DE68928914D1 - (in vitro)-vermehrung von embryonalen stammzellen unter verwendung von leukämie-inhibitionsfaktor (lif) - Google Patents

(in vitro)-vermehrung von embryonalen stammzellen unter verwendung von leukämie-inhibitionsfaktor (lif)

Info

Publication number
DE68928914D1
DE68928914D1 DE68928914T DE68928914T DE68928914D1 DE 68928914 D1 DE68928914 D1 DE 68928914D1 DE 68928914 T DE68928914 T DE 68928914T DE 68928914 T DE68928914 T DE 68928914T DE 68928914 D1 DE68928914 D1 DE 68928914D1
Authority
DE
Germany
Prior art keywords
lif
cells
pct
embryonic stem
vitro
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE68928914T
Other languages
English (en)
Other versions
DE68928914T2 (de
Inventor
Robert Williams
Nicholas Gough
Douglas Hilton
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
CSL Innovation Pty Ltd
Original Assignee
Amrad Corp Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amrad Corp Ltd filed Critical Amrad Corp Ltd
Publication of DE68928914D1 publication Critical patent/DE68928914D1/de
Application granted granted Critical
Publication of DE68928914T2 publication Critical patent/DE68928914T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C12N2501/235—Leukemia inhibitory factor [LIF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Environmental Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Reproductive Health (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Animal Behavior & Ethology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Animal Husbandry (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Veterinary Medicine (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
DE68928914T 1988-08-04 1989-08-03 (in vitro)-vermehrung von embryonalen stammzellen unter verwendung von leukämie-inhibitionsfaktor (lif) Expired - Lifetime DE68928914T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
AUPI964488 1988-08-04
PCT/AU1989/000330 WO1990001541A1 (en) 1988-08-04 1989-08-03 In vitro propagation of embryonic stem cells

Publications (2)

Publication Number Publication Date
DE68928914D1 true DE68928914D1 (de) 1999-03-04
DE68928914T2 DE68928914T2 (de) 1999-09-09

Family

ID=3773256

Family Applications (1)

Application Number Title Priority Date Filing Date
DE68928914T Expired - Lifetime DE68928914T2 (de) 1988-08-04 1989-08-03 (in vitro)-vermehrung von embryonalen stammzellen unter verwendung von leukämie-inhibitionsfaktor (lif)

Country Status (11)

Country Link
US (1) US5166065A (de)
EP (1) EP0380646B1 (de)
JP (1) JP2740320B2 (de)
AT (1) ATE175994T1 (de)
AU (1) AU623922B2 (de)
CA (1) CA1341469C (de)
DE (1) DE68928914T2 (de)
DK (1) DK176238B1 (de)
HK (1) HK1003208A1 (de)
NO (1) NO322243B1 (de)
WO (1) WO1990001541A1 (de)

