DE69228497D1 - Expression von menschlichen Serum-Albuminen in Pichia Pastoris - Google Patents

Expression von menschlichen Serum-Albuminen in Pichia Pastoris

Info

Publication number
DE69228497D1
DE69228497D1 DE69228497T DE69228497T DE69228497D1 DE 69228497 D1 DE69228497 D1 DE 69228497D1 DE 69228497 T DE69228497 T DE 69228497T DE 69228497 T DE69228497 T DE 69228497T DE 69228497 D1 DE69228497 D1 DE 69228497D1
Authority
DE
Germany
Prior art keywords
pichia pastoris
hsa
regulatory region
structural gene
expression
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69228497T
Other languages
English (en)
Other versions
DE69228497T2 (de
Inventor
Kotikanyad Sreekrishna
Kathryn Ann Barr
Russell A Brierley
Gregory P Thill
Juerg F Tschopp
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Research Corp Technologies Inc
Original Assignee
Research Corp Technologies Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Research Corp Technologies Inc filed Critical Research Corp Technologies Inc
Publication of DE69228497D1 publication Critical patent/DE69228497D1/de
Application granted granted Critical
Publication of DE69228497T2 publication Critical patent/DE69228497T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/76—Albumins
    • C07K14/765—Serum albumin, e.g. HSA

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
DE69228497T 1991-04-25 1992-04-24 Expression von menschlichen Serum-Albuminen in Pichia Pastoris Expired - Lifetime DE69228497T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US69160491A 1991-04-25 1991-04-25

Publications (2)

Publication Number Publication Date
DE69228497D1 true DE69228497D1 (de) 1999-04-08
DE69228497T2 DE69228497T2 (de) 1999-07-15

Family

ID=24777211

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69228497T Expired - Lifetime DE69228497T2 (de) 1991-04-25 1992-04-24 Expression von menschlichen Serum-Albuminen in Pichia Pastoris

Country Status (8)

Country Link
US (1) US5707828A (de)
EP (1) EP0510693B1 (de)
JP (2) JP3583791B2 (de)
AT (1) ATE177149T1 (de)
CA (1) CA2058820C (de)
DE (1) DE69228497T2 (de)
DK (1) DK0510693T3 (de)
ES (1) ES2127734T3 (de)

