with drug-resistance mutations
CA2835978C (fr) 2000-09-01 2016-10-11 Gen-Probe Incorporated Amplification de sequences vih-1 pour la detection de sequences associees aux mutations de la resistance aux medicaments
DE60132924T2 (de) 2000-10-23 2009-03-05 Gen-Probe Inc., San Diego Zusammensetzungen und methoden zur detektion von humanem immundefizienz virus 2 (hiv-2)
US20040018491A1 (en) * 2000-10-26 2004-01-29 Kevin Gunderson Detection of nucleic acid reactions on bead arrays
DE10126630A1 (de) * 2001-05-31 2003-01-09 Peter Und Traudl Engelhorn Sti Verfahren zur Zellsortierung
EP1421095B1 (fr) * 2001-08-31 2013-10-09 Gen-Probe Incorporated Test de detection d'acides nucleiques du parvovirus b19 humain
US20030143527A1 (en) * 2001-10-09 2003-07-31 Venkatakrishna Shyamala Identification of oligonucleotides for the capture, detection and quantitation of hepatitis B viral DNA
EP1448800A4 (fr) * 2001-11-21 2007-05-16 Applera Corp Dosage biologique numerique
US20030175709A1 (en) * 2001-12-20 2003-09-18 Murphy George L. Method and system for depleting rRNA populations
AU2003215240A1 (en) 2002-02-14 2003-09-04 Illumina, Inc. Automated information processing in randomly ordered arrays
US7776524B2 (en) 2002-02-15 2010-08-17 Genzyme Corporation Methods for analysis of molecular events
DE10211321A1 (de) * 2002-03-14 2003-09-25 Gnothis Holding Sa Ecublens Verwendung von Abfangsonden beim Nachweis von Nukleinsäuren
US7211382B2 (en) * 2002-04-09 2007-05-01 Orchid Cellmark Inc. Primer extension using modified nucleotides
EP1520045A1 (fr) * 2002-06-03 2005-04-06 PamGene B.V. Nouveaux ensembles ordonn s d' chantillons haute densit et technique d'analyse d'analysat
KR20050010902A (ko) * 2002-06-12 2005-01-28 카이론 코포레이션 Ａ형 간염 바이러스 핵산의 포획, 검출 및 정량을 위한올리고뉴클레오티드의 동정방법
CA2486420C (fr) 2002-06-14 2014-04-15 Gen-Probe Incorporated Compositions et methodes de detection du virus de l'hepatite b
EP2977470B1 (fr) 2002-10-16 2018-01-03 Gen-Probe Incorporated Compositions et procédé pour la détection du virus du nil occidental
GB2395557A (en) * 2002-11-22 2004-05-26 Dynal Biotech Ltd Nucleic acid probes
AU2004209578A1 (en) * 2003-02-07 2004-08-19 Diagnocure Inc. Method to detect prostate cancer in a sample
AU2004276722B2 (en) 2003-05-19 2009-03-12 Gen-Probe Incorporated Compositions, methods and kits for determining the presence of trichomonas vaginalis in a test sample
CA2432365A1 (fr) * 2003-06-30 2004-12-30 Jack A. Schalken Methode specifique de detection du cancer de la prostate fondee sur le gene pca3 et trousses connexes
US20050059024A1 (en) * 2003-07-25 2005-03-17 Ambion, Inc. Methods and compositions for isolating small RNA molecules
EP2251442B9 (fr) 2003-12-19 2012-04-25 Gen-Probe Incorporated Compositions, méthodes et kits destinés à la détection des acides nucléiques du VIH-1 et du VIH-2
US20060266136A1 (en) * 2004-06-08 2006-11-30 Gnothis Holding Ag Bi-fluorophore marked probes for detecting nucleic acids
EP1778867B1 (fr) 2004-07-01 2010-04-28 Gen-Probe Incorporated Méthodes et compositions de détection d'acides nucléiques dans un échantillon biologique
ES2392445T3 (es) 2004-07-13 2012-12-10 Gen-Probe Incorporated Composiciones y métodos para la detección de ácido nucleico del virus de la hepatitis A
US7662562B2 (en) * 2004-08-10 2010-02-16 Becton, Dickinson And Company Method for rapid identification of microorganisms
US7981607B2 (en) 2004-08-27 2011-07-19 Esoterix Genetic Laboratories LLC Method for detecting recombinant event
