DE69028526T2 - Verfahren zur vorwahl des geschlechts der nachkommenschaft - Google Patents
Verfahren zur vorwahl des geschlechts der nachkommenschaftInfo
- Publication number
- DE69028526T2 DE69028526T2 DE69028526T DE69028526T DE69028526T2 DE 69028526 T2 DE69028526 T2 DE 69028526T2 DE 69028526 T DE69028526 T DE 69028526T DE 69028526 T DE69028526 T DE 69028526T DE 69028526 T2 DE69028526 T2 DE 69028526T2
- Authority
- DE
- Germany
- Prior art keywords
- sperm
- sex
- dna
- carrier fluid
- mammal
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 238000000034 method Methods 0.000 title claims abstract description 35
- 210000004027 cell Anatomy 0.000 claims abstract description 21
- 241000283973 Oryctolagus cuniculus Species 0.000 claims abstract description 11
- 238000010186 staining Methods 0.000 claims abstract description 10
- 230000035899 viability Effects 0.000 claims abstract description 9
- 108020004414 DNA Proteins 0.000 claims description 39
- 239000012530 fluid Substances 0.000 claims description 17
- 235000013601 eggs Nutrition 0.000 claims description 13
- 241000124008 Mammalia Species 0.000 claims description 11
- 241000894007 species Species 0.000 claims description 6
- 108091003079 Bovine Serum Albumin Proteins 0.000 claims description 5
- 239000004606 Fillers/Extenders Substances 0.000 claims description 5
- 229940098773 bovine serum albumin Drugs 0.000 claims description 5
- 238000011534 incubation Methods 0.000 claims description 5
- 241000283690 Bos taurus Species 0.000 claims description 4
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 claims description 4
- 210000002969 egg yolk Anatomy 0.000 claims description 4
- 238000002347 injection Methods 0.000 claims description 4
- 239000007924 injection Substances 0.000 claims description 4
- 239000002953 phosphate buffered saline Substances 0.000 claims description 4
- 102000002322 Egg Proteins Human genes 0.000 claims description 3
- 108010000912 Egg Proteins Proteins 0.000 claims description 3
- 210000000349 chromosome Anatomy 0.000 claims description 3
- 235000013345 egg yolk Nutrition 0.000 claims description 3
- 231100000252 nontoxic Toxicity 0.000 claims description 3
- 230000003000 nontoxic effect Effects 0.000 claims description 3
- 238000012360 testing method Methods 0.000 claims description 3
- 230000005284 excitation Effects 0.000 claims description 2
- 239000000243 solution Substances 0.000 claims description 2
- 239000000725 suspension Substances 0.000 claims description 2
- 238000003744 In vitro fertilisation Methods 0.000 claims 1
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 1
- 239000007788 liquid Substances 0.000 claims 1
- 230000001105 regulatory effect Effects 0.000 claims 1
- 210000002593 Y chromosome Anatomy 0.000 abstract description 12
- 210000001766 X chromosome Anatomy 0.000 abstract description 10
- 210000004291 uterus Anatomy 0.000 abstract description 4
- 241000282898 Sus scrofa Species 0.000 abstract 2
- 235000015097 nutrients Nutrition 0.000 abstract 1
- 102000053602 DNA Human genes 0.000 description 35
- 230000004720 fertilization Effects 0.000 description 18
- 210000004940 nucleus Anatomy 0.000 description 13
- 239000000975 dye Substances 0.000 description 8
- BFMYDTVEBKDAKJ-UHFFFAOYSA-L disodium;(2',7'-dibromo-3',6'-dioxido-3-oxospiro[2-benzofuran-1,9'-xanthene]-4'-yl)mercury;hydrate Chemical compound O.[Na+].[Na+].O1C(=O)C2=CC=CC=C2C21C1=CC(Br)=C([O-])C([Hg])=C1OC1=C2C=C(Br)C([O-])=C1 BFMYDTVEBKDAKJ-UHFFFAOYSA-L 0.000 description 7
- 210000000582 semen Anatomy 0.000 description 7
- 241001465754 Metazoa Species 0.000 description 5
- 244000309464 bull Species 0.000 description 4
