DE69618460D1 - Verfahren zum nachweis von nukleinsäuresequenzen unter verwendung von kompetitiven amplifikation - Google Patents

Verfahren zum nachweis von nukleinsäuresequenzen unter verwendung von kompetitiven amplifikation

Info

Publication number
DE69618460D1
DE69618460D1 DE69618460T DE69618460T DE69618460D1 DE 69618460 D1 DE69618460 D1 DE 69618460D1 DE 69618460 T DE69618460 T DE 69618460T DE 69618460 T DE69618460 T DE 69618460T DE 69618460 D1 DE69618460 D1 DE 69618460D1
Authority
DE
Germany
Prior art keywords
target
nucleic acid
acid sequence
test sample
acid sequences
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69618460T
Other languages
English (en)
Other versions
DE69618460T2 (de
Inventor
Larry Birkenmeyer
K Mushahwar
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Abbott Laboratories
Original Assignee
Abbott Laboratories
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Abbott Laboratories filed Critical Abbott Laboratories
Application granted granted Critical
Publication of DE69618460D1 publication Critical patent/DE69618460D1/de
Publication of DE69618460T2 publication Critical patent/DE69618460T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6862—Ligase chain reaction [LCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/706—Specific hybridization probes for hepatitis

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Communicable Diseases (AREA)
  • Virology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DE69618460T 1995-06-07 1996-06-03 Verfahren zum nachweis von nukleinsäuresequenzen unter verwendung von kompetitiven amplifikation Expired - Fee Related DE69618460T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/480,220 US5667974A (en) 1995-06-07 1995-06-07 Method for detecting nucleic acid sequences using competitive amplification
PCT/US1996/008429 WO1996040996A1 (en) 1995-06-07 1996-06-03 Method for detecting nucleic acid sequences using competitive amplification

Publications (2)

Publication Number Publication Date
DE69618460D1 true DE69618460D1 (de) 2002-02-14
DE69618460T2 DE69618460T2 (de) 2002-09-26

Family

ID=23907134

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69618460T Expired - Fee Related DE69618460T2 (de) 1995-06-07 1996-06-03 Verfahren zum nachweis von nukleinsäuresequenzen unter verwendung von kompetitiven amplifikation

Country Status (8)

Country Link
US (2) US5667974A (de)
EP (1) EP0832281B1 (de)
JP (1) JPH11506613A (de)
AT (1) ATE211771T1 (de)
CA (1) CA2223823A1 (de)
DE (1) DE69618460T2 (de)
ES (1) ES2171679T3 (de)
WO (1) WO1996040996A1 (de)

Families Citing this family (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6406855B1 (en) 1994-02-17 2002-06-18 Maxygen, Inc. Methods and compositions for polypeptide engineering
US6335160B1 (en) 1995-02-17 2002-01-01 Maxygen, Inc. Methods and compositions for polypeptide engineering
US5667974A (en) * 1995-06-07 1997-09-16 Abbott Laboratories Method for detecting nucleic acid sequences using competitive amplification
SE504798C2 (sv) * 1995-06-16 1997-04-28 Ulf Landegren Immunanalys och testkit med två reagens som kan tvärbindas om de adsorberats till analyten
US20020150921A1 (en) * 1996-02-09 2002-10-17 Francis Barany Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
US6852487B1 (en) 1996-02-09 2005-02-08 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
EP2369007B1 (de) 1996-05-29 2015-07-29 Cornell Research Foundation, Inc. Erkennung von Nukleinsäuresequenz-Unterschieden mittels der gleichzeitigen Ligaseerkennungs- und Polymerasekettenreaktion
US6289229B1 (en) 1998-01-20 2001-09-11 Scimed Life Systems, Inc. Readable probe array for in vivo use
US7014994B1 (en) 1999-03-19 2006-03-21 Cornell Research Foundation,Inc. Coupled polymerase chain reaction-restriction-endonuclease digestion-ligase detection reaction process
US6506594B1 (en) * 1999-03-19 2003-01-14 Cornell Res Foundation Inc Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
AR028149A1 (es) * 1999-07-08 2003-04-30 Innogenetics Nv Deteccion de la resistencia a los farmacos contra la hepatitis b
US6759193B2 (en) * 1999-07-28 2004-07-06 The Government Of The Republic Of Singapore Detection of human hepatitis B virus surface antigen mutants by specific amplification and its application on gene chip
AU1777401A (en) * 1999-11-17 2001-05-30 Xiuli Chen Simultaneous detection of hbv, hcv and hiv in plasma samples using a multiplex capture assay
ES2170620B1 (es) * 1999-11-25 2003-12-16 Univ Alcala Henares Procedimiento de cuantificacion de acidos nucleicos mediante amplificacion por reaccion en cadena de polimerasa hemi-competitiva.
AU2001293366A1 (en) * 2000-04-14 2001-10-30 Cornell Research Foundation, Inc. Method of designing addressable array for detection of nucleic acid sequence differences using ligase detection reaction
SG90149A1 (en) * 2000-07-18 2002-07-23 Government Of The Republic Of Diagnostic assay
CA2486420C (en) * 2002-06-14 2014-04-15 Gen-Probe Incorporated Compositions and methods for detecting hepatitis b virus
KR100505419B1 (ko) * 2003-04-23 2005-08-04 주식회사 하이닉스반도체 반도체 소자의 소자분리막 제조방법
EP2208797A3 (de) * 2004-03-01 2010-11-24 Applied Biosystems, LLC Verfahren, Zusammensetzungen und Kits für Polynukleotidamplifikation
JPWO2009054367A1 (ja) * 2007-10-23 2011-03-03 オリンパス株式会社 標的ｄｎａの検出方法及び標的ｄｎａ検出キット
EP2205617A4 (de) * 2007-11-06 2013-10-16 Siemens Healthcare Diagnostics Hepatitis-b-virus-spezifische oligonukleotidsequenzen
AU2014248759B2 (en) 2013-03-13 2020-02-27 Meso Scale Technologies, Llc. Improved assay methods
US10114015B2 (en) 2013-03-13 2018-10-30 Meso Scale Technologies, Llc. Assay methods
WO2015175856A1 (en) 2014-05-15 2015-11-19 Meso Scale Technologies, Llc. Improved assay methods
US20170349940A1 (en) * 2014-09-26 2017-12-07 Two Pore Guys, Inc. Targeted Sequence Detection by Nanopore Sensing of Synthetic Probes
US11486873B2 (en) 2016-03-31 2022-11-01 Ontera Inc. Multipore determination of fractional abundance of polynucleotide sequences in a sample

