DE69732255T2 - Verfahren und vorrichtungen zum nachweis von bakteriophagen - Google Patents
Verfahren und vorrichtungen zum nachweis von bakteriophagen Download PDFInfo
- Publication number
- DE69732255T2 DE69732255T2 DE69732255T DE69732255T DE69732255T2 DE 69732255 T2 DE69732255 T2 DE 69732255T2 DE 69732255 T DE69732255 T DE 69732255T DE 69732255 T DE69732255 T DE 69732255T DE 69732255 T2 DE69732255 T2 DE 69732255T2
- Authority
- DE
- Germany
- Prior art keywords
- dye
- bacteria
- bacteriophages
- precipitable
- bacteriophage
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 241001515965 unidentified phage Species 0.000 title claims description 133
- 238000000034 method Methods 0.000 title claims description 47
- 230000001580 bacterial effect Effects 0.000 claims description 84
- 241000894006 Bacteria Species 0.000 claims description 81
- 238000012360 testing method Methods 0.000 claims description 57
- 239000007788 liquid Substances 0.000 claims description 51
- 239000000463 material Substances 0.000 claims description 28
- 239000002244 precipitate Substances 0.000 claims description 27
- 102000004190 Enzymes Human genes 0.000 claims description 26
- 108090000790 Enzymes Proteins 0.000 claims description 26
- 235000015097 nutrients Nutrition 0.000 claims description 24
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 24
- 238000001514 detection method Methods 0.000 claims description 17
- 150000003839 salts Chemical class 0.000 claims description 13
- 241000588724 Escherichia coli Species 0.000 claims description 12
- 239000003795 chemical substances by application Substances 0.000 claims description 10
- 230000015572 biosynthetic process Effects 0.000 claims description 7
- 238000003776 cleavage reaction Methods 0.000 claims description 7
- 230000002255 enzymatic effect Effects 0.000 claims description 7
- 230000007017 scission Effects 0.000 claims description 7
- 241000607142 Salmonella Species 0.000 claims description 6
- 241000295644 Staphylococcaceae Species 0.000 claims description 6
- 230000010076 replication Effects 0.000 claims description 6
- SCBBSJMAPKXHAH-UOYQFSTFSA-N CO[C@@H]1[C@@H](O)[C@@H](O)O[C@H](CO)[C@@H]1O Chemical compound CO[C@@H]1[C@@H](O)[C@@H](O)O[C@H](CO)[C@@H]1O SCBBSJMAPKXHAH-UOYQFSTFSA-N 0.000 claims description 5
- 241000588914 Enterobacter Species 0.000 claims description 5
- 241000186781 Listeria Species 0.000 claims description 5
- 241000186359 Mycobacterium Species 0.000 claims description 5
- 239000002253 acid Substances 0.000 claims description 5
- BPHPUYQFMNQIOC-NXRLNHOXSA-N isopropyl beta-D-thiogalactopyranoside Chemical compound CC(C)S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O BPHPUYQFMNQIOC-NXRLNHOXSA-N 0.000 claims description 5
- HOVAGTYPODGVJG-XUUWZHRGSA-N methyl beta-D-glucopyranoside Chemical compound CO[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O HOVAGTYPODGVJG-XUUWZHRGSA-N 0.000 claims description 5
- 230000001939 inductive effect Effects 0.000 claims description 4
- 239000000047 product Substances 0.000 claims description 3
- 230000006872 improvement Effects 0.000 claims description 2
- 230000014670 detection of bacterium Effects 0.000 claims 1
- 238000010186 staining Methods 0.000 claims 1
- 239000000975 dye Substances 0.000 description 133
- 239000000523 sample Substances 0.000 description 70
- 239000000843 powder Substances 0.000 description 50
- 229920001817 Agar Polymers 0.000 description 43
- 239000008272 agar Substances 0.000 description 43
- 239000003349 gelling agent Substances 0.000 description 32
- 239000010408 film Substances 0.000 description 20
- 239000000853 adhesive Substances 0.000 description 18
- 230000001070 adhesive effect Effects 0.000 description 17
- 239000002609 medium Substances 0.000 description 17
- 125000006850 spacer group Chemical group 0.000 description 14
- 239000000203 mixture Substances 0.000 description 13
- 238000012800 visualization Methods 0.000 description 13
- 239000000758 substrate Substances 0.000 description 12
- 239000007787 solid Substances 0.000 description 11
- 239000000243 solution Substances 0.000 description 11
- 241000700605 Viruses Species 0.000 description 10
- 239000011248 coating agent Substances 0.000 description 10
- 238000000576 coating method Methods 0.000 description 10
- 239000012895 dilution Substances 0.000 description 10
- 238000010790 dilution Methods 0.000 description 10
- 230000003612 virological effect Effects 0.000 description 9
- 108010031186 Glycoside Hydrolases Proteins 0.000 description 8
- 102000005744 Glycoside Hydrolases Human genes 0.000 description 8
- 238000003556 assay Methods 0.000 description 8
- 239000000411 inducer Substances 0.000 description 8
- 108090000371 Esterases Proteins 0.000 description 7
- 108090000608 Phosphoric Monoester Hydrolases Proteins 0.000 description 7
- 102000004160 Phosphoric Monoester Hydrolases Human genes 0.000 description 7
- 238000004519 manufacturing process Methods 0.000 description 7
- 244000005700 microbiome Species 0.000 description 7
- 239000002245 particle Substances 0.000 description 7
- -1 polypropylene Polymers 0.000 description 7
- 238000011002 quantification Methods 0.000 description 7
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- 239000003431 cross linking reagent Substances 0.000 description 6
- 239000013078 crystal Substances 0.000 description 6