- 230000009027 insemination Effects 0.000 description 4
- 238000005259 measurement Methods 0.000 description 4
- 230000003287 optical effect Effects 0.000 description 4
- 108090000623 proteins and genes Proteins 0.000 description 4
- 238000012950 reanalysis Methods 0.000 description 4
- JABNPSKWVNCGMX-UHFFFAOYSA-N 2-(4-ethoxyphenyl)-6-[6-(4-methylpiperazin-1-yl)-1h-benzimidazol-2-yl]-1h-benzimidazole;trihydrochloride Chemical compound Cl.Cl.Cl.C1=CC(OCC)=CC=C1C1=NC2=CC=C(C=3NC4=CC(=CC=C4N=3)N3CCN(C)CC3)C=C2N1 JABNPSKWVNCGMX-UHFFFAOYSA-N 0.000 description 3
- 102000011022 Chorionic Gonadotropin Human genes 0.000 description 3
- 108010062540 Chorionic Gonadotropin Proteins 0.000 description 3
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 3
- 238000004163 cytometry Methods 0.000 description 3
- 238000000684 flow cytometry Methods 0.000 description 3
- 239000007850 fluorescent dye Substances 0.000 description 3
- 229940084986 human chorionic gonadotropin Drugs 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 210000003765 sex chromosome Anatomy 0.000 description 3
- 241000700112 Chinchilla Species 0.000 description 2
- 241000699800 Cricetinae Species 0.000 description 2
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 2
- 235000013365 dairy product Nutrition 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 230000035558 fertility Effects 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 210000001161 mammalian embryo Anatomy 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 239000004094 surface-active agent Substances 0.000 description 2
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 2
- WEEMDRWIKYCTQM-UHFFFAOYSA-N 2,6-dimethoxybenzenecarbothioamide Chemical compound COC1=CC=CC(OC)=C1C(N)=S WEEMDRWIKYCTQM-UHFFFAOYSA-N 0.000 description 1
- UPXRTVAIJMUAQR-UHFFFAOYSA-N 4-(9h-fluoren-9-ylmethoxycarbonylamino)-1-[(2-methylpropan-2-yl)oxycarbonyl]pyrrolidine-2-carboxylic acid Chemical compound C1C(C(O)=O)N(C(=O)OC(C)(C)C)CC1NC(=O)OCC1C2=CC=CC=C2C2=CC=CC=C21 UPXRTVAIJMUAQR-UHFFFAOYSA-N 0.000 description 1
- 208000037088 Chromosome Breakage Diseases 0.000 description 1
- 230000004568 DNA-binding Effects 0.000 description 1
- LLQPHQFNMLZJMP-UHFFFAOYSA-N Fentrazamide Chemical compound N1=NN(C=2C(=CC=CC=2)Cl)C(=O)N1C(=O)N(CC)C1CCCCC1 LLQPHQFNMLZJMP-UHFFFAOYSA-N 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- JOCBASBOOFNAJA-UHFFFAOYSA-N N-tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid Chemical compound OCC(CO)(CO)NCCS(O)(=O)=O JOCBASBOOFNAJA-UHFFFAOYSA-N 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 241000282887 Suidae Species 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- NOSIYYJFMPDDSA-UHFFFAOYSA-N acepromazine Chemical compound C1=C(C(C)=O)C=C2N(CCCN(C)C)C3=CC=CC=C3SC2=C1 NOSIYYJFMPDDSA-UHFFFAOYSA-N 0.000 description 1
- 229960005054 acepromazine Drugs 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- XKRFYHLGVUSROY-UHFFFAOYSA-N argon Substances [Ar] XKRFYHLGVUSROY-UHFFFAOYSA-N 0.000 description 1
- 229910052786 argon Inorganic materials 0.000 description 1
- -1 argon ion Chemical class 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000027455 binding Effects 0.000 description 1
- 244000309466 calf Species 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 230000003833 cell viability Effects 0.000 description 1
- 230000002759 chromosomal effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 239000008121 dextrose Substances 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 210000002257 embryonic structure Anatomy 0.000 description 1
