DE69626957D1 - Verfahren zur reduzierung der rns-konzentration in einer mischung biologischen materials unter verwendung von diatomeen-erde - Google Patents

Verfahren zur reduzierung der rns-konzentration in einer mischung biologischen materials unter verwendung von diatomeen-erde

Info

Publication number
DE69626957D1
DE69626957D1 DE69626957T DE69626957T DE69626957D1 DE 69626957 D1 DE69626957 D1 DE 69626957D1 DE 69626957 T DE69626957 T DE 69626957T DE 69626957 T DE69626957 T DE 69626957T DE 69626957 D1 DE69626957 D1 DE 69626957D1
Authority
DE
Germany
Prior art keywords
mixture
concentration
rna
reducing
diatome
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69626957T
Other languages
English (en)
Other versions
DE69626957T2 (de
Inventor
Nancy Horn
Magda Marquet
Jennifer Meek
Gregg Budahazi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Fresh Tracks Therapeutics Inc
Original Assignee
Vical Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Vical Inc filed Critical Vical Inc
Application granted granted Critical
Publication of DE69626957D1 publication Critical patent/DE69626957D1/de
Publication of DE69626957T2 publication Critical patent/DE69626957T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/08—Reducing the nucleic acid content

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Processing Of Solid Wastes (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Treatment Of Sludge (AREA)
  • Disinfection, Sterilisation Or Deodorisation Of Air (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
DE69626957T 1995-01-13 1996-01-03 Verfahren zur reduzierung der rns-konzentration in einer mischung biologischen materials unter verwendung von diatomeen-erde Expired - Lifetime DE69626957T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US08/372,236 US5576196A (en) 1995-01-13 1995-01-13 Process for reducing RNA concentration in a mixture of biological material using diatomaceous earth
US372236 1995-01-13
PCT/US1996/000247 WO1996021729A1 (en) 1995-01-13 1996-01-03 Process for reducing rna concentration in a mixture of biological material using diatomaceous earth

Publications (2)

Publication Number Publication Date
DE69626957D1 true DE69626957D1 (de) 2003-04-30
DE69626957T2 DE69626957T2 (de) 2004-03-04

Family

ID=23467282

Family Applications (2)

Application Number Title Priority Date Filing Date
DE69637798T Expired - Fee Related DE69637798D1 (de) 1995-01-13 1996-01-03 Verfahren zur Reduzierung der RNA-Konzentration in einer Mischung biologischen Materials unter Verwendung von Diatomeen-Erde
DE69626957T Expired - Lifetime DE69626957T2 (de) 1995-01-13 1996-01-03 Verfahren zur reduzierung der rns-konzentration in einer mischung biologischen materials unter verwendung von diatomeen-erde

Family Applications Before (1)

Application Number Title Priority Date Filing Date
DE69637798T Expired - Fee Related DE69637798D1 (de) 1995-01-13 1996-01-03 Verfahren zur Reduzierung der RNA-Konzentration in einer Mischung biologischen Materials unter Verwendung von Diatomeen-Erde

Country Status (8)

