DE69738354D1 - Verwendung bestimmter Substanzen für das Verbessern der Wärmestabilität von RNS - Google Patents

Verwendung bestimmter Substanzen für das Verbessern der Wärmestabilität von RNS

Info

Publication number
DE69738354D1
DE69738354D1 DE69738354T DE69738354T DE69738354D1 DE 69738354 D1 DE69738354 D1 DE 69738354D1 DE 69738354 T DE69738354 T DE 69738354T DE 69738354 T DE69738354 T DE 69738354T DE 69738354 D1 DE69738354 D1 DE 69738354D1
Authority
DE
Germany
Prior art keywords
rna
improving
thermal stability
certain substances
substances
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69738354T
Other languages
English (en)
Inventor
Yoshihide Hayashizaki
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Dnaform KK
RIKEN Institute of Physical and Chemical Research
Original Assignee
Dnaform KK
RIKEN Institute of Physical and Chemical Research
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from JP19633196A external-priority patent/JP3709490B2/ja
Application filed by Dnaform KK, RIKEN Institute of Physical and Chemical Research filed Critical Dnaform KK
Application granted granted Critical
Publication of DE69738354D1 publication Critical patent/DE69738354D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DE69738354T 1996-07-25 1997-07-24 Verwendung bestimmter Substanzen für das Verbessern der Wärmestabilität von RNS Expired - Lifetime DE69738354D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP19632996 1996-07-25
JP19633196A JP3709490B2 (ja) 1996-07-25 1996-07-25 Ｒｎａの熱安定性の改良方法

Publications (1)

Publication Number Publication Date
DE69738354D1 true DE69738354D1 (de) 2008-01-17

Family

ID=26509679

Family Applications (2)

Application Number Title Priority Date Filing Date
DE69738354T Expired - Lifetime DE69738354D1 (de) 1996-07-25 1997-07-24 Verwendung bestimmter Substanzen für das Verbessern der Wärmestabilität von RNS
DE69734913T Expired - Lifetime DE69734913T2 (de) 1996-07-25 1997-07-24 Verfahren für reverse Transkription

Family Applications After (1)

Application Number Title Priority Date Filing Date
DE69734913T Expired - Lifetime DE69734913T2 (de) 1996-07-25 1997-07-24 Verfahren für reverse Transkription

Country Status (4)

Country Link
US (3) US6013488A (de)
EP (2) EP0821059B8 (de)
CA (1) CA2211367C (de)
DE (2) DE69738354D1 (de)

Families Citing this family (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2287542A1 (en) * 1997-04-22 1998-10-29 Life Technologies, Inc. Methods for the production of aslv reverse transcriptases composed of multiple subunits
US6268133B1 (en) 1997-06-25 2001-07-31 Invitrogen Corporation Method for isolating and recovering target DNA or RNA molecules having a desired nucleotide sequence
US6787305B1 (en) 1998-03-13 2004-09-07 Invitrogen Corporation Compositions and methods for enhanced synthesis of nucleic acid molecules
PT1129064E (pt) 1998-11-12 2008-01-31 Invitrogen Corp Reagentes de transfecção
US7074556B2 (en) * 1999-03-02 2006-07-11 Invitrogen Corporation cDNA synthesis improvements
EP1165760A2 (de) * 1999-03-12 2002-01-02 McGill University Verfahren zur erhöhung der prozessivität der dns- oder rns-abhängigen polymerase und zusammensetzungen dafür
US6271004B1 (en) * 1999-06-25 2001-08-07 Display Systems Biotech A/S Method for improved reverse transcription at high temperatures
US6835537B1 (en) 2000-09-29 2004-12-28 Myriad Genetics, Inc. Method for equalizing band intensities on sequencing gels
DE10105208A1 (de) * 2001-02-06 2002-08-14 Ihf Inst Fuer Hormon Und Fortp Verfahren zur Herstellung von cDNA
ES2188357B1 (es) * 2001-03-12 2005-01-01 BIOTOOLS BIOTECHNOLOGICAL & MEDICAL LABORATORIES, S.A. Metodo para la amplificacion mediante transcripcion en reverso acoplada a una reaccion de nested o semi-nested pcr en un solo paso, de un arn diana, y sus aplicaciones en la identificacion de enterovirus y en la discriminacion entre poliovirus y enterovirus no-polio.
AU2003238702A1 (en) * 2002-06-12 2003-12-31 Kabushiki Kaisha Dnaform Method of utilizing the 5'end of transcribed nucleic acid regions for cloning and analysis
US20040152072A1 (en) * 2002-07-30 2004-08-05 Invitrogen Corporation Reverse transcription
US20060084799A1 (en) * 2003-09-24 2006-04-20 Williams Lewis T Human cDNA clones comprising polynucleotides encoding polypeptides and methods of their use
WO2005035569A2 (en) * 2003-10-10 2005-04-21 Five Prime Therapeutics, Inc. Kiaa0779, splice variants thereof, and methods of their use
CA2560513A1 (en) 2004-04-08 2005-12-01 Biomatrica, Inc. Integration of sample storage and sample management for life science
US20060099567A1 (en) * 2004-04-08 2006-05-11 Biomatrica, Inc. Integration of sample storage and sample management for life science
US20080176209A1 (en) * 2004-04-08 2008-07-24 Biomatrica, Inc. Integration of sample storage and sample management for life science
EP1877581B1 (de) 2005-05-06 2012-03-28 Gen-Probe Incorporated Verfahren und produkte zum erfassen von nukleinsäurezielen
WO2007011901A2 (en) * 2005-07-15 2007-01-25 Applera Corporation Hot start reverse transcription by primer design
WO2012018639A2 (en) 2010-07-26 2012-02-09 Biomatrica, Inc. Compositions for stabilizing dna, rna and proteins in saliva and other biological samples during shipping and storage at ambient temperatures
EP2598660B1 (de) 2010-07-26 2017-03-15 Biomatrica, INC. Zusammensetzungen zur stabilisierung von dna, rna und proteinen in blut- und anderen biologischen proben während des transports und der lagerung bei umgebungstemperaturen
US9458484B2 (en) 2010-10-22 2016-10-04 Bio-Rad Laboratories, Inc. Reverse transcriptase mixtures with improved storage stability
FR2984357B1 (fr) * 2011-12-16 2016-11-18 Biomerieux Sa Procede d'amplification transcriptionnelle d'acides nucleiques reunissant des etapes de temperatures differentes
US9725703B2 (en) 2012-12-20 2017-08-08 Biomatrica, Inc. Formulations and methods for stabilizing PCR reagents
KR20160113177A (ko) 2014-01-22 2016-09-28 라이프 테크놀로지스 코포레이션 고온 핵산 합성에서 이용하기 위한 신규한 역전사효소
EP3154338B1 (de) 2014-06-10 2020-01-29 Biomatrica, INC. Stabilisierung von thrombozyten bei umgebungstemperatur
WO2016011203A1 (en) 2014-07-15 2016-01-21 Life Technologies Corporation Compositions with lipid aggregates and methods for efficient delivery of molecules to cells
JP6827048B2 (ja) 2015-12-08 2021-02-10 バイオマトリカ，インク． 赤血球沈降速度の低下
JP6417603B2 (ja) 2016-01-27 2018-11-07 株式会社ダナフォーム Ｒｎａの末端領域に対応する核酸の塩基配列を解読する方法およびｄｎａエレメントの分析方法
WO2021020245A1 (ja) * 2019-07-26 2021-02-04 東洋紡株式会社 安定性に優れた変異型逆転写酵素