- 238000009313 farming Methods 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- BCQZXOMGPXTTIC-UHFFFAOYSA-N halothane Chemical compound FC(F)(F)C(Cl)Br BCQZXOMGPXTTIC-UHFFFAOYSA-N 0.000 description 1
- 229960003132 halothane Drugs 0.000 description 1
- 229960004184 ketamine hydrochloride Drugs 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 238000000520 microinjection Methods 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 210000000472 morula Anatomy 0.000 description 1
- 230000004899 motility Effects 0.000 description 1
- 238000011587 new zealand white rabbit Methods 0.000 description 1
- 230000016087 ovulation Effects 0.000 description 1
- 210000004681 ovum Anatomy 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 230000035515 penetration Effects 0.000 description 1
- 229940056360 penicillin g Drugs 0.000 description 1
- 235000015277 pork Nutrition 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 229960002385 streptomycin sulfate Drugs 0.000 description 1
- 235000000346 sugar Nutrition 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 210000001215 vagina Anatomy 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/48—Reproductive organs
- A61K35/52—Sperm; Prostate; Seminal fluid; Leydig cells of testes
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Biomedical Technology (AREA)
- Cell Biology (AREA)
- Chemical & Material Sciences (AREA)
- Reproductive Health (AREA)
- Medicinal Chemistry (AREA)
- Biotechnology (AREA)
- Hematology (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Pharmacology & Pharmacy (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Food Science & Technology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Developmental Biology & Embryology (AREA)
- Virology (AREA)
- Zoology (AREA)
- Physics & Mathematics (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Electrically Operated Instructional Devices (AREA)
- Input Circuits Of Receivers And Coupling Of Receivers And Audio Equipment (AREA)
- Electrical Discharge Machining, Electrochemical Machining, And Combined Machining (AREA)
- Crystals, And After-Treatments Of Crystals (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US34966989A | 1989-05-10 | 1989-05-10 | |
| PCT/US1990/002324 WO1990013303A1 (en) | 1989-05-10 | 1990-04-30 | Method to preselect the sex of offspring |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE69028526D1 DE69028526D1 (de) | 1996-10-17 |
| DE69028526T2 true DE69028526T2 (de) | 1997-02-06 |
Family
ID=23373453
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE69028526T Expired - Lifetime DE69028526T2 (de) | 1989-05-10 | 1990-04-30 | Verfahren zur vorwahl des geschlechts der nachkommenschaft |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US5135759A (enExample) |
| EP (1) | EP0471758B1 (enExample) |
| JP (1) | JP2552582B2 (enExample) |
| AT (1) | ATE142788T1 (enExample) |
| AU (1) | AU623016B2 (enExample) |
| CA (1) | CA2055494C (enExample) |
| DE (1) | DE69028526T2 (enExample) |
| DK (1) | DK0471758T3 (enExample) |
| ES (1) | ES2091823T3 (enExample) |
| WO (1) | WO1990013303A1 (enExample) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7586604B2 (en) | 1997-01-31 | 2009-09-08 | Xy, Inc. | Optical apparatus |
| US7629113B2 (en) | 1997-12-31 | 2009-12-08 | Xy, Inc | Multiple sexed embryo production system for bovine mammals |
| US7723116B2 (en) | 2003-05-15 | 2010-05-25 | Xy, Inc. | Apparatus, methods and processes for sorting particles and for providing sex-sorted animal sperm |
| US7820425B2 (en) | 1999-11-24 | 2010-10-26 | Xy, Llc | Method of cryopreserving selected sperm cells |
| US7855078B2 (en) | 2002-08-15 | 2010-12-21 | Xy, Llc | High resolution flow cytometer |
| US8137967B2 (en) | 2000-11-29 | 2012-03-20 | Xy, Llc | In-vitro fertilization systems with spermatozoa separated into X-chromosome and Y-chromosome bearing populations |
| US8486618B2 (en) | 2002-08-01 | 2013-07-16 | Xy, Llc | Heterogeneous inseminate system |
Families Citing this family (129)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5346990A (en) * | 1987-04-08 | 1994-09-13 | Cytogam, Inc. | Sex-associated membrane proteins and methods for increasing the probability that offspring will be of a desired sex |
| ATE127695T1 (de) * | 1989-05-12 | 1995-09-15 | Cytogam Inc | Mit dem geschlecht assoziierte membranproteine und methoden zur erhöhung der wahrscheinlichkeit, dass die nachkommenschaft das gewünschte geschlecht besitzt. |
| JP2992298B2 (ja) * | 1989-05-12 | 1999-12-20 | サイトガム，インコーポレイテッド | 性関連膜タンパク質および子が所望の性を有する確率を増大させるための方法 |
| US5648468A (en) * | 1990-03-09 | 1997-07-15 | Spaulding; Glenn F. | Process for identifying sex associated egg proteins and methods for increasing the probability that the avian offspring will be the desired sex |
| US5919621A (en) * | 1991-10-24 | 1999-07-06 | Brown; David B. | Methods for diagnosing human male infertility |
| US5770363A (en) * | 1991-10-24 | 1998-06-23 | Brown; David B. | Methods for diagnosing human male infertility |
| FR2699678A1 (fr) * | 1992-12-23 | 1994-06-24 | Unceia | Procédé cytométrique de séparation de spermatozoïdes X et Y en fonction de leur contenu en ADN. |
| JPH08507370A (ja) * | 1993-01-06 | 1996-08-06 | ベーリンガー マンハイム コーポレーション | 生存できない細胞の蛍光検出 |
| DE69834527T2 (de) | 1997-07-01 | 2007-05-10 | VLP Watertown Limited Partnership, Watertown | Verfahren zur Geschlechtsbestimmung von Säuger-Nachkommenschaft |
| RU2442324C2 (ru) * | 1997-12-31 | 2012-02-20 | Кси, Ллк | Способ получения нескольких эмбрионов и млекопитающего заданного пола |
| US6149867A (en) | 1997-12-31 | 2000-11-21 | Xy, Inc. | Sheath fluids and collection systems for sex-specific cytometer sorting of sperm |
| EP1062494B1 (en) | 1998-02-20 | 2015-06-24 | Xy, Llc | A vibratory system for a sorting flow cytometer |
| WO2000006193A1 (en) * | 1998-07-30 | 2000-02-10 | Xy, Inc. | Equine system for non-surgical artificial insemination |
| US7024316B1 (en) * | 1999-10-21 | 2006-04-04 | Dakocytomation Colorado, Inc. | Transiently dynamic flow cytometer analysis system |
| US6263745B1 (en) | 1999-12-03 | 2001-07-24 | Xy, Inc. | Flow cytometer nozzle and flow cytometer sample handling methods |
| IT1317724B1 (it) * | 2000-01-14 | 2003-07-15 | Istituto Sperimentale Italiano | Procedimento per la produzione di embrioni non umani di sessopredeterminato ad alto valore genetico. |
| DK2258170T3 (en) | 2000-05-09 | 2017-10-16 | Xy Llc | X-chromosome-bearing and Y-chromosome-bearing populations of high purity spermatozoa |
| NZ537680A (en) | 2000-10-05 | 2007-01-26 | Xy Inc | System of hysteroscopic insemination |
| US7713687B2 (en) | 2000-11-29 | 2010-05-11 | Xy, Inc. | System to separate frozen-thawed spermatozoa into x-chromosome bearing and y-chromosome bearing populations |
| GB0031744D0 (en) * | 2000-12-28 | 2001-02-07 | Pig Improvement Co Uk Ltd | Methods |
| CN1313061C (zh) * | 2002-03-11 | 2007-05-02 | 刘凤鸣 | 一种动物精子的性别分选方法 |
| EP1542529A4 (en) * | 2002-07-22 | 2006-08-09 | Xy Inc | SYSTEM FOR TREATING SPERMATIC CELL |
| US11243494B2 (en) | 2002-07-31 | 2022-02-08 | Abs Global, Inc. | Multiple laminar flow-based particle and cellular separation with laser steering |
| BRPI0313163B1 (pt) | 2002-08-01 | 2015-11-17 | Univ Colorado State | sistema de separação de células espermáticas a baixa pressão |
