DK0510693T3 - Ekspression af humant serumalbumin i Pichia pastoris - Google Patents

Ekspression af humant serumalbumin i Pichia pastoris

Info

Publication number
DK0510693T3
DK0510693T3 DK92107062T DK92107062T DK0510693T3 DK 0510693 T3 DK0510693 T3 DK 0510693T3 DK 92107062 T DK92107062 T DK 92107062T DK 92107062 T DK92107062 T DK 92107062T DK 0510693 T3 DK0510693 T3 DK 0510693T3
Authority
DK
Denmark
Prior art keywords
pichia pastoris
hsa
regulatory region
structural gene
expression
Prior art date
Application number
DK92107062T
Other languages
Danish (da)
English (en)
Inventor
Kotikanyad Sreekrishna
Kathryn Ann Barr
Russell A Brierley
Gregory P Thill
Juerg F Tschopp
Original Assignee
Res Corp Technologies Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Res Corp Technologies Inc filed Critical Res Corp Technologies Inc
Application granted granted Critical
Publication of DK0510693T3 publication Critical patent/DK0510693T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/76—Albumins
    • C07K14/765—Serum albumin, e.g. HSA

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
DK92107062T 1991-04-25 1992-04-24 Ekspression af humant serumalbumin i Pichia pastoris DK0510693T3 (da)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US69160491A 1991-04-25 1991-04-25

Publications (1)

Publication Number Publication Date
DK0510693T3 true DK0510693T3 (da) 1999-09-27

Family

ID=24777211

Family Applications (1)

Application Number Title Priority Date Filing Date
DK92107062T DK0510693T3 (da) 1991-04-25 1992-04-24 Ekspression af humant serumalbumin i Pichia pastoris

Country Status (8)

Country Link
US (1) US5707828A (fr)
EP (1) EP0510693B1 (fr)
JP (2) JP3583791B2 (fr)
AT (1) ATE177149T1 (fr)
CA (1) CA2058820C (fr)
DE (1) DE69228497T2 (fr)
DK (1) DK0510693T3 (fr)
ES (1) ES2127734T3 (fr)

Families Citing this family (49)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5330901A (en) * 1991-04-26 1994-07-19 Research Corporation Technologies, Inc. Expression of human serum albumin in Pichia pastoris
FR2686899B1 (fr) 1992-01-31 1995-09-01 Rhone Poulenc Rorer Sa Nouveaux polypeptides biologiquement actifs, leur preparation et compositions pharmaceutiques les contenant.
WO1994020631A2 (fr) * 1993-03-03 1994-09-15 E.I. Du Pont De Nemours And Company Production de glycolate oxydase dans de la levure methylotrophique
GB9526733D0 (en) 1995-12-30 1996-02-28 Delta Biotechnology Ltd Fusion proteins
JP3794748B2 (ja) * 1996-03-04 2006-07-12 第一アスビオファーマ株式会社 メタノール代謝系を有する微生物の培養法
MX9605082A (es) 1996-10-24 1998-04-30 Univ Autonoma De Nuevo Leon Levaduras metilotroficas modificadas geneticamente para la produccion y secrecion de hormona de crecimiento humano.
CN1105727C (zh) * 1998-06-17 2003-04-16 上海海济医药生物工程有限公司 重组人血清白蛋白的生产方法
CN1103374C (zh) * 1999-03-04 2003-03-19 上海贸基生物工程科技有限公司 重组dna技术生产人血清白蛋白的方法
CN1103373C (zh) * 1999-03-04 2003-03-19 上海贸基生物工程科技有限公司 人血清白蛋白改构基因、表达载体及其宿主
AU3910000A (en) * 1999-03-22 2000-10-09 Zymogenetics Inc. Improved methods for producing proteins in transformed (pichia)
US6258559B1 (en) 1999-03-22 2001-07-10 Zymogenetics, Inc. Method for producing proteins in transformed Pichia
US6451564B1 (en) 1999-07-02 2002-09-17 Massachusetts Institute Of Technology Methods for producing L-isoleucine
US6927046B1 (en) * 1999-12-30 2005-08-09 Archer-Daniels-Midland Company Increased lysine production by gene amplification using coryneform bacteria
US6946134B1 (en) 2000-04-12 2005-09-20 Human Genome Sciences, Inc. Albumin fusion proteins
AU2001259063A1 (en) 2000-04-12 2001-10-30 Human Genome Sciences, Inc. Albumin fusion proteins
US7029872B2 (en) 2000-06-28 2006-04-18 Glycofi, Inc Methods for producing modified glycoproteins
US7449308B2 (en) * 2000-06-28 2008-11-11 Glycofi, Inc. Combinatorial DNA library for producing modified N-glycans in lower eukaryotes
US7863020B2 (en) 2000-06-28 2011-01-04 Glycofi, Inc. Production of sialylated N-glycans in lower eukaryotes
US7598055B2 (en) * 2000-06-28 2009-10-06 Glycofi, Inc. N-acetylglucosaminyltransferase III expression in lower eukaryotes
US7625756B2 (en) 2000-06-28 2009-12-01 GycoFi, Inc. Expression of class 2 mannosidase and class III mannosidase in lower eukaryotic cells
US8697394B2 (en) * 2000-06-28 2014-04-15 Glycofi, Inc. Production of modified glycoproteins having multiple antennary structures
MXPA03009059A (es) * 2001-04-05 2004-10-15 Univ Nebraska Secuencias de nucleotidos reguladoras de alcohol oxidasa 1 para expresion de genes heterologos en levadura.
US6645739B2 (en) * 2001-07-26 2003-11-11 Phoenix Pharmacologies, Inc. Yeast expression systems, methods of producing polypeptides in yeast, and compositions relating to same
US6890722B2 (en) * 2001-11-23 2005-05-10 Syn X Pharma, Inc. HP biopolymer markers predictive of insulin resistance
EP1463751B1 (fr) 2001-12-21 2013-05-22 Human Genome Sciences, Inc. Proteines hybrides d'albumine
EP1433842B1 (fr) * 2002-12-18 2011-01-05 Roche Diagnostics GmbH Désoxyribonucléase I recombinante issue de pancréas bovine à haute activité spécifique
DE60335602D1 (de) * 2002-12-18 2011-02-17 Roche Diagnostics Gmbh Rekombinante Desoxyribonuclease I aus Rinder-Pankreas mit hoher spezifischer Aktivität
US7332299B2 (en) 2003-02-20 2008-02-19 Glycofi, Inc. Endomannosidases in the modification of glycoproteins in eukaryotes
WO2004108890A2 (fr) * 2003-05-30 2004-12-16 New Century Pharmaceuticals, Inc. Production de proteines et de polypeptides de recombinaison au moyen de micro-organismes methylotropes ou ethylotropes avec une alimentation en methanol ou en ethanol dilue
AT501955B1 (de) * 2005-02-23 2007-08-15 Univ Graz Tech Mutierte aox1-promotoren
EP2069396B1 (fr) 2006-09-08 2015-10-21 Ambrx, Inc. Polypeptide plasmatique humain modifie ou squelettes de fc et leurs utilisations
EP2300615A2 (fr) * 2008-07-11 2011-03-30 Novozymes A/S Variante du promoteur das de pichia pastoris
US9493545B2 (en) 2009-02-11 2016-11-15 Albumedix A/S Albumin variants and conjugates
US8748380B2 (en) 2009-10-30 2014-06-10 Novozymes Biopharma Dk A/S Albumin variants
US10233228B2 (en) 2010-04-09 2019-03-19 Albumedix Ltd Albumin derivatives and variants
EP2705051A1 (fr) 2011-05-05 2014-03-12 Novozymes Biopharma DK A/S Variants de l'albumine
US20140315817A1 (en) 2011-11-18 2014-10-23 Eleven Biotherapeutics, Inc. Variant serum albumin with improved half-life and other properties
AU2013234299B2 (en) 2012-03-16 2017-06-22 Albumedix Ltd. Albumin variants
MX2015005363A (es) 2012-11-08 2015-11-06 Novozymes Biopharma Dk As Variantes de albumina.
WO2014096440A2 (fr) 2012-12-21 2014-06-26 Novozymes Biopharma Dk A/S Composition
CN104854245B (zh) 2012-12-21 2020-02-14 刘平华 通过代谢工程生产麦角硫因
WO2014160871A2 (fr) 2013-03-27 2014-10-02 The General Hospital Corporation Procédés et agents pour le traitement de la maladie d'alzheimer
WO2015095604A2 (fr) 2013-12-18 2015-06-25 President And Fellows Of Harvard College Méthodes et analyses associées à des cellules tumorales circulantes
CN108289928A (zh) 2015-08-06 2018-07-17 哈佛大学校长及研究员协会 改进的微生物-结合分子和其用途
CA2989966C (fr) 2015-08-20 2024-04-30 Albumedix A/S Variants de l'albumine et leurs conjugues
US11345891B2 (en) 2017-04-05 2022-05-31 Asgard Therapeutics Ab Compositions for reprogramming cells into dendritic cells or antigen presenting cells, methods and uses thereof
EP3385373A1 (fr) 2017-04-05 2018-10-10 Centro de Neurociências e Biologia Celular Compositions pour la reprogrammation de cellules en cellules dendritiques ou en cellules présentatrices d'antigènes, leurs procédés et utilisations
CN113056554A (zh) * 2018-11-19 2021-06-29 巴斯夫欧洲公司 重组酵母细胞
US20240115607A1 (en) 2021-01-26 2024-04-11 Cytocares (Shanghai) Inc. Chimeric antigen receptor (car) constructs and nk cells expressing car constructs

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL66614A (en) * 1981-08-28 1985-09-29 Genentech Inc Method of constructing a dna sequence encoding a polypeptide,microbial production of human serum albumin,and pharmaceutical compositions comprising it
US4775622A (en) * 1982-03-08 1988-10-04 Genentech, Inc. Expression, processing and secretion of heterologous protein by yeast
US4885242A (en) * 1984-10-30 1989-12-05 Phillips Petroleum Company Genes from pichia histidine pathway and uses thereof
US4808537A (en) * 1984-10-30 1989-02-28 Phillips Petroleum Company Methanol inducible genes obtained from pichia and methods of use
US4837148A (en) * 1984-10-30 1989-06-06 Phillips Petroleum Company Autonomous replication sequences for yeast strains of the genus pichia
US4855231A (en) * 1984-10-30 1989-08-08 Phillips Petroleum Company Regulatory region for heterologous gene expression in yeast
KR870000428A (ko) * 1985-06-17 1987-02-18 . 인체혈청 알부민의 제조방법
US4882279A (en) * 1985-10-25 1989-11-21 Phillips Petroleum Company Site selective genomic modification of yeast of the genus pichia
US4895800A (en) * 1985-11-26 1990-01-23 Phillips Petroleum Company Yeast production of hepatitis B surface antigen
GB8613388D0 (en) * 1986-06-03 1986-07-09 Delta Biotechnology Ltd Induction of galactose regulated gene expression in yeast
GB8615701D0 (en) * 1986-06-27 1986-08-06 Delta Biotechnology Ltd Stable gene integration vector
IL82935A0 (en) * 1986-08-12 1987-12-20 Phillips Petroleum Co Secretion of heterologous proteins from yeast
IL89992A0 (en) * 1988-04-25 1989-12-15 Phillips Petroleum Co Expression of human serum albumin in methylotrophic yeasts

Also Published As

Publication number Publication date
CA2058820A1 (fr) 1992-10-26
DE69228497T2 (de) 1999-07-15
ES2127734T3 (es) 1999-05-01
EP0510693B1 (fr) 1999-03-03
EP0510693A2 (fr) 1992-10-28
ATE177149T1 (de) 1999-03-15
CA2058820C (fr) 2003-07-15
JP3786662B2 (ja) 2006-06-14
JP3583791B2 (ja) 2004-11-04
JPH0646858A (ja) 1994-02-22
JP2004166711A (ja) 2004-06-17
EP0510693A3 (fr) 1994-01-12
US5707828A (en) 1998-01-13
DE69228497D1 (de) 1999-04-08

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