ES2171679T3 - Procedimiento de deteccion de secuencias de acido nucleico con recurso a una amplificacion competitiva. - Google Patents
Procedimiento de deteccion de secuencias de acido nucleico con recurso a una amplificacion competitiva.Info
- Publication number
- ES2171679T3 ES2171679T3 ES96917000T ES96917000T ES2171679T3 ES 2171679 T3 ES2171679 T3 ES 2171679T3 ES 96917000 T ES96917000 T ES 96917000T ES 96917000 T ES96917000 T ES 96917000T ES 2171679 T3 ES2171679 T3 ES 2171679T3
- Authority
- ES
- Spain
- Prior art keywords
- nucleic acid
- target
- acid sequence
- test sample
- resource
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6862—Ligase chain reaction [LCR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
- C12Q1/701—Specific hybridization probes
- C12Q1/706—Specific hybridization probes for hepatitis
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Communicable Diseases (AREA)
- Virology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
EN LA PRESENTE INVENCION SE PRESENTA UN METODO PARA DETECTAR DE FORMA CUANTITATIVA LA CANTIDAD DE UNA SECUENCIA DE ACIDO NUCLEICO OBJETIVO QUE SE PUEDE ENCONTRAR PRESENTE EN UNA MUESTRA DE ENSAYO. SE PONE EN CONTACTO UNA MUESTRA DE ENSAYO QUE PUEDE CONTENER UNA SECUENCIA DE ACIDO NUCLEICO OBJETIVO QUE CONTIENE SECUENCIAS OBJETIVO X E Y CON DOS GRUPOS DE IMPRIMADORES, SIENDO EL PRIMER GRUPO ESPECIFICO PARA EL OBJETIVO X Y EL SEGUNDO GRUPO ESPECIFICO PARA EL OBJETIVO Y. LA MUESTRA DE ENSAYO TAMBIEN SE PONE EN CONTACTO AL MISMO TIEMPO CON UNA SECUENCIA ESTANDAR INTERNA IS, DERIVADA SUSTANCIALMENTE DE UNA COMBINACION DE LA PRIMERA Y LA SEGUNDA SECUENCIAS OBJETIVO, Y SUS IMPRIMADORES OLIGONUCLEOTIDOS CORRESPONDIENTES. SE ASOCIAN UNOS HAPTENOS A LOS GRUPOS DE IMPRIMADORES OLIGONUCLEOTIDOS DE MODO QUE LOS PRODUCTOS DE LAS SECUENCIAS OBJETIVO AMPLIFICADAS X E Y SE PUEDAN DETECTAR POR CAPTURA EN UNA FASE SOLIDA A LA QUE ESTAN ACOPLADOS LOS REACTIVOS DE CAPTURA ANTIHAPTENOS. SE DETERMINA UNA RELACIONDE SEÑAL DE (X + Y)/S PARA CUANTIFICAR LA CANTIDAD DE LA SECUENCIA SE ACIDO NUCLEICO OBJETIVO PRESENTE EN LA MUESTRA.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US08/480,220 US5667974A (en) | 1995-06-07 | 1995-06-07 | Method for detecting nucleic acid sequences using competitive amplification |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2171679T3 true ES2171679T3 (es) | 2002-09-16 |
Family
ID=23907134
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES96917000T Expired - Lifetime ES2171679T3 (es) | 1995-06-07 | 1996-06-03 | Procedimiento de deteccion de secuencias de acido nucleico con recurso a una amplificacion competitiva. |
Country Status (8)
Country | Link |
---|---|
US (2) | US5667974A (es) |
EP (1) | EP0832281B1 (es) |
JP (1) | JPH11506613A (es) |
AT (1) | ATE211771T1 (es) |
CA (1) | CA2223823A1 (es) |
DE (1) | DE69618460T2 (es) |
ES (1) | ES2171679T3 (es) |
WO (1) | WO1996040996A1 (es) |
Families Citing this family (26)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6335160B1 (en) | 1995-02-17 | 2002-01-01 | Maxygen, Inc. | Methods and compositions for polypeptide engineering |
US6406855B1 (en) | 1994-02-17 | 2002-06-18 | Maxygen, Inc. | Methods and compositions for polypeptide engineering |
US5667974A (en) * | 1995-06-07 | 1997-09-16 | Abbott Laboratories | Method for detecting nucleic acid sequences using competitive amplification |
SE504798C2 (sv) * | 1995-06-16 | 1997-04-28 | Ulf Landegren | Immunanalys och testkit med två reagens som kan tvärbindas om de adsorberats till analyten |
US6852487B1 (en) * | 1996-02-09 | 2005-02-08 | Cornell Research Foundation, Inc. | Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays |
US20020150921A1 (en) * | 1996-02-09 | 2002-10-17 | Francis Barany | Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays |
CA2255774C (en) * | 1996-05-29 | 2008-03-18 | Cornell Research Foundation, Inc. | Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions |
US6289229B1 (en) | 1998-01-20 | 2001-09-11 | Scimed Life Systems, Inc. | Readable probe array for in vivo use |
US6506594B1 (en) * | 1999-03-19 | 2003-01-14 | Cornell Res Foundation Inc | Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays |
US7014994B1 (en) | 1999-03-19 | 2006-03-21 | Cornell Research Foundation,Inc. | Coupled polymerase chain reaction-restriction-endonuclease digestion-ligase detection reaction process |
AR028149A1 (es) * | 1999-07-08 | 2003-04-30 | Innogenetics Nv | Deteccion de la resistencia a los farmacos contra la hepatitis b |
