ES2196140T3 - Procedimientos para reducir la concentracion de arn en una mezcla de material biologico utilizando tierra de diatomeas. - Google Patents

Procedimientos para reducir la concentracion de arn en una mezcla de material biologico utilizando tierra de diatomeas.

Info

Publication number
ES2196140T3
ES2196140T3 ES96903384T ES96903384T ES2196140T3 ES 2196140 T3 ES2196140 T3 ES 2196140T3 ES 96903384 T ES96903384 T ES 96903384T ES 96903384 T ES96903384 T ES 96903384T ES 2196140 T3 ES2196140 T3 ES 2196140T3
Authority
ES
Spain
Prior art keywords
concentration
rna
diatome
mix
biological material
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES96903384T
Other languages
English (en)
Inventor
Nancy Horn
Magda Marquet
Jennifer Meek
Gregg Budahazi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Fresh Tracks Therapeutics Inc
Original Assignee
Vical Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Vical Inc filed Critical Vical Inc
Application granted granted Critical
Publication of ES2196140T3 publication Critical patent/ES2196140T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/08—Reducing the nucleic acid content

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Virology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Processing Of Solid Wastes (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Disinfection, Sterilisation Or Deodorisation Of Air (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Treatment Of Sludge (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

ESTA INVENCION TRATA DE UN PROCEDIMIENTO PARA REDUCIR LA CONCENTRACION DE ARN EN UNA MEZCLA DE UN MATERIAL BIOLOGICO, QUE INCLUYE LAS ETAPAS SIGUIENTES: (A) PROPORCIONAR UNA MEZCLA DE UN MATERIAL BIOLOGICO QUE POSEE UNA PRIMERA CONCENTRACION DE ARN; (B) FILTRAR LA MEZCLA A TRAVES DE UN MATERIAL DE TIERRA DE DIATOMEAS PARA PRODUCIR UN FILTRADO QUE POSEE UNA SEGUNDA CONCENTRACION DE ARN, SIENDO ESTA SEGUNDA CONCENTRACION MENOR QUE LA PRIMERA CONCENTRACION; Y (C) RECOGER EL FILTRADO QUE POSEE UNA CONCENTRACION DE ARN REDUCIDA. EN UN ASPECTO, EL MATERIAL DE TIERRA DE DIATOMEAS SIRVE PARA PURIFICAR UN ADN PLASMIDO RECOMBINANTE DE UN COMPONENTE DE ARN EN UNA MEZCLA DE UN MATERIAL BIOLOGICO, Y EN OTRO ASPECTO SIRVE PARA SEPARAR DIFERENTES FORMAS DE ARN SOLUBLE EN UNA MEZCLA DE MATERIAL BIOLOGICO.
ES96903384T 1995-01-13 1996-01-03 Procedimientos para reducir la concentracion de arn en una mezcla de material biologico utilizando tierra de diatomeas. Expired - Lifetime ES2196140T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/372,236 US5576196A (en) 1995-01-13 1995-01-13 Process for reducing RNA concentration in a mixture of biological material using diatomaceous earth

Publications (1)

Publication Number Publication Date
ES2196140T3 true ES2196140T3 (es) 2003-12-16

Family

ID=23467282

Family Applications (1)

Application Number Title Priority Date Filing Date
ES96903384T Expired - Lifetime ES2196140T3 (es) 1995-01-13 1996-01-03 Procedimientos para reducir la concentracion de arn en una mezcla de material biologico utilizando tierra de diatomeas.

Country Status (8)