- 239000000499 gel Substances 0.000 description 6
- 230000036571 hydration Effects 0.000 description 5
- 238000006703 hydration reaction Methods 0.000 description 5
- 239000012528 membrane Substances 0.000 description 5
- PGSADBUBUOPOJS-UHFFFAOYSA-N neutral red Chemical compound Cl.C1=C(C)C(N)=CC2=NC3=CC(N(C)C)=CC=C3N=C21 PGSADBUBUOPOJS-UHFFFAOYSA-N 0.000 description 5
- FLUSEOZMBNGLSB-HNTFPEDGSA-N (2S,3R,4R,5R,6R)-2-bromo-3-chloro-3,4,5,6-tetrahydroxy-4-(1H-indol-2-yl)oxane-2-carboxylic acid Chemical compound O[C@H]1[C@H](O)O[C@](Br)(C(O)=O)[C@](O)(Cl)[C@@]1(O)C1=CC2=CC=CC=C2N1 FLUSEOZMBNGLSB-HNTFPEDGSA-N 0.000 description 4
- OPIFSICVWOWJMJ-AEOCFKNESA-N 5-bromo-4-chloro-3-indolyl beta-D-galactoside Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1OC1=CNC2=CC=C(Br)C(Cl)=C12 OPIFSICVWOWJMJ-AEOCFKNESA-N 0.000 description 4
- QRXMUCSWCMTJGU-UHFFFAOYSA-N 5-bromo-4-chloro-3-indolyl phosphate Chemical compound C1=C(Br)C(Cl)=C2C(OP(O)(=O)O)=CNC2=C1 QRXMUCSWCMTJGU-UHFFFAOYSA-N 0.000 description 4
- 239000004698 Polyethylene Substances 0.000 description 4
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 4
- 239000004820 Pressure-sensitive adhesive Substances 0.000 description 4
- 102000005262 Sulfatase Human genes 0.000 description 4
- 239000003593 chromogenic compound Substances 0.000 description 4
- 150000001875 compounds Chemical class 0.000 description 4
- 238000011109 contamination Methods 0.000 description 4
- 230000009089 cytolysis Effects 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 238000011534 incubation Methods 0.000 description 4
- 230000006698 induction Effects 0.000 description 4
- 239000010410 layer Substances 0.000 description 4
- 239000000178 monomer Substances 0.000 description 4
- 230000035515 penetration Effects 0.000 description 4
- 229920000728 polyester Polymers 0.000 description 4
- 229920000573 polyethylene Polymers 0.000 description 4
- 239000002689 soil Substances 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 108060007951 sulfatase Proteins 0.000 description 4
- 244000303965 Cyamopsis psoralioides Species 0.000 description 3
- 229920002907 Guar gum Polymers 0.000 description 3
- 239000004793 Polystyrene Substances 0.000 description 3
- 239000012790 adhesive layer Substances 0.000 description 3
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 3
- 102000005936 beta-Galactosidase Human genes 0.000 description 3
- 108010005774 beta-Galactosidase Proteins 0.000 description 3
- 229940041514 candida albicans extract Drugs 0.000 description 3
- 238000004040 coloring Methods 0.000 description 3
- 229920001577 copolymer Polymers 0.000 description 3
- 239000012530 fluid Substances 0.000 description 3
- 239000006260 foam Substances 0.000 description 3
- 238000009472 formulation Methods 0.000 description 3
- 239000001963 growth medium Substances 0.000 description 3
- 239000000665 guar gum Substances 0.000 description 3
- 235000010417 guar gum Nutrition 0.000 description 3
- 229960002154 guar gum Drugs 0.000 description 3
- 230000000813 microbial effect Effects 0.000 description 3
- 239000001301 oxygen Substances 0.000 description 3
- 229910052760 oxygen Inorganic materials 0.000 description 3
- 230000035699 permeability Effects 0.000 description 3
- 229920006267 polyester film Polymers 0.000 description 3
- 229920002223 polystyrene Polymers 0.000 description 3
- 238000001556 precipitation Methods 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 239000002356 single layer Substances 0.000 description 3
- 239000011734 sodium Substances 0.000 description 3
- 239000000725 suspension Substances 0.000 description 3
- 239000012138 yeast extract Substances 0.000 description 3
- OPIFSICVWOWJMJ-BCESSLCHSA-N (2s,4r,5s)-2-[(5-bromo-4-chloro-1h-indol-3-yl)oxy]-6-(hydroxymethyl)oxane-3,4,5-triol Chemical compound OC1[C@H](O)[C@H](O)C(CO)O[C@H]1OC1=CNC2=CC=C(Br)C(Cl)=C12 OPIFSICVWOWJMJ-BCESSLCHSA-N 0.000 description 2
- YPFDHNVEDLHUCE-UHFFFAOYSA-N 1,3-propanediol Substances OCCCO YPFDHNVEDLHUCE-UHFFFAOYSA-N 0.000 description 2
- DXPPIEDUBFUSEZ-UHFFFAOYSA-N 6-methylheptyl prop-2-enoate Chemical compound CC(C)CCCCCOC(=O)C=C DXPPIEDUBFUSEZ-UHFFFAOYSA-N 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- QXNVGIXVLWOKEQ-UHFFFAOYSA-N Disodium Chemical class [Na][Na] QXNVGIXVLWOKEQ-UHFFFAOYSA-N 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 102000053187 Glucuronidase Human genes 0.000 description 2
- 108010060309 Glucuronidase Proteins 0.000 description 2
- 241000725303 Human immunodeficiency virus Species 0.000 description 2
- 241000702318 Microviridae Species 0.000 description 2
- 108700019535 Phosphoprotein Phosphatases Proteins 0.000 description 2
- 239000004743 Polypropylene Substances 0.000 description 2
- 241000702202 Siphoviridae Species 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 125000005250 alkyl acrylate group Chemical group 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- 230000010460 detection of virus Effects 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 239000008101 lactose Substances 0.000 description 2
- 239000004816 latex Substances 0.000 description 2
- 229920000126 latex Polymers 0.000 description 2
- 230000002101 lytic effect Effects 0.000 description 2
- 230000008018 melting Effects 0.000 description 2
- 238000002844 melting Methods 0.000 description 2