Families Citing this family (99)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030032178A1 (en) * 1988-08-04 2003-02-13 Williams Robert Lindsay In vitro propagation of embryonic stem cells
DE69028514T2 (de) * 1989-01-10 1997-02-20 Amrad Corp Ltd Leukämiehemmender faktor aus vieharten und seine verwendung zur verbesserung der implantation und der entwicklung von embryonalen zellen
AU622515B2 (en) * 1989-01-10 1992-04-09 Amrad Operations Pty. Limited Leukaemia inhibitory factor from livestock species and use thereof to enhance implantation and development of embryonic cells
US5705732A (en) * 1989-06-12 1998-01-06 Oklahoma Medical Research Foundation Universal donor cells
CA2045630A1 (en) * 1989-11-24 1991-05-25 Lawrence Austin Proliferative action of leukaemia inhibitory factor on satellite cells
DE69119759T2 (de) * 1990-03-20 1997-01-16 Amrad Corp Ltd Verfahren zur steuerung der neuronentwicklung und des neuronunterhalts
EP0651787A1 (de) * 1990-07-09 1995-05-10 Amrad Corporation Limited Verbesserte implantation, entwicklung und versorgung von embryonen bei verwendung von leukämieinhibtions-faktor
SG47979A1 (en) * 1991-11-14 1998-04-17 Charles Weissman Transgenic animals lacking prion proteins
US7153684B1 (en) 1992-10-08 2006-12-26 Vanderbilt University Pluripotential embryonic stem cells and methods of making same
US5453357A (en) * 1992-10-08 1995-09-26 Vanderbilt University Pluripotential embryonic stem cells and methods of making same
US5690926A (en) * 1992-10-08 1997-11-25 Vanderbilt University Pluripotential embryonic cells and methods of making same
GB9308271D0 (en) 1993-04-21 1993-06-02 Univ Edinburgh Method of isolating and/or enriching and/or selectively propagating pluripotential animal cells and animals for use in said method
US5523226A (en) * 1993-05-14 1996-06-04 Biotechnology Research And Development Corp. Transgenic swine compositions and methods
AU7677894A (en) * 1993-08-30 1995-03-22 Northwestern University Rat pluripotent embryonic stem cells and method of obtaining and using same
US6686198B1 (en) 1993-10-14 2004-02-03 President And Fellows Of Harvard College Method of inducing and maintaining neuronal cells
ATE355369T1 (de) * 1993-10-14 2006-03-15 Harvard College Verfahren zur induzierung und zur erhaltung neuronalen zellen
US6432711B1 (en) * 1993-11-03 2002-08-13 Diacrin, Inc. Embryonic stem cells capable of differentiating into desired cell lines
GB9401380D0 (en) * 1994-01-25 1994-03-23 Pharmaceutical Proteins Ltd Embryonic cell isolation
FR2726003B1 (fr) * 1994-10-21 2002-10-18 Agronomique Inst Nat Rech Milieu de culture de cellules embryonnaires totipotentes aviaires, procede de culture de ces cellules, et cellules embryonnaires totipotentes aviaires
US5874301A (en) * 1994-11-21 1999-02-23 National Jewish Center For Immunology And Respiratory Medicine Embryonic cell populations and methods to isolate such populations
US5843780A (en) 1995-01-20 1998-12-01 Wisconsin Alumni Research Foundation Primate embryonic stem cells
US5728581A (en) * 1995-06-07 1998-03-17 Systemix, Inc. Method of expanding hematopoietic stem cells, reagents and bioreactors for use therein
EP0882127A4 (de) 1996-01-09 2002-09-11 Univ California Embryonische stammzellen-ähnliche zellen von huftieren, verfahren zur herstellung und verwendung der zellen zur herstellung transgener huftiere
WO1997025413A1 (en) 1996-01-09 1997-07-17 The Regents Of The University Of California Ungulate embryonic stem-like cells, making and using the cells to produce a transgenic ungulate
US5876995A (en) 1996-02-06 1999-03-02 Bryan; Bruce Bioluminescent novelty items
US6247995B1 (en) 1996-02-06 2001-06-19 Bruce Bryan Bioluminescent novelty items
EP0885308A4 (de) * 1996-02-28 2002-02-27 Univ Vanderbilt Verbindungen und methoden zur herstellung embryonaler stammzellen
CA2252524A1 (en) * 1996-04-29 1997-11-06 Leuven Research & Development Vzw Pluripotent rabbit embryonic stem (es) cell lines and their use in the generation of chimeric rabbits
US7544511B2 (en) * 1996-09-25 2009-06-09 Neuralstem Biopharmaceuticals Ltd. Stable neural stem cell line methods
AU3392697A (en) * 1996-06-14 1998-01-07 Regents Of The University Of California, The (in vitro) derivation and culture of primate pluripotent stem cells and therapeutic uses thereof
US6416960B1 (en) 1996-08-08 2002-07-09 Prolume, Ltd. Detection and visualization of neoplastic tissues and other tissues
