DE69730145D1 - VERFAHREN ZUM AUFFINDEN, NACHWEISEN, ANREICHERN UND/ODER ISOLIEREN VON ZIEL-NUcLEINSÄURESEQUENZEN UNTER VERWENDUNG VON VON RECA ÄHNLICHER REKOMBINASE - Google Patents

VERFAHREN ZUM AUFFINDEN, NACHWEISEN, ANREICHERN UND/ODER ISOLIEREN VON ZIEL-NUcLEINSÄURESEQUENZEN UNTER VERWENDUNG VON VON RECA ÄHNLICHER REKOMBINASE

Info

Publication number
DE69730145D1
DE69730145D1 DE69730145T DE69730145T DE69730145D1 DE 69730145 D1 DE69730145 D1 DE 69730145D1 DE 69730145 T DE69730145 T DE 69730145T DE 69730145 T DE69730145 T DE 69730145T DE 69730145 D1 DE69730145 D1 DE 69730145D1
Authority
DE
Germany
Prior art keywords
reca
recombinase
enriching
detecting
nucleic acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69730145T
Other languages
English (en)
Other versions
DE69730145T2 (de
Inventor
Koji Kigawa
Mikayo Yamanaka
Kayo Kusumi
Eli Mukai
Kazuaki Obata
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Tapestry Pharmaceuticals Inc
Original Assignee
Tapestry Pharmaceuticals Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Tapestry Pharmaceuticals Inc filed Critical Tapestry Pharmaceuticals Inc
Application granted granted Critical
Publication of DE69730145D1 publication Critical patent/DE69730145D1/de
Publication of DE69730145T2 publication Critical patent/DE69730145T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6839—Triple helix formation or other higher order conformations in hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation
DE69730145T 1996-08-29 1997-08-29 VERFAHREN ZUM AUFFINDEN, NACHWEISEN, ANREICHERN UND/ODER ISOLIEREN VON ZIEL-NUcLEINSÄURESEQUENZEN UNTER VERWENDUNG VON VON RECA ÄHNLICHER REKOMBINASE Expired - Fee Related DE69730145T2 (de)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
JP22906196 1996-08-29
JP22906196 1996-08-29
JP34709096 1996-12-26
JP34709096 1996-12-26
PCT/JP1997/003019 WO1998008975A1 (en) 1996-08-29 1997-08-29 Methods for targeting, enriching, detecting and/or isolating target nucleic acid sequence using reca-like recombinase

Publications (2)

Publication Number Publication Date
DE69730145D1 true DE69730145D1 (de) 2004-09-09
DE69730145T2 DE69730145T2 (de) 2005-07-14

Family

ID=26528611

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69730145T Expired - Fee Related DE69730145T2 (de) 1996-08-29 1997-08-29 VERFAHREN ZUM AUFFINDEN, NACHWEISEN, ANREICHERN UND/ODER ISOLIEREN VON ZIEL-NUcLEINSÄURESEQUENZEN UNTER VERWENDUNG VON VON RECA ÄHNLICHER REKOMBINASE

Country Status (7)

Country Link
US (1) US6335164B1 (de)
EP (1) EP0939834B1 (de)
AT (1) ATE272720T1 (de)
AU (1) AU712873B2 (de)
CA (1) CA2263815A1 (de)
DE (1) DE69730145T2 (de)
WO (1) WO1998008975A1 (de)

Families Citing this family (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3065035B2 (ja) * 1998-10-01 2000-07-12 ダイキン工業株式会社 高性能なＲｅｃＡ様組換え酵素／１本鎖核酸プローブ複合体の調製方法及びその利用
JP4509307B2 (ja) * 2000-06-07 2010-07-21 株式会社アイシン・コスモス研究所 ＲｅｃＡタンパク質を用いてライブラリーを加工する方法
JP4616988B2 (ja) * 2000-12-20 2011-01-19 株式会社アイシン・コスモス研究所 三本鎖ｄｎａ形成技術を応用したｄｎａ多型検出方法
EP1414840A4 (de) * 2001-03-27 2005-04-13 Univ Delaware Genomics-anwendungen für modifizierte oligonukleotide
JP5128031B2 (ja) * 2001-03-28 2013-01-23 株式会社アイシン・コスモス研究所 ＲｅｃＡタンパク質を用いて標識が導入された核酸を製造する方法
CA2444649C (en) 2001-04-20 2012-10-02 The Penn State Research Foundation Methods for nucleic acid manipulation
US7468244B2 (en) * 2001-09-27 2008-12-23 University Of Delaware Polymorphism detection and separation
WO2003027640A2 (en) * 2001-09-28 2003-04-03 University Of Delaware Polymorphism detection and separation
KR100468624B1 (ko) * 2002-01-03 2005-01-27 한국생명공학연구원 미생물 리컴비네이즈를 이용한 색소체 형질전환 방법
ATE400663T1 (de) 2002-02-21 2008-07-15 Asm Scient Inc Rekombinase-polymerase-amplifikation
US8030000B2 (en) 2002-02-21 2011-10-04 Alere San Diego, Inc. Recombinase polymerase amplification
US7399590B2 (en) 2002-02-21 2008-07-15 Asm Scientific, Inc. Recombinase polymerase amplification
US7759057B2 (en) * 2004-02-04 2010-07-20 Ut-Battelle, Llc Method for analyzing microbial communities
US20050260624A1 (en) * 2004-02-28 2005-11-24 Wang Chang N J Novel nucleic acid complexes and detection thereof
EP1929049B1 (de) 2005-07-25 2013-04-10 Alere San Diego, Inc. Verfahren zur multiplexierung einer rekombinase-polymerase-verstärkung
US8071308B2 (en) 2006-05-04 2011-12-06 Alere San Diego, Inc. Recombinase polymerase amplification
US9689031B2 (en) 2007-07-14 2017-06-27 Ionian Technologies, Inc. Nicking and extension amplification reaction for the exponential amplification of nucleic acids
JP2010017178A (ja) * 2008-06-11 2010-01-28 Sumitomo Chemical Co Ltd Ｄｎａを定量又は検出する方法
US9334531B2 (en) 2010-12-17 2016-05-10 Life Technologies Corporation Nucleic acid amplification
US9309566B2 (en) 2010-12-17 2016-04-12 Life Technologies Corporation Methods, compositions, systems, apparatuses and kits for nucleic acid amplification
US9309557B2 (en) 2010-12-17 2016-04-12 Life Technologies Corporation Nucleic acid amplification
US9469867B2 (en) 2009-05-20 2016-10-18 Alere San Diego, Inc. DNA glycosylase/lyase and AP endonuclease substrates
WO2010141940A1 (en) 2009-06-05 2010-12-09 Alere San Diego, Inc. Recombinase polymerase amplification reagents and kits
JP5907990B2 (ja) 2010-12-17 2016-05-11 ライフ テクノロジーズ コーポレーション 核酸増幅のための方法、組成物、システム、装置およびキット
JP5961247B2 (ja) 2011-04-07 2016-08-02 アリーア サン ディエゴ， インコーポレイテッド リコンビナーゼポリメラーゼ増幅混合物のモニタリング
WO2014020137A1 (en) 2012-08-02 2014-02-06 Qiagen Gmbh Recombinase mediated targeted dna enrichment for next generation sequencing
US20180282786A1 (en) * 2017-04-01 2018-10-04 Michael Joseph Pugia Methods and apparatus for selective nucleic acid separation

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4888274A (en) 1985-09-18 1989-12-19 Yale University RecA nucleoprotein filament and methods
US5223414A (en) 1990-05-07 1993-06-29 Sri International Process for nucleic acid hybridization and amplification
US5670316A (en) 1990-05-07 1997-09-23 Daikin Industries, Ltd. Diagnostic applications of double D-loop formation
US5445090A (en) 1991-07-25 1995-08-29 Mim Industries, Inc. Interchangeable clamp for use in a sewing machine
US5506098A (en) 1991-09-04 1996-04-09 Daikin Industries, Ltd. In situ hybridization method
AU4115693A (en) 1992-04-24 1993-11-29 Sri International In vivo homologous sequence targeting in eukaryotic cells
US5468629A (en) 1993-04-13 1995-11-21 Calhoun; Cornelia Method of promoting in vitro homologous recombination transfection in mammalian cells using the RecA protein
AU1283095A (en) 1993-12-28 1995-07-17 Daikin Industries, Ltd. In-situ hybridization method using reca protein and reca protein having marker or ligand for use in said method

Also Published As

Publication number Publication date
WO1998008975A1 (en) 1998-03-05
EP0939834A1 (de) 1999-09-08
ATE272720T1 (de) 2004-08-15
AU4032097A (en) 1998-03-19
DE69730145T2 (de) 2005-07-14
US6335164B1 (en) 2002-01-01
EP0939834B1 (de) 2004-08-04
CA2263815A1 (en) 1998-03-05
AU712873B2 (en) 1999-11-18

Similar Documents

Publication Publication Date Title
DE69730145D1 (de) VERFAHREN ZUM AUFFINDEN, NACHWEISEN, ANREICHERN UND/ODER ISOLIEREN VON ZIEL-NUcLEINSÄURESEQUENZEN UNTER VERWENDUNG VON VON RECA ÄHNLICHER REKOMBINASE
DE69531831D1 (de) Verfahren mit einer hohen umsatzrate für das auffinden von sequenzen oder genetischen veränderungen in nukleinsäuren
DE69333242D1 (de) Verfahren, reagenz und salz zum nachweis und amplifikation von nukleinsäuresequenzen
ATE414169T1 (de) Methoden zur detektion von nukleinsäuren unter verwendung von zymogenen und dazugehörige kits
ATE339514T1 (de) Verfahren zum nachweis und amplifikation von nukleinsäuresequenzen unter verbrauch von modifizierten oligonukleotiden mit erhöhter zielschmelztemperatur (tm)
GB2228998B (en) A method for the detection of nucleic acid sequences using fluorescence polarisation
DK0796345T3 (da) Apparat til udførelse af magnetisk cyklusreaktion
ATE255168T1 (de) Massenspektroskopische verfahren zur sequenzierung von nukleinsäuren
DE602004020220D1 (de) Mittel und verfahren zum nachweis von zielnukleotidsequenzen unter verwendung von ligationstests mit verbesserten oligonukleotidsondenpaaren
DE69938894D1 (de) Verfahren zum nachweis von ziel-nukleinsäuren mittels pcr
WO1999051774A3 (en) A method for obtaining a plant with a genetic lesion in a gene sequence
EP1055736A4 (de) Verfaheren zur dna-sequenzierung
IL71715A0 (en) Method,gene probes and test kit for detecting bacteria by nucleic acid hybridization
NZ511415A (en) Multi-loci genomic analysis by a method of improved cycle sequencing
ATE325893T1 (de) Fluorometrisches verfahren zur überwachung und zum nachweis von nucleinsäure-amplifizierung
DE59712871D1 (de) Heterocyclische verbindungen und deren verwendung in der detektion von nucleinsäuren
DE602004032114D1 (de) Rna-interferenz für das gen znfn3a1 als verfahren zur hemmung des wachstums von krebszellen
US20220002798A1 (en) Nanopore sequencing method
DE50110507D1 (de) Verfahren in vitro zum einbringen von nukleinsäure in eine zelle (transfektion) mittels calciumphosphat
CN113366120B (zh) 纳米孔测序方法
ATE220425T1 (de) Verfahren zur bestimmung einer prädisposition zu seronegativen spondyloarthropathien und produkte dafür
ATE40409T1 (de) Photoimmunologisches testverfahren fuer dns und rns.
ATE283480T1 (de) Verfahren zum nachweis von agentien die die aktivität der promotersequenz des crf-2 alpha rezeptors verändern

Legal Events

Date Code Title Description
8327 Change in the person/name/address of the patent owner

Owner name: TAPESTRY PHARMACEUTICALS, INC., BOULDER, COL., US

8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee