DE69637798D1 - Verfahren zur Reduzierung der RNA-Konzentration in einer Mischung biologischen Materials unter Verwendung von Diatomeen-Erde - Google Patents

Verfahren zur Reduzierung der RNA-Konzentration in einer Mischung biologischen Materials unter Verwendung von Diatomeen-Erde

Info

Publication number
DE69637798D1
DE69637798D1 DE69637798T DE69637798T DE69637798D1 DE 69637798 D1 DE69637798 D1 DE 69637798D1 DE 69637798 T DE69637798 T DE 69637798T DE 69637798 T DE69637798 T DE 69637798T DE 69637798 D1 DE69637798 D1 DE 69637798D1
Authority
DE
Germany
Prior art keywords
mixture
concentration
biological material
rna
diatomaceous earth
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69637798T
Other languages
English (en)
Inventor
Nancy Horn
Magda Marquet
Jennifer Meek
Gregg Budahazi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Fresh Tracks Therapeutics Inc
Original Assignee
Vical Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Vical Inc filed Critical Vical Inc
Application granted granted Critical
Publication of DE69637798D1 publication Critical patent/DE69637798D1/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/08—Reducing the nucleic acid content

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Virology (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Processing Of Solid Wastes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Treatment Of Sludge (AREA)
  • Disinfection, Sterilisation Or Deodorisation Of Air (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
DE69637798T 1995-01-13 1996-01-03 Verfahren zur Reduzierung der RNA-Konzentration in einer Mischung biologischen Materials unter Verwendung von Diatomeen-Erde Expired - Fee Related DE69637798D1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/372,236 US5576196A (en) 1995-01-13 1995-01-13 Process for reducing RNA concentration in a mixture of biological material using diatomaceous earth

Publications (1)

Publication Number Publication Date
DE69637798D1 true DE69637798D1 (de) 2009-02-12

Family

ID=23467282

Family Applications (2)

Application Number Title Priority Date Filing Date
DE69637798T Expired - Fee Related DE69637798D1 (de) 1995-01-13 1996-01-03 Verfahren zur Reduzierung der RNA-Konzentration in einer Mischung biologischen Materials unter Verwendung von Diatomeen-Erde
DE69626957T Expired - Lifetime DE69626957T2 (de) 1995-01-13 1996-01-03 Verfahren zur reduzierung der rns-konzentration in einer mischung biologischen materials unter verwendung von diatomeen-erde

Family Applications After (1)

Application Number Title Priority Date Filing Date
DE69626957T Expired - Lifetime DE69626957T2 (de) 1995-01-13 1996-01-03 Verfahren zur reduzierung der rns-konzentration in einer mischung biologischen materials unter verwendung von diatomeen-erde

Country Status (8)

