DK0724639T3 - Fremgangsmåder til udvælgelse af rekombinante værtsceller, der udtrykker høje niveauer af et ønsket protein - Google Patents
Fremgangsmåder til udvælgelse af rekombinante værtsceller, der udtrykker høje niveauer af et ønsket proteinInfo
- Publication number
- DK0724639T3 DK0724639T3 DK92908635T DK92908635T DK0724639T3 DK 0724639 T3 DK0724639 T3 DK 0724639T3 DK 92908635 T DK92908635 T DK 92908635T DK 92908635 T DK92908635 T DK 92908635T DK 0724639 T3 DK0724639 T3 DK 0724639T3
- Authority
- DK
- Denmark
- Prior art keywords
- methods
- host cells
- high levels
- cells expressing
- desired protein
- Prior art date
Links
- 108090000623 proteins and genes Proteins 0.000 title abstract 6
- 102000004169 proteins and genes Human genes 0.000 title abstract 3
- 108091026890 Coding region Proteins 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/61—Growth hormone [GH], i.e. somatotropin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Endocrinology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Cell Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US67704591A | 1991-03-29 | 1991-03-29 | |
| PCT/US1992/001358 WO1992017566A2 (fr) | 1991-03-29 | 1992-02-20 | Procedes pour la selection de cellules hotes de recombinaison exprimant des niveaux eleves d'une proteine desiree |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK0724639T3 true DK0724639T3 (da) | 2001-03-05 |
Family
ID=24717083
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK92908635T DK0724639T3 (da) | 1991-03-29 | 1992-02-20 | Fremgangsmåder til udvælgelse af rekombinante værtsceller, der udtrykker høje niveauer af et ønsket protein |
Country Status (10)
| Country | Link |
|---|---|
| EP (1) | EP0724639B1 (fr) |
| JP (1) | JP3438889B2 (fr) |
| AT (1) | ATE199023T1 (fr) |
| AU (1) | AU660671B2 (fr) |
| CA (1) | CA2104598C (fr) |
| DE (1) | DE69231676T2 (fr) |
| DK (1) | DK0724639T3 (fr) |
| ES (1) | ES2153358T3 (fr) |
| GR (1) | GR3035599T3 (fr) |
| WO (1) | WO1992017566A2 (fr) |
Families Citing this family (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5561053A (en) * | 1994-08-05 | 1996-10-01 | Genentech, Inc. | Method for selecting high-expressing host cells |
| IL161988A0 (en) | 2001-11-16 | 2005-11-20 | Corporation Idec Pharmaceutica | Polycistronic expression of antibodies |
| USRE47770E1 (en) | 2002-07-18 | 2019-12-17 | Merus N.V. | Recombinant production of mixtures of antibodies |
| PT2314629E (pt) | 2002-07-18 | 2014-01-22 | Merus B V | Produção recombinante de misturas de anticorpos |
| EP1572994B1 (fr) | 2002-12-20 | 2007-02-21 | Chromagenics B.V. | Moyens et methodes pour la production d'une proteine par des ouvre-chromatine capable de rendre le chromatine plus accessible aux facteurs de transcription |
| US20100069614A1 (en) | 2008-06-27 | 2010-03-18 | Merus B.V. | Antibody producing non-human mammals |
| EP2395017A3 (fr) | 2003-05-30 | 2012-12-19 | Merus B.V. | Conception et utilisation de régions variables appariées de molécules de liaison spécifiques |
| ES2646560T3 (es) | 2004-01-20 | 2017-12-14 | Merus N.V. | Mezclas de proteínas de unión |
| US20060195935A1 (en) | 2004-11-08 | 2006-08-31 | Chromagenics B.V. | Selection of host cells expressing protein at high levels |
| PL1809750T3 (pl) | 2004-11-08 | 2012-08-31 | Chromagenics Bv | Selekcja komórki gospodarza eksprymującej białko na wysokich poziomach |
| US8999667B2 (en) | 2004-11-08 | 2015-04-07 | Chromagenics B.V. | Selection of host cells expressing protein at high levels |
| ES2384391T3 (es) | 2004-11-08 | 2012-07-04 | Chromagenics B.V. | Selección de células hospedantes que expresan proteína a altos niveles |
| US8039230B2 (en) | 2004-11-08 | 2011-10-18 | Chromagenics B.V. | Selection of host cells expressing protein at high levels |
| US9096878B2 (en) * | 2008-12-22 | 2015-08-04 | National University Corporation Hokkaido University | Expression vector for producing protein derived from foreign gene in large quantity using animal cells, and use thereof |
| SG173456A1 (en) | 2009-02-27 | 2011-09-29 | Novartis Ag | Expression vector system comprising two selection markers |
| MX360109B (es) | 2012-04-20 | 2018-10-23 | Merus Nv | Metodos y medios para la produccion de moleculas de tipo ig. |
| ES2689136T3 (es) | 2013-07-31 | 2018-11-08 | Novartis Ag | Nuevos vectores de selección y métodos de selección de células hospedadoras eucariotas |
| JP6709156B2 (ja) | 2013-12-20 | 2020-06-10 | ノバルティス アーゲー | 新規真核細胞および目的の生成物を組換え発現させるための方法 |
| ES2758504T3 (es) | 2013-12-20 | 2020-05-05 | Novartis Ag | Células eucariotas novedosas y métodos para expresar de forma recombinante un producto de interés |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2353384A (en) * | 1983-01-19 | 1984-07-26 | Genentech Inc. | Amplification in eukaryotic host cells |
| US5043270A (en) * | 1989-03-31 | 1991-08-27 | The Board Of Trustees Of The Leland Stanford Junior University | Intronic overexpression vectors |
-
1992
- 1992-02-20 AU AU15806/92A patent/AU660671B2/en not_active Expired
- 1992-02-20 CA CA002104598A patent/CA2104598C/fr not_active Expired - Lifetime
- 1992-02-20 DE DE69231676T patent/DE69231676T2/de not_active Expired - Lifetime
- 1992-02-20 WO PCT/US1992/001358 patent/WO1992017566A2/fr active IP Right Grant
- 1992-02-20 ES ES92908635T patent/ES2153358T3/es not_active Expired - Lifetime
- 1992-02-20 AT AT92908635T patent/ATE199023T1/de active
- 1992-02-20 JP JP50818192A patent/JP3438889B2/ja not_active Expired - Lifetime
- 1992-02-20 EP EP92908635A patent/EP0724639B1/fr not_active Expired - Lifetime
- 1992-02-20 DK DK92908635T patent/DK0724639T3/da active
-
2001
- 2001-03-20 GR GR20010400445T patent/GR3035599T3/el unknown
Also Published As
| Publication number | Publication date |
|---|---|
| JPH06506356A (ja) | 1994-07-21 |
| WO1992017566A2 (fr) | 1992-10-15 |
| WO1992017566A3 (fr) | 1993-01-21 |
| DE69231676T2 (de) | 2001-05-17 |
| EP0724639A1 (fr) | 1996-08-07 |
| DE69231676D1 (de) | 2001-03-08 |
| AU1580692A (en) | 1992-11-02 |
| EP0724639B1 (fr) | 2001-01-31 |
| AU660671B2 (en) | 1995-07-06 |
| ATE199023T1 (de) | 2001-02-15 |
| CA2104598A1 (fr) | 1992-09-30 |
| JP3438889B2 (ja) | 2003-08-18 |
| GR3035599T3 (en) | 2001-06-29 |
| ES2153358T3 (es) | 2001-03-01 |
| CA2104598C (fr) | 2003-01-07 |
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