DE69612457T2 - Screening-Verfahren zur Ligand-Identifizierung von Zielproteinen - Google Patents

Screening-Verfahren zur Ligand-Identifizierung von Zielproteinen

Info

Publication number
DE69612457T2
DE69612457T2 DE69612457T DE69612457T DE69612457T2 DE 69612457 T2 DE69612457 T2 DE 69612457T2 DE 69612457 T DE69612457 T DE 69612457T DE 69612457 T DE69612457 T DE 69612457T DE 69612457 T2 DE69612457 T2 DE 69612457T2
Authority
DE
Germany
Prior art keywords
target proteins
target protein
screening method
ligands
ligand identification
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69612457T
Other languages
English (en)
Other versions
DE69612457D1 (de
Inventor
Andrew Pakula
James Bowie
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Anadys Pharmaceuticals Inc
Original Assignee
Anadys Pharmaceuticals Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Anadys Pharmaceuticals Inc filed Critical Anadys Pharmaceuticals Inc
Publication of DE69612457D1 publication Critical patent/DE69612457D1/de
Application granted granted Critical
Publication of DE69612457T2 publication Critical patent/DE69612457T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Organic Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Zoology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
DE69612457T 1995-10-25 1996-10-17 Screening-Verfahren zur Ligand-Identifizierung von Zielproteinen Expired - Fee Related DE69612457T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US54788995A 1995-10-25 1995-10-25

Publications (2)

Publication Number Publication Date
DE69612457D1 DE69612457D1 (de) 2001-05-17
DE69612457T2 true DE69612457T2 (de) 2001-11-08

Family

ID=24186560

Family Applications (2)

Application Number Title Priority Date Filing Date
DE69612457T Expired - Fee Related DE69612457T2 (de) 1995-10-25 1996-10-17 Screening-Verfahren zur Ligand-Identifizierung von Zielproteinen
DE0770876T Pending DE770876T1 (de) 1995-10-25 1996-10-17 Screening-Verfahren zur Ligand-Identifizierung von Zielproteinen

Family Applications After (1)

Application Number Title Priority Date Filing Date
DE0770876T Pending DE770876T1 (de) 1995-10-25 1996-10-17 Screening-Verfahren zur Ligand-Identifizierung von Zielproteinen

Country Status (14)

