DE69105959T2 - Verfahren zur unterscheidung von nukleinsäuren auf basis von nukleotidverschiedenheiten. - Google Patents
Verfahren zur unterscheidung von nukleinsäuren auf basis von nukleotidverschiedenheiten.Info
- Publication number
- DE69105959T2 DE69105959T2 DE69105959T DE69105959T DE69105959T2 DE 69105959 T2 DE69105959 T2 DE 69105959T2 DE 69105959 T DE69105959 T DE 69105959T DE 69105959 T DE69105959 T DE 69105959T DE 69105959 T2 DE69105959 T2 DE 69105959T2
- Authority
- DE
- Germany
- Prior art keywords
- primer
- dna
- extension product
- extension
- nucleic acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
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- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
- Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
- Y10T436/143333—Saccharide [e.g., DNA, etc.]
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Claims (11)
1. Verfahren zum Nachweis von Polymorphismen auf der Grundlage von Nucleotid-
Unterschieden in statistischen Segmenten von Nucleinsäuren, umfassend:
(a) getrennte Durchführung einer Extensionsreaktion auf einem statistischen
Segment von wenigstens jeweils zwei Nucleinsäuren verschiedenen Ursprungs,
wobei die genannte Reaktion umfaßt:
(i) Zusammenbringen von jeder der obigen Nucleinsäuren mit wenigstens einem
nichtspezifisch zielgerichteten statistischen Oligonucleotid-Primer von größer
als 7 Nucleotiden und Extension des Primers während einer
Extensionsreaktion, wobei für wenigstens eine Nucleinsäure ein statistisches
Extensionsprodukt wenigstens eines Primers synthetisiert wird und
(b) Vergleich der Ergebnisse der getrennt durchgeführten statistischen
Extensionsreaktionen auf Unterschiede.
2. Verfahren nach Anspruch 1, nach Stufe (i) und vor Stufe (b), umfassend die
zusätzlichen Stufen (ii) Dissoziation des Extensionsproduktes von seinem
Komplement und (iii) Amplifizierung des statistischen Nucleinsäurensegments durch
Zusammenbringen des dissoziierten Extensionsproduktes mit einem Primer aus Stufe
(i) und Extension des Primers während einer Extensionsreaktion, wobei ein
Amplifikations-Extensionsprodukt synthetisiert wird, wobei das dissoziierte
Extensionsprodukt als Templat verwendet wird.
3. Verfahren nach Anspruch 2, bei dem die Stufen (ii) und (iii) wenigstens zweimal
durchgeführt werden.
4. Verfahren nach Anspruch 1 (i) oder 2 (iii) unter Verwendung einer Nucleinsäure-
Polymerase und von Nucleosidtriphosphat-Substraten oder ihren Analoga und
Gemischen davon.
5. Verfahren nach Anspruch 1 oder 2, bei dem ein Primer eingesetzt wird.
6. Verfahren nach Anspruch 1 oder 2, bei dem der Primer eine Länge von 9 bis 10
Nucleotiden besitzt.
7. Verfahren nach Anspruch 1 oder 2, bei dem wenigstens ein Primer biotinyliert wird.
8. Verfahren nach Anspruch 4, bei dem die Nucleinsäure-Polymerase eine
DNA-Polymerase ist, und die Nucleosidtriphosphat-Substrate
Desoxyribonucleosidtriphosphat-Substrate sind.
9. Verfahren nach Anspruch 8, bei dem die DNA-Polymerase eine thermostabile
Polymerase ist.
10. Verfahren nach Anspruch 1 oder 2, bei dem ein Unterschied in dem
Extensionsprodukt als Marker zur Konstruktion einer Genkarte verwendet wird.
11. Verfahren nach Anspruch 1 oder 2, bei dem ein Unterschied in dem
Extensionsprodukt als Marker zur Unterscheidung oder Indentifizierung von Individuen
verwendet wird.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US07/494,258 US5126239A (en) | 1990-03-14 | 1990-03-14 | Process for detecting polymorphisms on the basis of nucleotide differences |
| PCT/US1991/000841 WO1991014001A2 (en) | 1990-03-14 | 1991-02-12 | A process for distinguishing nucleic acids on the basis of nucleotide differences |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE69105959D1 DE69105959D1 (de) | 1995-01-26 |
| DE69105959T2 true DE69105959T2 (de) | 1995-05-18 |
Family
ID=23963743
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE69105959T Expired - Fee Related DE69105959T2 (de) | 1990-03-14 | 1991-02-12 | Verfahren zur unterscheidung von nukleinsäuren auf basis von nukleotidverschiedenheiten. |
Country Status (17)
| Country | Link |
|---|---|
| US (1) | US5126239A (de) |
| EP (1) | EP0520039B1 (de) |
| JP (1) | JP2818486B2 (de) |
| AT (1) | ATE115634T1 (de) |
| AU (1) | AU639565B2 (de) |
| BR (1) | BR9106151A (de) |
| CA (1) | CA2078132C (de) |
| DE (1) | DE69105959T2 (de) |
| DK (1) | DK0520039T3 (de) |
| ES (1) | ES2065684T3 (de) |
| FI (1) | FI924049A (de) |
| IL (1) | IL97503A (de) |
| NO (1) | NO304655B1 (de) |
| NZ (1) | NZ237396A (de) |
| PL (1) | PL166895B1 (de) |
| WO (1) | WO1991014001A2 (de) |
| ZA (1) | ZA911891B (de) |
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- 1990-03-14 US US07/494,258 patent/US5126239A/en not_active Expired - Lifetime
-
1991
- 1991-02-12 BR BR919106151A patent/BR9106151A/pt not_active IP Right Cessation
- 1991-02-12 EP EP91908846A patent/EP0520039B1/de not_active Expired - Lifetime
- 1991-02-12 AT AT91908846T patent/ATE115634T1/de not_active IP Right Cessation
- 1991-02-12 DK DK91908846.8T patent/DK0520039T3/da active
- 1991-02-12 PL PL91293188A patent/PL166895B1/pl not_active IP Right Cessation
- 1991-02-12 ES ES91908846T patent/ES2065684T3/es not_active Expired - Lifetime
- 1991-02-12 CA CA002078132A patent/CA2078132C/en not_active Expired - Lifetime
- 1991-02-12 JP JP3508563A patent/JP2818486B2/ja not_active Expired - Fee Related
- 1991-02-12 AU AU77820/91A patent/AU639565B2/en not_active Ceased
- 1991-02-12 WO PCT/US1991/000841 patent/WO1991014001A2/en active IP Right Grant
- 1991-02-12 DE DE69105959T patent/DE69105959T2/de not_active Expired - Fee Related
- 1991-03-11 IL IL9750391A patent/IL97503A/en not_active IP Right Cessation
- 1991-03-12 NZ NZ237396A patent/NZ237396A/en unknown
- 1991-03-14 ZA ZA911891A patent/ZA911891B/xx unknown
-
1992
- 1992-09-10 FI FI924049A patent/FI924049A/fi not_active Application Discontinuation
- 1992-09-11 NO NO922800A patent/NO304655B1/no not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| JP2818486B2 (ja) | 1998-10-30 |
| AU7782091A (en) | 1991-10-10 |
| ZA911891B (en) | 1992-11-25 |
| NO923544D0 (no) | 1992-09-11 |
| EP0520039A1 (de) | 1992-12-30 |
| WO1991014001A2 (en) | 1991-09-19 |
| EP0520039B1 (de) | 1994-12-14 |
| JPH05505528A (ja) | 1993-08-19 |
| AU639565B2 (en) | 1993-07-29 |
| US5126239A (en) | 1992-06-30 |
| IL97503A (en) | 1995-05-26 |
| FI924049A0 (fi) | 1992-09-10 |
| FI924049A (fi) | 1992-09-10 |
| DE69105959D1 (de) | 1995-01-26 |
| ATE115634T1 (de) | 1994-12-15 |
| IL97503A0 (en) | 1992-06-21 |
| DK0520039T3 (da) | 1995-02-20 |
| NZ237396A (en) | 1993-08-26 |
| NO923544L (no) | 1992-09-11 |
| WO1991014001A3 (en) | 1991-10-31 |
| PL293188A1 (en) | 1992-11-02 |
| CA2078132A1 (en) | 1991-09-15 |
| ES2065684T3 (es) | 1995-02-16 |
| BR9106151A (pt) | 1993-03-09 |
| CA2078132C (en) | 1999-08-31 |
| PL166895B1 (pl) | 1995-06-30 |
| NO304655B1 (no) | 1999-01-25 |
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