ATE290205T1 (de) 1996-12-12 2005-03-15 Prolume Ltd Vorrichtung und verfahren zum nachweis und identifizieren von infektiösen wirkstoffen
BR9808192A (pt) * 1997-03-06 2000-10-24 Infigen Inc Processo de clonagem de animais
US6011197A (en) 1997-03-06 2000-01-04 Infigen, Inc. Method of cloning bovines using reprogrammed non-embryonic bovine cells
US6090622A (en) * 1997-03-31 2000-07-18 The Johns Hopkins School Of Medicine Human embryonic pluripotent germ cells
US6331406B1 (en) * 1997-03-31 2001-12-18 The John Hopkins University School Of Medicine Human enbryonic germ cell and methods of use
WO1999020741A1 (en) * 1997-10-23 1999-04-29 Geron Corporation Methods and materials for the growth of primate-derived primordial stem cells
US6027937A (en) * 1998-08-18 2000-02-22 Harper; William Method for propagation of insecta germ cells
US6767737B1 (en) * 1998-08-31 2004-07-27 New York University Stem cells bearing an FGF receptor on the cell surface
US6667176B1 (en) 2000-01-11 2003-12-23 Geron Corporation cDNA libraries reflecting gene expression during growth and differentiation of human pluripotent stem cells
US20020168766A1 (en) * 2000-01-11 2002-11-14 Gold Joseph D. Genetically altered human pluripotent stem cells
US7410798B2 (en) * 2001-01-10 2008-08-12 Geron Corporation Culture system for rapid expansion of human embryonic stem cells
AU759643B2 (en) * 1999-07-20 2003-04-17 University Of Southern California Identification of pluripotent pre-mesenchymal, pre-hematopoietic progenitor cell
US6333192B1 (en) * 1999-08-09 2001-12-25 North Carolina State University Method of producing an undifferentiated avian cell culture using avian primordial germ cells
US7455983B2 (en) * 2000-01-11 2008-11-25 Geron Corporation Medium for growing human embryonic stem cells
US6828145B2 (en) 2000-05-10 2004-12-07 Cedars-Sinai Medical Center Method for the isolation of stem cells by immuno-labeling with HLA/MHC gene product marker
AU2001265268A1 (en) * 2000-06-01 2001-12-11 Pharmacia And Upjohn Company Mice heterozygous for wfs1 gene as mouse models for depression
ES2334639T3 (es) * 2000-06-14 2010-03-15 Vistagen, Inc. Tipificacion de toxicidad empleando celulas madre hepaticas.
US7129084B2 (en) * 2000-08-03 2006-10-31 Therapeutic Human Polyclonals, Inc. Production of humanized antibodies in transgenic animals
EP1217071B1 (de) 2000-12-22 2010-04-28 Institut National De La Recherche Agronomique Positionsunabhängige und gewebespezifische Expression eines Transgens in der Milch eines Transgen-Tieres
JP2005503759A (ja) * 2001-01-24 2005-02-10 アメリカ合衆国 幹細胞の膵臓内分泌細胞への分化方法
US20030211605A1 (en) * 2001-05-01 2003-11-13 Lee Sang-Hun Derivation of midbrain dopaminergic neurons from embryonic stem cells
US7129034B2 (en) * 2001-10-25 2006-10-31 Cedars-Sinai Medical Center Differentiation of whole bone marrow
US6759244B2 (en) 2001-11-08 2004-07-06 Art Institute Of New York And New Jersey, Inc. Composite blastocysts (CBs) from aggregates of dissociated cells of non-viable pre-embryos
AU2002218893A1 (en) * 2001-12-21 2003-07-09 Thromb-X Nv Compositions for the in vitro derivation and culture of embryonic stem (es) cell lines with germline transmission capability
WO2003054170A1 (en) 2001-12-21 2003-07-03 Thromb-X Nv Compositions for the in vitro derivation and culture of embryonic stem (es) cell lines with germline transmission capability and for the culture of adult stem cells
GB0220145D0 (en) * 2002-08-30 2002-10-09 Thromb X Nv Novel compositions for the in vitro derivation and culture of embryonic stem (ES) cell lines with germline transmission capability
US7145057B2 (en) 2002-02-01 2006-12-05 Origen Therapeutics, Inc. Chimeric bird from embryonic stem cells
FR2836924B1 (fr) 2002-03-08 2005-01-14 Vivalis Lignees de cellules aviaires utiles pour la production de substances d'interet
US20030207448A1 (en) * 2002-05-06 2003-11-06 Revera Gregory Henry Methodologies for the creation of pluripotent or multipotent human stem cells without creating or destroying a human embryo
AU2003280687A1 (en) * 2002-10-31 2004-05-25 Hitoshi Niwa Composition for culturing multipotent stem cells and utilization of the same
WO2004053071A2 (en) * 2002-12-09 2004-06-24 Judith Kelleher-Andersson Method for discovering neurogenic agents
US8293488B2 (en) 2002-12-09 2012-10-23 Neuralstem, Inc. Method for screening neurogenic agents
ES2571355T3 (es) * 2002-12-16 2016-05-24 Technion Res & Dev Foundation Sistema de cultivo sin células alimentadoras ni xenocontaminantes para células madre embrionarias humanas
