DE865502T1 - Auf fluoreszenz basierendes erkennungsverfahren für liganden - Google Patents
Auf fluoreszenz basierendes erkennungsverfahren für ligandenInfo
- Publication number
- DE865502T1 DE865502T1 DE0865502T DE96944304T DE865502T1 DE 865502 T1 DE865502 T1 DE 865502T1 DE 0865502 T DE0865502 T DE 0865502T DE 96944304 T DE96944304 T DE 96944304T DE 865502 T1 DE865502 T1 DE 865502T1
- Authority
- DE
- Germany
- Prior art keywords
- test
- target protein
- ligand
- control compositions
- protein
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000003446 ligand Substances 0.000 title claims 10
- 238000001514 detection method Methods 0.000 title 1
- 102000004169 proteins and genes Human genes 0.000 claims 11
- 108090000623 proteins and genes Proteins 0.000 claims 11
- 239000000203 mixture Substances 0.000 claims 10
- 238000000034 method Methods 0.000 claims 9
- 239000007850 fluorescent dye Substances 0.000 claims 4
- MPPQGYCZBNURDG-UHFFFAOYSA-N 2-propionyl-6-dimethylaminonaphthalene Chemical compound C1=C(N(C)C)C=CC2=CC(C(=O)CC)=CC=C21 MPPQGYCZBNURDG-UHFFFAOYSA-N 0.000 claims 2
- FWEOQOXTVHGIFQ-UHFFFAOYSA-N 8-anilinonaphthalene-1-sulfonic acid Chemical compound C=12C(S(=O)(=O)O)=CC=CC2=CC=CC=1NC1=CC=CC=C1 FWEOQOXTVHGIFQ-UHFFFAOYSA-N 0.000 claims 2
- 239000000523 sample Substances 0.000 claims 2
- 150000001875 compounds Chemical class 0.000 claims 1
- 239000003398 denaturant Substances 0.000 claims 1
- 230000000368 destabilizing effect Effects 0.000 claims 1
- VWLWTJHKQHRTNC-UHFFFAOYSA-L dipotassium;8-anilino-5-(4-anilino-5-sulfonatonaphthalen-1-yl)naphthalene-1-sulfonate Chemical group [K+].[K+].C=12C(S(=O)(=O)[O-])=CC=CC2=C(C=2C3=CC=CC(=C3C(NC=3C=CC=CC=3)=CC=2)S([O-])(=O)=O)C=CC=1NC1=CC=CC=C1 VWLWTJHKQHRTNC-UHFFFAOYSA-L 0.000 claims 1
- 239000003814 drug Substances 0.000 claims 1
- 150000004676 glycans Chemical class 0.000 claims 1
- 150000002632 lipids Chemical class 0.000 claims 1
- 238000005259 measurement Methods 0.000 claims 1
- 229910052751 metal Inorganic materials 0.000 claims 1
- 239000002184 metal Substances 0.000 claims 1
- 150000002739 metals Chemical class 0.000 claims 1
- 230000035772 mutation Effects 0.000 claims 1
- 102000039446 nucleic acids Human genes 0.000 claims 1
- 108020004707 nucleic acids Proteins 0.000 claims 1
- 150000007523 nucleic acids Chemical class 0.000 claims 1
- 229920001282 polysaccharide Polymers 0.000 claims 1
- 239000005017 polysaccharide Substances 0.000 claims 1
- 102000004196 processed proteins & peptides Human genes 0.000 claims 1
- 108090000765 processed proteins & peptides Proteins 0.000 claims 1
- 238000012216 screening Methods 0.000 claims 1
- 230000000087 stabilizing effect Effects 0.000 claims 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Organic Chemistry (AREA)
- Immunology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Cell Biology (AREA)
- Pathology (AREA)
- Biophysics (AREA)
- General Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Peptides Or Proteins (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US834695P | 1995-12-07 | 1995-12-07 | |
| PCT/US1996/019698 WO1997020952A1 (fr) | 1995-12-07 | 1996-12-06 | Procede de criblage par fluorescence destine a l'identification de ligands |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE865502T1 true DE865502T1 (de) | 1999-08-19 |
Family
ID=21731102
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE0865502T Pending DE865502T1 (de) | 1995-12-07 | 1996-12-06 | Auf fluoreszenz basierendes erkennungsverfahren für liganden |
Country Status (5)
| Country | Link |
|---|---|
| EP (1) | EP0865502A4 (fr) |
| JP (1) | JP2000502440A (fr) |
| CA (1) | CA2236339A1 (fr) |
| DE (1) | DE865502T1 (fr) |
| WO (1) | WO1997020952A1 (fr) |
Families Citing this family (24)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2184195C (fr) | 1995-10-25 | 2002-04-16 | Andrew Pakula | Methode d'analyse pour la caracterisation de ligands de proteines cibles |
| AU741049B2 (en) | 1996-05-09 | 2001-11-22 | Life Technologies Corporation | Microplate thermal shift assay and apparatus for ligand development and multi-variable protein chemistry optimization |
| US6571227B1 (en) | 1996-11-04 | 2003-05-27 | 3-Dimensional Pharmaceuticals, Inc. | Method, system and computer program product for non-linear mapping of multi-dimensional data |
| JP2001507675A (ja) | 1996-11-04 | 2001-06-12 | ３―ディメンショナル ファーマシューティカルズ インコーポレイテッド | 所望の特性を有する化合物を識別するシステム、方法、コンピュータ・プログラム製品 |
| US6453246B1 (en) | 1996-11-04 | 2002-09-17 | 3-Dimensional Pharmaceuticals, Inc. | System, method, and computer program product for representing proximity data in a multi-dimensional space |
| US6261842B1 (en) | 1997-10-23 | 2001-07-17 | Wisconsin Alumni Research Foundation | Microorganism genomics, compositions and methods related thereto |
| HU225767B1 (en) * | 1997-11-12 | 2007-08-28 | Dimensional Pharmaceuticals 3 | High throughput method for functionally classifying proteins identified using a genomics approach |
| US20010041964A1 (en) | 1998-09-14 | 2001-11-15 | George M. Grass | Pharmacokinetic-based drug design tool and method |
| US6569631B1 (en) | 1998-11-12 | 2003-05-27 | 3-Dimensional Pharmaceuticals, Inc. | Microplate thermal shift assay for ligand development using 5-(4″dimethylaminophenyl)-2-(4′-phenyl)oxazole derivative fluorescent dyes |
| US6358708B1 (en) | 1999-02-04 | 2002-03-19 | Anadys Pharmaceuticals, Inc. | Candida albicans SRB-7 |
| US6972198B2 (en) | 1999-02-26 | 2005-12-06 | Cyclacel, Ltd. | Methods and compositions using protein binding partners |
| US6582907B1 (en) * | 1999-12-09 | 2003-06-24 | Pharmacia & Upjohn Company | Use of fluorescence correlation spectroscopy to identify compounds that bind to target species under isothermal denaturing conditions |
| US6376180B1 (en) | 1999-12-09 | 2002-04-23 | Pharmacia & Upjohn Company | Methods of identifying compounds that bind to target species under isothermal denaturing conditions |
| US7416524B1 (en) | 2000-02-18 | 2008-08-26 | Johnson & Johnson Pharmaceutical Research & Development, L.L.C. | System, method and computer program product for fast and efficient searching of large chemical libraries |
| WO2001065462A2 (fr) | 2000-02-29 | 2001-09-07 | 3-Dimensional Pharmaceuticals, Inc. | Procede et produit de programme informatique de conception de tableaux combinatoires |
| WO2001071624A1 (fr) | 2000-03-22 | 2001-09-27 | 3-Dimensional Pharmaceuticals, Inc. | Systeme, procede et progiciel pour la representation de relations entre objets dans un espace multidimensionnel |
| AU2001249805A1 (en) | 2000-04-03 | 2001-10-15 | 3-Dimensional Pharmaceuticals, Inc. | Method, system, and computer program product for representing object relationships in a multidimensional space |
| US7054757B2 (en) | 2001-01-29 | 2006-05-30 | Johnson & Johnson Pharmaceutical Research & Development, L.L.C. | Method, system, and computer program product for analyzing combinatorial libraries |
| US20030059811A1 (en) * | 2001-06-14 | 2003-03-27 | Hakim Djaballah | Methods of screening for ligands of target molecules |
| EP1637885A1 (fr) | 2004-09-16 | 2006-03-22 | Vivalis | Méthode de criblage en employant des peptides sensibles à la conformation de protéines |
| GB0421285D0 (en) | 2004-09-24 | 2004-10-27 | Univ Nottingham | Improvements in high content screening |
| US8158340B2 (en) | 2005-12-28 | 2012-04-17 | Corning Incorporated | Methods for detecting conformational changes in bioentities |
| WO2016127100A1 (fr) | 2015-02-05 | 2016-08-11 | Promega Corporation | Analyses à variation thermique fondées sur la luciférase |
| GB202118173D0 (en) * | 2021-12-15 | 2022-01-26 | Medicines Discovery Catpult Ltd | Protein stability assay |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5231755B2 (fr) * | 1973-01-08 | 1977-08-17 | ||
| US5200504A (en) * | 1990-10-02 | 1993-04-06 | The Scripps Research Institute | Metallopeptides having stabilized secondary structures |
| CA2115900A1 (fr) * | 1993-02-22 | 1994-08-23 | Gerald W. Becker | Cribles pharmaceutiques et anticorps |
-
1996
- 1996-12-06 JP JP9521492A patent/JP2000502440A/ja active Pending
- 1996-12-06 DE DE0865502T patent/DE865502T1/de active Pending
- 1996-12-06 CA CA002236339A patent/CA2236339A1/fr not_active Abandoned
- 1996-12-06 WO PCT/US1996/019698 patent/WO1997020952A1/fr not_active Application Discontinuation
- 1996-12-06 EP EP96944304A patent/EP0865502A4/fr not_active Withdrawn
Also Published As
| Publication number | Publication date |
|---|---|
| EP0865502A4 (fr) | 2000-08-16 |
| EP0865502A1 (fr) | 1998-09-23 |
| CA2236339A1 (fr) | 1997-06-12 |
| WO1997020952A1 (fr) | 1997-06-12 |
| JP2000502440A (ja) | 2000-02-29 |
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