DE69813243T2 - Synthese von oligonukleotiden in lösung - Google Patents

Synthese von oligonukleotiden in lösung Download PDF

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Publication number
DE69813243T2
DE69813243T2 DE69813243T DE69813243T DE69813243T2 DE 69813243 T2 DE69813243 T2 DE 69813243T2 DE 69813243 T DE69813243 T DE 69813243T DE 69813243 T DE69813243 T DE 69813243T DE 69813243 T2 DE69813243 T2 DE 69813243T2
Authority
DE
Germany
Prior art keywords
phosphonate
synthesis
solution
oligonucleotids
alcohol
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69813243T
Other languages
German (de)
English (en)
Other versions
DE69813243D1 (de
Inventor
Colin Bernard Reese
Quanlai Song
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nitto Denko Avecia Inc
Original Assignee
Avecia Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Avecia Ltd filed Critical Avecia Ltd
Publication of DE69813243D1 publication Critical patent/DE69813243D1/de
Application granted granted Critical
Publication of DE69813243T2 publication Critical patent/DE69813243T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D265/00—Heterocyclic compounds containing six-membered rings having one nitrogen atom and one oxygen atom as the only ring hetero atoms
    • C07D265/28—1,4-Oxazines; Hydrogenated 1,4-oxazines
    • C07D265/30—1,4-Oxazines; Hydrogenated 1,4-oxazines not condensed with other rings
    • C07D265/32—1,4-Oxazines; Hydrogenated 1,4-oxazines not condensed with other rings with oxygen atoms directly attached to ring carbon atoms
    • C07D265/33—Two oxygen atoms, in positions 3 and 5
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D207/00—Heterocyclic compounds containing five-membered rings not condensed with other rings, with one nitrogen atom as the only ring hetero atom
    • C07D207/46—Heterocyclic compounds containing five-membered rings not condensed with other rings, with one nitrogen atom as the only ring hetero atom with hetero atoms directly attached to the ring nitrogen atom
    • C07D207/48—Sulfur atoms
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic Table
    • C07F9/02—Phosphorus compounds
    • C07F9/06—Phosphorus compounds without P—C bonds
    • C07F9/16—Esters of thiophosphoric acids or thiophosphorous acids
    • C07F9/165—Esters of thiophosphoric acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic Table
    • C07F9/02—Phosphorus compounds
    • C07F9/06—Phosphorus compounds without P—C bonds
    • C07F9/16—Esters of thiophosphoric acids or thiophosphorous acids
    • C07F9/165—Esters of thiophosphoric acids
    • C07F9/18—Esters of thiophosphoric acids with hydroxyaryl compounds
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H11/00—Compounds containing saccharide radicals esterified by inorganic acids; Metal salts thereof
    • C07H11/04—Phosphates; Phosphites; Polyphosphates
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06—Pyrimidine radicals
    • C07H19/10—Pyrimidine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/16—Purine radicals
    • C07H19/20—Purine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/55—Design of synthesis routes, e.g. reducing the use of auxiliary or protecting groups

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Saccharide Compounds (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DE69813243T 1997-08-13 1998-08-10 Synthese von oligonukleotiden in lösung Expired - Fee Related DE69813243T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB9717158.1A GB9717158D0 (en) 1997-08-13 1997-08-13 Solution synthesis of oligonucleotides and their phosphorothioate analogues
PCT/GB1998/002407 WO1999009041A2 (en) 1997-08-13 1998-08-10 Solution phase synthesis of oligonucleotides

Publications (2)

Publication Number Publication Date
DE69813243D1 DE69813243D1 (de) 2003-05-15
DE69813243T2 true DE69813243T2 (de) 2004-01-29

Family

ID=10817430

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69813243T Expired - Fee Related DE69813243T2 (de) 1997-08-13 1998-08-10 Synthese von oligonukleotiden in lösung

Country Status (21)