US6759193B2 (en) * | 1999-07-28 | 2004-07-06 | The Government Of The Republic Of Singapore | Detection of human hepatitis B virus surface antigen mutants by specific amplification and its application on gene chip |
EP1233976A4 (en) * | 1999-11-17 | 2003-06-11 | Jiuping Ji | SIMULTANEOUS DETECTION OF HBV, HCV AND HIV IN PLASMA SAMPLES WITH THE AID OF A MULTIPLEX FIXING TEST |
ES2170620B1 (es) * | 1999-11-25 | 2003-12-16 | Univ Alcala Henares | Procedimiento de cuantificacion de acidos nucleicos mediante amplificacion por reaccion en cadena de polimerasa hemi-competitiva. |
AU2001293366A1 (en) | 2000-04-14 | 2001-10-30 | Cornell Research Foundation, Inc. | Method of designing addressable array for detection of nucleic acid sequence differences using ligase detection reaction |
SG90149A1 (en) * | 2000-07-18 | 2002-07-23 | Government Of The Republic Of | Diagnostic assay |
EP1513958B1 (en) | 2002-06-14 | 2011-08-10 | Gen-Probe Incorporated | Compositions for detecting hepatitis b virus |
KR100505419B1 (ko) * | 2003-04-23 | 2005-08-04 | 주식회사 하이닉스반도체 | 반도체 소자의 소자분리막 제조방법 |
EP2208797A3 (en) * | 2004-03-01 | 2010-11-24 | Applied Biosystems, LLC | Methods, compositions and kits for use in polynucleotide amplification |
JPWO2009054367A1 (ja) * | 2007-10-23 | 2011-03-03 | オリンパス株式会社 | 標的dnaの検出方法及び標的dna検出キット |
EP2205617A4 (en) * | 2007-11-06 | 2013-10-16 | Siemens Healthcare Diagnostics | SPECIFIC OLIGONUCLEOTIDE SEQUENCES OF HEPATITIS B VIRUS (HBV) |
US10114015B2 (en) | 2013-03-13 | 2018-10-30 | Meso Scale Technologies, Llc. | Assay methods |
KR20220038550A (ko) | 2013-03-13 | 2022-03-28 | 메소 스케일 테크놀러지즈, 엘엘시 | 개선된 분석 방법 |
AU2015259048B2 (en) | 2014-05-15 | 2021-09-09 | Meso Scale Technologies, Llc. | Improved assay methods |
AU2015319825A1 (en) * | 2014-09-26 | 2017-04-27 | Nooma Bio, Inc. | Target sequence detection by nanopore sensing of synthetic probes |
US11486873B2 (en) | 2016-03-31 | 2022-11-01 | Ontera Inc. | Multipore determination of fractional abundance of polynucleotide sequences in a sample |
Family Cites Families (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5219727A (en) * | 1989-08-21 | 1993-06-15 | Hoffmann-Laroche Inc. | Quantitation of nucleic acids using the polymerase chain reaction |
US5427930A (en) * | 1990-01-26 | 1995-06-27 | Abbott Laboratories | Amplification of target nucleic acids using gap filling ligase chain reaction |
CA2049879A1 (en) * | 1990-08-30 | 1992-03-01 | Keith C. Backman | Controlled initial target-dependent production of templates for ligase chain reaction |
GB9116042D0 (en) * | 1991-07-24 | 1991-09-11 | Royal Free Hosp School Med | Diagnostic method |
JPH07505293A (ja) * | 1992-03-31 | 1995-06-15 | アボツト・ラボラトリーズ | 多重リガーゼ連鎖反応方法 |
JPH05308999A (ja) * | 1992-05-08 | 1993-11-22 | Sumitomo Metal Ind Ltd | B型肝炎ウイルスpre−C変異の判定法 |
AU4848393A (en) * | 1992-09-25 | 1994-04-26 | Abbott Laboratories | Ligase chain reaction method for detecting small mutations |
CA2144433A1 (en) * | 1992-10-01 | 1994-04-14 | Thomas P. Gandek | Improved bleaching of high consistency lignocellulosic pulp |
EP0785996A1 (en) * | 1993-07-13 | 1997-07-30 | Abbott Laboratories | Nucleotide sequences and process for amplifying and detection of hepatitis b virus |
US5667974A (en) * | 1995-06-07 | 1997-09-16 | Abbott Laboratories | Method for detecting nucleic acid sequences using competitive amplification |
-
1995
- 1995-06-07 US US08/480,220 patent/US5667974A/en not_active Expired - Fee Related
-
1996
- 1996-06-03 AT AT96917000T patent/ATE211771T1/de not_active IP Right Cessation
- 1996-06-03 JP JP9501035A patent/JPH11506613A/ja active Pending
- 1996-06-03 ES ES96917000T patent/ES2171679T3/es not_active Expired - Lifetime
- 1996-06-03 WO PCT/US1996/008429 patent/WO1996040996A1/en active IP Right Grant
- 1996-06-03 EP EP96917000A patent/EP0832281B1/en not_active Expired - Lifetime
- 1996-06-03 CA CA002223823A patent/CA2223823A1/en not_active Abandoned
- 1996-06-03 DE DE69618460T patent/DE69618460T2/de not_active Expired - Fee Related
-
1997
- 1997-05-28 US US08/864,404 patent/US5955598A/en not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
DE69618460T2 (de) | 2002-09-26 |
EP0832281A1 (en) | 1998-04-01 |
ATE211771T1 (de) | 2002-01-15 |
WO1996040996A1 (en) | 1996-12-19 |
US5955598A (en) | 1999-09-21 |
CA2223823A1 (en) | 1996-12-19 |
US5667974A (en) | 1997-09-16 |
EP0832281B1 (en) | 2002-01-09 |
DE69618460D1 (de) | 2002-02-14 |
JPH11506613A (ja) | 1999-06-15 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FG2A | Definitive protection |
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