CA2582055C (fr) 2004-09-30 2014-04-08 Gen-Probe Incorporated Test de detection et de quantification du vih-1
US9109256B2 (en) 2004-10-27 2015-08-18 Esoterix Genetic Laboratories, Llc Method for monitoring disease progression or recurrence
CA2491067A1 (fr) 2004-12-24 2006-06-24 Stichting Katholieke Universiteit Rapport entre arnm dans les sediments urinaires ou l'urine en tant que marqueur pronostique du cancer de la prostate
CA2597319C (fr) 2005-02-18 2014-09-30 Gen-Probe Incorporated Procede de preparation d'echantillons faisant appel a un choc alcalin
WO2006099255A2 (fr) 2005-03-10 2006-09-21 Gen-Probe Incorporated Systemes et procedes permettant d'effectuer des dosages afin de detecter ou de quantifier des analytes dans des echantillons
US20090264635A1 (en) * 2005-03-25 2009-10-22 Applera Corporation Methods and compositions for depleting abundant rna transcripts
US9777314B2 (en) 2005-04-21 2017-10-03 Esoterix Genetic Laboratories, Llc Analysis of heterogeneous nucleic acid samples
ES2381279T3 (es) * 2005-05-06 2012-05-24 Gen-Probe Incorporated Métodos y productos para la captura de ácido nucleico diana
AU2006244460B2 (en) 2005-05-06 2010-04-08 Gen-Probe Incorporated Compositions and assays to detect influenza virus A and B nucleic acids
CA2609206A1 (fr) * 2005-06-06 2006-12-14 Gen-Probe Incorporated Compositions, procedes et necessaires pour determiner la presence de chlamidophila pneumoniae dans un echantillon test
EP1937847A2 (fr) 2005-10-17 2008-07-02 Gen-Probe Incorporated Compositions et procédés destinés à détecter des acides nucléiques de legionella pneumophila
CA2626977A1 (fr) * 2005-10-27 2007-05-03 Rosetta Inpharmatics Llc Amplification d'acides nucleiques au moyen d'amorces non aleatoires
EP1957645B1 (fr) 2005-12-06 2010-11-17 Ambion Inc. Amorces de transcription inverse et procedes de conception
AU2007249286B2 (en) * 2006-05-12 2013-06-13 Gen-Probe Incorporated Compositions and methods to detect enterococci nucleic acid
EP2027293B1 (fr) * 2006-05-24 2014-11-19 Gen-Probe Incorporated Methode pour le lysage de mycobacterium et l'amplification de ses acides nucleiques
AU2013201413B2 (en) * 2006-05-24 2014-05-29 Gen-Probe Incorporated Probes and kits for determining the presence of mycobacterium tuberculosis complex organisms in a test sample and method of amplifying gram-positive bacteria and fungi employing capture probe
JP5152929B2 (ja) 2006-06-06 2013-02-27 ジェン−プロウブ インコーポレイテッド 核酸増幅法におけるタグ化オリゴヌクレオチドおよびその使用
EP2765202B1 (fr) * 2006-08-01 2017-02-01 Gen-Probe Incorporated Procédés de capture de cible non spécifique d'acides nucléiques
AU2007336839C1 (en) * 2006-12-21 2013-12-19 Gen-Probe Incorporated Methods and compositions for nucleic acid amplification
GB0701253D0 (en) 2007-01-23 2007-02-28 Diagnostics For The Real World Nucleic acid amplification and testing
EP1978111B1 (fr) 2007-04-02 2013-03-27 Gen-Probe Incorporated Compositions, kits et procédés associés pour la détection et/ou la surveillance de Pseudomonas aeruginosa
EP2067867A1 (fr) * 2007-12-03 2009-06-10 Siemens Aktiengesellschaft Procédé de concentration de molécules d'acide nucléique
JP5498954B2 (ja) 2007-12-21 2014-05-21 ジェン−プロウブ インコーポレイテッド 抗生剤耐性微生物の検出
JP5646455B2 (ja) 2008-04-21 2014-12-24 ジェン−プロウブ インコーポレイテッド チクングニヤウイルスを検出するための方法
CA2723726C (fr) 2008-05-13 2017-09-12 Michael M. Becker Oligomeres de capture de cible inactivables pour hybridation et capture selectives de sequences d'acides nucleiques cibles
AU2009262870B2 (en) 2008-05-30 2014-03-20 Gen-Probe Incorporated Compositions, kits and related methods for the detection and/or monitoring of Salmonella
AU2009313808B2 (en) * 2008-11-14 2013-05-16 Gen-Probe Incorporated Compositions, kits and methods for detection of campylobacter nucleic acid
JP2012509675A (ja) * 2008-11-25 2012-04-26 ジェン−プロウブ インコーポレイテッド 低分子ｒｎａを検出するための組成物および方法、ならびにその使用
EP2358903A1 (fr) * 2008-12-10 2011-08-24 Smiths Detection Inc. Identification et différenciation d'une séquence d'acides nucléiques utilisant une hybridation dépendant de la température
EP2910647A1 (fr) 2008-12-30 2015-08-26 Gen-Probe Incorporated Composition, kits et méthodes correspondantes pour la détection at/ou la surveillance de listeria
US8921039B2 (en) 2009-02-26 2014-12-30 Gen-Probe Incorporated Assay for detection of human parvovirus nucleic acid
CA2768768C (fr) 2009-06-23 2021-08-24 Gen-Probe Incorporated Compositions et procedes pour detecter un acide nucleique a partir de mollicutes
WO2011003020A1 (fr) 2009-07-01 2011-01-06 Gen-Probe Incorporated Procédés et compositions pour l'amplification d'acide nucléique
AU2010286368B2 (en) 2009-08-31 2015-09-03 Gen-Probe Incorporated Dengue virus assay
CA2787327C (fr) 2010-01-22 2015-09-15 Damon Kittredge Getman Sondes pour detecter la presence de trichomonas vaginalis dans un echantillon
US9181593B2 (en) 2010-02-17 2015-11-10 Gen-Probe Incorporated Compositions and methods to detect Atopobium vaginae nucleic acid
WO2011133811A2 (fr) 2010-04-21 2011-10-27 Gen-Probe Incorporated Compositions, méthodes et kits permettant de détecter l'acide nucléique du virus de l'herpès simplex
US9234249B2 (en) 2010-07-12 2016-01-12 Gen-Probe Incorporated Compositions and assays to detect swine H1N1 influenza A virus, seasonal H1 influenza A virus and seasonal H3 influenza A virus nucleic acids
CN103675303B (zh) 2010-07-23 2016-02-03 贝克曼考尔特公司 传感器系统
US9046507B2 (en) 2010-07-29 2015-06-02 Gen-Probe Incorporated Method, system and apparatus for incorporating capacitive proximity sensing in an automated fluid transfer procedure
WO2012030856A2 (fr) 2010-08-30 2012-03-08 Gen-Probe Incorporated Compositions, procédés et mélanges réactionnels pour détection du virus xénotrope apparenté aux virus de la leucémie murine
WO2012037531A1 (fr) 2010-09-16 2012-03-22 Gen-Probe Incorporated Sondes de capture immobilisables par l'intermédiaire d'une queue nucléotidique l
WO2012046219A2 (fr) 2010-10-04 2012-04-12 Gen-Probe Prodesse, Inc. Compositions, méthodes et kits pour détecter des acides nucléiques adénoviraux
EP2635703B1 (fr) 2010-11-01 2018-03-21 Gen-Probe Incorporated Capture et amplification integrees d'acides nucleiques en vue de sequencage
EP2678664B1 (fr) 2011-02-24 2019-08-07 Gen-Probe Incorporated Systèmes et procédés de distinction de signaux optiques de différentes fréquences de modulation dans un détecteur de signaux optiques
AU2012236109A1 (en) * 2011-04-01 2013-11-21 Occam Biolabs, Inc. Methods and kits for detecting cell-free pathogen-specific nucleic acids
EP3604559A1 (fr) * 2011-04-25 2020-02-05 Gen-Probe Incorporated Compositions et procédés de détection d'acide nucléique bactérien associé au bv
US9752201B2 (en) 2011-07-15 2017-09-05 Gen-Probe Incorporated Compositions and method for detecting human parvovirus nucleic acid and for detecting hepatitis A virus nucleic acids in single-plex or multiplex assays
US10767208B2 (en) 2011-09-06 2020-09-08 Gen-Probe Incorporated Closed nucleic acid structures
EP2753713B1 (fr) 2011-09-08 2017-07-19 Gen-Probe Incorporated Compositions et procédés de détection d'acide nucléique bactérien associé à la vaginose bactérienne