US20040191839A1 (en) * 2003-03-28 2004-09-30 National Institute Of Agrobiological Sciences Japan Methods for sorting undifferentiated cells and uses thereof
IL155783A (en) 2003-05-05 2010-11-30 Technion Res & Dev Foundation Multicellular systems of multi-potential embryonic human stem cells and cancer cells and their use
AU2004320466A1 (en) * 2003-06-11 2006-02-02 Jan Remmereit Nuclear reprogramming of cells for therapeutic use
NZ551832A (en) * 2004-06-09 2010-01-29 Univ Edinburgh Neural stem cells
US20050277124A1 (en) 2004-06-10 2005-12-15 White Steven M Cardiac conduction system cells and uses thereof
US20050282273A1 (en) * 2004-06-10 2005-12-22 Swiatek Donald W Ii Medium and methods for culturing of avian primordial germ cells
US20090124013A1 (en) * 2004-08-16 2009-05-14 Kouns George S Stem-Like Cells
KR101429284B1 (ko) 2004-11-17 2014-08-11 뉴럴스템, 인크. 신경퇴행성 증상의 치료를 위한 인간 신경 세포의 이식
CN1298843C (zh) * 2005-02-07 2007-02-07 十堰市太和医院 不依赖饲养细胞的人胚干细胞培养基
EP3599277A1 (de) 2005-06-22 2020-01-29 Asterias Biotherapeutics, Inc. Suspensionskultur von menschlichen embryonenstammzellen
AU2007257594B2 (en) 2006-03-07 2012-11-01 Geeta Shroff Compositions comprising human embryonic stem cells and their derivatives, methods of use, and methods of preparation
EP2101724B1 (de) 2006-05-11 2020-12-02 Regenics AS Verabreichung von zellextrakten zur verjüngung
US20080003676A1 (en) * 2006-06-26 2008-01-03 Millipore Corporation Growth of embryonic stem cells
AU2007283564B2 (en) 2006-08-09 2013-07-25 Valneva Method of production of transgenic avian using embryonic stem cells
EP2064325B1 (de) 2006-09-01 2011-12-07 Therapeutic Human Polyclonals, Inc. Erhöhte expression von humanem oder humanisiertem immunglobulin bei nicht-humanen transgenen tieren
WO2008066658A2 (en) * 2006-11-03 2008-06-05 The Trustees Of Princeton University Engineered cellular pathways for programmed autoregulation of differentiation
US20080206864A1 (en) * 2006-11-22 2008-08-28 Derrick Rancourt Methods and Materials for Culturing Murine Embryonic Stem Cells
US20080267874A1 (en) * 2007-03-28 2008-10-30 The Buck Institute For Age Research Targeted Neuronal And Glial Human Embryonic Stem Cell Line
EP1985305A1 (de) 2007-04-24 2008-10-29 Vivalis Aus Entenembryonen gewonnene Stammzellkulturen zur Herstellung von Impfstoffen gegen Viren
DK2296676T3 (en) 2008-05-09 2016-12-19 Regenics As cellular extracts
WO2010075500A1 (en) 2008-12-23 2010-07-01 Stemcells California, Inc Target populations of oligodendrocyte precursor cells and methods of making and using same
US8642334B2 (en) 2009-02-17 2014-02-04 Memorial Sloan Kettering Cancer Center Methods of neural conversion of human embryonic stem cells
CN101962630B (zh) * 2009-07-23 2013-04-10 北京大学 诱导人胚胎干细胞或人诱导形成的多潜能干细胞向肝细胞分化的方法
WO2011138687A2 (en) 2010-05-06 2011-11-10 Regenics As Use of cellular extracts for skin rejuvenation
KR101765980B1 (ko) 2010-07-28 2017-08-07 뉴럴스템, 인크. 신경퇴행성 질환 및/또는 장애의 치료 및/또는 역전 방법
US10280398B2 (en) 2011-11-04 2019-05-07 Memorial Sloan-Kettering Cancer Center Midbrain dopamine (DA) neurons for engraftment
AU2013356930B2 (en) 2012-12-10 2017-01-05 Regenics As Use of egg cellular extracts for skin rejuvenation
AU2014256876B2 (en) 2013-04-26 2020-11-12 Cornell, University Cortical interneurons and other neuronal cells produced by the directed differentiation of pluripotent and multipotent cells
KR20230008892A (ko) 2013-11-21 2023-01-16 메모리얼 슬로안 케터링 캔서 센터 인간 다능성 줄기 세포로부터 기능적 두개 기원판 유도체의 전문화
US20180363070A9 (en) 2014-08-12 2018-12-20 Univ Wayne State Systems and methods to detect stem cell stress and uses thereof
MX2017005186A (es) 2014-10-20 2017-07-26 Neuralstem Inc Celulas madre neurales estables que comprenden un polinucleótido exógeno que codifica un factor de crecimiento y métodos de uso de las mismas.
RU2741786C2 (ru) 2016-05-25 2021-01-28 Энсэрм (Энститю Насьональ Де Ла Санте Э Де Ла Решерш Медикаль) Способы и композиции для лечения опухолей
US11679148B2 (en) 2017-11-24 2023-06-20 Institut National De La Santé Et De La Recherche Médicale (Inserm) Methods and compositions for treating cancers
CN112703011A (zh) 2018-08-06 2021-04-23 国家医疗保健研究所 用于治疗癌症的方法和组合物
EP4314246A1 (de) 2021-04-01 2024-02-07 INSERM (Institut National de la Santé et de la Recherche Médicale) Verfahren zur herstellung von leberorganoiden, damit erhaltene leberorganoide und verwendungen davon

