DE69534079D1 - Deblockierung des gemeinsamen syntheseweges von aromatischen aminosäuren - Google Patents

Deblockierung des gemeinsamen syntheseweges von aromatischen aminosäuren

Info

Publication number
DE69534079D1
DE69534079D1 DE69534079T DE69534079T DE69534079D1 DE 69534079 D1 DE69534079 D1 DE 69534079D1 DE 69534079 T DE69534079 T DE 69534079T DE 69534079 T DE69534079 T DE 69534079T DE 69534079 D1 DE69534079 D1 DE 69534079D1
Authority
DE
Germany
Prior art keywords
synthase
dna sequences
sequences encoding
deblocking
amino acids
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69534079T
Other languages
English (en)
Other versions
DE69534079T2 (de
Inventor
John W Frost
Kristi D Snell
Karen M Frost
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Purdue Research Foundation
Original Assignee
Purdue Research Foundation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Purdue Research Foundation filed Critical Purdue Research Foundation
Application granted granted Critical
Publication of DE69534079D1 publication Critical patent/DE69534079D1/de
Publication of DE69534079T2 publication Critical patent/DE69534079T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
    • C12N9/1092—3-Phosphoshikimate 1-carboxyvinyltransferase (2.5.1.19), i.e. 5-enolpyruvylshikimate-3-phosphate synthase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/22—Tryptophan; Tyrosine; Phenylalanine; 3,4-Dihydroxyphenylalanine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/22—Tryptophan; Tyrosine; Phenylalanine; 3,4-Dihydroxyphenylalanine
    • C12P13/222—Phenylalanine

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Nitrogen Condensed Heterocyclic Rings (AREA)
  • Hydrogenated Pyridines (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
DE69534079T 1994-06-09 1995-06-08 Deblockierung des gemeinsamen syntheseweges von aromatischen aminosäuren Expired - Lifetime DE69534079T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US08/257,354 US5776736A (en) 1992-12-21 1994-06-09 Deblocking the common pathway of aromatic amino acid synthesis
PCT/US1995/007321 WO1995033843A1 (en) 1994-06-09 1995-06-08 Deblocking the common pathway of aromatic amino acid synthesis
US257354 1999-02-25

Publications (2)

Publication Number Publication Date
DE69534079D1 true DE69534079D1 (de) 2005-04-21
DE69534079T2 DE69534079T2 (de) 2006-04-13

Family

ID=22975972

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69534079T Expired - Lifetime DE69534079T2 (de) 1994-06-09 1995-06-08 Deblockierung des gemeinsamen syntheseweges von aromatischen aminosäuren

Country Status (6)

Country Link
US (1) US5776736A (de)
EP (1) EP0763127B1 (de)
AT (1) ATE291090T1 (de)
DE (1) DE69534079T2 (de)
ES (1) ES2240973T3 (de)
WO (1) WO1995033843A1 (de)

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DE69637492T2 (de) 1995-05-05 2009-06-04 Genencor International, Inc., Palo Alto Anwendung von Glukosetransportmutanten zur Herstellung von Verbindungen des aromatischen Syntheseweges
EP0854188A3 (de) * 1997-01-21 2000-02-09 Smithkline Beecham Streptococcus pneumoniae aroE Polypeptiden und Polynuleotiden
EP0861895A3 (de) * 1997-02-21 2000-01-26 Smithkline Beecham Corporation AroE Shikimate 5 Dehydrogenase aus Staphylococcus Aureus
US6210937B1 (en) 1997-04-22 2001-04-03 Bechtel Bwxt Idaho, Llc Development of genetically engineered bacteria for production of selected aromatic compounds
US6911331B2 (en) 1998-07-21 2005-06-28 E. I. Du Pont De Nemours And Company Chorismate biosynthesis enzymes
US6653531B1 (en) 1998-07-21 2003-11-25 E. I. Du Pont De Nemours And Company Chorismate synthase from plants
EP1098984A2 (de) * 1998-07-21 2001-05-16 E.I. Du Pont De Nemours And Company Chorismatbiosynthese enzyme
AU6043399A (en) * 1998-09-18 2000-04-10 Board Of Trustees Of Michigan State University Synthesis of vanillin from a carbon source
US6030819A (en) * 1998-09-28 2000-02-29 General Electric Company Genetically engineered microorganisms and method for producing 4-hydroxybenzoic acid
AU5299000A (en) * 1999-05-28 2000-12-18 Tony Romeo A global regulator used to enhance the synthesis of aromatic substances
US6472190B1 (en) * 2000-03-16 2002-10-29 Board Of Trustees Operating Michigan State Univerisity Biocatalytic synthesis of galloid organics
US20030109014A1 (en) * 2000-03-17 2003-06-12 Kevin Burke Process for the fermentative preparation of L-amino acids with amplification of the tkt gene
AU5982200A (en) * 2000-03-17 2001-09-24 Degussa A.G. Process for the fermentative preparation of l-amino acids with amplification of the tkt gene
US6465217B1 (en) * 2000-07-05 2002-10-15 Paradigm Genetics, Inc. Methods and compositions for the modulation of chorismate synthase and chorismate mutase expression or activity in plants
BR0313306A (pt) * 2002-08-06 2005-06-14 Dsm Ip Assets Bv Processo para o preparo de l-3,4-dihidroxifenilalanina através de fermentação aeróbica de um microorganismo
US20050079617A1 (en) 2003-10-03 2005-04-14 Cervin Marguerite A. Glucose transport mutants for production of biomaterial
WO2004033421A2 (en) * 2002-10-04 2004-04-22 Genencor International, Inc. Improved production of bacterial strains
US7056742B2 (en) * 2003-06-16 2006-06-06 E. I. Du Pont De Nemours And Company High Level production of arbutin in green plants
EP1899453B1 (de) 2005-06-07 2013-12-18 Ocean Nutrition Canada Limited Eukaryotische mikroorganismen zur herstellung von lipiden und antioxidantien
JP2007117082A (ja) * 2005-09-27 2007-05-17 Ajinomoto Co Inc Ｌ−アミノ酸生産菌及びｌ−アミノ酸の製造法
JP2009165355A (ja) 2006-04-28 2009-07-30 Ajinomoto Co Inc Ｌ−アミノ酸を生産する微生物及びｌ−アミノ酸の製造法
US9023616B2 (en) * 2006-08-01 2015-05-05 Dsm Nutritional Products Ag Oil producing microbes and method of modification thereof
RU2006129690A (ru) 2006-08-16 2008-02-27 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ) (RU) СПОСОБ ПОЛУЧЕНИЯ L-АМИНОКИСЛОТЫ С ИСПОЛЬЗОВАНИЕМ БАКТЕРИЙ СЕМЕЙСТВА Enterobacteriaceae, В КОТОРОЙ ОСЛАБЛЕНА ЭКСПРЕССИЯ ГЕНА ydiN, ГЕНА ydiB ИЛИ ИХ КОМБИНАЦИИ
JP2010017081A (ja) * 2006-10-10 2010-01-28 Ajinomoto Co Inc Ｌ−アミノ酸の製造法
JP2010017082A (ja) * 2006-10-10 2010-01-28 Ajinomoto Co Inc Ｌ−アミノ酸の製造法
JP2010041920A (ja) 2006-12-19 2010-02-25 Ajinomoto Co Inc Ｌ−アミノ酸の製造法
CN101627110B (zh) * 2007-01-22 2014-08-13 味之素株式会社 生产l-氨基酸的微生物和l-氨基酸的生产方法
JP2010088301A (ja) 2007-02-01 2010-04-22 Ajinomoto Co Inc Ｌ−アミノ酸の製造法
JP2010110216A (ja) 2007-02-20 2010-05-20 Ajinomoto Co Inc Ｌ−アミノ酸または核酸の製造方法
EP2145010A1 (de) * 2007-04-13 2010-01-20 Monsanto Technology, LLC Verwendung von glyphosat zur produktion von shikimsäure in mikroorganismen
WO2009052303A2 (en) * 2007-10-16 2009-04-23 Monsanto Technology Llc Processes for producing and recovering shikimic acid
JP2011067095A (ja) 2008-01-10 2011-04-07 Ajinomoto Co Inc 発酵法による目的物質の製造法
EP2343371B1 (de) 2008-09-05 2015-10-21 Ajinomoto Co., Inc. Verfahren zur produktion von l-aminosäure
EP2336347B1 (de) * 2008-09-08 2017-03-15 Ajinomoto Co., Inc. L-aminosäure-produzierender mikroorganismus und verfahren zur herstellung von l-aminosäure
JP2012029565A (ja) 2008-11-27 2012-02-16 Ajinomoto Co Inc Ｌ−アミノ酸の製造法
JP2012223091A (ja) 2009-08-25 2012-11-15 Ajinomoto Co Inc Ｌ−アミノ酸の製造法
JP2012223092A (ja) 2009-08-28 2012-11-15 Ajinomoto Co Inc Ｌ−アミノ酸の製造法
JP2013013329A (ja) 2009-11-06 2013-01-24 Ajinomoto Co Inc Ｌ−アミノ酸の製造法
EP2591112B1 (de) 2010-07-06 2017-08-23 Yeda Research and Development Co. Ltd. Transgene pflanzen mit geänderter dahp-synthase-aktivität
CN103930557A (zh) 2011-11-11 2014-07-16 味之素株式会社 利用发酵法制造目标物质的方法
PT2970926T (pt) 2013-03-13 2018-03-22 Dsm Nutritional Products Ag Modificação genética de microrganismos
PE20150681A1 (es) 2013-05-13 2015-05-15 Ajinomoto Kk Metodo para producir l-aminoacidos
JP5958653B2 (ja) 2013-10-02 2016-08-02 味の素株式会社 アンモニア制御装置およびアンモニア制御方法
JP6459962B2 (ja) 2013-10-21 2019-01-30 味の素株式会社 Ｌ−アミノ酸の製造法
JP6519476B2 (ja) 2013-10-23 2019-05-29 味の素株式会社 目的物質の製造法
JP2017131111A (ja) 2014-06-03 2017-08-03 味の素株式会社 Ｌ−アミノ酸の製造法
JP7066977B2 (ja) 2017-04-03 2022-05-16 味の素株式会社 Ｌ－アミノ酸の製造法
WO2020071538A1 (en) 2018-10-05 2020-04-09 Ajinomoto Co., Inc. Method for producing target substance by bacterial fermentation

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JPS57170184A (en) * 1980-07-18 1982-10-20 Unisearch Ltd Mutant of e. coli and production of phenylalanine
JPH0732710B2 (ja) * 1983-05-28 1995-04-12 協和醗酵工業株式会社 フエニ−ルアラニンの製造法
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JPS61247392A (ja) * 1985-02-04 1986-11-04 エンジニツクス　インコ−ポレ−テツド ２種またはより多くのアミノ酸を同時に産生できる組換え微生物
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Also Published As

Publication number Publication date
DE69534079T2 (de) 2006-04-13
EP0763127A1 (de) 1997-03-19
WO1995033843A1 (en) 1995-12-14
ES2240973T3 (es) 2005-10-16
EP0763127A4 (de) 1998-04-01
EP0763127B1 (de) 2005-03-16
ATE291090T1 (de) 2005-04-15
US5776736A (en) 1998-07-07

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