US9175337B2 (en) 2011-09-21 2015-11-03 Gen-Probe Incorporated Methods for amplifying nucleic acid using tag-mediated displacement
US10152569B2 (en) 2011-09-26 2018-12-11 Gen-Probe Incorporated Algorithms for sequence determinations
US9482684B2 (en) 2011-11-07 2016-11-01 Beckman Coulter, Inc. Centrifuge system and workflow
EP2776844B1 (fr) 2011-11-07 2020-09-30 Beckman Coulter, Inc. Détection de récipient d'échantillon
US9446418B2 (en) 2011-11-07 2016-09-20 Beckman Coulter, Inc. Robotic arm
EP2776846B1 (fr) 2011-11-07 2019-08-21 Beckman Coulter, Inc. Système d'aliquote et flux de travail
JP2014532881A (ja) 2011-11-07 2014-12-08 ベックマン コールター， インコーポレイテッド 標本輸送システムのための磁気制動
ES2778054T3 (es) 2011-11-07 2020-08-07 Beckman Coulter Inc Sistema y método para transportar recipientes de muestras
DE102011120550B4 (de) 2011-12-05 2013-11-07 Gen-Probe Prodesse, Inc. Zusammensetzungen, Verfahren und Kits zur Detektion von Adenovirusnukleinsäuren
EP3511426B1 (fr) 2012-02-01 2022-08-17 Gen-Probe Incorporated Oligomères de capture de cibles en épingle à cheveux asymétriques
US11098375B2 (en) 2012-02-24 2021-08-24 Gen-Probe Prodesse, Inc. Detection of Shiga toxin genes in bacteria
JP6196611B2 (ja) * 2012-03-05 2017-09-13 日本碍子株式会社 標的核酸の検出方法
AU2013205110B2 (en) 2012-04-24 2016-10-13 Gen-Probe Incorporated Compositions, Methods and Kits to Detect Herpes Simplex Virus Nucleic Acids
AU2013205064B2 (en) 2012-06-04 2015-07-30 Gen-Probe Incorporated Compositions and Methods for Amplifying and Characterizing HCV Nucleic Acid
AU2013205087B2 (en) 2012-07-13 2016-03-03 Gen-Probe Incorporated Method for detecting a minority genotype
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
WO2014028537A1 (fr) 2012-08-14 2014-02-20 10X Technologies, Inc. Compositions de microcapsule et procédés
US9388465B2 (en) 2013-02-08 2016-07-12 10X Genomics, Inc. Polynucleotide barcode generation
US20150376609A1 (en) 2014-06-26 2015-12-31 10X Genomics, Inc. Methods of Analyzing Nucleic Acids from Individual Cells or Cell Populations
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US11591637B2 (en) * 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10400280B2 (en) 2012-08-14 2019-09-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9139870B2 (en) 2012-08-30 2015-09-22 Gen-Probe Incorporated Multiphase nucleic acid amplification
AU2013205122B2 (en) 2012-10-11 2016-11-10 Gen-Probe Incorporated Compositions and Methods for Detecting Human Papillomavirus Nucleic Acid
AU2013205090B2 (en) 2012-12-07 2016-07-28 Gen-Probe Incorporated Compositions and Methods for Detecting Gastrointestinal Pathogen Nucleic Acid
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
WO2014093676A1 (fr) 2012-12-14 2014-06-19 10X Technologies, Inc. Procédés et systèmes pour le traitement de polynucléotides
ES2934684T3 (es) 2013-03-15 2023-02-24 Abbott Lab Analizadores de diagnóstico automatizados que tienen carruseles dispuestos verticalmente y métodos relacionados
CN114137240A (zh) 2013-03-15 2022-03-04 雅培制药有限公司 具有后面可进入轨道系统的自动化诊断分析仪及相关方法
WO2014149118A2 (fr) 2013-03-15 2014-09-25 Abbott Laboratories Analyseurs de diagnostic dotés de carrousels de prétraitement et méthodes associées
EP4299767A3 (fr) 2013-08-14 2024-03-27 Gen-Probe Incorporated Compositions et procédés de détection de l'acide nucléique du hev
WO2015070173A1 (fr) * 2013-11-08 2015-05-14 Isis Pharmaceuticals, Inc. Composés et procédés permettant de détecter des oligonucléotides
CN106133145B (zh) 2014-02-28 2020-04-14 简·探针公司 从含有甲醛的液基细胞学防腐剂中的样本分离核酸的方法
GB2559919B (en) 2014-02-28 2018-11-14 Gen Probe Inc Method of isolating nucleic acid from specimens in liquid-based cytology preservatives containing formaldehyde
AU2015243445B2 (en) 2014-04-10 2020-05-28 10X Genomics, Inc. Fluidic devices, systems, and methods for encapsulating and partitioning reagents, and applications of same
EP3739062B1 (fr) 2014-10-20 2023-08-16 Gen-Probe Incorporated Solution de lyse cellulaire contenant des globules rouges
US10865433B2 (en) 2015-01-09 2020-12-15 Gen-Probe Incorporated Reaction mixtures for diagnosing bacterial vaginosis
CN112126675B (zh) 2015-01-12 2022-09-09 10X基因组学有限公司 用于制备核酸测序文库的方法和系统以及用其制备的文库
EP4286516A3 (fr) 2015-02-24 2024-03-06 10X Genomics, Inc. Procédés et systèmes de traitement de cloisonnement
US10550438B2 (en) 2015-03-16 2020-02-04 Gen-Probe Incorporated Methods and compositions for detecting bacterial nucleic acid
CN106868098A (zh) * 2015-12-10 2017-06-20 益善生物技术股份有限公司 循环肿瘤细胞分型鉴定试剂盒
JP6894439B2 (ja) 2016-01-04 2021-06-30 ジェン−プローブ・インコーポレーテッド Ｃａｎｄｉｄａ種を検出するための方法及び組成物
DE202017007130U1 (de) 2016-04-27 2019-08-29 Gen-Probe Inc. Lysereagenz für Blutzellen
EP3469103B1 (fr) 2016-06-10 2021-10-06 Gen-Probe Incorporated Compositions et procédés de détection d'acide nucléique du virus zika
WO2018013955A1 (fr) * 2016-07-15 2018-01-18 Northwestern University Compositions et procédés destinés à détecter les acides nucléiques dans des expectorations
CA3029274A1 (fr) 2016-09-15 2018-03-22 Sophie Laurenson Dispositifs et procedes d'analyse d'echantillon
CA3040907A1 (fr) 2016-10-19 2018-04-26 Gen-Probe Incorporated Compositions et methodes de detection ou quantification du virus de l'hepatite c
US10508313B2 (en) 2016-11-21 2019-12-17 Gen-Probe Incorporated Compositions and methods for detecting or quantifying Hepatitis B virus
US10427162B2 (en) 2016-12-21 2019-10-01 Quandx Inc. Systems and methods for molecular diagnostics
EP3545089B1 (fr) 2017-01-30 2022-03-09 10X Genomics, Inc. Procédés et systèmes de codage à barres de cellules individuelles sur la base de gouttelettes
US10799870B2 (en) 2017-03-03 2020-10-13 Gen-Probe Incorporated Evaporation-limiting inserts for reagent containers and related methods of use
JP6994513B2 (ja) 2017-03-24 2022-03-04 ジェン－プローブ・インコーポレーテッド 試料中のウイルス病原体を検出するための組成物および方法
EP3601618A1 (fr) 2017-03-25 2020-02-05 Gen-Probe Incorporated Compositions, procédés et kits pour détecter des acides nucléiques d'adénovirus, de métapneumovirus et/ou de rhinovirus
SG11201901822QA (en) 2017-05-26 2019-03-28 10X Genomics Inc Single cell analysis of transposase accessible chromatin
US10400235B2 (en) 2017-05-26 2019-09-03 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
AU2018281196B2 (en) 2017-06-07 2022-04-28 Gen-Probe Incorporated Detecting Babesia species nucleic acid in a sample
EP4289507A3 (fr) 2017-07-10 2024-02-28 Gen-Probe Incorporated Système analytique et méthodes pour l'amplification d'acides nucléics utilisant l'attribution de paramètres à l'échantillon
SG11201913654QA (en) 2017-11-15 2020-01-30 10X Genomics Inc Functionalized gel beads
US11377688B2 (en) 2017-11-17 2022-07-05 Gen-Probe Incorporated Compositions and methods for detecting C1orf43 nucleic acid
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
WO2019148169A1 (fr) 2018-01-29 2019-08-01 Gen-Probe Incorporated Systèmes et procédés analytiques
WO2019195166A1 (fr) 2018-04-06 2019-10-10 10X Genomics, Inc. Systèmes et procédés de contrôle de qualité dans un traitement de cellules uniques