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PT87133B (pt) * 1987-04-02 1992-07-31 Amrad Corp Ltd Metodo de purificacao do factor inibidor da leucemia (lif) e de composicoes farmaceuticas contendo polipeptidos com actividade do lif

Also Published As

Publication number Publication date
WO1990001541A1 (en) 1990-02-22
HK1003208A1 (en) 1998-10-16
EP0380646B1 (de) 1999-01-20
CA1341469C (en) 2004-12-28
AU623922B2 (en) 1992-05-28
EP0380646A4 (en) 1991-01-23
JPH03503241A (ja) 1991-07-25
DK176238B1 (da) 2007-04-10
JP2740320B2 (ja) 1998-04-15
ATE175994T1 (de) 1999-02-15
NO910385D0 (no) 1991-02-01
DK17091A (da) 1991-04-04
NO322243B1 (no) 2006-09-04
AU4059089A (en) 1990-03-05
NO910385L (no) 1991-04-04
EP0380646A1 (de) 1990-08-08
DE68928914T2 (de) 1999-09-09
DK17091D0 (da) 1991-01-31
US5166065A (en) 1992-11-24

Similar Documents

Publication Publication Date Title
DE68928914D1 (de) (in vitro)-vermehrung von embryonalen stammzellen unter verwendung von leukämie-inhibitionsfaktor (lif)
EP0453453A4 (en) Leukaemia inhibitory factor from livestock species and use thereof to enhance implantation and development of embryonic cells
EG17665A (en) Substrat pour culture hors-soil
DE69735293D1 (de) Kultur einer zelllinieaus der inneren zellmasse von huftierembryonen.
DK1044262T3 (da) Könsspecifik inseminering af pattedyr med et lavt antal sædceller
DK335388D0 (da) Fremgangsmaade til dyrkning af embryoner
AU1595195A (en) Embryonic stem cells as nuclear donors and nuclear transfer techniques to produce chimeric and transgenic animals
ATE82588T1 (de) Zuchtverfahren fuer leukocyten.
DK0477290T3 (da) Fremgangsmåder, sammensætninger og indretninger til dyrkning af celler
DE69535263D1 (de) Angiotensin ii zur verbesserung der befruchtung
NO912621L (no) Fremgangsmaate for innfoering av dna i animalske celler.
BR9908744A (pt) Uso de um meio de cultura
DE69002770D1 (de) Fluessiges enzymatisches reinigungsmittel.
DE69117846D1 (de) Einstufiges Kulturverfahren für Vogelembryos
ATE176846T1 (de) Verfahren zum zuchten von hummelköniginnen und verfahren zum zuchten von hummeln
ATE106899T1 (de) Inhibitor der proliferation von endothelzellen.
Uchida et al. Proliferation of mouse primordial germ cells on the bone marrow-derived stromal cell line ST2
DK197091A (da) Fremgangsmaade til fremstilling af proteiner med fviii aktivitet og/eller fviii derivater
MX9603037A (es) Materiales y metodos para manejar rechazo hiperagudo en xenotrasplante humano.
ATE36962T1 (de) Nichtchirurgisches geraet zur transplantation eines menschlichen embryos.
ES534347A0 (es) Mejoras introducidas en el objeto de la patente principal por: procedimiento de obtencion de sales de trialquisulfonio de n-fosfonometilglicina reguladoras del crecimiento vegetal.

Legal Events

Date Code Title Description
8364 No opposition during term of opposition