DE69132257T2 - Verfahren zur Herstellung eines gereinigten Treponema-Antigens und dessen Verwendung - Google Patents
Verfahren zur Herstellung eines gereinigten Treponema-Antigens und dessen VerwendungInfo
- Publication number
- DE69132257T2 DE69132257T2 DE1991632257 DE69132257T DE69132257T2 DE 69132257 T2 DE69132257 T2 DE 69132257T2 DE 1991632257 DE1991632257 DE 1991632257 DE 69132257 T DE69132257 T DE 69132257T DE 69132257 T2 DE69132257 T2 DE 69132257T2
- Authority
- DE
- Germany
- Prior art keywords
- antigen
- solution
- buffer
- purified
- aqueous medium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/20—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Spirochaetales (O), e.g. Treponema, Leptospira
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Peptides Or Proteins (AREA)
Claims (6)
1. Verfahren zur Herstellung eines gereinigten Treponema-
Antigens, worin das Verfahren das Adsorbieren eines aus
Treponema pallidum stammenden Extrakts an ein
Hydroxyapatit-Gel gefolgt von Elution umfaßt, wobei ein
wäßriges Medium verwendet wird, das aus einem Puffer
besteht, der Octylglucopyranosid enthält.
2. Verfahren gemäß Anspruch 1, worin die Adsorption unter
Verwendung eines wäßrigen Mediums mit pH 5,0 bis 8,0 und
mit einer Ionenstärke von 2 bis 30 mM durchgeführt wird.
3. Verfahren gemäß Anspruch 1, worin die Elution unter
Verwendung eines wäßrigen Mediums mit pH 5,0 bis 11,0
und mit einer Ionenstärke von 10 bis 360 mM durchgeführt
wird.
4. Verfahren gemäß Anspruch 2 oder 3, worin die Elution
unter Verwendung eines wäßrigen Mediums mit entweder
höherem pH oder höherer Ionenstärke als
desjenigen/derjenigen eines für die Adsorption
verwendeten wäßrigen Mediums durchgeführt wird.
5. Verfahren gemäß Anspruch 1, worin das wäßrige Medium
Phosphat-Puffer ist.
6. Verfahren gemäß Anspruch 1, worin die Elution durch eine
Methode mit linearem Gradienten durchgeführt wird.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2067986A JPH0743385B2 (ja) | 1990-03-16 | 1990-03-16 | 梅毒抗原の製造法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE69132257D1 DE69132257D1 (de) | 2000-07-27 |
| DE69132257T2 true DE69132257T2 (de) | 2000-12-21 |
Family
ID=13360812
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE1991631431 Expired - Lifetime DE69131431T2 (de) | 1990-03-16 | 1991-03-14 | Verfahren zur Herstellung einer diagnostischen Reagenz für Syphilis |
| DE1991632257 Expired - Lifetime DE69132257T2 (de) | 1990-03-16 | 1991-03-14 | Verfahren zur Herstellung eines gereinigten Treponema-Antigens und dessen Verwendung |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE1991631431 Expired - Lifetime DE69131431T2 (de) | 1990-03-16 | 1991-03-14 | Verfahren zur Herstellung einer diagnostischen Reagenz für Syphilis |
Country Status (5)
| Country | Link |
|---|---|
| EP (2) | EP0447322B1 (de) |
| JP (1) | JPH0743385B2 (de) |
| AU (1) | AU646023B2 (de) |
| CA (1) | CA2038423C (de) |
| DE (2) | DE69131431T2 (de) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB9314011D0 (en) * | 1993-07-07 | 1993-08-18 | Porton Cambridge Ltd | New diagnostic assay for detection of syphilis |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2233024A1 (en) * | 1973-06-15 | 1975-01-10 | Greyward Lab Interconti | Treponema haemagglutination test - using tanned avian erythrocytes as carrier for ireponema antigen |
| JPS56143955A (en) * | 1980-04-10 | 1981-11-10 | Fujirebio Inc | Method for diagnosis of initial infection of syphilis |
| JPS5871457A (ja) * | 1981-10-23 | 1983-04-28 | Fujirebio Inc | 梅毒検査用試薬の製造法 |
| AU2333084A (en) * | 1982-11-12 | 1984-06-04 | The Regents Of The University Of California | Recombinant dna derived antigens of treponema pallidum |
| JPS6078999A (ja) * | 1983-10-05 | 1985-05-04 | Chemo Sero Therapeut Res Inst | ＨＢｓ抗原の精製方法 |
| US4738932A (en) * | 1985-12-03 | 1988-04-19 | Advanced Polymer Systems, Inc. | Reaginic test for syphilis |
| JPS63101394A (ja) * | 1986-10-17 | 1988-05-06 | Mitsui Toatsu Chem Inc | 生体高分子の分離方法 |
| JPH01297553A (ja) * | 1988-05-25 | 1989-11-30 | Asahi Optical Co Ltd | 液体クロマトグラフィーを用いた蛋白質及び生理活性物質の分離方法 |
| JPH04188066A (ja) * | 1990-11-21 | 1992-07-06 | Sekisui Chem Co Ltd | 抗体の製造方法 |
| JPH04188067A (ja) * | 1990-11-21 | 1992-07-06 | Sekisui Chem Co Ltd | 抗原の製造方法 |
-
1990
- 1990-03-16 JP JP2067986A patent/JPH0743385B2/ja not_active Expired - Lifetime
-
1991
- 1991-03-14 EP EP91400696A patent/EP0447322B1/de not_active Expired - Lifetime
- 1991-03-14 AU AU72908/91A patent/AU646023B2/en not_active Expired
- 1991-03-14 DE DE1991631431 patent/DE69131431T2/de not_active Expired - Lifetime
- 1991-03-14 DE DE1991632257 patent/DE69132257T2/de not_active Expired - Lifetime
- 1991-03-14 EP EP96200278A patent/EP0718627B1/de not_active Expired - Lifetime
- 1991-03-15 CA CA 2038423 patent/CA2038423C/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| EP0447322A2 (de) | 1991-09-18 |
| EP0447322A3 (en) | 1993-09-01 |
| EP0718627B1 (de) | 1999-07-07 |
| EP0718627A1 (de) | 1996-06-26 |
| CA2038423A1 (en) | 1991-09-17 |
| DE69132257D1 (de) | 2000-07-27 |
| CA2038423C (en) | 2002-12-10 |
| JPH0743385B2 (ja) | 1995-05-15 |
| DE69131431D1 (de) | 1999-08-12 |
| AU7290891A (en) | 1991-09-19 |
| JPH03267760A (ja) | 1991-11-28 |
| AU646023B2 (en) | 1994-02-03 |
| EP0447322B1 (de) | 2000-06-21 |
| DE69131431T2 (de) | 1999-10-28 |
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| Date | Code | Title | Description |
|---|---|---|---|
| 8364 | No opposition during term of opposition |