BR112012025022A2 - "método de redução da fragmentação de dna em uma amostra de espermatozoides separados" - Google Patents

"método de redução da fragmentação de dna em uma amostra de espermatozoides separados"

Info

Publication number
BR112012025022A2
BR112012025022A2 BR112012025022A BR112012025022A BR112012025022A2 BR 112012025022 A2 BR112012025022 A2 BR 112012025022A2 BR 112012025022 A BR112012025022 A BR 112012025022A BR 112012025022 A BR112012025022 A BR 112012025022A BR 112012025022 A2 BR112012025022 A2 BR 112012025022A2
Authority
BR
Brazil
Prior art keywords
dna fragmentation
samples
sperm sample
reducing dna
separate sperm
Prior art date
Application number
BR112012025022A
Other languages
English (en)
Inventor
Carmen Lopez Fernandez
Clara Gonzalez Martin
Jaime Gosalvez
Juan Moreno
Michael Evans
Michael Kjelland
Original Assignee
Inguran Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/US2010/054549 external-priority patent/WO2011053727A2/en
Application filed by Inguran Llc filed Critical Inguran Llc
Publication of BR112012025022A2 publication Critical patent/BR112012025022A2/pt

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5091—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing the pathological state of an organism

Abstract

" método de redução da fragmentação de dna em uma amostra de espermatozoides separados" a presente invenção se refere a um método e sistema para o processamento de amostras de células reprodutoras e a separação de espeermatozoides com níveis e ocorrências de fragmentação de dna reduzidos em comparação com os métodos de separação e processamento convencionais, e para o uso de amostras de células reprodutoras e de espermatozoides com baixos níveis de fragmentação de dna para aprimorar a viabilidade de amostras de inseminação, as taxas de fertilidade e de êxito em procedimentos de reprodução assistida, incluindo a inseminação artificial, a fertilização in vitro, a injeção intracitoplasmática, e outras técnicas relacionadas.
BR112012025022A 2010-04-01 2010-12-30 "método de redução da fragmentação de dna em uma amostra de espermatozoides separados" BR112012025022A2 (pt)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US32018310P 2010-04-01 2010-04-01
US32419210P 2010-04-14 2010-04-14
PCT/US2010/054549 WO2011053727A2 (en) 2009-10-30 2010-10-28 Methods and systems for reducing dna fragmentation in a population of sperm cells
PCT/US2010/062598 WO2011123166A2 (en) 2010-04-01 2010-12-30 Methods and systems for reducing dna fragmentation in a processed sperm sample

Publications (1)

Publication Number Publication Date
BR112012025022A2 true BR112012025022A2 (pt) 2015-09-22

Family

ID=44712821

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112012025022A BR112012025022A2 (pt) 2010-04-01 2010-12-30 "método de redução da fragmentação de dna em uma amostra de espermatozoides separados"

Country Status (5)

Country Link
US (1) US20130011825A1 (pt)
CN (1) CN102812123A (pt)
BR (1) BR112012025022A2 (pt)
CA (1) CA2794934A1 (pt)
WO (1) WO2011123166A2 (pt)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10292445B2 (en) * 2011-02-24 2019-05-21 Rochester Institute Of Technology Event monitoring dosimetry apparatuses and methods thereof
DK2761275T3 (en) * 2011-09-30 2017-09-25 Inguran Llc METHODS FOR COLORING AND SORTING SPERMA
BR122016004716A2 (pt) * 2012-10-05 2018-06-19 Inguran, Llc Método de separação de esperma
US10620213B2 (en) 2012-10-05 2020-04-14 Inguran, Llc High pressure sperm sorting and flow cytometer methods
US9189742B2 (en) 2013-11-20 2015-11-17 Justin London Adaptive virtual intelligent agent
CA2905670A1 (en) 2014-09-26 2016-03-26 Inguran, Llc Sex sorted sperm demonstrating a dose response and methods of producing sex sorted sperm demonstrating a dose response
US11279913B2 (en) 2017-01-20 2022-03-22 Inguran, Llc Sperm processing method, apparatus and related media compositions
AR107746A1 (es) * 2017-02-24 2018-05-30 Herberto Ernesto Hector Repetto Dispositivo y método de separación de células móviles
CA3096539A1 (en) * 2018-04-09 2019-10-17 Inguran, Llc Methods and compositions for determining the presence or absence of dna aberrations
CN111323361B (zh) * 2020-03-17 2022-05-10 南通大学 一种快速分离精子头、精子尾和正常有活力精子的方法

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69028526T2 (de) 1989-05-10 1997-02-06 Us Agriculture Verfahren zur vorwahl des geschlechts der nachkommenschaft
US7208265B1 (en) * 1999-11-24 2007-04-24 Xy, Inc. Method of cryopreserving selected sperm cells
US6263745B1 (en) 1999-12-03 2001-07-24 Xy, Inc. Flow cytometer nozzle and flow cytometer sample handling methods
CA2408939C (en) 2000-05-09 2011-11-08 Xy, Inc. High purity x-chromosome bearing and y-chromosome bearing populations of spermatozoa
CN1787739B (zh) * 2002-07-22 2011-03-30 Xy有限责任公司 精子处理系统
DK2309245T3 (en) 2003-03-28 2016-01-04 Inguran Llc Methods for providing sex-sorted animal semen
CN100379429C (zh) * 2003-03-28 2008-04-09 孟山都技术公司 用于精子染色的方法
US7298478B2 (en) 2003-08-14 2007-11-20 Cytonome, Inc. Optical detector for a particle sorting system
US20060270021A1 (en) 2004-06-07 2006-11-30 Shuichi Takayama Integrated microfluidic sperm isolation and insemination device

Also Published As

Publication number Publication date
WO2011123166A2 (en) 2011-10-06
US20130011825A1 (en) 2013-01-10
CN102812123A (zh) 2012-12-05
CA2794934A1 (en) 2011-10-06
WO2011123166A3 (en) 2011-12-29

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Legal Events

Date Code Title Description
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B09B Patent application refused [chapter 9.2 patent gazette]
B09B Patent application refused [chapter 9.2 patent gazette]

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