- 229920000609 methyl cellulose Polymers 0.000 description 2
- DWCZIOOZPIDHAB-UHFFFAOYSA-L methyl green Chemical compound [Cl-].[Cl-].C1=CC(N(C)C)=CC=C1C(C=1C=CC(=CC=1)[N+](C)(C)C)=C1C=CC(=[N+](C)C)C=C1 DWCZIOOZPIDHAB-UHFFFAOYSA-L 0.000 description 2
- 239000001923 methylcellulose Substances 0.000 description 2
- 238000005065 mining Methods 0.000 description 2
- 239000012875 nonionic emulsifier Substances 0.000 description 2
- 239000007764 o/w emulsion Substances 0.000 description 2
- 229920001155 polypropylene Polymers 0.000 description 2
- 229920000166 polytrimethylene carbonate Polymers 0.000 description 2
- 239000001103 potassium chloride Substances 0.000 description 2
- 235000011164 potassium chloride Nutrition 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 230000001737 promoting effect Effects 0.000 description 2
- 230000001681 protective effect Effects 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 239000008213 purified water Substances 0.000 description 2
- OARRHUQTFTUEOS-UHFFFAOYSA-N safranin Chemical compound [Cl-].C=12C=C(N)C(C)=CC2=NC2=CC(C)=C(N)C=C2[N+]=1C1=CC=CC=C1 OARRHUQTFTUEOS-UHFFFAOYSA-N 0.000 description 2
- 239000012898 sample dilution Substances 0.000 description 2
- 229910052708 sodium Inorganic materials 0.000 description 2
- 238000007711 solidification Methods 0.000 description 2
- 230000008023 solidification Effects 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- 235000000346 sugar Nutrition 0.000 description 2
- 239000010409 thin film Substances 0.000 description 2
- 239000012780 transparent material Substances 0.000 description 2
- 241001624918 unidentified bacterium Species 0.000 description 2
- 230000008478 viral entry into host cell Effects 0.000 description 2
- 239000004552 water soluble powder Substances 0.000 description 2
- 239000000230 xanthan gum Substances 0.000 description 2
- 235000010493 xanthan gum Nutrition 0.000 description 2
- 229920001285 xanthan gum Polymers 0.000 description 2
- 229940082509 xanthan gum Drugs 0.000 description 2
- WUMLGKYWNVOBHZ-GWZLKKCNSA-N (2S,3R,4R,5R,6R)-2-bromo-3-chloro-3,4,5,6-tetrahydroxy-4-(1H-indol-2-yl)oxane-2-carboxylate cyclohexylazanium Chemical compound [NH3+]C1CCCCC1.O[C@@H]1O[C@](Br)(C([O-])=O)[C@](O)(Cl)[C@](O)([C@H]1O)c1cc2ccccc2[nH]1 WUMLGKYWNVOBHZ-GWZLKKCNSA-N 0.000 description 1
- XVARCVCWNFACQC-MBJXGIAVSA-N (2r,3r,4s,5r,6s)-2-(hydroxymethyl)-6-(1h-indol-3-yloxy)oxane-3,4,5-triol Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1OC1=CNC2=CC=CC=C12 XVARCVCWNFACQC-MBJXGIAVSA-N 0.000 description 1
- LXVCKAHHHVVOJV-KQXJFYCDSA-N (2s,3r,4s,5r)-2-[(5-bromo-4-chloro-1h-indol-3-yl)oxy]oxane-3,4,5-triol Chemical compound O[C@@H]1[C@@H](O)[C@H](O)CO[C@H]1OC1=CNC2=CC=C(Br)C(Cl)=C12 LXVCKAHHHVVOJV-KQXJFYCDSA-N 0.000 description 1
- CHRVKCMQIZYLNM-MBJXGIAVSA-N (2s,3r,4s,5r,6r)-2-[(5-bromo-6-chloro-1h-indol-3-yl)oxy]-6-(hydroxymethyl)oxane-3,4,5-triol Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1OC1=CNC2=CC(Cl)=C(Br)C=C12 CHRVKCMQIZYLNM-MBJXGIAVSA-N 0.000 description 1
- CHRVKCMQIZYLNM-RKQHYHRCSA-N (2s,3r,4s,5s,6r)-2-[(5-bromo-6-chloro-1h-indol-3-yl)oxy]-6-(hydroxymethyl)oxane-3,4,5-triol Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1=CNC2=CC(Cl)=C(Br)C=C12 CHRVKCMQIZYLNM-RKQHYHRCSA-N 0.000 description 1
- OQWBAXBVBGNSPW-RKQHYHRCSA-N (2s,3r,4s,5s,6r)-2-[(6-chloro-1h-indol-3-yl)oxy]-6-(hydroxymethyl)oxane-3,4,5-triol Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1=CNC2=CC(Cl)=CC=C12 OQWBAXBVBGNSPW-RKQHYHRCSA-N 0.000 description 1
- UKTKOBRRRRODGL-UHFFFAOYSA-N (5-bromo-4-chloro-1h-indol-3-yl) butanoate Chemical compound C1=C(Br)C(Cl)=C2C(OC(=O)CCC)=CNC2=C1 UKTKOBRRRRODGL-UHFFFAOYSA-N 0.000 description 1
- FREDWHLFBOAXBQ-UHFFFAOYSA-N (5-bromo-4-chloro-1h-indol-3-yl) hexadecanoate Chemical compound C1=C(Br)C(Cl)=C2C(OC(=O)CCCCCCCCCCCCCCC)=CNC2=C1 FREDWHLFBOAXBQ-UHFFFAOYSA-N 0.000 description 1
- VZAORBVTZPOGMO-UHFFFAOYSA-N (5-bromo-6-chloro-1h-indol-3-yl) butanoate Chemical compound ClC1=C(Br)C=C2C(OC(=O)CCC)=CNC2=C1 VZAORBVTZPOGMO-UHFFFAOYSA-N 0.000 description 1
- CJNPEPJBIHGEMU-UHFFFAOYSA-N (5-bromo-6-chloro-1h-indol-3-yl) dihydrogen phosphate Chemical compound ClC1=C(Br)C=C2C(OP(O)(=O)O)=CNC2=C1 CJNPEPJBIHGEMU-UHFFFAOYSA-N 0.000 description 1
- MGKOVBOTMHBOLI-UHFFFAOYSA-N (5-bromo-6-chloro-1h-indol-3-yl) hexadecanoate Chemical compound ClC1=C(Br)C=C2C(OC(=O)CCCCCCCCCCCCCCC)=CNC2=C1 MGKOVBOTMHBOLI-UHFFFAOYSA-N 0.000 description 1
- HODNRFUPDHGKHG-UHFFFAOYSA-N (5-bromo-6-chloro-1h-indol-3-yl) octanoate Chemical compound ClC1=C(Br)C=C2C(OC(=O)CCCCCCC)=CNC2=C1 HODNRFUPDHGKHG-UHFFFAOYSA-N 0.000 description 1
- GOEDSAJOXJKQKC-UHFFFAOYSA-N (6-chloro-1h-indol-3-yl) butanoate Chemical compound ClC1=CC=C2C(OC(=O)CCC)=CNC2=C1 GOEDSAJOXJKQKC-UHFFFAOYSA-N 0.000 description 1
- IFQGEGPISKJALV-UHFFFAOYSA-N (6-chloro-1h-indol-3-yl) dihydrogen phosphate Chemical compound ClC1=CC=C2C(OP(O)(=O)O)=CNC2=C1 IFQGEGPISKJALV-UHFFFAOYSA-N 0.000 description 1
- GYQVOAOTWFXDDC-UHFFFAOYSA-N (6-chloro-1h-indol-3-yl) hexadecanoate Chemical compound ClC1=CC=C2C(OC(=O)CCCCCCCCCCCCCCC)=CNC2=C1 GYQVOAOTWFXDDC-UHFFFAOYSA-N 0.000 description 1
- KBOGGXOQZZEFSL-UHFFFAOYSA-N (6-chloro-1h-indol-3-yl) octanoate Chemical compound ClC1=CC=C2C(OC(=O)CCCCCCC)=CNC2=C1 KBOGGXOQZZEFSL-UHFFFAOYSA-N 0.000 description 1
- GZCWLCBFPRFLKL-UHFFFAOYSA-N 1-prop-2-ynoxypropan-2-ol Chemical compound CC(O)COCC#C GZCWLCBFPRFLKL-UHFFFAOYSA-N 0.000 description 1