| US7169548B2 (en) | 2002-09-13 | 2007-01-30 | Xy, Inc. | Sperm cell processing and preservation systems |
| US20040142384A1 (en) * | 2003-01-16 | 2004-07-22 | Cohen Barb Ariel | Magnetic separator |
| DK2305172T3 (en) * | 2003-03-28 | 2016-08-22 | Inguran Llc | DEVICE AND PROCEDURES TO PROVIDE SEXUAL ANIMAL SEED |
| AU2004226343B2 (en) * | 2003-03-28 | 2008-10-09 | Inguran, Llc | Process for the staining of sperm |
| AU2004285960A1 (en) | 2003-10-30 | 2005-05-12 | Cytonome/St, Llc | Multilayer hydrodynamic sheath flow structure |
| WO2005042721A2 (en) * | 2003-10-31 | 2005-05-12 | Abs Global, Inc. | A method for altering the gender ratio of offspring in mammals by manipulation of spermatozoa |
| NZ530972A (en) * | 2004-02-05 | 2005-04-29 | Embrionics Ltd | A method and apparatus for orientating and selecting cells |
| PL2151243T3 (pl) | 2004-03-29 | 2013-03-29 | Inguran Llc | Zawiesiny plemników do sortowania na populacje wzbogacone w niosące chromosom X lub Y |
| CN101238369B (zh) * | 2004-03-29 | 2012-07-04 | 英格朗公司 | 调节细胞内和/或细胞外氧化/还原反应的组合物在精子染色或分选方法中的用途 |
| MX2007000888A (es) | 2004-07-22 | 2007-04-02 | Monsanto Technology Llc | Procedimiento para enriquecer una poblacion de celulas de esperma. |
| DK1771729T3 (en) * | 2004-07-27 | 2015-11-23 | Beckman Coulter Inc | Improving flowcytometridiskrimination using geometric transformation |
| WO2006029653A1 (en) * | 2004-09-16 | 2006-03-23 | Masterrind Gmbh | Method for preservation for gender specific selection of mammalian spermatozoa |
| US7355696B2 (en) | 2005-02-01 | 2008-04-08 | Arryx, Inc | Method and apparatus for sorting cells |
| US20060172315A1 (en) * | 2005-02-01 | 2006-08-03 | Anderson Amy L | Methods for staining cells for identification and sorting |
| CA2598805A1 (en) * | 2005-02-24 | 2006-08-31 | Biocern, Inc. | Sperm cell separation methods and compositions |
| WO2006116627A1 (en) * | 2005-04-28 | 2006-11-02 | Genetics & Ivf Institute | Methods for reducing the percentage of abnormal gametes |
| US7618770B2 (en) | 2005-07-29 | 2009-11-17 | Xy, Inc. | Methods and apparatus for reducing protein content in sperm cell extenders |
| US20080118908A1 (en) * | 2006-11-17 | 2008-05-22 | Dmitri Dozortsev | Method of selecting a sperm cell based on its dna content |
| US7947457B2 (en) * | 2007-01-08 | 2011-05-24 | The Invention Science Fund I, Llc | Systems for genome selection |
| US7985578B2 (en) | 2007-01-08 | 2011-07-26 | The Invention Science Fund I, Llc | Systems for genome selection |
| US7947455B2 (en) * | 2007-01-08 | 2011-05-24 | The Invention Science Fund I, Llc | System for genome selection |
| US7709245B2 (en) * | 2007-01-08 | 2010-05-04 | The Invention Science Fund I, Llc | Systems for genome selection |
| US8521440B2 (en) * | 2007-01-08 | 2013-08-27 | The Invention Science Fund I, Llc | Systems for genome selection |
| US8012740B2 (en) * | 2007-01-08 | 2011-09-06 | The Invention Science Fund I, Llc | Systems for genome selection |
| US8003371B2 (en) * | 2007-01-08 | 2011-08-23 | The Invention Science Fund I, Llc | Systems for genome selection |
| US20080167851A1 (en) * | 2007-01-08 | 2008-07-10 | Searete Llc, A Limited Liability Corporation Of The State Of Delaware | Systems for genome selection |
| US7718418B2 (en) | 2007-01-08 | 2010-05-18 | The Invention Science Fund I, Llc | Systems for genome selection |
| US7713728B2 (en) * | 2007-01-08 | 2010-05-11 | The Invention Sciencefund I, Llc | Systems for genome selection |
| US7709244B2 (en) * | 2007-01-08 | 2010-05-04 | The Invention Science Fund I, Llc | Systems for genome selection |
| US7648823B2 (en) * | 2007-01-08 | 2010-01-19 | Searete Llc | Systems for genome selection |
| US8101426B2 (en) * | 2007-03-02 | 2012-01-24 | Icyt Mission Technology, Inc. | System and method for the measurement of multiple fluorescence emissions in a flow cytometry system |
| CL2008002118A1 (es) * | 2007-07-19 | 2008-12-19 | Xy Llc | Metodo para la produccion de un embrion equino de sexo seleccionado mediante la inyeccion intracitoplasmatica de esperma utilizando espermatozoides de equino seleccionados por sexo. |
| CN101821623A (zh) * | 2007-07-23 | 2010-09-01 | 安德罗珍尼克斯有限公司 | 用于精子性别选择的材料和方法 |
| CA2701170A1 (en) | 2007-09-29 | 2009-08-27 | Timothy James Williams | Composition and method to modify sperm function and increase male gender ratio in mammals |
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| GB2145112B (en) * | 1983-04-27 | 1987-02-18 | Milk Marketing Board | Sorting living spermatozoa |
| AU639208B2 (en) * | 1989-05-12 | 1993-07-22 | Cytogam Inc. | Sex-associated membrane proteins and methods for increasing the probability that offspring will be of a desired sex |
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1990
- 1990-04-30 DK DK90907724.0T patent/DK0471758T3/da active
- 1990-04-30 ES ES90907724T patent/ES2091823T3/es not_active Expired - Lifetime
- 1990-04-30 AT AT90907724T patent/ATE142788T1/de not_active IP Right Cessation
- 1990-04-30 AU AU56642/90A patent/AU623016B2/en not_active Expired
- 1990-04-30 WO PCT/US1990/002324 patent/WO1990013303A1/en not_active Ceased
- 1990-04-30 DE DE69028526T patent/DE69028526T2/de not_active Expired - Lifetime
- 1990-04-30 EP EP90907724A patent/EP0471758B1/en not_active Expired - Lifetime
- 1990-04-30 CA CA002055494A patent/CA2055494C/en not_active Expired - Lifetime
- 1990-04-30 JP JP2507796A patent/JP2552582B2/ja not_active Expired - Lifetime
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1991
- 1991-04-26 US US07/692,958 patent/US5135759A/en not_active Expired - Lifetime
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| US7586604B2 (en) | 1997-01-31 | 2009-09-08 | Xy, Inc. | Optical apparatus |
| US7929137B2 (en) | 1997-01-31 | 2011-04-19 | Xy, Llc | Optical apparatus |
| US7629113B2 (en) | 1997-12-31 | 2009-12-08 | Xy, Inc | Multiple sexed embryo production system for bovine mammals |
| US7820425B2 (en) | 1999-11-24 | 2010-10-26 | Xy, Llc | Method of cryopreserving selected sperm cells |
| US8137967B2 (en) | 2000-11-29 | 2012-03-20 | Xy, Llc | In-vitro fertilization systems with spermatozoa separated into X-chromosome and Y-chromosome bearing populations |
| US9879221B2 (en) | 2000-11-29 | 2018-01-30 | Xy, Llc | Method of in-vitro fertilization with spermatozoa separated into X-chromosome and Y-chromosome bearing populations |
| US8486618B2 (en) | 2002-08-01 | 2013-07-16 | Xy, Llc | Heterogeneous inseminate system |
| US7855078B2 (en) | 2002-08-15 | 2010-12-21 | Xy, Llc | High resolution flow cytometer |
| US7723116B2 (en) | 2003-05-15 | 2010-05-25 | Xy, Inc. | Apparatus, methods and processes for sorting particles and for providing sex-sorted animal sperm |
Also Published As
| Publication number | Publication date |
|---|---|
| EP0471758B1 (en) | 1996-09-11 |
| DE69028526D1 (de) | 1996-10-17 |
| AU5664290A (en) | 1990-11-29 |
| ES2091823T3 (es) | 1996-11-16 |
| DK0471758T3 (enExample) | 1997-03-10 |
| AU623016B2 (en) | 1992-04-30 |
| JPH04501364A (ja) | 1992-03-12 |
| ATE142788T1 (de) | 1996-09-15 |
| HK1000074A1 (en) | 1997-11-14 |
| JP2552582B2 (ja) | 1996-11-13 |
| EP0471758A1 (en) | 1992-02-26 |
| WO1990013303A1 (en) | 1990-11-15 |
| EP0471758A4 (en) | 1992-05-06 |
| US5135759A (en) | 1992-08-04 |
| CA2055494C (en) | 2000-01-18 |
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