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MX9605082A (es) 1996-10-24 1998-04-30 Univ Autonoma De Nuevo Leon Levaduras metilotroficas modificadas geneticamente para la produccion y secrecion de hormona de crecimiento humano.
CN1105727C (zh) * 1998-06-17 2003-04-16 上海海济医药生物工程有限公司 重组人血清白蛋白的生产方法
CN1103373C (zh) * 1999-03-04 2003-03-19 上海贸基生物工程科技有限公司 人血清白蛋白改构基因、表达载体及其宿主
CN1103374C (zh) * 1999-03-04 2003-03-19 上海贸基生物工程科技有限公司 重组dna技术生产人血清白蛋白的方法
US20040010134A1 (en) 2000-04-12 2004-01-15 Rosen Craig A. Albumin fusion proteins
US6258559B1 (en) 1999-03-22 2001-07-10 Zymogenetics, Inc. Method for producing proteins in transformed Pichia
WO2000056903A2 (en) * 1999-03-22 2000-09-28 Zymogenetics, Inc. IMPROVED METHODS FOR PRODUCING PROTEINS IN TRANSFORMED $i(PICHIA)
US6451564B1 (en) * 1999-07-02 2002-09-17 Massachusetts Institute Of Technology Methods for producing L-isoleucine
US6927046B1 (en) 1999-12-30 2005-08-09 Archer-Daniels-Midland Company Increased lysine production by gene amplification using coryneform bacteria
US6946134B1 (en) 2000-04-12 2005-09-20 Human Genome Sciences, Inc. Albumin fusion proteins
US7598055B2 (en) * 2000-06-28 2009-10-06 Glycofi, Inc. N-acetylglucosaminyltransferase III expression in lower eukaryotes
US7863020B2 (en) 2000-06-28 2011-01-04 Glycofi, Inc. Production of sialylated N-glycans in lower eukaryotes
EP2339013B1 (de) 2000-06-28 2014-07-02 GlycoFi, Inc. Expression von Klasse-2-Mannosidase und Klasse-III-Mannosidase in niederen eukaryotischen Zellen
US8697394B2 (en) * 2000-06-28 2014-04-15 Glycofi, Inc. Production of modified glycoproteins having multiple antennary structures
US7625756B2 (en) 2000-06-28 2009-12-01 GycoFi, Inc. Expression of class 2 mannosidase and class III mannosidase in lower eukaryotic cells
US7449308B2 (en) * 2000-06-28 2008-11-11 Glycofi, Inc. Combinatorial DNA library for producing modified N-glycans in lower eukaryotes
CA2442935A1 (en) * 2001-04-05 2002-10-17 The Board Of Regents Of The University Of Nebraska Alcohol oxidase 1 regulatory nucleotide sequences for heterologous gene expression in yeast
US6645739B2 (en) * 2001-07-26 2003-11-11 Phoenix Pharmacologies, Inc. Yeast expression systems, methods of producing polypeptides in yeast, and compositions relating to same
US6890722B2 (en) * 2001-11-23 2005-05-10 Syn X Pharma, Inc. HP biopolymer markers predictive of insulin resistance
DK1463751T3 (da) 2001-12-21 2013-08-26 Human Genome Sciences Inc Albuminfusionsproteiner.
EP1433842B1 (de) * 2002-12-18 2011-01-05 Roche Diagnostics GmbH Rekombinante Desoxyribonuclease I aus Rinder-Pankreas mit hoher spezifischer Aktivität
DE60335602D1 (de) * 2002-12-18 2011-02-17 Roche Diagnostics Gmbh Rekombinante Desoxyribonuclease I aus Rinder-Pankreas mit hoher spezifischer Aktivität
US7332299B2 (en) 2003-02-20 2008-02-19 Glycofi, Inc. Endomannosidases in the modification of glycoproteins in eukaryotes
WO2004108890A2 (en) * 2003-05-30 2004-12-16 New Century Pharmaceuticals, Inc. Dilute methanol -or ethanol- induced production of polypeptides in microorganisms
AT501955B1 (de) * 2005-02-23 2007-08-15 Univ Graz Tech Mutierte aox1-promotoren
AU2007292903B2 (en) 2006-09-08 2012-03-29 Ambrx, Inc. Modified human plasma polypeptide or Fc scaffolds and their uses
EP2300615A2 (de) * 2008-07-11 2011-03-30 Novozymes A/S Das-promotervarianten aus pichia pastoris
CN106986933A (zh) 2009-02-11 2017-07-28 阿尔布梅迪克斯医疗公司 白蛋白变体和缀合物
RU2607374C2 (ru) 2009-10-30 2017-01-10 Новозаймс Байофарма Дк А/С Варианты альбумина
KR20130070576A (ko) 2010-04-09 2013-06-27 노보자임스 바이오파마 디케이 에이/에스 알부민 유도체 및 변이체
CN104011072B (zh) 2011-05-05 2018-10-12 阿尔布梅迪克斯医疗有限公司 白蛋白变体
US20140315817A1 (en) 2011-11-18 2014-10-23 Eleven Biotherapeutics, Inc. Variant serum albumin with improved half-life and other properties
CA2861592A1 (en) 2012-03-16 2013-09-19 Novozymes Biopharma Dk A/S Albumin variants
WO2014072481A1 (en) 2012-11-08 2014-05-15 Novozymes Biopharma Dk A/S Albumin variants
EP2935603A4 (de) 2012-12-21 2016-07-13 Pinghua Liu Herstellung von ergothionein durch metabolische manipulation
WO2014096440A2 (en) 2012-12-21 2014-06-26 Novozymes Biopharma Dk A/S Composition
EP2978446B1 (de) 2013-03-27 2020-03-04 The General Hospital Corporation Anti-cd33 antikörper zur behandlung von morbus alzheimer
US10513546B2 (en) 2013-12-18 2019-12-24 President And Fellows Of Harvard College CRP capture/detection of gram positive bacteria
US10435457B2 (en) 2015-08-06 2019-10-08 President And Fellows Of Harvard College Microbe-binding molecules and uses thereof
EP3337816B1 (de) 2015-08-20 2024-02-14 Albumedix Ltd Albumin-varianten und -konjugate
EP3385373A1 (de) 2017-04-05 2018-10-10 Centro de Neurociências e Biologia Celular Zusammensetzungen zur umprogrammierung von zellen in dendritischen zellen oder antigen-präsentierende zellen, verfahren und verwendungen davon
US11345891B2 (en) 2017-04-05 2022-05-31 Asgard Therapeutics Ab Compositions for reprogramming cells into dendritic cells or antigen presenting cells, methods and uses thereof
CN113056554A (zh) * 2018-11-19 2021-06-29 巴斯夫欧洲公司 重组酵母细胞
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US4885242A (en) * 1984-10-30 1989-12-05 Phillips Petroleum Company Genes from pichia histidine pathway and uses thereof
US4808537A (en) * 1984-10-30 1989-02-28 Phillips Petroleum Company Methanol inducible genes obtained from pichia and methods of use
US4837148A (en) * 1984-10-30 1989-06-06 Phillips Petroleum Company Autonomous replication sequences for yeast strains of the genus pichia
KR870000428A (ko) * 1985-06-17 1987-02-18 . 인체혈청 알부민의 제조방법
US4882279A (en) * 1985-10-25 1989-11-21 Phillips Petroleum Company Site selective genomic modification of yeast of the genus pichia
US4895800A (en) * 1985-11-26 1990-01-23 Phillips Petroleum Company Yeast production of hepatitis B surface antigen
GB8613388D0 (en) * 1986-06-03 1986-07-09 Delta Biotechnology Ltd Induction of galactose regulated gene expression in yeast
GB8615701D0 (en) * 1986-06-27 1986-08-06 Delta Biotechnology Ltd Stable gene integration vector
IL82935A0 (en) * 1986-08-12 1987-12-20 Phillips Petroleum Co Secretion of heterologous proteins from yeast
IL89992A0 (en) * 1988-04-25 1989-12-15 Phillips Petroleum Co Expression of human serum albumin in methylotrophic yeasts

Also Published As

Publication number Publication date
US5707828A (en) 1998-01-13
JP3583791B2 (ja) 2004-11-04
DK0510693T3 (da) 1999-09-27
CA2058820C (en) 2003-07-15
ES2127734T3 (es) 1999-05-01
EP0510693A3 (de) 1994-01-12
EP0510693A2 (de) 1992-10-28
CA2058820A1 (en) 1992-10-26
ATE177149T1 (de) 1999-03-15
JP2004166711A (ja) 2004-06-17
EP0510693B1 (de) 1999-03-03
JPH0646858A (ja) 1994-02-22
JP3786662B2 (ja) 2006-06-14
DE69228497T2 (de) 1999-07-15

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