Country Link
EP (1) EP0770876B1 (de)
JP (1) JP2952848B2 (de)
KR (1) KR0161256B1 (de)
AT (1) ATE200579T1 (de)
AU (1) AU698862B2 (de)
BR (1) BR9604352A (de)
CA (1) CA2184195C (de)
DE (2) DE69612457T2 (de)
DK (1) DK0770876T3 (de)
ES (1) ES2158269T3 (de)
GR (1) GR3036138T3 (de)
IL (1) IL119149A (de)
MX (1) MX9604183A (de)
PT (1) PT770876E (de)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2253587C (en) 1996-05-09 2008-01-29 3-Dimensional Pharmaceuticals, Inc. Microplate thermal shift assay and apparatus for ligand development and multi-variable protein chemistry optimization
US6453246B1 (en) 1996-11-04 2002-09-17 3-Dimensional Pharmaceuticals, Inc. System, method, and computer program product for representing proximity data in a multi-dimensional space
US6571227B1 (en) 1996-11-04 2003-05-27 3-Dimensional Pharmaceuticals, Inc. Method, system and computer program product for non-linear mapping of multi-dimensional data
EP0935784A2 (de) 1996-11-04 1999-08-18 3-Dimensional Pharmaceuticals, Inc. System, verfahren und rechnerprogramm zur identifizierung von chemischen zusammensetzungen mit gewünschten eigenschaften
NZ504483A (en) 1997-11-12 2002-11-26 Dimensional Pharm Inc High throughput method for functionally classifying proteins by contacting the protein with different molecules and determining the effect on the proteins stability
US6569631B1 (en) 1998-11-12 2003-05-27 3-Dimensional Pharmaceuticals, Inc. Microplate thermal shift assay for ligand development using 5-(4″dimethylaminophenyl)-2-(4′-phenyl)oxazole derivative fluorescent dyes
GB9904395D0 (en) * 1999-02-25 1999-04-21 Fluorescience Ltd Protein assay
US6972198B2 (en) * 1999-02-26 2005-12-06 Cyclacel, Ltd. Methods and compositions using protein binding partners
JP4493125B2 (ja) * 1999-05-07 2010-06-30 独立行政法人理化学研究所 相互作用するタンパク質の検出方法
US6582907B1 (en) 1999-12-09 2003-06-24 Pharmacia & Upjohn Company Use of fluorescence correlation spectroscopy to identify compounds that bind to target species under isothermal denaturing conditions
US6376180B1 (en) * 1999-12-09 2002-04-23 Pharmacia & Upjohn Company Methods of identifying compounds that bind to target species under isothermal denaturing conditions
US7416524B1 (en) 2000-02-18 2008-08-26 Johnson & Johnson Pharmaceutical Research & Development, L.L.C. System, method and computer program product for fast and efficient searching of large chemical libraries
WO2001065462A2 (en) 2000-02-29 2001-09-07 3-Dimensional Pharmaceuticals, Inc. Method and computer program product for designing combinatorial arrays
US7039621B2 (en) 2000-03-22 2006-05-02 Johnson & Johnson Pharmaceutical Research & Development, L.L.C. System, method, and computer program product for representing object relationships in a multidimensional space
WO2001075790A2 (en) 2000-04-03 2001-10-11 3-Dimensional Pharmaceuticals, Inc. Method, system, and computer program product for representing object relationships in a multidimensional space
KR20010112840A (ko) * 2000-06-15 2001-12-22 김유삼 기능성 단백질의 패턴분석을 이용한 진단 시스템
WO2002061419A1 (en) 2001-01-29 2002-08-08 3-Dimensional Pharmaceuticals, Inc. Method, system, and computer program product for analyzing combinatorial libraries
EP1433859B1 (de) * 2001-09-03 2006-11-29 Kyowa Hakko Kogyo Co., Ltd. Verfahren zur bestimmung eines zielproteins eines arzneistoffs
US7063946B2 (en) 2001-09-10 2006-06-20 Meso Scale Technologies, Llc. Methods, reagents, kits and apparatus for protein function analysis
CA2601869A1 (en) 2005-08-12 2007-02-22 Astellas Pharma Inc. Method for identifying target protein of agent and method for screening therapeutic agent for diabetes using target protein
DE102006027259A1 (de) * 2006-06-09 2007-12-13 Protagen Ag Protein-Biochip zum differentiellen Screenen von Protein-Protein-Wechselwirkungen
WO2018152329A1 (en) 2017-02-15 2018-08-23 Ohio State Innovation Foundation Heteroaromatic electrophiles and methods of using thereof
CN114076826A (zh) * 2020-08-20 2022-02-22 中国科学院大连化学物理研究所 一种微球辅助的基于蛋白加热沉淀的药物靶蛋白筛选方法
GB202118173D0 (en) * 2021-12-15 2022-01-26 Medicines Discovery Catpult Ltd Protein stability assay

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5231755B2 (de) * 1973-01-08 1977-08-17
US4859609A (en) * 1986-04-30 1989-08-22 Genentech, Inc. Novel receptors for efficient determination of ligands and their antagonists or agonists
JPH06503948A (ja) * 1990-08-17 1994-05-12 ボストン・ユニヴァーシティー アミロイドβ―蛋白質のプレカーサーの異常分解をひき起こすプロテアーゼ
US5506097A (en) * 1990-08-24 1996-04-09 President And Fellows Of Harvard College Method for inhibiting β-protein enzymatic activity
WO1993014781A1 (en) * 1992-01-24 1993-08-05 The Regents Of The University Of California Novel peptides and method for altering the activity of allosteric proteins
CA2115900A1 (en) * 1993-02-22 1994-08-23 Gerald W. Becker Pharmaceutical screens and antibodies
US5585277A (en) * 1993-06-21 1996-12-17 Scriptgen Pharmaceuticals, Inc. Screening method for identifying ligands for target proteins
JP2000502440A (ja) 1995-12-07 2000-02-29 スクリプトジェン・ファーマスーティカルズ，インコーポレイテッド リガンドを識別するための蛍光に基づくスクリーニング方法
WO1997020925A1 (en) * 1995-12-08 1997-06-12 Hybridon, Inc. Modified vegf antisense oligonucleotides for treatment of skin disorders
CA2253587C (en) * 1996-05-09 2008-01-29 3-Dimensional Pharmaceuticals, Inc. Microplate thermal shift assay and apparatus for ligand development and multi-variable protein chemistry optimization
US8082993B2 (en) 2009-03-12 2011-12-27 Halliburton Energy Services, Inc. One trip gravel pack assembly