- ZXIRKECXSYACFD-UHFFFAOYSA-N 1h-indol-2-yl dihydrogen phosphate Chemical compound C1=CC=C2NC(OP(O)(=O)O)=CC2=C1 ZXIRKECXSYACFD-UHFFFAOYSA-N 0.000 description 1
- JTNGEYANGCBZLK-UHFFFAOYSA-N 1h-indol-3-yl dihydrogen phosphate Chemical compound C1=CC=C2C(OP(O)(=O)O)=CNC2=C1 JTNGEYANGCBZLK-UHFFFAOYSA-N 0.000 description 1
- DVFSEXUMYANGAN-UHFFFAOYSA-N 1h-indol-3-yl dihydrogen phosphate;4-methylaniline Chemical compound CC1=CC=C(N)C=C1.C1=CC=C2C(OP(O)(=O)O)=CNC2=C1 DVFSEXUMYANGAN-UHFFFAOYSA-N 0.000 description 1
- SMZOUWXMTYCWNB-UHFFFAOYSA-N 2-(2-methoxy-5-methylphenyl)ethanamine Chemical compound COC1=CC=C(C)C=C1CCN SMZOUWXMTYCWNB-UHFFFAOYSA-N 0.000 description 1
- NIXOWILDQLNWCW-UHFFFAOYSA-N 2-Propenoic acid Natural products OC(=O)C=C NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 description 1
- JYYLQSCZISREGY-UHFFFAOYSA-N 2-amino-4-chlorobenzoic acid Chemical compound NC1=CC(Cl)=CC=C1C(O)=O JYYLQSCZISREGY-UHFFFAOYSA-N 0.000 description 1
- SUWPNTKTZYIFQT-XZINFULNSA-N 5-bromo-4-chloro-3-indolyl N-acetyl-beta-D-glucosaminide Chemical compound CC(=O)N[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1=CNC2=CC=C(Br)C(Cl)=C12 SUWPNTKTZYIFQT-XZINFULNSA-N 0.000 description 1
- OPIFSICVWOWJMJ-LNNRFACYSA-N 5-bromo-4-chloro-3-indolyl beta-D-glucoside Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1=CNC2=CC=C(Br)C(Cl)=C12 OPIFSICVWOWJMJ-LNNRFACYSA-N 0.000 description 1
- QTWMXGHFXBQNEJ-UHFFFAOYSA-N 5-bromo-4-chloro-3-indolyl octanoate Chemical compound C1=C(Br)C(Cl)=C2C(OC(=O)CCCCCCC)=CNC2=C1 QTWMXGHFXBQNEJ-UHFFFAOYSA-N 0.000 description 1
- 108010051457 Acid Phosphatase Proteins 0.000 description 1
- 102000013563 Acid Phosphatase Human genes 0.000 description 1
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 description 1
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 1
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 1
- 101100309712 Arabidopsis thaliana SD11 gene Proteins 0.000 description 1
- 241000972773 Aulopiformes Species 0.000 description 1
- 108010023063 Bacto-peptone Proteins 0.000 description 1
- VLKSJYFGXZQSDX-UHFFFAOYSA-N BrC1=CC=C2NC(OP(O)(=O)O)=CC2=C1Cl Chemical compound BrC1=CC=C2NC(OP(O)(=O)O)=CC2=C1Cl VLKSJYFGXZQSDX-UHFFFAOYSA-N 0.000 description 1
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
- 229920002134 Carboxymethyl cellulose Polymers 0.000 description 1
- 241000588923 Citrobacter Species 0.000 description 1
- 241000702221 Cystoviridae Species 0.000 description 1
- 241000305071 Enterobacterales Species 0.000 description 1
- 241000709744 Enterobacterio phage MS2 Species 0.000 description 1
- 241000194032 Enterococcus faecalis Species 0.000 description 1
- 241000588722 Escherichia Species 0.000 description 1
- 241000644323 Escherichia coli C Species 0.000 description 1
- 241000702315 Escherichia virus phiX174 Species 0.000 description 1
- 241000711549 Hepacivirus C Species 0.000 description 1
- 241000700721 Hepatitis B virus Species 0.000 description 1
- 239000004354 Hydroxyethyl cellulose Substances 0.000 description 1
- 229920000663 Hydroxyethyl cellulose Polymers 0.000 description 1
- 241000702394 Inoviridae Species 0.000 description 1
- 241000588748 Klebsiella Species 0.000 description 1
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
- 241000714210 Leviviridae Species 0.000 description 1
- 229920000161 Locust bean gum Polymers 0.000 description 1
- 229920000715 Mucilage Polymers 0.000 description 1
- 101001094044 Mus musculus Solute carrier family 26 member 6 Proteins 0.000 description 1
- 241000701553 Myoviridae Species 0.000 description 1
- AFBPFSWMIHJQDM-UHFFFAOYSA-N N-methyl-N-phenylamine Natural products CNC1=CC=CC=C1 AFBPFSWMIHJQDM-UHFFFAOYSA-N 0.000 description 1
- 229920002274 Nalgene Polymers 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- 102000045595 Phosphoprotein Phosphatases Human genes 0.000 description 1
- 241000702072 Podoviridae Species 0.000 description 1
- 241000589516 Pseudomonas Species 0.000 description 1
- GLNADSQYFUSGOU-GPTZEZBUSA-J Trypan blue Chemical compound [Na+].[Na+].[Na+].[Na+].C1=C(S([O-])(=O)=O)C=C2C=C(S([O-])(=O)=O)C(/N=N/C3=CC=C(C=C3C)C=3C=C(C(=CC=3)\N=N\C=3C(=CC4=CC(=CC(N)=C4C=3O)S([O-])(=O)=O)S([O-])(=O)=O)C)=C(O)C2=C1N GLNADSQYFUSGOU-GPTZEZBUSA-J 0.000 description 1
- 241000607734 Yersinia <bacteria> Species 0.000 description 1
- FZQSLXQPHPOTHG-UHFFFAOYSA-N [K+].[K+].O1B([O-])OB2OB([O-])OB1O2 Chemical compound [K+].[K+].O1B([O-])OB2OB([O-])OB1O2 FZQSLXQPHPOTHG-UHFFFAOYSA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 241001148470 aerobic bacillus Species 0.000 description 1
- 235000010443 alginic acid Nutrition 0.000 description 1
- 229920000615 alginic acid Polymers 0.000 description 1
- 108010028144 alpha-Glucosidases Proteins 0.000 description 1
- AZDRQVAHHNSJOQ-UHFFFAOYSA-N alumane Chemical class [AlH3] AZDRQVAHHNSJOQ-UHFFFAOYSA-N 0.000 description 1
- 230000000712 assembly Effects 0.000 description 1
- 238000000429 assembly Methods 0.000 description 1
- 230000008953 bacterial degradation Effects 0.000 description 1
- 239000013602 bacteriophage vector Substances 0.000 description 1
- 102000006995 beta-Glucosidase Human genes 0.000 description 1
- 108010047754 beta-Glucosidase Proteins 0.000 description 1
- 244000078885 bloodborne pathogen Species 0.000 description 1
- 244000309464 bull Species 0.000 description 1
- 239000001110 calcium chloride Substances 0.000 description 1
- 229910001628 calcium chloride Inorganic materials 0.000 description 1
- 159000000007 calcium salts Chemical class 0.000 description 1
- 239000001768 carboxy methyl cellulose Substances 0.000 description 1
- 235000010948 carboxy methyl cellulose Nutrition 0.000 description 1
- 239000008112 carboxymethyl-cellulose Substances 0.000 description 1
- 239000012876 carrier material Substances 0.000 description 1
- 238000005266 casting Methods 0.000 description 1
- 150000001768 cations Chemical class 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000018044 dehydration Effects 0.000 description 1
- 238000006297 dehydration reaction Methods 0.000 description 1
- WCSKWBKPKXJWEG-UHFFFAOYSA-L disodium;1h-indol-3-yl phosphate Chemical compound [Na+].[Na+].C1=CC=C2C(OP([O-])(=O)[O-])=CNC2=C1 WCSKWBKPKXJWEG-UHFFFAOYSA-L 0.000 description 1
- 239000012154 double-distilled water Substances 0.000 description 1
- 229940032049 enterococcus faecalis Drugs 0.000 description 1
- 239000003248 enzyme activator Substances 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 239000011888 foil Substances 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 239000000417 fungicide Substances 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 229940097043 glucuronic acid Drugs 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 230000001744 histochemical effect Effects 0.000 description 1
- 238000000265 homogenisation Methods 0.000 description 1
- 150000004677 hydrates Chemical class 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- 235000019447 hydroxyethyl cellulose Nutrition 0.000 description 1
- 230000000415 inactivating effect Effects 0.000 description 1
- XVARCVCWNFACQC-RKQHYHRCSA-N indican Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1=CNC2=CC=CC=C12 XVARCVCWNFACQC-RKQHYHRCSA-N 0.000 description 1
- 125000001041 indolyl group Chemical group 0.000 description 1
- 230000002458 infectious effect Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- KUYNOZVWCFXSNE-BYNIDDHOSA-N inodxyl glucuronide Chemical compound O1[C@H](C(O)=O)[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1OC1=CNC2=CC=CC=C12 KUYNOZVWCFXSNE-BYNIDDHOSA-N 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 235000010420 locust bean gum Nutrition 0.000 description 1
- 239000000711 locust bean gum Substances 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000001823 molecular biology technique Methods 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 239000013642 negative control Substances 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 230000003472 neutralizing effect Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 150000004995 p-toluidines Chemical class 0.000 description 1
- RZXMPPFPUUCRFN-UHFFFAOYSA-N para-methylaniline Natural products CC1=CC=C(N)C=C1 RZXMPPFPUUCRFN-UHFFFAOYSA-N 0.000 description 1
- 239000011236 particulate material Substances 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 239000007793 ph indicator Substances 0.000 description 1
- 230000007505 plaque formation Effects 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 229920000139 polyethylene terephthalate Polymers 0.000 description 1
- 239000005020 polyethylene terephthalate Substances 0.000 description 1
- 239000003505 polymerization initiator Substances 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 1
- 229920000053 polysorbate 80 Polymers 0.000 description 1
- 229920006327 polystyrene foam Polymers 0.000 description 1
- 239000013641 positive control Substances 0.000 description 1
- XAEFZNCEHLXOMS-UHFFFAOYSA-M potassium benzoate Chemical compound [K+].[O-]C(=O)C1=CC=CC=C1 XAEFZNCEHLXOMS-UHFFFAOYSA-M 0.000 description 1
- MDAWATNFDJIBBD-UHFFFAOYSA-M potassium;1h-indol-3-yl sulfate Chemical compound [K+].C1=CC=C2C(OS(=O)(=O)[O-])=CNC2=C1 MDAWATNFDJIBBD-UHFFFAOYSA-M 0.000 description 1
- 238000007639 printing Methods 0.000 description 1
- 239000011541 reaction mixture Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000010020 roller printing Methods 0.000 description 1
- 235000019515 salmon Nutrition 0.000 description 1
- 239000000565 sealant Substances 0.000 description 1
- 238000013207 serial dilution Methods 0.000 description 1
- 229920002379 silicone rubber Polymers 0.000 description 1
- 239000004945 silicone rubber Substances 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 159000000000 sodium salts Chemical class 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 238000007655 standard test method Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 230000000699 topical effect Effects 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 238000013518 transcription Methods 0.000 description 1
- 230000035897 transcription Effects 0.000 description 1
- 239000012137 tryptone Substances 0.000 description 1
- 239000013026 undiluted sample Substances 0.000 description 1
- 241001148471 unidentified anaerobic bacterium Species 0.000 description 1
- AODQPPLFAXTBJS-UHFFFAOYSA-M victoria blue 4R Chemical compound [Cl-].C1=CC(N(C)C)=CC=C1C(C=1C=CC(=CC=1)N(C)C)=C(C=C1)C2=CC=CC=C2C1=[N+](C)C1=CC=CC=C1 AODQPPLFAXTBJS-UHFFFAOYSA-M 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
- 238000009736 wetting Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2334/00—O-linked chromogens for determinations of hydrolase enzymes, e.g. glycosidases, phosphatases, esterases
- C12Q2334/50—Indoles
- C12Q2334/52—5-Bromo-4-chloro-3-indolyl, i.e. BCI
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Toxicology (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US08/844,145 US5958675A (en) | 1997-04-18 | 1997-04-18 | Method for detecting bacteria using bacteriophage, contrast-coloring dye and precipitable dye |
| US844145 | 1997-04-18 | ||
| PCT/US1997/014965 WO1998048042A2 (en) | 1997-04-18 | 1997-08-26 | Methods and devices for detecting bacteriophage |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE69732255D1 DE69732255D1 (de) | 2005-02-17 |
| DE69732255T2 true DE69732255T2 (de) | 2006-01-12 |
Family
ID=25291942
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE69732255T Expired - Fee Related DE69732255T2 (de) | 1997-04-18 | 1997-08-26 | Verfahren und vorrichtungen zum nachweis von bakteriophagen |
Country Status (7)
| Country | Link |
|---|---|
| US (2) | US5958675A (enExample) |
| EP (1) | EP0983375B1 (enExample) |
| JP (1) | JP2002513288A (enExample) |
| AU (1) | AU4162097A (enExample) |
| CA (1) | CA2286463A1 (enExample) |
| DE (1) | DE69732255T2 (enExample) |
| WO (1) | WO1998048042A2 (enExample) |
Families Citing this family (65)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5958675A (en) * | 1997-04-18 | 1999-09-28 | 3M Innovative Properties Company | Method for detecting bacteria using bacteriophage, contrast-coloring dye and precipitable dye |
| US6984499B2 (en) * | 1997-10-02 | 2006-01-10 | Idexx Laboratories, Inc. | Method and apparatus for concurrently detecting pathogenic organisms and antimicrobial susceptibility |
| DE60014987T2 (de) * | 1999-02-22 | 2005-11-10 | Matsushita Electric Industrial Co., Ltd., Kadoma | Verfahren zum Nachweis von Bakterien |
| AU781090B2 (en) * | 1999-07-30 | 2005-05-05 | Biomerieux S.A. | Detection and identification of bacterial strains |
| US6391626B1 (en) | 1999-08-23 | 2002-05-21 | 3M Innovative Properties Company | Culture medium and device using ballasted pH indicators for detection and enumeration of microorganisms |
| US6737266B1 (en) * | 1999-10-01 | 2004-05-18 | 3M Innovative Properties Company | Devices and methods for microorganism detection |
| US6649406B1 (en) * | 1999-11-23 | 2003-11-18 | 3M Innovative Properties Company | Device for propagation and storage of microorganisms |
| US6436661B1 (en) * | 2000-04-13 | 2002-08-20 | 3M Innovative Properties Company | Bacteria and bacteriophage detection using immobilized enzyme substrates |
| US6461608B1 (en) | 2000-11-22 | 2002-10-08 | Nymox Pharmaceutical Corporation | Bacteriophage composition useful in treating food products to prevent bacterial contamination |
| WO2002061117A1 (de) * | 2001-02-01 | 2002-08-08 | Profos Ag | Nachweis und identifikation von bakteriengruppen |
| US20020192676A1 (en) * | 2001-06-18 | 2002-12-19 | Madonna Angelo J. | Method for determining if a type of bacteria is present in a mixture |
| FR2833613B1 (fr) * | 2001-12-13 | 2004-08-27 | Alain Rambach | Procede de detection de microorganismes |
| US7384778B2 (en) * | 2002-01-10 | 2008-06-10 | Idexx Laboratories, Inc. | Methods and devices for the detection of pathogenic microorganisms and their antimicrobial susceptibility |
| ATE469240T1 (de) * | 2002-04-12 | 2010-06-15 | Colorado School Of Mines | Verfahren zum nachweis geringer konzentrationen eines zielbakteriums unter verwendung von phagen zur infizierung von zielbakterienzellen |
| US8216780B2 (en) * | 2002-04-12 | 2012-07-10 | Microphage (Tm) Incorporated | Method for enhanced sensitivity in bacteriophage-based diagnostic assays |
| US20050003346A1 (en) * | 2002-04-12 | 2005-01-06 | Colorado School Of Mines | Apparatus and method for detecting microscopic living organisms using bacteriophage |
| RU2245368C2 (ru) * | 2002-11-14 | 2005-01-27 | Домарадский Игорь Валерианович | Способ индикации микроорганизмов |
| JP4764972B2 (ja) * | 2003-12-03 | 2011-09-07 | 大学共同利用機関法人情報・システム研究機構 | マルチウェルプレート |
| US7399608B2 (en) * | 2003-12-16 | 2008-07-15 | Kimberly-Clark Worldwide, Inc. | Microbial detection and quantification |
| US7282349B2 (en) | 2003-12-16 | 2007-10-16 | Kimberly-Clark Worldwide, Inc. | Solvatochromatic bacterial detection |
| US7300770B2 (en) * | 2004-12-16 | 2007-11-27 | Kimberly-Clark Worldwide, Inc. | Detection of microbe contamination on elastomeric articles |
| WO2008157384A2 (en) | 2007-06-15 | 2008-12-24 | Microphage Incorporated | Method of detection of microorganisms with enhanced bacteriophage amplification |
| US20050239200A1 (en) * | 2004-04-23 | 2005-10-27 | Beckwith Scott W | Devices for culturing anaerobic microorganisms and methods of using the same |
| US7521201B2 (en) * | 2004-11-01 | 2009-04-21 | Strategic Diagnostics Inc. | Bacteriophages as selective agents |
| GB0427050D0 (en) * | 2004-12-10 | 2005-01-12 | Amersham Biosciences Uk Ltd | Method of,and apparatus and computer software for,imaging biological objects |
| GB0503172D0 (en) | 2005-02-16 | 2005-03-23 | Enigma Diagnostics Ltd | Detection method |
| US20110097702A1 (en) * | 2005-03-31 | 2011-04-28 | Voorhees Kent J | Methods and compositions for in situ detection of microorganisms on a surface |
| WO2006105504A1 (en) * | 2005-03-31 | 2006-10-05 | Microphage Incorporated | Apparatus and method for detecting microorganisms using flagged bacteriophage |