Country Link
US (1) US5576196A (es)
EP (2) EP0802975B1 (es)
JP (1) JP3808506B2 (es)
AT (2) ATE419341T1 (es)
DE (2) DE69626957T2 (es)
DK (1) DK0802975T3 (es)
ES (1) ES2196140T3 (es)
WO (1) WO1996021729A1 (es)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2195112T5 (es) 1996-02-06 2015-10-15 Roche Diagnostics Gmbh Procedimiento para la preparación de ácido nucleico purificado y el uso del mismo
FR2746412B1 (fr) * 1996-03-21 1998-06-12 Rhone Poulenc Rorer Sa Purification d'adn plasmidique de qualite pharmaceutique
US7026468B2 (en) 1996-07-19 2006-04-11 Valentis, Inc. Process and equipment for plasmid purification
US7807822B2 (en) 1996-08-01 2010-10-05 Robert Bridenbaugh Methods for purifying nucleic acids
US6268492B1 (en) * 1998-08-10 2001-07-31 Chiron Corporation Methods for purifying non-chromosomal nucleic acid molecules from cells
US6617108B1 (en) 1999-07-12 2003-09-09 Technology Licensing Co. Llc Methods and compositions for biotechnical separations using selective precipitation by compaction agents
US20020012990A1 (en) * 1999-12-22 2002-01-31 Lander Russel Jackson Process for the scaleable purification of plasmid DNA
DE10033991A1 (de) * 2000-07-12 2002-01-24 Qiagen Gmbh Verfahren zur Isolierung von Nukleinsäuren
WO2003046146A2 (en) * 2001-11-28 2003-06-05 Applera Corporation Compositions and methods of selective nucleic acid isolation
US7314746B2 (en) 2002-09-13 2008-01-01 Valentis, Inc. Apparatus and method for preparative scale purification of nucleic acids
DE60325773D1 (de) * 2002-12-23 2009-02-26 Vical Inc Reinigungsverfahren für plasmid-dna
US8501402B2 (en) 2003-03-24 2013-08-06 Boehringer Ingelheim Rcv Gmbh & Co Kg Methods and devices for producing biomolecules
JP2004000922A (ja) * 2003-03-26 2004-01-08 Toyobo Co Ltd 核酸またはタンパク質抽出用シリカ粒子組成物
EP1856262B1 (en) * 2005-01-31 2012-08-15 Merck Sharp & Dohme Corp. Upstream and a downstream purification process for large scale production of plasmid dna
EP2088196A1 (en) 2008-02-08 2009-08-12 Boehringer Ingelheim RCV GmbH & Co KG Methods and devices for producing biomolecules
US20110313143A1 (en) * 2008-12-15 2011-12-22 Life Technologies Corporation Nucleic acid purification apparatus and method
CN102906262A (zh) * 2010-07-07 2013-01-30 戴阿冈有限公司 用于细菌总dna内含物的特异性分离方法和用于该分离目的的试剂盒
GB201614050D0 (en) * 2016-08-17 2016-09-28 Glaxosmithkline Ip Dev Ltd Method for purifying viral vectors
WO2019004298A1 (ja) * 2017-06-27 2019-01-03 ダイキン工業株式会社 含フッ素ポリマー製造工程から生じる水性流体を処理する方法およびシステム
EP3473336B1 (en) * 2017-10-17 2020-12-02 Sartorius Stedim Biotech GmbH Diatomaceous earth composition with a low endotoxin content
US11453877B2 (en) * 2019-05-15 2022-09-27 Translate Bio, Inc. Methods for purification of messenger RNA

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3639949A1 (de) * 1986-11-22 1988-06-09 Diagen Inst Molekularbio Verfahren zur trennung von langkettigen nukleinsaeuren
US5075430A (en) * 1988-12-12 1991-12-24 Bio-Rad Laboratories, Inc. Process for the purification of DNA on diatomaceous earth
NL8900725A (nl) * 1989-03-23 1990-10-16 Az Univ Amsterdam Werkwijze en combinatie van middelen voor het isoleren van nucleinezuur.
US5234809A (en) * 1989-03-23 1993-08-10 Akzo N.V. Process for isolating nucleic acid
CA2067712A1 (en) * 1991-05-03 1992-11-04 Daniel L. Woodard Filtration purification of dna
US5155018A (en) * 1991-07-10 1992-10-13 Hahnemann University Process and kit for isolating and purifying RNA from biological sources
EP0555798B1 (en) * 1992-02-13 1999-05-06 Becton, Dickinson and Company Hydrated celite and purification of DNA
JP3761573B2 (ja) * 1994-06-14 2006-03-29 インヴィテーク ゲーエムベーハー 極度に少量でかつ非常に強く汚染された非常に種々の出発物質から核酸を単離および精製する一般的方法

Also Published As

Publication number Publication date
JP3808506B2 (ja) 2006-08-16
DK0802975T3 (da) 2003-07-14
JPH11502403A (ja) 1999-03-02
ATE235547T1 (de) 2003-04-15
EP0802975B1 (en) 2003-03-26
EP1291421B1 (en) 2008-12-31
WO1996021729A1 (en) 1996-07-18
DE69626957T2 (de) 2004-03-04
DE69637798D1 (de) 2009-02-12
EP1291421A1 (en) 2003-03-12
ATE419341T1 (de) 2009-01-15
EP0802975A1 (en) 1997-10-29
US5576196A (en) 1996-11-19
DE69626957D1 (de) 2003-04-30

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