GB2597569B (en) 2018-06-13 2023-01-25 Gen Probe Inc Compositions and methods for detecting group B streptococcus nucleic acid
EP3814496B1 (fr) 2018-06-28 2023-11-22 Gen-Probe Incorporated Méthode et système de préparation d'échantillon
US20210318324A1 (en) * 2018-07-05 2021-10-14 The Board Of Regents Of The University Of Oklahoma Gene signatures for cancer characterization and methods of use
EP3820616A1 (fr) 2018-07-10 2021-05-19 Gen-Probe Incorporated Procédés et systèmes de détection et de quantification d'acides nucléiques
AU2019314449A1 (en) 2018-08-01 2021-03-11 Gen-Probe Incorporated Compositions and methods for detecting nucleic acids of Epstein-Barr virus
US20210310059A1 (en) 2018-08-08 2021-10-07 Gen-Probe Incorporated Compositions, methods and kits for detecting mycoplasma genitalium
AU2019326462A1 (en) 2018-08-21 2021-03-25 Gen-Probe Incorporated Compositions and methods for amplifying, detecting or quantifying human cytomegalovirus
EP3841222A1 (fr) 2018-08-24 2021-06-30 Gen-Probe Incorporated Compositions et procédés pour détecter un acide nucléique bactérien et diagnostiquer une vaginose bactérienne
EP3856934A2 (fr) 2018-09-27 2021-08-04 Gen-Probe Incorporated Compositions et procédés de détection d'acides nucléiques de bordetella pertussis et de bordetella parapertussis
US20220017980A1 (en) 2018-10-01 2022-01-20 Gen-Probe Incorporated Compositions and methods for amplifying or detecting varicella-zoster virus
EP3870722A1 (fr) 2018-10-22 2021-09-01 Gen-Probe Incorporated Compositions et procédés d'amplification, de détection ou de quantification de polyomavirus bk humain
TW202030333A (zh) 2018-12-20 2020-08-16 美商簡 探針公司 用於檢測瘧原蟲物種核酸之組成物及方法
EP3914732A1 (fr) 2019-01-25 2021-12-01 Gen-Probe Incorporated Détection de mycoplasma genitalium résistant aux médicaments
AU2020299621A1 (en) 2019-07-03 2022-02-24 Gen-Probe Incorporated Oligonucleotides for use in determining the presence of Trichomonas vaginalis in a sample.
WO2021041056A1 (fr) 2019-08-23 2021-03-04 Gen-Probe Incorporated Compositions, procédés et kits pour la détection de treponema pallidum
AU2021230341A1 (en) 2020-03-04 2022-10-27 Gen-Probe Incorporated Compositions and methods for detecting SARS-CoV-2 nucleic acid
CN115698325A (zh) 2020-05-07 2023-02-03 盖立复诊断解决方案公司 用于检测SARS-CoV-2核酸的方法和组合物
CA3188657A1 (fr) 2020-07-17 2022-01-20 Gen-Probe Incorporated Detection du mycoplasma genitalium resistant aux macrolides
EP4337797A1 (fr) 2021-05-14 2024-03-20 Gen-Probe Incorporated Compositions et procédés de détection d'acide nucléique d'adénovirus humain
CA3220932A1 (fr) 2021-07-01 2023-01-05 Gen-Probe Incorporated Formulations enzymatiques et melanges reactionnels pour l'amplification d'acides nucleiques
WO2024054924A1 (fr) 2022-09-08 2024-03-14 Gen-Probe Incorporated Procédé de détection d'analytes d'acides nucléiques à l'aide d'amorces à double spécificité
WO2024081776A1 (fr) 2022-10-13 2024-04-18 Gen-Probe Incorporated Contrôle de la cellularité pour dosages d'acides nucléiques

Family Cites Families (52)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2422956A1 (fr) * 1978-04-13 1979-11-09 Pasteur Institut Procede de detection et de caracterisation d'un acide nucleique ou d'une sequence de celui-ci, et reactif enzymatique pour la mise en oeuvre de ce procede
FI63596C (fi) * 1981-10-16 1983-07-11 Orion Yhtymae Oy Mikrobdiagnostiskt foerfarande som grundar sig pao skiktshybridisering av nukleinsyror och vid foerfarandet anvaenda kombinationer av reagenser
US4554088A (en) 1983-05-12 1985-11-19 Advanced Magnetics Inc. Magnetic particles for use in separations