Also Published As

Publication number Publication date
DE69612457D1 (de) 2001-05-17
PT770876E (pt) 2001-09-28
DE770876T1 (de) 1999-07-22
EP0770876A1 (de) 1997-05-02
CA2184195C (en) 2002-04-16
KR970022315A (ko) 1997-05-28
CA2184195A1 (en) 1997-04-26
AU698862B2 (en) 1998-11-12
KR0161256B1 (ko) 1999-03-30
GR3036138T3 (en) 2001-09-28
JPH09178746A (ja) 1997-07-11
AU6429896A (en) 1997-05-01
IL119149A (en) 2002-03-10
IL119149A0 (en) 1996-11-14
ES2158269T3 (es) 2001-09-01
BR9604352A (pt) 1998-06-16
DK0770876T3 (da) 2001-08-06
ATE200579T1 (de) 2001-04-15
JP2952848B2 (ja) 1999-09-27
EP0770876B1 (de) 2001-04-11
MX9604183A (es) 1998-04-30

Similar Documents

Publication Publication Date Title
DE69612457D1 (de) Screening-Verfahren zur Ligand-Identifizierung von Zielproteinen
DE68907407T2 (de) Verfahren zur sequenzanalyse von dna.
DE69126664T2 (de) Bestimmung von analyten in biologischen flüssigkeiten in gegenwart von störenden substanzen
DE3887168D1 (de) Verfahren zur quantitativen analyse von mineralien.
DE69122494T2 (de) Vorrichtung zur transdermalen Applikation von Protein- oder Peptid-Medikamenten
DE68909962T2 (de) Verfahren und Vorrichtung zur quantitativen Bestimmung von Trihalomethanen.
DE59007613D1 (de) Verfahren zum Nachweis und zur Bestimmung von Humanserumalbumin in biologischen Flüssigkeiten mit Hilfe fluorogener Cyanin-Farbstoffe.
DE3678489D1 (de) Verfahren zur quantitativen bestimmung von protein c und aktivatorpraeparat zur durchfuehrung des verfahrens.
DE3863285D1 (de) Verfahren und reagenz zur bestimmung von persaeuren.
ATA43187A (de) Verfahren zur quantitativen bestimmung von nucleinsaeure-molekuelen und reagenziensatz hierfuer
DE69312185D1 (de) Chromatographischer Sandwichtest zur Bestimmung von spezifischen Antikörpern und Vorrichtung hierfür
DE3889279D1 (de) Diagnostisches Mittel und Verfahren zur Bestimmung von Apolipoprotein B.
DE68909995T2 (de) Reagenz zur Bestimmung von Kreatinkinase und Messverfahren dafür.
DE69016858T2 (de) Verfahren und Reagenziensatz zur Bestimmung der funktionalen Aktivität von Protein S in menschlichem Plasma.
DK0445626T3 (da) Funktionel test til bestemmelse af protein S-aktivitet.
DE69007155T2 (de) Automatisierte Schnittentwicklungsanalyse von mehrfachen Proben.
DE59008687D1 (de) Verfahren zur selektiven komplexometrischen quantitativen Bestimmung von N-(Phosphonomethyl)-glycin-(glifosat(R)) und Derivat davon.
ATE182215T1 (de) Verfahren zur bestimmung von rheumafaktoren und mittel zur durchführung des verfahrens
DE3783840T2 (de) Verfahren zur bestimmung von plasma-protein und testsatz dafuer.
DE58907208D1 (de) Verfahren zur Bestimmung von Antikörpern.
DE3778375D1 (de) Reagenzsatz, seine verwendung und diagnostisches verfahren zur bestimmung von humanen pankreasspezifischen proteinen.
ATE91156T1 (de) Verfahren zur sequenzanalyse von dna.
DE3775649D1 (de) Verfahren zur quantitativen bestimmung der vitamin-b12 und reagenz zur bestimmung von vitamin-b12.
ATE100198T1 (de) Verfahren zur quantitativen analyse von mineralien.
DE406296T1 (de) Verfahren zur sequenzanalyse von dna.

Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee