AR107746A1 - Dispositivo y método de separación de células móviles - Google Patents

Dispositivo y método de separación de células móviles

Info

Publication number
AR107746A1
AR107746A1 ARP170100483A ARP170100483A AR107746A1 AR 107746 A1 AR107746 A1 AR 107746A1 AR P170100483 A ARP170100483 A AR P170100483A AR P170100483 A ARP170100483 A AR P170100483A AR 107746 A1 AR107746 A1 AR 107746A1
Authority
AR
Argentina
Prior art keywords
channel
cell population
chamber
screened plate
mobile cells
Prior art date
Application number
ARP170100483A
Other languages
English (en)
Inventor
Herberto Ernesto Hector Repetto
Original Assignee
Herberto Ernesto Hector Repetto
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Herberto Ernesto Hector Repetto filed Critical Herberto Ernesto Hector Repetto
Priority to ARP170100483A priority Critical patent/AR107746A1/es
Priority to PCT/ES2018/070135 priority patent/WO2018154169A1/es
Priority to CN201880013860.5A priority patent/CN110325632B/zh
Priority to BR112019017410-9A priority patent/BR112019017410A2/pt
Priority to JP2019567400A priority patent/JP7161499B2/ja
Priority to AU2018223254A priority patent/AU2018223254A1/en
Priority to EP18757464.5A priority patent/EP3587561A4/en
Priority to US16/487,995 priority patent/US20200032199A1/en
Priority to CA3053877A priority patent/CA3053877A1/en
Publication of AR107746A1 publication Critical patent/AR107746A1/es
Priority to AU2024201489A priority patent/AU2024201489A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/04—Cell isolation or sorting
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
    • A61D19/00—Instruments or methods for reproduction or fertilisation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D29/00—Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups B01D24/00 - B01D27/00; Filtering elements therefor
    • B01D29/085—Funnel filters; Holders therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/12—Apparatus for enzymology or microbiology with sterilisation, filtration or dialysis means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0608—Germ cells
    • C12N5/0612—Germ cells sorting of gametes, e.g. according to sex or motility
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/34—Purifying; Cleaning

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Molecular Biology (AREA)
  • Veterinary Medicine (AREA)
  • Pathology (AREA)
  • Developmental Biology & Embryology (AREA)
  • General Physics & Mathematics (AREA)
  • Sustainable Development (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Reproductive Health (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

El dispositivo de separación de células móviles a partir de una población celular comprende un primer reservorio y un segundo reservorio vinculados por al menos una placa cribada, caracterizado porque dicha placa cribada comprende al menos un canal con al menos un extremo que presenta una reducción de su diámetro hacia el interior de dicho canal. El método de separación de células móviles a partir de una población celular comprende los siguientes pasos: a) colocar el medio de cultivo para que inunde la cámara 2 y los canales, b) poner en contacto la población celular con una cara de una placa cribada que comprende al menos un canal con al menos un extremo que presenta una reducción de su diámetro hacia el interior de dicho canal, enrasando por debajo del nivel del medio de la cámara 2, lo que permite que siga pasando medio hacia la cámara 1 y así producirse el efecto de contracorriente inicial, c) incubar; d) recuperar las células móviles con mayor porcentaje de morfología normal de la otra cara de dicha placa cribada. El método especialmente se enmarca dentro de las técnicas de recuperación de espermatozoides para una ART (técnica de reproducción asistida) donde se necesita recuperar cantidad y calidad de espermatozoides relacionada a su movilidad, morfología e integridad del ADN sin necesidad de otros dispositivos.
ARP170100483A 2017-02-24 2017-02-24 Dispositivo y método de separación de células móviles AR107746A1 (es)

Priority Applications (10)

Application Number Priority Date Filing Date Title
ARP170100483A AR107746A1 (es) 2017-02-24 2017-02-24 Dispositivo y método de separación de células móviles
PCT/ES2018/070135 WO2018154169A1 (es) 2017-02-24 2018-02-22 Dispositivo y método de separación de células móviles
CN201880013860.5A CN110325632B (zh) 2017-02-24 2018-02-22 用于分离活动细胞的装置和方法
BR112019017410-9A BR112019017410A2 (pt) 2017-02-24 2018-02-22 Dispositivo e método para separar células móveis
JP2019567400A JP7161499B2 (ja) 2017-02-24 2018-02-22 運動細胞を分離する装置および方法
AU2018223254A AU2018223254A1 (en) 2017-02-24 2018-02-22 Device and method for separating mobile cells
EP18757464.5A EP3587561A4 (en) 2017-02-24 2018-02-22 DEVICE AND METHOD FOR SEPARATING MOBILE CELLS
US16/487,995 US20200032199A1 (en) 2017-02-24 2018-02-22 Device and method for separating mobile cells
CA3053877A CA3053877A1 (en) 2017-02-24 2018-02-22 A device and a method to separate motile cells
AU2024201489A AU2024201489A1 (en) 2017-02-24 2024-03-06 A device and a method to separate motile cells

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ARP170100483A AR107746A1 (es) 2017-02-24 2017-02-24 Dispositivo y método de separación de células móviles

Publications (1)

Publication Number Publication Date
AR107746A1 true AR107746A1 (es) 2018-05-30

Family

ID=62597008

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP170100483A AR107746A1 (es) 2017-02-24 2017-02-24 Dispositivo y método de separación de células móviles

Country Status (9)

Country Link
US (1) US20200032199A1 (es)
EP (1) EP3587561A4 (es)
JP (1) JP7161499B2 (es)
CN (1) CN110325632B (es)
AR (1) AR107746A1 (es)
AU (2) AU2018223254A1 (es)
BR (1) BR112019017410A2 (es)
CA (1) CA3053877A1 (es)
WO (1) WO2018154169A1 (es)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AR107746A1 (es) * 2017-02-24 2018-05-30 Herberto Ernesto Hector Repetto Dispositivo y método de separación de células móviles
CN112063502A (zh) * 2020-09-14 2020-12-11 于爱玲 一种基于血液透析器的生物培养装置
CN112662550B (zh) * 2020-10-16 2023-11-10 熹微(苏州)生物医药科技有限公司 一种从精液样本中分离精子的装置和方法
ES2922353A1 (es) 2021-03-03 2022-09-13 Inst Bernabeu S L Placa de seleccion de espermatozoides para microinyeccion intracitoplasmatica
AR125716A1 (es) * 2021-04-21 2023-08-09 Selectivity S A S Un dispositivo de inseminación y un proceso que lo utiliza
WO2023108211A1 (en) * 2021-12-14 2023-06-22 Monash University An apparatus for separating micro-swimmers

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US5575914A (en) 1994-12-23 1996-11-19 Vance Products Incorporated Sperm filter trap having compressed glass wool filter material
US5723319A (en) * 1995-06-05 1998-03-03 Avid Therapeutics, Inc. Cultured cell line that inducibly expresses the hepatitis B virus genome, and uses thereof for screening antiviral substances
US6129214A (en) 1997-12-19 2000-10-10 Bar-Ami; Shalom Sperm strainer system
US6357596B1 (en) 1997-12-19 2002-03-19 Machelle Seibel Sperm strainer system
ATE286677T1 (de) * 2001-06-20 2005-01-15 Fertility Medical Equipment Sc Verfahren und gerät zur herstellung von spermien
US6929945B2 (en) * 2002-12-09 2005-08-16 Advanced Fluidix Laboratories Llc Male fertility assay method and device
WO2004108011A1 (en) * 2003-06-06 2004-12-16 The Regents Of The University Of Michigan Integrated microfluidic sperm isolation and insemination device
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GB2447417A (en) 2007-03-14 2008-09-17 Farhang Abed Apparatus and method for separating motile sperm
EP2414539B1 (en) * 2009-04-03 2020-12-16 The Regents of The University of California Apparatus and method for sorting cells and other biological particulates
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CN102812123A (zh) * 2010-04-01 2012-12-05 英格朗公司 减少经处理的精子样品中的dna断裂的方法和系统
US20120156177A1 (en) * 2010-10-07 2012-06-21 Scarpone Michael A Methods and devices for harvesting and processing connective tissue precursor cells from autologous fat
WO2013040428A1 (en) * 2011-09-14 2013-03-21 Dcb-Usa Llc Microfluidic chips for acquiring sperms with high motility, productions and applications thereof
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ES2725825T3 (es) * 2012-04-16 2019-09-27 Univ Cornell Sistema de fertilización asistida por inyección de esperma intracitoplasmática automatizada
TWI503415B (zh) * 2012-08-10 2015-10-11 Nat Univ Tsing Hua 精蟲篩選系統及其方法
JP6202501B2 (ja) * 2013-03-05 2017-09-27 国立研究開発法人産業技術総合研究所 精子の選抜部構造及び同精子の選抜部構造を備えた精子スクリーニング装置並びに授精用精子液の調製方法
CN105188933B (zh) * 2013-05-03 2017-09-19 莫迪利蒂康特公司 用于分析细胞运动性的设备
CA2834007C (en) 2013-11-19 2022-03-29 The Governing Council Of The University Of Toronto Apparatus and methods for sperm separation
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EP3405560B1 (en) * 2016-01-22 2023-03-29 The Board of Trustees of the Leland Stanford Junior University A micro-fluidic device for selective sorting of highly motile and morphologically normal sperm from unprocessed semen
AR107746A1 (es) * 2017-02-24 2018-05-30 Herberto Ernesto Hector Repetto Dispositivo y método de separación de células móviles
JP6995348B2 (ja) * 2017-11-22 2022-01-14 国立研究開発法人産業技術総合研究所 精子選別方法、精子選別システム、並びに人工授精用精子液として使用する方法
US11491485B2 (en) * 2018-04-09 2022-11-08 Cornell University Rheotaxis-based separation of motile sperm and bacteria using a microfluidic corral system
KR102095267B1 (ko) * 2018-10-22 2020-03-31 한국항공대학교산학협력단 중력 기반 정자 분리 장치 및 분리 방법
GB2583106A (en) * 2019-04-16 2020-10-21 Univ Warwick Motile cell sorting device
WO2021125406A1 (ko) * 2019-12-20 2021-06-24 김영재 정자 추출 마이크로 유체칩 및 그의 정자 추출 방법
CN112662550B (zh) * 2020-10-16 2023-11-10 熹微(苏州)生物医药科技有限公司 一种从精液样本中分离精子的装置和方法
WO2022154755A1 (en) * 2021-01-18 2022-07-21 National University Of Singapore Methods and devices for separation of motile sperm
CN114410428B (zh) * 2022-01-28 2024-03-15 南通大学 一种精子分选的微流控芯片

Also Published As

Publication number Publication date
CN110325632A (zh) 2019-10-11
CA3053877A1 (en) 2018-08-30
JP7161499B2 (ja) 2022-10-26
AU2024201489A1 (en) 2024-03-28
CN110325632B (zh) 2024-04-26
WO2018154169A1 (es) 2018-08-30
EP3587561A1 (en) 2020-01-01
AU2018223254A1 (en) 2019-10-03
JP2020508081A (ja) 2020-03-19
EP3587561A4 (en) 2020-11-11
BR112019017410A2 (pt) 2020-03-31
US20200032199A1 (en) 2020-01-30

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