Country Link
US (1) US5576196A (de)
EP (2) EP0802975B1 (de)
JP (1) JP3808506B2 (de)
AT (2) ATE419341T1 (de)
DE (2) DE69637798D1 (de)
DK (1) DK0802975T3 (de)
ES (1) ES2196140T3 (de)
WO (1) WO1996021729A1 (de)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE238321T1 (de) 1996-02-06 2003-05-15 Roche Diagnostics Gmbh Verfahren zur herstellung von gereinigter nukleinsäure und deren verwendung
FR2746412B1 (fr) * 1996-03-21 1998-06-12 Rhone Poulenc Rorer Sa Purification d'adn plasmidique de qualite pharmaceutique
US7026468B2 (en) 1996-07-19 2006-04-11 Valentis, Inc. Process and equipment for plasmid purification
US7807822B2 (en) 1996-08-01 2010-10-05 Robert Bridenbaugh Methods for purifying nucleic acids
US6268492B1 (en) * 1998-08-10 2001-07-31 Chiron Corporation Methods for purifying non-chromosomal nucleic acid molecules from cells
US6617108B1 (en) 1999-07-12 2003-09-09 Technology Licensing Co. Llc Methods and compositions for biotechnical separations using selective precipitation by compaction agents
US20020012990A1 (en) * 1999-12-22 2002-01-31 Lander Russel Jackson Process for the scaleable purification of plasmid DNA
DE10033991A1 (de) * 2000-07-12 2002-01-24 Qiagen Gmbh Verfahren zur Isolierung von Nukleinsäuren
US7208271B2 (en) 2001-11-28 2007-04-24 Applera Corporation Compositions and methods of selective nucleic acid isolation
AU2003267175A1 (en) 2002-09-13 2004-04-30 Valentis, Inc. Apparatus and method for preparative scale purification of nucleic acids
DE60325773D1 (de) * 2002-12-23 2009-02-26 Vical Inc Reinigungsverfahren für plasmid-dna
US8501402B2 (en) 2003-03-24 2013-08-06 Boehringer Ingelheim Rcv Gmbh & Co Kg Methods and devices for producing biomolecules
JP2004000922A (ja) * 2003-03-26 2004-01-08 Toyobo Co Ltd 核酸またはタンパク質抽出用シリカ粒子組成物
AU2006211216B2 (en) * 2005-01-31 2011-02-03 Merck Sharp & Dohme Llc Purification process for plasmid DNA
EP2088196A1 (de) 2008-02-08 2009-08-12 Boehringer Ingelheim RCV GmbH & Co KG Verfahren und Vorrichtungen zur Herstellung von Biomolekülen
US20110313143A1 (en) * 2008-12-15 2011-12-22 Life Technologies Corporation Nucleic acid purification apparatus and method
ES2543367T3 (es) * 2010-07-07 2015-08-18 Diagon Kft. Procedimiento para el aislamiento específico del contenido de ADN total de gérmenes bacterianos y un kit para dicho fin
GB201614050D0 (en) * 2016-08-17 2016-09-28 Glaxosmithkline Ip Dev Ltd Method for purifying viral vectors
CN110612148A (zh) * 2017-06-27 2019-12-24 大金工业株式会社 处理由含氟聚合物制造工序产生的水性流体的方法和系统
EP3473336B1 (de) * 2017-10-17 2020-12-02 Sartorius Stedim Biotech GmbH Kieselgurzusammensetzung mit geringem endotoxingehalt
US11453877B2 (en) * 2019-05-15 2022-09-27 Translate Bio, Inc. Methods for purification of messenger RNA

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3639949A1 (de) * 1986-11-22 1988-06-09 Diagen Inst Molekularbio Verfahren zur trennung von langkettigen nukleinsaeuren
US5075430A (en) * 1988-12-12 1991-12-24 Bio-Rad Laboratories, Inc. Process for the purification of DNA on diatomaceous earth
NL8900725A (nl) * 1989-03-23 1990-10-16 Az Univ Amsterdam Werkwijze en combinatie van middelen voor het isoleren van nucleinezuur.
US5234809A (en) * 1989-03-23 1993-08-10 Akzo N.V. Process for isolating nucleic acid
MX9202037A (es) * 1991-05-03 1992-11-01 Becton Dickinson Co Purificacion por filtracion de adn.
US5155018A (en) * 1991-07-10 1992-10-13 Hahnemann University Process and kit for isolating and purifying RNA from biological sources
EP0555798B1 (de) * 1992-02-13 1999-05-06 Becton, Dickinson and Company Celithydrat und Reinigung von DNS
DE59506735D1 (de) * 1994-06-14 1999-10-07 Invitek Gmbh Universelles verfahren zur isolierung und reinigung von nukleinsäuren aus extrem geringen mengen sowie sehr stark verunreinigten unterschiedlichsten ausgangsmaterialien

Also Published As

Publication number Publication date
EP1291421A1 (de) 2003-03-12
DE69626957D1 (de) 2003-04-30
DE69626957T2 (de) 2004-03-04
EP0802975A1 (de) 1997-10-29
EP1291421B1 (de) 2008-12-31
JP3808506B2 (ja) 2006-08-16
JPH11502403A (ja) 1999-03-02
DK0802975T3 (da) 2003-07-14
EP0802975B1 (de) 2003-03-26
US5576196A (en) 1996-11-19
ATE419341T1 (de) 2009-01-15
ATE235547T1 (de) 2003-04-15
ES2196140T3 (es) 2003-12-16
WO1996021729A1 (en) 1996-07-18

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Legal Events

Date Code Title Description
8339 Ceased/non-payment of the annual fee