DE69834908D1 - Verfahren zur erhöhung der expression von endogenen oder exogenen genprodukten in pflanzen - Google Patents

Verfahren zur erhöhung der expression von endogenen oder exogenen genprodukten in pflanzen

Info

Publication number
DE69834908D1
DE69834908D1 DE69834908T DE69834908T DE69834908D1 DE 69834908 D1 DE69834908 D1 DE 69834908D1 DE 69834908 T DE69834908 T DE 69834908T DE 69834908 T DE69834908 T DE 69834908T DE 69834908 D1 DE69834908 D1 DE 69834908D1
Authority
DE
Germany
Prior art keywords
expression
plants
genes
plant
endogenic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69834908T
Other languages
English (en)
Other versions
DE69834908T2 (de
Inventor
Vicki B Vance
James Carrington
Gail J Pruss
Laszlo Marton
William O Dawson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of South Carolina
Original Assignee
University of South Carolina
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of South Carolina filed Critical University of South Carolina
Application granted granted Critical
Publication of DE69834908D1 publication Critical patent/DE69834908D1/de
Publication of DE69834908T2 publication Critical patent/DE69834908T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8203Virus mediated transformation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/503Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from viruses
    • C12N9/506Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from viruses derived from RNA viruses
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011Details
    • C12N2770/34011Potyviridae
    • C12N2770/34022New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Virology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • General Preparation And Processing Of Foods (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DE69834908T 1997-03-28 1998-03-27 Verfahren zur erhöhung der expression von endogenen oder exogenen genprodukten in pflanzen Expired - Lifetime DE69834908T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US08/827,575 US5939541A (en) 1997-03-28 1997-03-28 Method for enhancing expression of a foreign or endogenous gene product in plants
PCT/US1998/006075 WO1998044097A2 (en) 1997-03-28 1998-03-27 Method for enhancing expression of a foreign or endogenous gene product in plants
US827575 2001-04-06

Publications (2)

Publication Number Publication Date
DE69834908D1 true DE69834908D1 (de) 2006-07-27
DE69834908T2 DE69834908T2 (de) 2007-02-01

Family

ID=25249575

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69834908T Expired - Lifetime DE69834908T2 (de) 1997-03-28 1998-03-27 Verfahren zur erhöhung der expression von endogenen oder exogenen genprodukten in pflanzen

Country Status (7)

Country Link
US (1) US5939541A (de)
EP (1) EP0970200B1 (de)
AT (1) ATE330007T1 (de)
AU (1) AU6871398A (de)
CA (1) CA2285026C (de)
DE (1) DE69834908T2 (de)
WO (1) WO1998044097A2 (de)

Families Citing this family (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6395962B1 (en) * 1999-06-22 2002-05-28 University Of South Carolina Enhancing expression of a silenced target sequence in plants using plant viral enhancers and amplicons
AU1602301A (en) 1999-11-12 2001-06-06 University Of South Carolina Method for enhancing resistance in plants
US7217854B1 (en) * 1999-11-22 2007-05-15 Plant Bioscience Limited Enhanced transgene expression by co-expression with a suppressor of post-transcriptional gene silencing (PTGS)
EP1349946B1 (de) 2000-08-25 2011-01-26 BASF Plant Science GmbH Polynukleotide, die für prenylproteasen aus pflanzen kodieren
DE10049587A1 (de) 2000-10-06 2002-05-02 Icon Genetics Ag Vektorsystem für Pflanzen
DE10061150A1 (de) * 2000-12-08 2002-06-13 Icon Genetics Ag Verfahren und Vektoren zur Erzeugung von transgenen Pflanzen
DE10102389A1 (de) 2001-01-19 2002-08-01 Icon Genetics Ag Verfahren und Vektoren zur Plastidentransformation höherer Pflanzen
GB0101513D0 (en) * 2001-01-19 2001-03-07 Scottish Crop Res Inst 'Gene silencing suppressor'
DE10114209A1 (de) 2001-03-23 2002-12-05 Icon Genetics Ag Ortsgerichtete Transformation durch Verwendung von Amplifikationsvektoren
ES2391765T3 (es) 2001-03-28 2012-11-29 Consejo Superior Investigaciones Cientificas (Csic) Método para mejorar la tolerancia de las plantas al estrés ambiental
DE10115507A1 (de) 2001-03-29 2002-10-10 Icon Genetics Ag Verfahren zur Kodierung von Information in Nukleinsäuren eines genetisch veränderten Organismus
US7122720B2 (en) 2001-04-24 2006-10-17 Cornell Research Foundation, Inc. Synthetic nucleic acid molecule for imparting multiple traits
DE10121283B4 (de) 2001-04-30 2011-08-11 Icon Genetics GmbH, 80333 Verfahren und Vektoren zur Amplifikation oder Expression von gewünschten Nucleinsäuresequenzen in Pflanzen
DE10132780A1 (de) 2001-07-06 2003-01-16 Icon Genetics Ag Plastidäre Genexpression über autonom replizierende Vektoren
US7001739B2 (en) * 2001-08-24 2006-02-21 The Texas A&M University System Isolation of proteins involved in posttranscriptional gene silencing and methods of use
DE10143238A1 (de) 2001-09-04 2003-03-20 Icon Genetics Ag Identifizierung eukaryotischer interner Ribosomen-Eingangsstellen (IRES)-Elemente
DE10143237A1 (de) 2001-09-04 2003-03-20 Icon Genetics Ag Herstellung künstlicher interner ribosomaler Eingangsstellenelemente (Ires-Elemente)
CA2475388A1 (en) * 2002-02-14 2003-08-21 William J. Rutter Chimeric molecules for cleavage in a treated host
US20040268441A1 (en) * 2002-07-19 2004-12-30 University Of South Carolina Compositions and methods for the modulation of gene expression in plants
WO2004044161A2 (en) * 2002-11-06 2004-05-27 Fraunhofer Usa Expression of foreign sequences in plants using trans-activation system
US7683238B2 (en) * 2002-11-12 2010-03-23 iBio, Inc. and Fraunhofer USA, Inc. Production of pharmaceutically active proteins in sprouted seedlings
US7692063B2 (en) * 2002-11-12 2010-04-06 Ibio, Inc. Production of foreign nucleic acids and polypeptides in sprout systems
WO2004070016A2 (en) * 2003-02-03 2004-08-19 Fraunhofer Usa Inc. System for expression of genes in plants
US7674953B2 (en) 2003-04-01 2010-03-09 Cropdesign N.V. Method for producing transgenic plants with increased yield, comprising expressing of haemoglobin from Arabidopsis
CA2555230A1 (en) * 2004-02-20 2005-09-09 Fraunhofer Usa Inc. Systems and methods for clonal expression in plants
ES2494840T3 (es) 2005-03-15 2014-09-16 Fujirebio Europe N.V. Variantes víricas de la hepatitis B con susceptibilidad reducida a análogos de nucleósidos y usos de las mismas
US20110138495A1 (en) * 2005-07-05 2011-06-09 Arthur Weissinger Methods and Compositions for Expressing Proteins In Plants
CA2640473A1 (en) * 2006-02-03 2007-08-16 The Texas A & M University System Enhancing expression of value-added genes by transgenic expression of tombusvirus-based p19 gene mutants
SI23374A (sl) 2010-05-24 2011-11-30 Nacionalni@inštitut@za@biologijo Uporaba glikozidaz in glikoziltransferaz za povečano proizvodnjo proteinov
FI20100248A0 (fi) * 2010-06-11 2010-06-11 Kristiina Maekinen Menetelmä proteiinien tuottamiseksi
SG192022A1 (en) 2011-01-17 2013-08-30 Philip Morris Prod Protein expression in plants
US20140059718A1 (en) 2011-01-17 2014-02-27 Philip Morris Products S.A. Vectors for nucleic acid expression in plants
EP2801619A1 (de) 2013-05-10 2014-11-12 Fondazione Edmund Mach Verfahren zur Erhöhung der Photosynthese mittels Polypeptid mit Curvature-Thylakoid-1 (CURT1)
WO2019049111A1 (en) 2017-09-11 2019-03-14 R. J. Reynolds Tobacco Company METHODS AND COMPOSITIONS FOR INCREASING THE EXPRESSION OF GENES OF INTEREST IN A PLANT BY CO-EXPRESSION WITH P21