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0035204B2 (de) * 1980-03-05 1991-05-22 Miles Inc. Pasteurisierte therapeutisch aktive Proteinzusammensetzungen
US4409200A (en) * 1980-04-28 1983-10-11 Research Corporation Reverse transcriptase from human milk, method for its purification, and its use in the detection of breast cancer
US5017492A (en) * 1986-02-27 1991-05-21 Life Technologies, Inc. Reverse transcriptase and method for its production
US5310652A (en) * 1986-08-22 1994-05-10 Hoffman-La Roche Inc. Reverse transcription with thermostable DNA polymerase-high temperature reverse transcription
US5374553A (en) * 1986-08-22 1994-12-20 Hoffmann-La Roche Inc. DNA encoding a thermostable nucleic acid polymerase enzyme from thermotoga maritima
US5407800A (en) * 1986-08-22 1995-04-18 Hoffmann-La Roche Inc. Reverse transcription with Thermus thermophilus polymerase
DK0506889T3 (da) * 1989-12-22 1997-09-22 Hoffmann La Roche Reverse transkriptaser ved høj temperatur
DE4124286A1 (de) * 1991-07-22 1993-01-28 Boehringer Mannheim Gmbh Verfahren zur stabilisierung von proteinen bei optischen tests
FR2690691B1 (fr) * 1992-04-29 1999-02-12 Bio Merieux Procede d'amplification d'arn necessitant une seule etape de manipulation.
WO1994003472A1 (en) * 1992-08-04 1994-02-17 Gen-Probe Incorporated Nucleic acid sequence amplification
WO1994012657A1 (en) * 1992-11-20 1994-06-09 Katcher Harold L Direct-phenol pcr, rt and rt-pcr methods
DE4239969A1 (de) * 1992-11-27 1994-06-01 Boehringer Mannheim Gmbh Verfahren zur Stabilisierung von Proteinen
CA2139623A1 (en) * 1993-05-06 1994-11-24 Baxter Diagnostics Inc. Human papillomavirus detection assay
EP0730642A1 (de) * 1993-11-25 1996-09-11 Pacific Enzymes Limited Verbesserte polymerase
EP0742838B1 (de) * 1994-01-31 2003-08-13 The Regents Of The University Of California Methode zur eliminierung von sequenzierartefakten
DE4411588C1 (de) * 1994-03-30 1995-09-28 Deutsches Rheuma Forschungszen Puffer für DNA- und RNA-Polymerase-Reaktionen
US5556771A (en) * 1995-02-10 1996-09-17 Gen-Probe Incorporated Stabilized compositions of reverse transcriptase and RNA polymerase for nucleic acid amplification

Also Published As

Publication number Publication date
US6013488A (en) 2000-01-11
US6221599B1 (en) 2001-04-24
EP1243661B1 (de) 2007-12-05
EP0821059B8 (de) 2006-03-08
DE69734913T2 (de) 2006-08-24
DE69734913D1 (de) 2006-01-26
EP0821059B1 (de) 2005-12-21
EP0821059A2 (de) 1998-01-28
CA2211367C (en) 2008-04-22
US6372437B2 (en) 2002-04-16
US20010012617A1 (en) 2001-08-09
EP0821059A3 (de) 1998-04-22
CA2211367A1 (en) 1998-01-25
EP1243661A1 (de) 2002-09-25

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Legal Events

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8332 No legal effect for de