DE69942309D1 - In situ hybridisierungsverfahren zur entdeckung von zielnukleinsäuren - Google Patents

In situ hybridisierungsverfahren zur entdeckung von zielnukleinsäuren

Info

Publication number
DE69942309D1
DE69942309D1 DE69942309T DE69942309T DE69942309D1 DE 69942309 D1 DE69942309 D1 DE 69942309D1 DE 69942309 T DE69942309 T DE 69942309T DE 69942309 T DE69942309 T DE 69942309T DE 69942309 D1 DE69942309 D1 DE 69942309D1
Authority
DE
Germany
Prior art keywords
sample
target nucleic
probe complex
detecting
nucleic acids
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69942309T
Other languages
English (en)
Inventor
Jyotsna S Shah
Nick S Harris
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Igenex Inc
Original Assignee
Igenex Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Igenex Inc filed Critical Igenex Inc
Application granted granted Critical
Publication of DE69942309D1 publication Critical patent/DE69942309D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6893—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for protozoa
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
DE69942309T 1998-05-18 1999-05-18 In situ hybridisierungsverfahren zur entdeckung von zielnukleinsäuren Expired - Lifetime DE69942309D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US8856198P 1998-05-18 1998-05-18
PCT/US1999/011046 WO1999060163A1 (en) 1998-05-18 1999-05-18 In situ hybridization method for detecting target nucleic acid

Publications (1)

Publication Number Publication Date
DE69942309D1 true DE69942309D1 (de) 2010-06-10

Family

ID=22212089

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69942309T Expired - Lifetime DE69942309D1 (de) 1998-05-18 1999-05-18 In situ hybridisierungsverfahren zur entdeckung von zielnukleinsäuren

Country Status (11)

Country Link
US (1) US6165723A (de)
EP (1) EP1080228B1 (de)
AT (1) ATE466099T1 (de)
AU (1) AU4087399A (de)
CA (1) CA2332510C (de)
CY (1) CY1110164T1 (de)
DE (1) DE69942309D1 (de)
DK (1) DK1080228T3 (de)
ES (1) ES2344873T3 (de)
PT (1) PT1080228E (de)
WO (1) WO1999060163A1 (de)

Families Citing this family (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK1005633T3 (da) 1997-08-20 2008-12-15 Univ Miami Vævsfikserings-dehydratiserings-fedtfjernelses-imprægneringsmetode med höj kvalitet og kontinuerligt gennemlöb
US6329149B1 (en) * 2000-03-07 2001-12-11 Janice Cori Cobb Reverse-root-canal method for extracting aDNA
US20040115692A1 (en) * 2000-04-03 2004-06-17 Cytyc Corporation Methods, compositions and apparatuses for detecting a target in a preservative solution
US7138226B2 (en) * 2002-05-10 2006-11-21 The University Of Miami Preservation of RNA and morphology in cells and tissues
JP2004135509A (ja) * 2002-10-15 2004-05-13 Hitachi Ltd 遺伝子発現解析法及び遺伝子発現解析用プローブキット
US8062897B2 (en) 2003-07-21 2011-11-22 Aureon Laboratories, Inc. Diagnostic histopathology using multiplex gene expression FISH
DK1676117T3 (da) 2003-10-24 2009-11-09 Univ Miami Simplificeret forarbejdning af væv
EP1766061B1 (de) 2004-05-20 2013-07-17 Quest Diagnostics Investments Incorporated Vergleichende einfachmarkierung-hybridisierung
JP4424155B2 (ja) * 2004-10-27 2010-03-03 富士通株式会社 移動通信システム、移動局
US8021888B2 (en) 2005-01-27 2011-09-20 Quest Diagnostics Investments Incorporated Rapid comparative genomic hybridization using acoustic surface waves
US8758990B2 (en) * 2005-07-28 2014-06-24 Id-Fish Technology, Inc. Methods for detecting and differentiating Mycobacterium genus and Mycobacterium avium complex in a sample or culture
CA2641599C (en) * 2005-07-28 2012-07-10 Id-Fish Technology, Inc. Method for improving cell permeability to foreign particles
US8076074B2 (en) 2005-11-29 2011-12-13 Quest Diagnostics Investments Incorporated Balanced translocation in comparative hybridization
US8530196B2 (en) 2006-04-07 2013-09-10 The Trustees Of The Univeristy Of Pennsylvania In situ cloning from pathological tissue specimens
US8323901B2 (en) * 2006-11-30 2012-12-04 Id-Fish Technology, Inc. Nucleic acid probes and methods for detecting plasmodium parasites
BRPI0717707B8 (pt) 2006-11-30 2021-07-27 Id Fish Tech Inc método para detectar a presença de plasmodium em uma amostra, fragmento de ácido nucléico e método para detectar e diferenciar espécies de protozoários
US7807359B2 (en) 2006-12-01 2010-10-05 Quest Diagnostics Investments Incorporated Methods of detecting TPMT mutations
US7507539B2 (en) 2007-07-30 2009-03-24 Quest Diagnostics Investments Incorporated Substractive single label comparative hybridization
US8093063B2 (en) 2007-11-29 2012-01-10 Quest Diagnostics Investments Incorporated Assay for detecting genetic abnormalities in genomic nucleic acids
KR101020844B1 (ko) * 2008-09-04 2011-03-09 삼성전기주식회사 구리 나노입자의 저온 환원 소결을 위한 환원제 및 이를이용한 저온 소결 방법
US8039794B2 (en) 2008-12-16 2011-10-18 Quest Diagnostics Investments Incorporated Mass spectrometry assay for thiopurine-S-methyl transferase activity and products generated thereby
US20110086772A1 (en) 2009-09-25 2011-04-14 Signature Genomics Laboratories Llc Multiplex (+/-) stranded arrays and assays for detecting chromosomal abnormalities associated with cancer and other diseases
EP2563935B1 (de) 2010-04-30 2014-04-16 Exiqon A/S In-situ-hybridisierungsverfahren und puffer
US9944975B2 (en) 2015-09-03 2018-04-17 Abbott Molecular Inc. Hybridization buffers
EP3400307A1 (de) * 2016-01-08 2018-11-14 Abbott Molecular Inc. Hybridisierungspuffer mit guanidiniumthiocyanat
AU2018281196B2 (en) * 2017-06-07 2022-04-28 Gen-Probe Incorporated Detecting Babesia species nucleic acid in a sample

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5447841A (en) * 1986-01-16 1995-09-05 The Regents Of The Univ. Of California Methods for chromosome-specific staining
US4886741A (en) * 1987-12-09 1989-12-12 Microprobe Corporation Use of volume exclusion agents for the enhancement of in situ hybridization
JPH04502552A (ja) * 1988-08-31 1992-05-14 アプロジェネックス，インク ワン―ステップのインシチュハイブリッド形成分析法
US5264343A (en) * 1990-08-31 1993-11-23 Eleanor Roosevelt Institute Method for distinguishing normal and cancer cells
US5627029A (en) * 1991-05-03 1997-05-06 Genzyme Corporation Method for obtaining informative cells
US5506098A (en) * 1991-09-04 1996-04-09 Daikin Industries, Ltd. In situ hybridization method
US5521061A (en) * 1992-07-17 1996-05-28 Aprogenex, Inc. Enhancement of probe signal in nucleic acid-mediated in-situ hybridization studies
WO1994002642A1 (en) * 1992-07-17 1994-02-03 Aprogenex, Inc. Background-reducing compounds for probe-mediated in-situ fluorimetric assays
US5629147A (en) * 1992-07-17 1997-05-13 Aprogenex, Inc. Enriching and identifying fetal cells in maternal blood for in situ hybridization
US5449604A (en) * 1992-10-21 1995-09-12 University Of Washington Chromosome 14 and familial Alzheimers disease genetic markers and assays
US5582985A (en) * 1994-08-05 1996-12-10 The Regents Of The University Of California Detection of mycobacteria
US5750340A (en) * 1995-04-07 1998-05-12 University Of New Mexico In situ hybridization solution and process
US5888733A (en) * 1995-11-16 1999-03-30 Dako A/S In situ hybridization to detect specific nucleic acid sequences in eucaryotic samples

Also Published As

Publication number Publication date
CA2332510A1 (en) 1999-11-25
CY1110164T1 (el) 2015-01-14
EP1080228A1 (de) 2001-03-07
US6165723A (en) 2000-12-26
ES2344873T3 (es) 2010-09-08
WO1999060163A1 (en) 1999-11-25
CA2332510C (en) 2011-07-26
EP1080228A4 (de) 2005-01-12
AU4087399A (en) 1999-12-06
ATE466099T1 (de) 2010-05-15
EP1080228B1 (de) 2010-04-28
DK1080228T3 (da) 2010-08-16
PT1080228E (pt) 2010-07-22

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