DE69634696D1 - Verfahren und testsystem zur hybridisierungsanalyse unter verwendung von peptidnukleinsäure-sonden - Google Patents

Verfahren und testsystem zur hybridisierungsanalyse unter verwendung von peptidnukleinsäure-sonden

Info

Publication number
DE69634696D1
DE69634696D1 DE69634696T DE69634696T DE69634696D1 DE 69634696 D1 DE69634696 D1 DE 69634696D1 DE 69634696 T DE69634696 T DE 69634696T DE 69634696 T DE69634696 T DE 69634696T DE 69634696 D1 DE69634696 D1 DE 69634696D1
Authority
DE
Germany
Prior art keywords
nucleic acid
test system
peptide nucleic
hybridization analysis
acid problems
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69634696T
Other languages
English (en)
Other versions
DE69634696T2 (de
Inventor
Martin Fuchs
Michael Egholm
Heather O'keefe
Xian W Yao
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Applied Biosystems LLC
Original Assignee
PerSeptive Biosystems Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by PerSeptive Biosystems Inc filed Critical PerSeptive Biosystems Inc
Application granted granted Critical
Publication of DE69634696D1 publication Critical patent/DE69634696D1/de
Publication of DE69634696T2 publication Critical patent/DE69634696T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
    • G01N27/44726—Arrangements for investigating the separated zones, e.g. localising zones by optical means using specific dyes, markers or binding molecules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Pathology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Physics & Mathematics (AREA)
  • Electrochemistry (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DE69634696T 1995-10-06 1996-10-04 Verfahren und testsystem zur hybridisierungsanalyse unter verwendung von peptidnukleinsäure-sonden Expired - Lifetime DE69634696T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US495395P 1995-10-06 1995-10-06
US4953 1995-10-06
PCT/US1996/015918 WO1997012995A1 (en) 1995-10-06 1996-10-04 Methods and kit for hybridization analysis using peptide nucleic acid probes

Publications (2)

Publication Number Publication Date
DE69634696D1 true DE69634696D1 (de) 2005-06-09
DE69634696T2 DE69634696T2 (de) 2006-01-19

Family

ID=21713369

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69634696T Expired - Lifetime DE69634696T2 (de) 1995-10-06 1996-10-04 Verfahren und testsystem zur hybridisierungsanalyse unter verwendung von peptidnukleinsäure-sonden

Country Status (5)

Country Link
US (2) US20020012902A1 (de)
EP (1) EP0854937B9 (de)
JP (1) JPH11512617A (de)
DE (1) DE69634696T2 (de)
WO (1) WO1997012995A1 (de)

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AU3829197A (en) * 1996-09-03 1998-03-26 Pioneer Hi-Bred International, Inc. Method and apparatus for single molecule two color fluorescent detection and molecular weight and concentration determination
US6251591B1 (en) * 1997-02-27 2001-06-26 Lorne Park Research, Inc. Quantitative method for detecting nucleotide concentration
US5846729A (en) * 1997-02-27 1998-12-08 Lorne Park Research, Inc. Assaying nucleotides in solution using a fluorescent intensity quenching effect
US6046004A (en) * 1997-02-27 2000-04-04 Lorne Park Research, Inc. Solution hybridization of nucleic acids with antisense probes having modified backbones
DK0975800T3 (da) * 1997-02-27 2003-09-22 Ingeneus Corp Analyse af nukleotider i opløsning under anvendelse af PNA-prober
US6060242A (en) * 1997-02-27 2000-05-09 Lorne Park Research, Inc. PNA diagnostic methods
AU731909B2 (en) 1997-07-01 2001-04-05 Isis Pharmaceuticals, Inc. Compositions and methods for the delivery of oligonucleotides via the alimentary canal
US20030165953A1 (en) * 1997-07-09 2003-09-04 Isao Karube Method for detecting DNA with probe PNA
US6255050B1 (en) 1998-05-22 2001-07-03 Lorne Park Research, Inc. Dynamic hybridization system
WO2000034521A1 (en) * 1998-12-08 2000-06-15 Boston Probes, Inc. Methods, kits and compositions for the identification of nucleic acids electrostatically bound to matrices
DE69938400T2 (de) * 1998-12-08 2009-04-23 Boston Probes, Inc., Bedford Verfahren, Kits und Zusammensetzungen zur Identifizierung von elektrostatisch an Matrizen gebundenen Nukleinsäuren
US6361671B1 (en) * 1999-01-11 2002-03-26 The Regents Of The University Of California Microfabricated capillary electrophoresis chip and method for simultaneously detecting multiple redox labels
WO2000057170A1 (en) * 1999-03-19 2000-09-28 The Governors Of The University Of Alberta Automated 2-dimensional analysis of biological and other samples
US6645733B1 (en) 1999-06-25 2003-11-11 Ingeneus Corporation Fluorescent intensity method for assaying binding between proteins or peptides
US6312956B1 (en) 1999-10-01 2001-11-06 Vanderbilt University Nuclear targeted peptide nucleic acid oligomer
US7052844B2 (en) * 1999-12-21 2006-05-30 Ingeneus, Inc. Purification of DS-DNA using heteropolymeric capture probes and a triplex, quadruplex or homologous duplex binding mechanism
JP2004513625A (ja) * 2000-08-30 2004-05-13 エイブイアイ　バイオファーマ，　インコーポレイテッド オリゴヌクレオチドアナログの分析のための方法
EP1320745A4 (de) * 2000-09-01 2008-10-01 Applera Corp System und verfahren zur temperaturgradienten-kapillarelektrophorese
CA2425413A1 (en) * 2000-10-11 2002-04-18 Spectrumedix Corporation System and method for determining the presence of methylated cytosines in polynucleotides
JP2005524384A (ja) 2001-09-24 2005-08-18 ボストン　プローブス，インコーポレイテッド ゲノム核酸におけるランダム分布反復配列への検出可能なプローブ結合の抑制に関する方法、キットおよび組成物
WO2003033743A1 (en) * 2001-10-18 2003-04-24 Spectrumedix Corporation System and method for temperature gradient capillary electrophoresis
EP1451674A4 (de) * 2001-10-26 2008-08-20 Sequenom Inc Verfahren und vorrichtung zur parallelen ausgabe definierter volumen von festpartikeln
GB0205455D0 (en) 2002-03-07 2002-04-24 Molecular Sensing Plc Nucleic acid probes, their synthesis and use
AU2003230717A1 (en) 2002-03-21 2003-10-08 Boston Probes, Inc. Pna oligomers, oligomers sets, methods and kits pertaining to the detection of bacillus anthracis
US6872530B2 (en) * 2002-04-24 2005-03-29 Spectrumedix, Llc Method for determining the presence of DNA variants using peptide nucleic acid probes
EP1511836A1 (de) * 2002-06-07 2005-03-09 Millipore Corporation Kit und verfahren zur mikrobiologischen untersuchung einer flüssigen probe vor ort
US20040072217A1 (en) * 2002-06-17 2004-04-15 Affymetrix, Inc. Methods of analysis of linkage disequilibrium
US20040178070A1 (en) * 2002-07-16 2004-09-16 Zhaowei Liu Method and system for comparative genomics for organisms using temperature gradient electrophoresis
US7049073B2 (en) 2002-10-30 2006-05-23 The University Of Chicago Double stranded nucleic acid biochips
JP4361057B2 (ja) * 2003-04-30 2009-11-11 モイル，ウィリアム，アール 生体分子検出および細胞分類用センサ
US7303879B2 (en) * 2003-07-31 2007-12-04 Applera Corporation Determination of SNP allelic frequencies using temperature gradient electrophoresis
US7897747B2 (en) * 2006-05-25 2011-03-01 The Board Of Trustees Of The Leland Stanford Junior University Method to produce single stranded DNA of defined length and sequence and DNA probes produced thereby
EP1995328A1 (de) * 2007-05-22 2008-11-26 Koninklijke Philips Electronics N.V. Verfahren zur Erkennung einer Sonden-Target-Bindung
US20090215050A1 (en) * 2008-02-22 2009-08-27 Robert Delmar Jenison Systems and methods for point-of-care amplification and detection of polynucleotides
JP5395076B2 (ja) * 2008-07-22 2014-01-22 アークレイ株式会社 キャピラリー電気泳動法による分析装置および分析方法
WO2010010858A1 (ja) 2008-07-22 2010-01-28 アークレイ株式会社 キャピラリー電気泳動法による分析装置
WO2010141131A1 (en) 2009-06-04 2010-12-09 Lockheed Martin Corporation Multiple-sample microfluidic chip for dna analysis
GB2497501A (en) 2010-10-15 2013-06-12 Lockheed Corp Micro fluidic optic design
US9322054B2 (en) 2012-02-22 2016-04-26 Lockheed Martin Corporation Microfluidic cartridge
US10349589B2 (en) 2016-09-08 2019-07-16 Hemex Health, Inc. Diagnostics systems and methods
EP3510396B1 (de) 2016-09-08 2021-12-08 Hemex Health, Inc. Diagnosesystem
US10538808B2 (en) 2017-05-26 2020-01-21 Vibrant Holdings, Llc Photoactive compounds and methods for biomolecule detection and sequencing
WO2019060633A2 (en) * 2017-09-22 2019-03-28 Prozyme, Inc. USE OF POLYAMINES AS RESOLUTION AGENTS FOR CAPILLARY ELECTROPHORESIS OF GLYCANES MARKETED USING GELS
US11740203B2 (en) 2019-06-25 2023-08-29 Hemex Health, Inc. Diagnostics systems and methods
CN112326762B (zh) * 2020-10-29 2023-03-21 常州大学 一种毛细管电泳检测dna四面体的方法

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DK51092D0 (da) * 1991-05-24 1992-04-15 Ole Buchardt Oligonucleotid-analoge betegnet pna, monomere synthoner og fremgangsmaade til fremstilling deraf samt anvendelser deraf
US6045995A (en) * 1994-08-24 2000-04-04 Isis Pharmaceuticals, Inc. Capillary electrophoretic detection of nucleic acids
US5582705A (en) * 1995-05-19 1996-12-10 Iowa State University Research Foundation, Inc. Multiplexed capillary electrophoresis system

Also Published As

Publication number Publication date
DE69634696T2 (de) 2006-01-19
EP0854937A1 (de) 1998-07-29
EP0854937B1 (de) 2005-05-04
EP0854937B9 (de) 2006-06-28
JPH11512617A (ja) 1999-11-02
WO1997012995A1 (en) 1997-04-10
US20020012902A1 (en) 2002-01-31
US20050053944A1 (en) 2005-03-10

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Owner name: APPLIED BIOSYSTEMS, LLC (N. D. GES. D. STAATES, US