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5219727A (en) * 1989-08-21 1993-06-15 Hoffmann-Laroche Inc. Quantitation of nucleic acids using the polymerase chain reaction
US5427930A (en) * 1990-01-26 1995-06-27 Abbott Laboratories Amplification of target nucleic acids using gap filling ligase chain reaction
CA2049879A1 (en) * 1990-08-30 1992-03-01 Keith C. Backman Controlled initial target-dependent production of templates for ligase chain reaction
GB9116042D0 (en) * 1991-07-24 1991-09-11 Royal Free Hosp School Med Diagnostic method
WO1993020227A1 (en) * 1992-03-31 1993-10-14 Abbott Laboratories Method of multiplex ligase chain reaction
JPH05308999A (ja) * 1992-05-08 1993-11-22 Sumitomo Metal Ind Ltd Ｂ型肝炎ウイルスｐｒｅ−Ｃ変異の判定法
WO1994008047A1 (en) * 1992-09-25 1994-04-14 Abbott Laboratories Ligase chain reaction method for detecting small mutations
CA2144433A1 (en) * 1992-10-01 1994-04-14 Thomas P. Gandek Improved bleaching of high consistency lignocellulosic pulp
CA2167056A1 (en) * 1993-07-13 1995-01-26 Uwe Spies Nucleotide sequences and process for amplifying and detection of hepatitis b virus
US5667974A (en) * 1995-06-07 1997-09-16 Abbott Laboratories Method for detecting nucleic acid sequences using competitive amplification

Also Published As

Publication number Publication date
ES2171679T3 (es) 2002-09-16
US5667974A (en) 1997-09-16
CA2223823A1 (en) 1996-12-19
WO1996040996A1 (en) 1996-12-19
DE69618460T2 (de) 2002-09-26
US5955598A (en) 1999-09-21
JPH11506613A (ja) 1999-06-15
EP0832281A1 (de) 1998-04-01
EP0832281B1 (de) 2002-01-09
ATE211771T1 (de) 2002-01-15

Similar Documents

Publication Publication Date Title
ATE211771T1 (de) Verfahren zum nachweis von nukleinsäuresequenzen unter verwendung von kompetitiven amplifikation
ATE215993T1 (de) Nachweis von nukleinsäuren mit breitem dynamikbereich unter verwendung von aggregat- primer-reihen
MY104957A (en) Method detecting nucleotide sequences.
ATE142272T1 (de) Nukleinsäure-amplifikation unter verwendung eines einzelprimers
EP0374665A3 (de) Prüfung von Sequenzen mittels amplifizierter Gene
CA2236067A1 (en) Methods, apparatus and computer program products for determining quantities of nucleic acid sequences in samples
ATE236269T1 (de) Ein elektrochemiluminescenter nachweis von nukleinsäuren auf basis der selbsttragenden sequenzreplikation
DE59606675D1 (de) Verfahren zum nachweis von telomerase-aktivität
ATE184055T1 (de) Verfahren zum sensitiven nachweis von reverser transcriptase
ATE449868T1 (de) Methoden, testsätze und kompositionen zur detektion und quantifizierung von zielsequenzen
EP0950718A4 (de) Verfahren zur detektion von dna mittels einer pna-sonde
ATE232981T1 (de) Differenziertes timingsverfahren zum nachweiss multiple analyte in eine testprobe
DK0677589T3 (da) Fremgangsmåde, testelement og testkit til semikvntitativ påvisning af mål-nucleinsyre

Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee