DK0714988T3 - Fremgangsmåde til mængdebestemmelse af nukleinsyrer - Google Patents

Fremgangsmåde til mængdebestemmelse af nukleinsyrer

Info

Publication number
DK0714988T3
DK0714988T3 DK95890172T DK95890172T DK0714988T3 DK 0714988 T3 DK0714988 T3 DK 0714988T3 DK 95890172 T DK95890172 T DK 95890172T DK 95890172 T DK95890172 T DK 95890172T DK 0714988 T3 DK0714988 T3 DK 0714988T3
Authority
DK
Denmark
Prior art keywords
target
quantification
contg
less
nucleic acids
Prior art date
Application number
DK95890172T
Other languages
English (en)
Inventor
Friedrich Prof Dr Dorner
Thomas Dr Haemmerle
Falko-Guenter Dr Falkner
Johann Dr Kohl
Michele Dr Himmelspach
Original Assignee
Baxter Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from AT183194A external-priority patent/AT401062B/de
Priority claimed from AT224594A external-priority patent/ATA224594A/de
Application filed by Baxter Ag filed Critical Baxter Ag
Application granted granted Critical
Publication of DK0714988T3 publication Critical patent/DK0714988T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
DK95890172T 1994-09-26 1995-09-26 Fremgangsmåde til mængdebestemmelse af nukleinsyrer DK0714988T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
AT183194A AT401062B (de) 1994-09-26 1994-09-26 Verfahren zur quantifizierung von nukleinsäuren
AT224594A ATA224594A (de) 1994-12-02 1994-12-02 Verfahren zur quantifizierung von nukleinsäuren

Publications (1)

Publication Number Publication Date
DK0714988T3 true DK0714988T3 (da) 2002-04-08

Family

ID=25596921

Family Applications (1)

Application Number Title Priority Date Filing Date
DK95890172T DK0714988T3 (da) 1994-09-26 1995-09-26 Fremgangsmåde til mængdebestemmelse af nukleinsyrer

Country Status (8)

Country Link
US (1) US5789153A (da)
EP (1) EP0714988B1 (da)
JP (1) JPH08107798A (da)
AT (1) ATE210735T1 (da)
CA (1) CA2159044A1 (da)
DE (1) DE59509938D1 (da)
DK (1) DK0714988T3 (da)
ES (1) ES2169753T3 (da)

Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5814442A (en) * 1994-06-10 1998-09-29 Georgetown University Internally controlled virion nucleic acid amplification reaction for quantitation of virion and virion nucleic acid
AT406019B (de) * 1995-05-08 2000-01-25 Immuno Ag Verfahren zur herstellung eines arzneimittels enthaltend ein oder mehrere plasmaderivate
GB9519638D0 (en) * 1995-09-26 1995-11-29 Dynal As Method
US6265152B1 (en) * 1995-12-22 2001-07-24 Visible Genetics Inc. Method and kit for evaluation of HIV mutations
AT406778B (de) * 1996-04-09 2000-09-25 Immuno Ag Verfahren zur desintegration von nucleinsäuren und herstellung von qualitätsgesicherten biologischen produkten
AT405403B (de) 1997-02-27 1999-08-25 Immuno Ag Reinigung von von willebrand-faktor durch kationenaustauscherchromatographie
AT406373B (de) 1997-02-27 2000-04-25 Immuno Ag Verfahren zur reinigung von faktor viii/vwf-komplex mittels kationenaustauscherchromatographie
DE19752898A1 (de) * 1997-11-28 1999-08-05 Centeon Pharma Gmbh Verfahren zum Nachweis hoher Vierenkonzentrationen im Blutplasma und/oder Blutserum mittels der Polymerasekettenreaktion
AT407160B (de) * 1998-06-04 2001-01-25 Immuno Ag Verfahren zur bestimmung von antigenen
US6455692B1 (en) 1998-08-04 2002-09-24 Transgenomic, Inc. Method of concentrating polynucleotides using MIPC
GB9902422D0 (en) * 1999-02-03 1999-03-24 Lgc Teddington Limited Reference material for nucleic acid amplification
AT410218B (de) 1999-08-20 2003-03-25 Baxter Ag Verfahren zur herstellung eines qualitätsgesicherten pools biologischer proben
US6379889B1 (en) * 1999-11-04 2002-04-30 Agilent Technologies, Inc. Multiplexing methods for identifying nucleic acids using denaturing liquid chromatography
AT409382B (de) 1999-12-22 2002-07-25 Baxter Ag Verfahren zur detektion, unterscheidung und quantifizierung von freien und eingekapselten nukleinsäuren in einer probe
JP3752488B2 (ja) 2000-09-22 2006-03-08 株式会社日立製作所 核酸解析方法
US6312929B1 (en) * 2000-12-22 2001-11-06 Cepheid Compositions and methods enabling a totally internally controlled amplification reaction
US6982029B2 (en) * 2001-05-07 2006-01-03 Spectramedix Llc Electrophoretic method and system having internal lane standards for color calibration
US20030207310A1 (en) * 2002-05-01 2003-11-06 Zhaowei Liu System and method for determining the sizes and quantity of polynucleotides with capillary array electrophoresis
EP1706836B1 (en) * 2003-12-06 2011-01-19 Abbott Laboratories Method and system for analyzing reactions using an information system
US7544792B2 (en) 2004-07-13 2009-06-09 Gen-Probe Incorporated Compositions and methods for detection of hepatitis A virus nucleic acid
US7964413B2 (en) 2005-03-10 2011-06-21 Gen-Probe Incorporated Method for continuous mode processing of multiple reaction receptacles in a real-time amplification assay
JP5564795B2 (ja) * 2009-01-06 2014-08-06 株式会社島津製作所 Ｄｎａ定量方法、及び遺伝子解析方法
JP6121591B2 (ja) * 2010-07-29 2017-04-26 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 微生物核酸の定性的および定量的検出
CA2802548C (en) * 2010-07-29 2017-01-03 F. Hoffmann-La Roche Ag Qualitative and quantitative detection of microbial nucleic acids
US8877464B2 (en) 2010-07-29 2014-11-04 Roche Molecular Systems, Inc. Qualitative and quantitative detection of microbial nucleic acids
EP2627787B1 (en) 2010-10-14 2017-04-19 Rheonix, Inc. Quantitative multiplexed identification of nucleic acid targets
WO2012095748A2 (en) * 2011-01-14 2012-07-19 Smiths Detection - Watford Limited Single tube quantitative polymerase chain reaction (pcr)
EP2877952B1 (en) 2012-07-27 2023-04-12 Gen-Probe Incorporated Dual reference calibration method and system for quantifying polynucleotides
DE102018213027A1 (de) * 2018-08-03 2020-02-06 Robert Bosch Gmbh Reaktionsgemisch, Verfahren und Kit zur Durchführung einer quantitativen Echtzeit-PCR

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US6538126B1 (en) * 1991-06-10 2003-03-25 Lucky Limited Hepatitis C diagnostics and vaccines
JP2739004B2 (ja) * 1992-01-16 1998-04-08 三菱電機株式会社 半導体装置
AU4248193A (en) * 1992-05-15 1993-12-13 New England Deaconess Hospital Quantitation of viral rna by competitive polymerase chain reaction
WO1994010342A1 (en) * 1992-10-30 1994-05-11 The Rogosin Institute Method for quantifying nucleic acids
AU6359794A (en) * 1993-03-05 1994-09-26 Genelabs Technologies, Inc. Method for hiv quantitation
KR100312800B1 (ko) * 1993-07-09 2002-02-28 에프.지.엠. 헤르만스 ; 이.에이치. 리링크 핵산의정량방법
US5523204A (en) * 1993-12-10 1996-06-04 Becton Dickinson And Company Detection of nucleic acids in cells by strand displacement amplification
US5491063A (en) * 1994-09-01 1996-02-13 Hoffmann-La Roche Inc. Methods for in-solution quenching of fluorescently labeled oligonucleotide probes

Also Published As

Publication number Publication date
EP0714988B1 (de) 2001-12-12
ES2169753T3 (es) 2002-07-16
US5789153A (en) 1998-08-04
CA2159044A1 (en) 1996-03-27
EP0714988A2 (de) 1996-06-05
DE59509938D1 (de) 2002-01-24
EP0714988A3 (de) 1997-04-16
JPH08107798A (ja) 1996-04-30
ATE210735T1 (de) 2001-12-15

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