Country Link
US (3) US6506894B1 (enExample)
EP (2) EP1325927A3 (enExample)
JP (1) JP2001515086A (enExample)
KR (1) KR20010022840A (enExample)
CN (1) CN1231492C (enExample)
AT (1) ATE236916T1 (enExample)
BR (1) BR9811166A (enExample)
CA (1) CA2300874A1 (enExample)
DE (1) DE69813243T2 (enExample)
DK (1) DK1003758T3 (enExample)
EA (1) EA003091B1 (enExample)
ES (1) ES2197491T3 (enExample)
GB (1) GB9717158D0 (enExample)
HU (1) HUP0002684A3 (enExample)
IL (1) IL134517A (enExample)
IN (1) IN190784B (enExample)
NO (1) NO20000690L (enExample)
NZ (1) NZ502684A (enExample)
PL (1) PL338888A1 (enExample)
TR (1) TR200000384T2 (enExample)
WO (1) WO1999009041A2 (enExample)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9924285D0 (en) * 1999-10-14 1999-12-15 Avecia Ltd Process
GB0004889D0 (en) * 2000-03-01 2000-04-19 Avecia Ltd Synthesis of oligonucleotides
AU2002339175A1 (en) * 2001-11-21 2003-06-10 Avecia Biotechnology Inc. Coupling reagent for h-phosphonate chemistry
JP2005518451A (ja) * 2002-02-22 2005-06-23 ハネウェル・インターナショナル・インコーポレーテッド ホスフィチル化化合物を製造する方法
GB0209539D0 (en) * 2002-04-26 2002-06-05 Avecia Ltd Monomer Polymer and process
WO2005077966A1 (en) * 2004-02-10 2005-08-25 Isis Pharmaceuticals, Inc. Substituted pixyl protecting groups for oligonucleotide synthesis
SG171914A1 (en) 2008-12-02 2011-07-28 Chiralgen Ltd Method for the synthesis of phosphorus atom modified nucleic acids
US8272150B2 (en) * 2008-12-12 2012-09-25 Speedplay, Inc. Shoe sole mounting standard for bicycle cleat
JP2012513450A (ja) 2008-12-23 2012-06-14 ギリンダス・アメリカ・インコーポレイテッド 硫化剤およびオリゴヌクレオチドを合成するためのその使用
SG10201403841QA (en) 2009-07-06 2014-09-26 Ontorii Inc Novel nucleic acid prodrugs and methods of use thereof
CN103298823A (zh) 2010-06-30 2013-09-11 集润德斯股份公司 一种使用n-硫代化合物用于寡核苷酸合成的新方法
DK2620428T3 (da) 2010-09-24 2019-07-01 Wave Life Sciences Ltd Asymmetrisk hjælpegruppe
EP2734208B1 (en) 2011-07-19 2017-03-01 Wave Life Sciences Ltd. Methods for the synthesis of functionalized nucleic acids
WO2013017469A1 (en) 2011-07-29 2013-02-07 Girindus America, Inc. Sulfurization reagents on solid supports
SG11201500243WA (en) 2012-07-13 2015-04-29 Shin Nippon Biomedical Lab Ltd Chiral nucleic acid adjuvant
JP6453212B2 (ja) 2012-07-13 2019-01-16 ウェイブ ライフ サイエンシズ リミテッドＷａｖｅ Ｌｉｆｅ Ｓｃｉｅｎｃｅｓ Ｌｔｄ． キラル制御
CN104684893B (zh) 2012-07-13 2016-10-26 日本波涛生命科学公司 不对称辅助基团
WO2014085471A1 (en) * 2012-11-28 2014-06-05 Prognosdx Health, Inc. Acid-activated compositions for the treatment of cancers, methods of their use and methods of their preparation
US10144933B2 (en) 2014-01-15 2018-12-04 Shin Nippon Biomedical Laboratories, Ltd. Chiral nucleic acid adjuvant having immunity induction activity, and immunity induction activator
US10322173B2 (en) 2014-01-15 2019-06-18 Shin Nippon Biomedical Laboratories, Ltd. Chiral nucleic acid adjuvant having anti-allergic activity, and anti-allergic agent
US10149905B2 (en) 2014-01-15 2018-12-11 Shin Nippon Biomedical Laboratories, Ltd. Chiral nucleic acid adjuvant having antitumor effect and antitumor agent
MX2016009290A (es) 2014-01-16 2017-02-28 Wave Life Sciences Ltd Diseño quiral.
WO2018215049A1 (en) * 2017-05-23 2018-11-29 F. Hoffmann-La Roche Ag Process for galnac oligonucleotide conjugates

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3444300A (en) * 1964-05-05 1969-05-13 Chevron Res N-polyhaloalkylthio compounds as fungicidal agents
FR1519704A (fr) * 1967-02-14 1968-04-05 Roussel Uclaf Nouveaux composés dithiocarbamiques hétérocycliques et procédé de préparation
US3546185A (en) 1968-03-20 1970-12-08 Monsanto Co Inhibiting premature vulcanization of diene rubbers
US5149798A (en) * 1989-04-06 1992-09-22 Worcester Foundation For Experimental Biology Process for synthesizing oligonucleotides and their analogs adaptable to large scale syntheses
CN1122137A (zh) * 1993-01-08 1996-05-08 海布里顿公司 二聚体块的合成及其在装配寡核苷酸中的应用
GB9501465D0 (en) 1995-01-25 1995-03-15 King S College London Nucleoside phosphorothioate derivatives,synthesis and use thereof
GB0004889D0 (en) * 2000-03-01 2000-04-19 Avecia Ltd Synthesis of oligonucleotides

Also Published As

Publication number Publication date
EA200000211A1 (ru) 2000-08-28
EP1325927A2 (en) 2003-07-09
ATE236916T1 (de) 2003-04-15
GB9717158D0 (en) 1997-10-22
DK1003758T3 (da) 2003-07-21
DE69813243D1 (de) 2003-05-15
EP1003758A2 (en) 2000-05-31
NZ502684A (en) 2002-12-20
IN190784B (enExample) 2003-08-23
HUP0002684A2 (hu) 2000-12-28
BR9811166A (pt) 2000-07-25
EP1003758B1 (en) 2003-04-09
ES2197491T3 (es) 2004-01-01
CN1274361A (zh) 2000-11-22
IL134517A0 (en) 2001-04-30
WO1999009041A2 (en) 1999-02-25
EP1325927A3 (en) 2008-07-02
US20030083490A1 (en) 2003-05-01
JP2001515086A (ja) 2001-09-18
PL338888A1 (en) 2000-11-20
US7019127B2 (en) 2006-03-28
HUP0002684A3 (en) 2001-11-28
TR200000384T2 (tr) 2000-11-21
NO20000690L (no) 2000-04-11
AU8738698A (en) 1999-03-08
AU749343B2 (en) 2002-06-27
EA003091B1 (ru) 2002-12-26
KR20010022840A (ko) 2001-03-26
CN1231492C (zh) 2005-12-14
US6506894B1 (en) 2003-01-14
WO1999009041A3 (en) 1999-05-06
NO20000690D0 (no) 2000-02-11
US20050240015A1 (en) 2005-10-27
IL134517A (en) 2004-02-19
CA2300874A1 (en) 1999-02-25

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8327 Change in the person/name/address of the patent owner

Owner name: AVECIA BIOTECHNOLOGY, INC., MILFORD, MASS., US

8328 Change in the person/name/address of the agent

Representative=s name: HOFFMANN & EITLE, 81925 MUENCHEN

8339 Ceased/non-payment of the annual fee