GB8401368D0 (en) 1984-01-19 1984-02-22 Amersham Int Plc Assay method
JPS61501746A (ja) 1984-04-05 1986-08-21 ライフ　テクノロジ−ズ，インコ−ポレイテツド 核酸の固定化法
US5288609A (en) * 1984-04-27 1994-02-22 Enzo Diagnostics, Inc. Capture sandwich hybridization method and composition
CA1260372A (fr) * 1984-04-27 1989-09-26 Elazar Rabbani Methode d'hybridation pour la detection de materiaux genetiques
US4755458A (en) * 1984-08-30 1988-07-05 Enzo Biochem, Inc. Composition and method for the detection of the presence of a polynucleotide sequence of interest
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US5386022A (en) 1985-03-28 1995-01-31 Hoffman-La Roche Inc. Primes and probes for the amplification and detection of aids associated nucleic acids
US5273882A (en) * 1985-06-13 1993-12-28 Amgen Method and kit for performing nucleic acid hybridization assays
US4751177A (en) * 1985-06-13 1988-06-14 Amgen Methods and kits for performing nucleic acid hybridization assays
US4868105A (en) * 1985-12-11 1989-09-19 Chiron Corporation Solution phase nucleic acid sandwich assay
US4882269A (en) 1985-12-13 1989-11-21 Princeton University Amplified hybridization assay
CA1284931C (fr) 1986-03-13 1991-06-18 Henry A. Erlich Procede de detection de variations specifiques de nucleotides et de polymorphismes genetiques dans les acides nucleiques
CA1308372C (fr) * 1986-08-11 1992-10-06 Geneva Ruth Davis Methode d'essai de type sandwich a sonde d'acide nucleique et compositions de ceux-ci
ZA877772B (en) * 1986-10-23 1988-04-20 Amoco Corporation Target and background capture methods and apparatus for affinity assays
US5750338A (en) * 1986-10-23 1998-05-12 Amoco Corporation Target and background capture methods with amplification for affinity assays
US5541308A (en) * 1986-11-24 1996-07-30 Gen-Probe Incorporated Nucleic acid probes for detection and/or quantitation of non-viral organisms
IL86724A (en) * 1987-06-19 1995-01-24 Siska Diagnostics Inc Methods and kits for amplification and testing of nucleic acid sequences
CA1340843C (fr) * 1987-07-31 1999-12-07 J. Lawrence Burg Amplification selective de sequences polynucleotidiques cibles
US5283174A (en) * 1987-09-21 1994-02-01 Gen-Probe, Incorporated Homogenous protection assay
US5639604A (en) 1987-09-21 1997-06-17 Gen-Probe Incorporated Homogeneous protection assay
US5124246A (en) * 1987-10-15 1992-06-23 Chiron Corporation Nucleic acid multimers and amplified nucleic acid hybridization assays using same
AU2735988A (en) * 1987-12-21 1989-07-13 Amoco Corporation Target and background capture methods with amplification for affinity assays
FR2625511B1 (fr) * 1987-12-31 1990-05-18 Sanofi Sa Procede d'obtention in vitro de tissus reconstitues
EP0436547B1 (fr) * 1988-05-10 1994-11-30 E.I. Du Pont De Nemours And Company Procede de detection rapide d'acide nucleique
US5130238A (en) * 1988-06-24 1992-07-14 Cangene Corporation Enhanced nucleic acid amplification process
GB8827160D0 (en) 1988-11-21 1988-12-29 Apothekernes Lab Detection & quantitative determination of rna & dna
CA2002076A1 (fr) * 1988-11-21 1990-05-21 Brent A. Burdick Trousse de diagnostic et methode utilisant un moyen de capture en phase solide pour la detection des acides nucleiques
AU640626B2 (en) 1988-11-21 1993-09-02 Dynal As Nucleic acid probes
US5449769A (en) * 1989-03-06 1995-09-12 Gen-Probe Incorporated Method and reagent for sulfurization of organophosphorous compounds
ATE118824T1 (de) * 1989-03-10 1995-03-15 Amoco Corp Immobilisierte oligonukleotidsonden und ihre verwendungen.
US5043272A (en) 1989-04-27 1991-08-27 Life Technologies, Incorporated Amplification of nucleic acid sequences using oligonucleotides of random sequence as primers
US5112734A (en) * 1989-05-26 1992-05-12 Gene-Trak Systems Target-dependent synthesis of an artificial gene for the synthesis of a replicatable rna
US5656207A (en) * 1989-06-24 1997-08-12 Gen Probe Incorporated Detecting or quantifying multiple analytes using labelling techniques
CA2020958C (fr) * 1989-07-11 2005-01-11 Daniel L. Kacian Methodes d'amplification de sequences d'acide nucleique
ATE152777T1 (de) * 1989-12-04 1997-05-15 Microprobe Corp Verstärkter einfang von ziel-nukleinsäuren mittels verwendung von oligonukleotiden, die kovalent an polymere gebunden sind
CA2032203C (fr) 1989-12-29 2009-05-19 Christine L. Brakel Essai de capture par amplification
US5200314A (en) 1990-03-23 1993-04-06 Chiron Corporation Polynucleotide capture assay employing in vitro amplification
WO1992015708A1 (fr) * 1991-02-27 1992-09-17 Amoco Corporation Procedes d'amelioration de la sensibilite d'essais d'hybridation
KR100249110B1 (ko) * 1992-05-06 2000-04-01 다니엘 엘. 캐시앙 핵산 서열 증폭 방법, 이에 이용되는 조성물 및 키트
KR100316498B1 (ko) * 1992-08-04 2002-06-20 다니엘 엘. 캐시앙, 헨리 엘. 노르호프, 피터 알. 쉬어리 핵산서열증폭방법
WO1994019023A1 (fr) * 1993-02-19 1994-09-01 Isis Pharmaceuticals, Inc. Oligonucleotides anti-sens contenant des cycles cyclobutyle, leurs procedes de preparation et d'utilisation
WO1995003430A1 (fr) * 1993-07-23 1995-02-02 Gen-Probe Incorporated Procede d'amelioration de l'amplification de l'acide nucleique
EP0648835A1 (fr) * 1993-10-14 1995-04-19 The Procter & Gamble Company Utilisation de sels alcalins de polyammonium pour augmenter la densité cationique des adoussinants textiles
US5681697A (en) * 1993-12-08 1997-10-28 Chiron Corporation Solution phase nucleic acid sandwich assays having reduced background noise and kits therefor
ATE340866T1 (de) * 1994-10-28 2006-10-15 Gen Probe Inc Zusammensetzungen und verfahren für die gleichzeitige detektion und quantifizierung von einer mehrheit spezifischer nuklein säure sequenzen
US5731153A (en) * 1996-08-26 1998-03-24 The Regents Of The University Of California Identification of random nucleic acid sequence aberrations using dual capture probes which hybridize to different chromosome regions
US5731148A (en) * 1995-06-07 1998-03-24 Gen-Probe Incorporated Adduct protection assay
US6060246A (en) 1996-11-15 2000-05-09 Avi Biopharma, Inc. Reagent and method for isolation and detection of selected nucleic acid sequences
ATE340868T1 (de) * 1997-05-02 2006-10-15 Gen Probe Inc Zwei-schritt hybridisierung und einfang von einem polynukleotid

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KR20010012175A (ko) 2001-02-15
JP4222635B2 (ja) 2009-02-12
CA2287570A1 (fr) 1998-11-12
EP0975807A1 (fr) 2000-02-02
ATE340868T1 (de) 2006-10-15
CA2287570C (fr) 2008-10-28
US20020028459A1 (en) 2002-03-07
US6280952B1 (en) 2001-08-28
DE69836012D1 (de) 2006-11-09
AU738708B2 (en) 2001-09-27
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DE69836012T2 (de) 2007-04-05
US6110678A (en) 2000-08-29

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