| JP4729327B2 (ja) * | 2005-04-04 | 2011-07-20 | 株式会社日研生物医学研究所 | 環境微生物検査用具 |
| US7727513B2 (en) * | 2005-12-15 | 2010-06-01 | Kimberly-Clark Worldwide, Inc. | Method for screening for bacterial conjunctivitis |
| US20070178450A1 (en) * | 2006-01-27 | 2007-08-02 | Microphage (Tm) Incorporation | Method and apparatus for determining level of microorganisms using bacteriophage |
| US8377678B2 (en) * | 2006-07-27 | 2013-02-19 | Micrology Methods, Llc | Methodology for detection, enumeration, propagation and manipulation of bacteriophages |
| US7531319B2 (en) * | 2006-08-31 | 2009-05-12 | Kimberly-Clark Worldwide, Inc. | Array for rapid detection of a microorganism |
| US20080057534A1 (en) * | 2006-08-31 | 2008-03-06 | Kimberly-Clark Worldwide, Inc. | Microbe-sensitive indicators and use of the same |
| US7763442B2 (en) | 2006-08-31 | 2010-07-27 | Kimberly-Clark Worldwide, Inc. | Method for detecting candida on skin |
| WO2008063838A2 (en) * | 2006-10-31 | 2008-05-29 | Microphage Incorporated | Method and apparatus for enhanced bacteriophage-based diagnostic assays by selective inhibition of potential cross-reactive organisms |
| EP2135083B1 (en) * | 2007-02-12 | 2013-10-23 | Charm Sciences Inc. | Method for phage detection |
| US9376704B2 (en) * | 2007-02-12 | 2016-06-28 | Charm Sciences, Inc. | Test kit and method for detecting bacteriophage |
| ES2446943T5 (es) * | 2007-08-31 | 2017-03-14 | Ssi Diagnostica A/S | Composiciones y medios para diagnosticar infecciones microbianas |
| EP2209906A2 (en) * | 2007-11-05 | 2010-07-28 | Life Technologies Corporation | Novel plating media |
| US20090142275A1 (en) * | 2007-11-29 | 2009-06-04 | Kimberly-Clark Worldwide, Inc. | Wound Suture Capable of Identifying the Presence of Bacteria |
| US8697434B2 (en) * | 2008-01-11 | 2014-04-15 | Colorado School Of Mines | Detection of phage amplification by SERS nanoparticles |
| US9441204B2 (en) * | 2008-04-03 | 2016-09-13 | Colorado School Of Mines | Compositions and methods for detecting Yersinia pestis bacteria |
| US8349580B2 (en) | 2009-09-09 | 2013-01-08 | 3M Innovative Properties Company | Methods and kit for protease enzyme assays |
| MX2012007557A (es) * | 2009-12-31 | 2014-01-31 | Bio Rad Innovations | Un medio de cultivo para seleccion o enriquecimiento de s. aureus resistente a meticilina. |
| FR2983214B1 (fr) * | 2011-11-28 | 2015-01-16 | Alain Rambach | Milieu et procede de detection de bacteries yersinia enterocolitica pathogenes |
| US8921067B2 (en) * | 2013-02-04 | 2014-12-30 | 3M Innovative Properties Company | Method and culture device for detecting yeasts and molds |
| WO2014129460A1 (ja) * | 2013-02-19 | 2014-08-28 | ユニチカ株式会社 | インドキシル硫酸の測定方法 |
| US9433239B2 (en) | 2013-03-15 | 2016-09-06 | Altria Client Services Llc | Bacteriophage and methods of making and using |
| JP2015029441A (ja) * | 2013-07-31 | 2015-02-16 | 大日本印刷株式会社 | 微生物培養シート |
| PT3068894T (pt) * | 2013-11-13 | 2019-07-19 | Univ Barcelona | Método para detecção de condições bacteriolíticas numa amostra |
| EP3553163A1 (en) * | 2014-03-07 | 2019-10-16 | 3M Innovative Properties Company | Article and method for detecting aerobic bacteria |
| US20170268031A1 (en) * | 2014-07-30 | 2017-09-21 | The Regents Of The University Of California | Methods for bacteriophage detection |
| WO2016123425A1 (en) | 2015-01-29 | 2016-08-04 | Altria Client Services Llc | Endolysin from bacteriophage against geobacillus and methods of using |
| WO2016123427A1 (en) | 2015-01-29 | 2016-08-04 | Altria Client Services Llc | Bacteriophage and methods of using |
| CN107532126A (zh) * | 2015-04-29 | 2018-01-02 | 3M创新有限公司 | 具有二氧化碳发生剂的薄膜培养装置 |
| WO2017013673A1 (en) | 2015-07-21 | 2017-01-26 | M/S Xcellence In Bio Innovation And Technologies Pvt. Ltd. | An invention relating to microbiological testing apparatus |
| US10563164B1 (en) | 2015-10-08 | 2020-02-18 | Charm Sciences, Inc. | Plate reader |
| US10495563B1 (en) | 2016-04-28 | 2019-12-03 | Charm Sciences, Inc. | Plate reader observation methods and operation |
| US20190316169A1 (en) * | 2016-11-28 | 2019-10-17 | Sonanutech Inc. | Methods, compositions, and kits for detecting a cell in a sample |
| EP3607081A1 (en) | 2017-04-03 | 2020-02-12 | 3M Innovative Properties Company | Rapid detection of e. coli in a thin film culture device |
| JP7125975B2 (ja) * | 2017-04-03 | 2022-08-25 | スリーエム イノベイティブ プロパティズ カンパニー | 薄膜培養デバイスにおける大腸菌の迅速な検出 |
| FR3074047B1 (fr) * | 2017-11-30 | 2019-12-20 | Biomerieux | Dispositifs medicaux non-implantables integrant un dispositif de detection et/ou d'identification d'infections microbiologiques |
| FR3074046B1 (fr) * | 2017-11-30 | 2020-11-20 | Biomerieux Sa | Dispositifs de detection et/ou d'identification d'infections microbiologiques, pour dispositifs medicaux non-implantables |
| IT202000022519A1 (it) * | 2020-09-24 | 2022-03-24 | Craniomed Group Srl | "metodica innovativa per il rilevamento dei virus che possono essere dei fagi o avere un meccanismo fagico, incluso sars cov-2 e congeneri, e che interessano la specie umana, il regno l’animale, il regno vegetale, e gli altri regni". |
Family Cites Families (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4565783A (en) * | 1981-01-27 | 1986-01-21 | Minnesota Mining And Manufacturing Company | Dry culture media |