Country Link
US (1) US5576196A (de)
EP (2) EP1291421B1 (de)
JP (1) JP3808506B2 (de)
AT (2) ATE419341T1 (de)
DE (2) DE69637798D1 (de)
DK (1) DK0802975T3 (de)
ES (1) ES2196140T3 (de)
WO (1) WO1996021729A1 (de)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2195112T5 (es) 1996-02-06 2015-10-15 Roche Diagnostics Gmbh Procedimiento para la preparación de ácido nucleico purificado y el uso del mismo
FR2746412B1 (fr) * 1996-03-21 1998-06-12 Rhone Poulenc Rorer Sa Purification d'adn plasmidique de qualite pharmaceutique
US7026468B2 (en) 1996-07-19 2006-04-11 Valentis, Inc. Process and equipment for plasmid purification
US7807822B2 (en) 1996-08-01 2010-10-05 Robert Bridenbaugh Methods for purifying nucleic acids
US6268492B1 (en) 1998-08-10 2001-07-31 Chiron Corporation Methods for purifying non-chromosomal nucleic acid molecules from cells
US6617108B1 (en) 1999-07-12 2003-09-09 Technology Licensing Co. Llc Methods and compositions for biotechnical separations using selective precipitation by compaction agents
US20020012990A1 (en) * 1999-12-22 2002-01-31 Lander Russel Jackson Process for the scaleable purification of plasmid DNA
DE10033991A1 (de) * 2000-07-12 2002-01-24 Qiagen Gmbh Verfahren zur Isolierung von Nukleinsäuren
AU2002359522A1 (en) * 2001-11-28 2003-06-10 Applera Corporation Compositions and methods of selective nucleic acid isolation
CA2498518A1 (en) * 2002-09-13 2004-03-25 Valentis, Inc. Apparatus and method for preparative scale purification of nucleic acids
DE60325773D1 (de) * 2002-12-23 2009-02-26 Vical Inc Reinigungsverfahren für plasmid-dna
US8501402B2 (en) 2003-03-24 2013-08-06 Boehringer Ingelheim Rcv Gmbh & Co Kg Methods and devices for producing biomolecules
JP2004000922A (ja) * 2003-03-26 2004-01-08 Toyobo Co Ltd 核酸またはタンパク質抽出用シリカ粒子組成物
AU2006211216B2 (en) * 2005-01-31 2011-02-03 Merck Sharp & Dohme Llc Purification process for plasmid DNA
EP2088196A1 (de) 2008-02-08 2009-08-12 Boehringer Ingelheim RCV GmbH & Co KG Verfahren und Vorrichtungen zur Herstellung von Biomolekülen
WO2010075116A2 (en) * 2008-12-15 2010-07-01 Life Technologies Corporation Nucleic acid purification apparatus and method
CN102906262A (zh) * 2010-07-07 2013-01-30 戴阿冈有限公司 用于细菌总dna内含物的特异性分离方法和用于该分离目的的试剂盒
GB201614050D0 (en) * 2016-08-17 2016-09-28 Glaxosmithkline Ip Dev Ltd Method for purifying viral vectors
US11266934B2 (en) 2017-06-27 2022-03-08 Daikin Industries. Ltd. Method and system for treating aqueous fluid resulting from fluoropolymer production step
EP3473336B1 (de) * 2017-10-17 2020-12-02 Sartorius Stedim Biotech GmbH Kieselgurzusammensetzung mit geringem endotoxingehalt
EP3969584A1 (de) * 2019-05-15 2022-03-23 Translate Bio, Inc. Verfahren zur reinigung von messenger-rna

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3639949A1 (de) * 1986-11-22 1988-06-09 Diagen Inst Molekularbio Verfahren zur trennung von langkettigen nukleinsaeuren
US5075430A (en) * 1988-12-12 1991-12-24 Bio-Rad Laboratories, Inc. Process for the purification of DNA on diatomaceous earth
NL8900725A (nl) * 1989-03-23 1990-10-16 Az Univ Amsterdam Werkwijze en combinatie van middelen voor het isoleren van nucleinezuur.
US5234809A (en) * 1989-03-23 1993-08-10 Akzo N.V. Process for isolating nucleic acid
MX9202037A (es) * 1991-05-03 1992-11-01 Becton Dickinson Co Purificacion por filtracion de adn.
US5155018A (en) * 1991-07-10 1992-10-13 Hahnemann University Process and kit for isolating and purifying RNA from biological sources
DE69324716T2 (de) * 1992-02-13 1999-09-09 Becton Dickinson And Co. Celithydrat und Reinigung von DNS
WO1995034569A1 (de) * 1994-06-14 1995-12-21 Invitek Gmbh Universelles verfahren zur isolierung und reinigung von nukleinsäuren aus extrem geringen mengen sowie sehr stark verunreinigten unterschiedlichsten ausgangsmaterialien

Also Published As

Publication number Publication date
DE69626957T2 (de) 2004-03-04
EP0802975B1 (de) 2003-03-26
JPH11502403A (ja) 1999-03-02
JP3808506B2 (ja) 2006-08-16
ES2196140T3 (es) 2003-12-16
EP1291421A1 (de) 2003-03-12
DE69637798D1 (de) 2009-02-12
ATE235547T1 (de) 2003-04-15
DK0802975T3 (da) 2003-07-14
US5576196A (en) 1996-11-19
EP0802975A1 (de) 1997-10-29
WO1996021729A1 (en) 1996-07-18
EP1291421B1 (de) 2008-12-31
ATE419341T1 (de) 2009-01-15

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