DE69214571D1 - Verfahren zur Analyse von einem Protein oder einem Peptid - Google Patents

Verfahren zur Analyse von einem Protein oder einem Peptid

Info

Publication number
DE69214571D1
DE69214571D1 DE69214571T DE69214571T DE69214571D1 DE 69214571 D1 DE69214571 D1 DE 69214571D1 DE 69214571 T DE69214571 T DE 69214571T DE 69214571 T DE69214571 T DE 69214571T DE 69214571 D1 DE69214571 D1 DE 69214571D1
Authority
DE
Germany
Prior art keywords
peptide
protein
analysis
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69214571T
Other languages
English (en)
Other versions
DE69214571T2 (de
Inventor
Tuyoaki Uchida
Akira Tsugita
Keiji C O Pana-Height Takamoto
Kazuo Satake
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Seiko Instruments Inc
Original Assignee
Seiko Instruments Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from JP3217437A external-priority patent/JP2686505B2/ja
Priority claimed from JP3300818A external-priority patent/JP2686506B2/ja
Application filed by Seiko Instruments Inc filed Critical Seiko Instruments Inc
Publication of DE69214571D1 publication Critical patent/DE69214571D1/de
Application granted granted Critical
Publication of DE69214571T2 publication Critical patent/DE69214571T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6818—Sequencing of polypeptides
    • G01N33/6821—Sequencing of polypeptides involving C-terminal degradation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/12—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by hydrolysis, i.e. solvolysis in general
    • C07K1/128—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by hydrolysis, i.e. solvolysis in general sequencing
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/24—Nuclear magnetic resonance, electron spin resonance or other spin effects or mass spectrometry

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Medicinal Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Urology & Nephrology (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
DE69214571T 1991-08-28 1992-08-26 Verfahren zur Analyse von einem Protein oder einem Peptid Expired - Lifetime DE69214571T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP3217437A JP2686505B2 (ja) 1991-08-28 1991-08-28 タンパク質あるいはペプチドのカルボキシ末端からのアミノ酸配列を決定する方法
JP3300818A JP2686506B2 (ja) 1991-11-15 1991-11-15 タンパク質あるいはペプチドのカルボキシ末端からのアミノ酸配列を決定する方法

Publications (2)

Publication Number Publication Date
DE69214571D1 true DE69214571D1 (de) 1996-11-21
DE69214571T2 DE69214571T2 (de) 1997-02-27

Family

ID=26522024

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69214571T Expired - Lifetime DE69214571T2 (de) 1991-08-28 1992-08-26 Verfahren zur Analyse von einem Protein oder einem Peptid

Country Status (3)

Country Link
US (1) US5521097A (de)
EP (1) EP0529604B1 (de)
DE (1) DE69214571T2 (de)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE427493T1 (de) 1992-05-29 2009-04-15 Univ Rockefeller Verfahren zur bestimmung der folge von peptiden unter verwendung eines massenspektrometers
US5792664A (en) * 1992-05-29 1998-08-11 The Rockefeller University Methods for producing and analyzing biopolymer ladders
US5658739A (en) * 1994-05-10 1997-08-19 The Regents Of The University Of California Method for characterization of the fine structure of protein binding sites
US6291189B1 (en) 1994-05-10 2001-09-18 Carta Proteomics, Inc. Methods for the high-resolution identification of solvent-accessible amide hydrogens in polypeptides or proteins and for characterization of the fine structure of protein binding sites
US5468843A (en) * 1994-09-08 1995-11-21 Perkin-Elmer Acetic anhydride activation for C-terminal protein sequencing
AUPN896396A0 (en) * 1996-03-27 1996-04-26 Garvan Institute Of Medical Research Method for preparing amino acid thiohydantoins
JP3124507B2 (ja) * 1996-05-24 2001-01-15 セイコーインスツルメンツ株式会社 タンパク質あるいはペプチドのカルボキシ末端からのアミノ酸配列を決定する方法
GB9710582D0 (en) 1997-05-22 1997-07-16 Oxford Glycosciences Uk Ltd A method for de novo peptide sequence determination
US5824556A (en) * 1997-06-11 1998-10-20 Tarr; George E. Peptide mass ladders generated using carbon disulfide
US6599707B1 (en) 1998-09-11 2003-07-29 Exsar Corporation Methods for identifying hot-spot residues of binding proteins and small compounds that bind to the same
US6963807B2 (en) 2000-09-08 2005-11-08 Oxford Glycosciences (Uk) Ltd. Automated identification of peptides
JP2002267674A (ja) * 2001-03-14 2002-09-18 Nec Corp 蛋白質あるいはペプチドの分析方法
US6800449B1 (en) 2001-07-13 2004-10-05 Syngenta Participations Ag High throughput functional proteomics
JP4102581B2 (ja) * 2002-03-25 2008-06-18 日本電気株式会社 ペプチドのｃ末端アミノ酸配列解析方法
JP4257492B2 (ja) * 2002-11-29 2009-04-22 日本電気株式会社 ペプチドのｃ末端アミノ酸配列解析方法
JP5497449B2 (ja) * 2007-11-28 2014-05-21 富士フイルム株式会社 生体高分子およびポリペプチドの化学修飾方法
US20150099860A1 (en) * 2013-10-08 2015-04-09 Proteins Easy Corp Methods for separating and purifying endogenous, exogenous and recombinant proteins/peptides from plants and animals using aqueous-free, anhydrous strategies

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2168478A (en) * 1984-11-26 1986-06-18 Scan Limited M Analysis of protein etc using mass spectrometry
US4820648A (en) * 1985-08-21 1989-04-11 Spectros Limited Methods for use in the mass analysis of chemical samples
DE3788169T2 (de) * 1986-06-06 1994-05-11 Genentech Inc Geräte und Verfahren zur Bestimmung der Sequenz von Aminosäuren.
US4861726A (en) * 1988-03-10 1989-08-29 Bio-Affinity Systems, Inc. Method of thioacylation peptide sequencing with acylation of thiazoloinones
JPH0669399B2 (ja) * 1988-03-15 1994-09-07 三菱化成株式会社 カルボキシル末端ペプチドの分取方法
US5185266A (en) * 1991-10-15 1993-02-09 Applied Biosystems, Inc. Cleavage method for acyl thiohydantoins and use of the method in c-terminal peptide sequencing

Also Published As

Publication number Publication date
EP0529604A1 (de) 1993-03-03
DE69214571T2 (de) 1997-02-27
EP0529604B1 (de) 1996-10-16
US5521097A (en) 1996-05-28

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition