DE69033855T2 - Modifizierte biolumineszierende proteine und deren verwendung - Google Patents

Modifizierte biolumineszierende proteine und deren verwendung

Info

Publication number
DE69033855T2
DE69033855T2 DE69033855T DE69033855T DE69033855T2 DE 69033855 T2 DE69033855 T2 DE 69033855T2 DE 69033855 T DE69033855 T DE 69033855T DE 69033855 T DE69033855 T DE 69033855T DE 69033855 T2 DE69033855 T2 DE 69033855T2
Authority
DE
Germany
Prior art keywords
analyte
interaction site
bioluminescent
produced
bioluminescent proteins
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69033855T
Other languages
English (en)
Other versions
DE69033855D1 (de
Inventor
Keith Campbell
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University College Cardiff Consultants Ltd
Original Assignee
University of Wales College of Medicine
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Wales College of Medicine filed Critical University of Wales College of Medicine
Application granted granted Critical
Publication of DE69033855D1 publication Critical patent/DE69033855D1/de
Publication of DE69033855T2 publication Critical patent/DE69033855T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K49/00—Preparations for testing in vivo
    • A61K49/001—Preparation for luminescence or biological staining
    • A61K49/0013—Luminescence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43536—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from worms
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43595—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from coelenteratae, e.g. medusae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
DE69033855T 1989-07-22 1990-07-23 Modifizierte biolumineszierende proteine und deren verwendung Expired - Fee Related DE69033855T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB898916806A GB8916806D0 (en) 1989-07-22 1989-07-22 Modified proteins
PCT/GB1990/001131 WO1991001305A1 (en) 1989-07-22 1990-07-23 Modified bioluminescent proteins and their use

Publications (2)

Publication Number Publication Date
DE69033855D1 DE69033855D1 (de) 2001-12-20
DE69033855T2 true DE69033855T2 (de) 2002-07-11

Family

ID=10660479

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69033855T Expired - Fee Related DE69033855T2 (de) 1989-07-22 1990-07-23 Modifizierte biolumineszierende proteine und deren verwendung

Country Status (10)

Country Link
EP (2) EP1097992A3 (de)
JP (2) JP3375337B2 (de)
AT (1) ATE208792T1 (de)
AU (1) AU6054590A (de)
CA (1) CA2064766A1 (de)
DE (1) DE69033855T2 (de)
DK (1) DK0484369T3 (de)
ES (1) ES2167307T3 (de)
GB (1) GB8916806D0 (de)
WO (1) WO1991001305A1 (de)

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JP2666561B2 (ja) * 1990-03-27 1997-10-22 キッコーマン株式会社 変異型ホタルルシフェラーゼ、変異型ホタルルシフェラーゼ遺伝子、新規な組み換え体ｄｎａ及び変異型ホタルルシフェラーゼの製造法
GB9126650D0 (en) * 1991-12-16 1992-02-12 Creighton Andrew M Further improvements relating to the radiolabelling of proteins
CA2169298A1 (en) * 1993-09-10 1995-03-16 Martin Chalfie Uses of green fluorescent protein
US5650135A (en) * 1994-07-01 1997-07-22 The Board Of Trustees Of The Leland Stanford Junior University Non-invasive localization of a light-emitting conjugate in a mammal
US6217847B1 (en) 1994-07-01 2001-04-17 The Board Of Trustees Of The Leland Stanford Junior University Non-invasive localization of a light-emitting conjugate in a mammal
US7255851B2 (en) 1994-07-01 2007-08-14 The Board Of Trustees Of The Leland Stanford Junior University Non-invasive localization of a light-emitting conjugate in a mammal
IL115177A0 (en) * 1994-09-16 1995-12-31 Immunomedics Inc Phosphorus-32 labeling of antibodies for cancer therapy
US5625048A (en) 1994-11-10 1997-04-29 The Regents Of The University Of California Modified green fluorescent proteins
US5777079A (en) * 1994-11-10 1998-07-07 The Regents Of The University Of California Modified green fluorescent proteins
US5958713A (en) * 1995-01-31 1999-09-28 Novo Nordisk A/S Method of detecting biologically active substances by using green fluorescent protein
GB9504446D0 (en) * 1995-03-06 1995-04-26 Medical Res Council Improvements in or relating to gene expression
EP1820517A3 (de) * 1995-11-17 2009-10-21 The Board Of Trustees Of The Leland Stanford Junior University Nichtinvasive Lokalisierung eines lichtemittierenden Konjugats bei einem Säugetier
US6803188B1 (en) * 1996-01-31 2004-10-12 The Regents Of The University Of California Tandem fluorescent protein constructs
US6900304B2 (en) 1996-01-31 2005-05-31 The Regents Of The University Of California Emission ratiometric indicators of phosphorylation
US8349602B1 (en) 1996-04-19 2013-01-08 Xenogen Corporation Biodetectors targeted to specific ligands
US5912137A (en) 1996-07-16 1999-06-15 The Regents Of The University Of California Assays for protein kinases using fluorescent
EP0915989B1 (de) * 1996-07-16 2010-06-02 The Regents Of The University Of California Tests auf proteinkinasen mit fluoreszierenden proteinsubstraten
US5925558A (en) * 1996-07-16 1999-07-20 The Regents Of The University Of California Assays for protein kinases using fluorescent protein substrates
US6593135B2 (en) 1996-08-16 2003-07-15 The State Of Oregon, Acting By And Through The State Board Of Higher Education On Behalf Of The University Of Oregon Long wavelength engineered fluorescent proteins
US6124128A (en) * 1996-08-16 2000-09-26 The Regents Of The University Of California Long wavelength engineered fluorescent proteins
US5976796A (en) * 1996-10-04 1999-11-02 Loma Linda University Construction and expression of renilla luciferase and green fluorescent protein fusion genes
US6197928B1 (en) 1997-03-14 2001-03-06 The Regents Of The University Of California Fluorescent protein sensors for detection of analytes
US5998204A (en) * 1997-03-14 1999-12-07 The Regents Of The University Of California Fluorescent protein sensors for detection of analytes
DK0986753T4 (da) * 1997-04-07 2005-12-19 Bioimage As Fremgangsmåde til uddragning af kvantitativ information, hvad angår en påvirkning af et cellulært respons
US6046925A (en) * 1997-04-14 2000-04-04 The Regents Of The University Of California Photochromic fluorescent proteins and optical memory storage devices based on fluorescent proteins
FR2764387B1 (fr) * 1997-06-05 1999-07-23 Centre Nat Rech Scient Utilisation d'une proteine fluorescente pour la detection d'interactions entre une proteine cible et son ligand
DE19737562A1 (de) * 1997-08-28 1999-05-06 Otogene Biotechnologische Fors Verfahren zur Identifizierung von Wechselwirkungen zwischen Proteinen bzw. Peptiden
US6495664B1 (en) 1998-07-24 2002-12-17 Aurora Biosciences Corporation Fluorescent protein sensors of post-translational modifications
EP0984070A1 (de) * 1998-08-31 2000-03-08 Boehringer Ingelheim International GmbH Verfahren zur Auffindung von Verbindungen für die Behandlung von Krankheiten die assoziert sind mit C-Jun vermittelte Apoptosis
EP1121593A2 (de) * 1998-10-15 2001-08-08 BioImage A/S Verfahren zur ableitung quantitativer informationen über einen einfluss auf eine zelluläre respons
EP1146888A2 (de) * 1998-10-15 2001-10-24 BioImage A/S Spezifische therapeutischeinverventionen durch interferenz aufgrund von umverteilung und/oder targetting
US6410255B1 (en) 1999-05-05 2002-06-25 Aurora Biosciences Corporation Optical probes and assays
US6573059B1 (en) 1999-06-04 2003-06-03 Rmf Dictagene S.A. Use of the regulatory subunit of the camp dependent protein kinase (PKA) from dictyostelium for camp measurements
DE60135652D1 (de) * 2000-06-01 2008-10-16 Pasteur Institut Chimäres GPF-Aequorin als bioluminiszenter Ca++ Marker auf Einzelzellniveau
JP3829252B2 (ja) 2001-06-08 2006-10-04 独立行政法人理化学研究所 蛍光蛋白質
WO2003083112A1 (fr) * 2002-04-01 2003-10-09 Japan Science And Technology Agency Population de cellules pourvues de codes d'identification et procedes pour le criblage de population de cellules
DE10342670A1 (de) * 2003-09-16 2005-04-21 Bayer Healthcare Ag Isoliertes Photoprotein mtClytin, sowie dessen Verwendung
EP1673459B1 (de) * 2003-10-10 2012-06-27 Promega Corporation Luciferase biosensor
JP2005120005A (ja) * 2003-10-16 2005-05-12 Sekisui Chem Co Ltd 有機蛍光物質及び有機蛍光物質の製造方法
EP2327768B1 (de) 2006-04-03 2015-09-09 Promega Corporation Permutierte und nichtpermutierte Luciferase-Biosensoren
JP6110591B2 (ja) 2008-05-19 2017-04-05 プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ ｃＡＭＰのためのルシフェラーゼバイオセンサー
US9290794B2 (en) 2010-05-11 2016-03-22 Promega Corporation Mutant protease biosensors with enhanced detection characteristics
WO2011143339A1 (en) 2010-05-11 2011-11-17 Promega Corporation Mutant protease biosensors with enhanced detection characteristics

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2129553B (en) * 1982-09-10 1986-06-18 Welsh Nat School Med Immunological procedure for detecting or quantifying substances
ES8607563A1 (es) * 1983-10-13 1986-06-01 Univ Georgia Res Found Un metodo para detectar una interaccion ligante
JPS6439990A (en) * 1987-08-05 1989-02-10 Chisso Corp Fused gene by bonding aequorin gene to functional gene

Also Published As

Publication number Publication date
DE69033855D1 (de) 2001-12-20
EP0484369B1 (de) 2001-11-14
ES2167307T3 (es) 2002-05-16
JPH05501862A (ja) 1993-04-08
EP1097992A2 (de) 2001-05-09
EP0484369A1 (de) 1992-05-13
WO1991001305A1 (en) 1991-02-07
DK0484369T3 (da) 2002-03-11
CA2064766A1 (en) 1991-01-23
GB8916806D0 (en) 1989-09-06
ATE208792T1 (de) 2001-11-15
AU6054590A (en) 1991-02-22
JP2003102491A (ja) 2003-04-08
EP1097992A3 (de) 2001-05-16
JP3375337B2 (ja) 2003-02-10

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8328 Change in the person/name/address of the agent

Representative=s name: ZEITLER, VOLPERT, KANDLBINDER, 80539 MUENCHEN

8327 Change in the person/name/address of the patent owner

Owner name: UNIVERSITY COLLEGE CARDIFF CONSULTANTS LTD., CARDI

8339 Ceased/non-payment of the annual fee