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6054684A (ja) * 1983-09-05 1985-03-29 Teijin Ltd 新規dνa及びハイブリツドdνa
US5316931A (en) * 1988-02-26 1994-05-31 Biosource Genetics Corp. Plant viral vectors having heterologous subgenomic promoters for systemic expression of foreign genes
US5491076A (en) * 1993-11-01 1996-02-13 The Texas A&M University System Expression of foreign genes using a replicating polyprotein producing virus vector
US6020169A (en) * 1995-07-20 2000-02-01 Washington State University Research Foundation Production of secreted foreign polypeptides in plant cell culture

Also Published As

Publication number Publication date
DE69834908T2 (de) 2007-02-01
CA2285026A1 (en) 1998-10-08
US5939541A (en) 1999-08-17
WO1998044097A3 (en) 1998-12-30
AU6871398A (en) 1998-10-22
EP0970200A2 (de) 2000-01-12
CA2285026C (en) 2011-03-01
WO1998044097A2 (en) 1998-10-08
EP0970200B1 (de) 2006-06-14
ATE330007T1 (de) 2006-07-15

Similar Documents

Publication Publication Date Title
DE69834908D1 (de) Verfahren zur erhöhung der expression von endogenen oder exogenen genprodukten in pflanzen
DE69534421D1 (de) Cytoplasmatische Inhibition des Genexpression
ATE465266T1 (de) Zusammensetzungen und verfahren zur verpackung von alpavirus-vektoren
DE60236182D1 (de) Zufällige integration von polynukleotide nach in vivo linearisierung
DE69230993D1 (de) Rekombinante viren vektoren zur expression in muskelzellen
ES2170622A1 (es) Clones y vectores infectivos derivados de coronavirus y sus aplicaciones.
DE69333529D1 (de) Pflanzenvirus-vektor, plasmid, methode der expression fremder gene und methode zur gewinnung obiger genprodukte
ATE325533T1 (de) Verfahren zum verbesserten virusvermittelten dns- transfer unter verwendung von molekülen mit virus-und zellbindenden domänen
DE69638103D1 (de) Die transduktion von myoblasten mittels vektoren aus adenoassoziierten viren
BR9007566A (pt) Metodo de preparacao de plasmida,plasmida e produtos de expressao de um plasmida
DE60128149D1 (en) Putrescin-n-methyltransferasepromotor
PT835137E (pt) Celulas encapsuladas produtoras de particulas virais
NZ307444A (en) Polyphenol oxidase genes from lettuce and banana
ATE284446T1 (de) Herstellung von trehalose in pflanzen
ATE173762T1 (de) Klonierung der n-methylhydantoinase
CO4480058A1 (es) Genes codificantes y procedimiento de expresion de una nueva enzima: ftalil amidasa
ATE162852T1 (de) Biosynthetisches verfahren zur herstellung von protein
ATE353368T1 (de) Verfahren zur herstellung verzweigtkettiger fettsäuren mittels genetisch modifizierter pflanzen
DE59807664D1 (de) Verfahren zur herstellung von 3-cyano-2,4-dihalogen-5-fluor-benzoesäuren
HUP0202805A2 (hu) Célzott géneltávolítás
GB2366291A (en) A transformant for screening of inhibitors for human immnodeficiency virus
DE50109097D1 (de) Promotoren zur genexpression in karyopsen von pflanzen
DE60032162D1 (de) Regulatorische sequenzen von funktioneller bedeutung in filamentösen pilzen
DK0966533T3 (da) Aktivitetsafhængig neurotrof faktor III (ADNF III).
DE59902472D1 (de) Zusammensetzung zur induktion einer tumorspezifischen immunantwort, verfahren zu deren herstellung sowie verwendung der zusammensetzung zur behandlung von neoplasien

Legal Events

Date Code Title Description
8364 No opposition during term of opposition