| US4797363A (en) * | 1984-03-19 | 1989-01-10 | Board Of Trustees, University Of Illinois | Bacteriophages as recognition and identification agents |
| JP2944694B2 (ja) * | 1988-10-04 | 1999-09-06 | ディーエヌエー プラント テクノロジー コーポレイション | 検出可能な表現型のファージ形質導入による細菌の検出 |
| JPH02110000A (ja) * | 1988-10-19 | 1990-04-23 | Mitsubishi Heavy Ind Ltd | バクテリオフアージの検出法 |
| US5089413A (en) * | 1989-05-19 | 1992-02-18 | Minnesota Mining And Manufacturing Company | Method and apparatus for culturing with microbiological dry culture medium |
| CA2022614A1 (en) * | 1989-08-31 | 1991-03-01 | Richard R. Matner | Colony blotting method and device |
| JPH03133399A (ja) * | 1989-10-20 | 1991-06-06 | Mitsubishi Heavy Ind Ltd | バクテリオファージの検出方法 |
| US5210022A (en) * | 1990-04-20 | 1993-05-11 | Rcr Scientific, Inc. | Method test media and chromogenic compounds for identifying and differentiating general coliforms and Escherichia coli bacteria |
| GB9017443D0 (en) * | 1990-08-09 | 1990-09-26 | Amersham Int Plc | Reporter bacteria for rapid microbial detection |
| US5232838A (en) * | 1991-12-09 | 1993-08-03 | Minnesota Mining And Manufacturing Company | Culture media device and method of use |
| US5409838A (en) * | 1992-12-23 | 1995-04-25 | Minnesota Mining And Manufacturing Company | Use of acid to stabilize indicator dyes in acrylate adhesives |
| US5364766A (en) * | 1993-05-14 | 1994-11-15 | Minnesota Mining And Manufacturing Company | Culture medium for rapid count of coliform bacteria |
| US5527667A (en) * | 1993-05-25 | 1996-06-18 | Virginia Polytechnic Institute And State University | Water sample viral contamination detection system |
| GB9317139D0 (en) * | 1993-08-18 | 1993-10-06 | Miniser For Agriculture Fisher | Method and test kits for detection of bacteriophage |
| US5443963A (en) * | 1994-01-31 | 1995-08-22 | Minnesota Mining And Manufacturing Company | Method for detecting staphylococci |
| EP0744896B1 (en) * | 1994-02-17 | 1998-08-12 | MERCK PATENT GmbH | Antiviral or antifungal composition and method |
| US5462860A (en) * | 1994-06-06 | 1995-10-31 | Minnesota Mining And Manufacturing Company | Conditioned culture medium for rapid growth and detection of microbes |
| US5958675A (en) * | 1997-04-18 | 1999-09-28 | 3M Innovative Properties Company | Method for detecting bacteria using bacteriophage, contrast-coloring dye and precipitable dye |
-
1997
- 1997-04-18 US US08/844,145 patent/US5958675A/en not_active Expired - Lifetime
- 1997-08-26 CA CA002286463A patent/CA2286463A1/en not_active Abandoned
- 1997-08-26 JP JP54566098A patent/JP2002513288A/ja active Pending
- 1997-08-26 EP EP97939554A patent/EP0983375B1/en not_active Expired - Lifetime
- 1997-08-26 DE DE69732255T patent/DE69732255T2/de not_active Expired - Fee Related
- 1997-08-26 AU AU41620/97A patent/AU4162097A/en not_active Abandoned
- 1997-08-26 WO PCT/US1997/014965 patent/WO1998048042A2/en not_active Ceased
-
1999
- 1999-07-09 US US09/350,978 patent/US6090541A/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| EP0983375B1 (en) | 2005-01-12 |
| CA2286463A1 (en) | 1998-10-29 |
| WO1998048042A2 (en) | 1998-10-29 |
| US6090541A (en) | 2000-07-18 |
| AU4162097A (en) | 1998-11-13 |
| EP0983375A1 (en) | 2000-03-08 |
| JP2002513288A (ja) | 2002-05-08 |
| US5958675A (en) | 1999-09-28 |
| DE69732255D1 (de) | 2005-02-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| EP0983375B1 (en) | Methods and devices for detecting bacteriophage | |
| DE60015595T2 (de) | Gerät zur aufbewahrung und vermehrung von mikroorganismen | |
| DE69508921T2 (de) | Verfahren zum nachweis von staphylokokken | |
| DE69207430T2 (de) | Kulturmedienvorrichtung | |
| DE69515660T2 (de) | Konditioniertes kulturmedium zu schnellem wachstum und nachweis von mikroorganismen | |
| DE69534301T3 (de) | Verfahren zum nachweis und zur quantifizierung von enterobakteriaceae | |
| DE69618954T2 (de) | Zählvorrichtung für oberflächenkolonien sowie anwendungsverfahren | |
| DE69413026T2 (de) | Nährmedium für die rasche zählung von coliformen bakterien | |
| JP2020072687A (ja) | 好気性菌を検出するための物品及び方法 | |
| DE69814361T2 (de) | Identifikation von salmonella | |
| EP3607083B1 (en) | Rapid detection of e. coli in a thin film culture device | |
| US12624379B2 (en) | Rapid detection of E. coli in a thin film culture device | |
| JP3115002B2 (ja) | 微生物培地 | |
| CH615460A5 (enExample) | ||
| JP3850465B2 (ja) | 簡易培地及び微生物の検出方法 | |
| US11851643B2 (en) | Selective thin-film culture device for enumerating microorganisms | |
| Bennett et al. | Identification of fusobacteria in a routine diagnostic laboratory | |
| JP2006025608A (ja) | 微生物培地 | |
| JP3442865B2 (ja) | フィルム状培地及びこれを用いた微生物の検出方法 | |
| Brown et al. | Pseudo-colonies simulating those of pleuropneumonia-like microorganisms | |
| US3597321A (en) | Diagnostic compostion for the differentiation of staphylococci | |
| DE69518527T2 (de) | Kulturmedium für schnellzählung koliformer bakterien | |
| DE2518282A1 (de) | Vorrichtung zum zuechten und nachweis von mikroorganismen | |
| DE69512263T2 (de) | Assay für prolin-iminopeptidase und andere hydrolytische aktivitäten | |
| DE2914544A1 (de) | Testmittel zum nachweis der desoxyribonuclease-bildung, verfahren zur herstellung derselben, sowie dessen anwendung |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8364 | No opposition during term of opposition | ||
| 8339 | Ceased/non-payment of the annual fee |