DE68906799D1 - Verfahren zur reinigung von nukleinsaeuren. - Google Patents

Verfahren zur reinigung von nukleinsaeuren.

Info

Publication number
DE68906799D1
DE68906799D1 DE8989903325T DE68906799T DE68906799D1 DE 68906799 D1 DE68906799 D1 DE 68906799D1 DE 8989903325 T DE8989903325 T DE 8989903325T DE 68906799 T DE68906799 T DE 68906799T DE 68906799 D1 DE68906799 D1 DE 68906799D1
Authority
DE
Germany
Prior art keywords
nucleic acids
enzymes
purifying nucleic
silica gel
contg
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE8989903325T
Other languages
English (en)
Other versions
DE68906799T2 (de
Inventor
Cormick Randy Miles Mc
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
EIDP Inc
Original Assignee
EI Du Pont de Nemours and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by EI Du Pont de Nemours and Co filed Critical EI Du Pont de Nemours and Co
Publication of DE68906799D1 publication Critical patent/DE68906799D1/de
Application granted granted Critical
Publication of DE68906799T2 publication Critical patent/DE68906799T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/82—Proteins from microorganisms
    • Y10S530/821—Separation of nucleic acid

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Saccharide Compounds (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Detergent Compositions (AREA)
  • Silicon Compounds (AREA)
DE8989903325T 1987-12-28 1989-03-10 Verfahren zur reinigung von nukleinsaeuren. Expired - Fee Related DE68906799T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US07/138,038 US4923978A (en) 1987-12-28 1987-12-28 Process for purifying nucleic acids
PCT/US1989/000902 WO1990010637A1 (en) 1987-12-28 1989-03-10 Method for purifying nucleic acids

Publications (2)

Publication Number Publication Date
DE68906799D1 true DE68906799D1 (de) 1993-07-01
DE68906799T2 DE68906799T2 (de) 1993-09-02

Family

ID=42211805

Family Applications (1)

Application Number Title Priority Date Filing Date
DE8989903325T Expired - Fee Related DE68906799T2 (de) 1987-12-28 1989-03-10 Verfahren zur reinigung von nukleinsaeuren.

Country Status (10)

Country Link
US (1) US4923978A (de)
EP (1) EP0462100B1 (de)
JP (1) JP2703636B2 (de)
AT (1) ATE89827T1 (de)
AU (2) AU625639B2 (de)
DE (1) DE68906799T2 (de)
DK (1) DK175017B1 (de)
FI (1) FI95270C (de)
NO (1) NO177856C (de)
WO (1) WO1990010637A1 (de)

Families Citing this family (85)

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US6093558A (en) * 1991-07-25 2000-07-25 Edge Biosystems, Inc. Binding protein of biologically active compositions to an adhesive formulation on a substrate
US5329000A (en) * 1991-10-31 1994-07-12 Becton, Dickinson And Company Purification of DNA with silicon tetrahydrazide
DE4139664A1 (de) * 1991-12-02 1993-06-03 Diagen Inst Molekularbio Vorrichtung und verfahren zur isolierung und reinigung von nukleinsaeuren
US5438128A (en) * 1992-02-07 1995-08-01 Millipore Corporation Method for rapid purifiction of nucleic acids using layered ion-exchange membranes
DE69324716T2 (de) * 1992-02-13 1999-09-09 Becton Dickinson Co Celithydrat und Reinigung von DNS
CA2136432A1 (en) * 1992-06-23 1994-01-06 Rex M. Bitner Deproteinization with azlactone-functional supports
EP0580305B1 (de) * 1992-07-02 1999-09-22 Edge Biosystems, Inc. Verfahren und Material für Reinigung von Nukleinsäuren
CA2102264C (en) * 1992-11-13 2000-08-01 Daniel Lee Woodard Boron silicates, aluminum silicates, phosphosilicates and purification of dna
ES2171450T3 (es) * 1993-05-24 2002-09-16 Np Predpr Farmek Adn de bajo peso molecular procedente de lecha de esturion, procedimiento para obtener el adn y preparacion farmaceutica basada en el mismo.
US5561064A (en) * 1994-02-01 1996-10-01 Vical Incorporated Production of pharmaceutical-grade plasmid DNA
DE4432654C2 (de) 1994-09-14 1998-03-26 Qiagen Gmbh Verfahren zur Isolierung von Nukleinsäuren aus natürlichen Quellen
GB9425138D0 (en) 1994-12-12 1995-02-08 Dynal As Isolation of nucleic acid
US5804684A (en) * 1995-08-24 1998-09-08 The Theobald Smith Research Institute, Inc. Method for isolating nucleic acids
US5783686A (en) * 1995-09-15 1998-07-21 Beckman Instruments, Inc. Method for purifying nucleic acids from heterogenous mixtures
AU5512598A (en) * 1996-11-27 1998-06-22 Edge Biosystems, Inc. Matrix for rapid purification of nucleic acids
EP0991456B1 (de) * 1997-06-27 2003-09-03 Life Technologies, Inc. Einstufige vorrichtung und verfahren zur konzentration und säuberung biologischer moleküle
US5958677A (en) * 1997-07-28 1999-09-28 The New York Blood Center, Inc. Method for purifying viral nucleic acids
US6368871B1 (en) * 1997-08-13 2002-04-09 Cepheid Non-planar microstructures for manipulation of fluid samples
KR20010032806A (ko) * 1997-12-06 2001-04-25 매튜 존 베이커 핵산 분리방법
US6914137B2 (en) * 1997-12-06 2005-07-05 Dna Research Innovations Limited Isolation of nucleic acids
ATE400358T1 (de) 1997-12-24 2008-07-15 Cepheid Vorrichtung und verfahren zur lyse
CA2320470A1 (en) * 1998-02-05 1999-08-12 Henry M. Jackson Foundation For The Advancement Of Military Medicine Simplified method for removing free protein during preparation of protein-polysaccharide conjugates and vaccines using restricted-access media
US5973138A (en) * 1998-10-30 1999-10-26 Becton Dickinson And Company Method for purification and manipulation of nucleic acids using paramagnetic particles
US6431476B1 (en) 1999-12-21 2002-08-13 Cepheid Apparatus and method for rapid ultrasonic disruption of cells or viruses
US7914994B2 (en) 1998-12-24 2011-03-29 Cepheid Method for separating an analyte from a sample
US20040200909A1 (en) * 1999-05-28 2004-10-14 Cepheid Apparatus and method for cell disruption
CA2374423C (en) * 1999-05-28 2013-04-09 Cepheid Apparatus and method for analyzing a liquid sample
US9073053B2 (en) 1999-05-28 2015-07-07 Cepheid Apparatus and method for cell disruption
US8815521B2 (en) 2000-05-30 2014-08-26 Cepheid Apparatus and method for cell disruption
US6878540B2 (en) * 1999-06-25 2005-04-12 Cepheid Device for lysing cells, spores, or microorganisms
US6479296B1 (en) * 1999-07-07 2002-11-12 Alex G. Bonner Solid phase precipitation and extraction
US6383783B1 (en) 1999-09-21 2002-05-07 3M Innovative Properties Company Nucleic acid isolation by adhering to hydrophobic solid phase and removing with nonionic surfactant
US20020012990A1 (en) * 1999-12-22 2002-01-31 Lander Russel Jackson Process for the scaleable purification of plasmid DNA
AU2001273432A1 (en) * 2000-07-13 2002-01-30 Invitrogen Corporation Methods and compositions for rapid protein and peptide extraction and isolation using a lysis matrix
WO2002008237A2 (en) * 2000-07-21 2002-01-31 Lyles Mark B Materials and methods for binding nucleic acids to surfaces
US7208271B2 (en) 2001-11-28 2007-04-24 Applera Corporation Compositions and methods of selective nucleic acid isolation
US7347976B2 (en) 2001-12-20 2008-03-25 3M Innovative Properties Company Methods and devices for removal of organic molecules from biological mixtures using a hydrophilic solid support in a hydrophobic matrix
US7192560B2 (en) * 2001-12-20 2007-03-20 3M Innovative Properties Company Methods and devices for removal of organic molecules from biological mixtures using anion exchange
CN1617938A (zh) 2002-01-16 2005-05-18 戴诺生物技术有限公司 从单个样品中分离核酸和蛋白质的方法
WO2003070898A2 (en) * 2002-02-19 2003-08-28 Choicepoint Asset Company Selective extraction of dna from groups of cells
GB0212826D0 (en) * 2002-05-31 2002-07-10 Dna Res Innovations Ltd Materials and methods relating to polyions and substance delivery
US7879621B2 (en) * 2003-05-08 2011-02-01 Phynexus, Inc. Open channel solid phase extraction systems and methods
AU2003293042A1 (en) * 2002-12-23 2004-07-29 Vical Incorporated Process for purification of plasmid dna
US7601491B2 (en) 2003-02-06 2009-10-13 Becton, Dickinson And Company Pretreatment method for extraction of nucleic acid from biological samples and kits therefor
US20040157219A1 (en) * 2003-02-06 2004-08-12 Jianrong Lou Chemical treatment of biological samples for nucleic acid extraction and kits therefor
US7981600B2 (en) * 2003-04-17 2011-07-19 3M Innovative Properties Company Methods and devices for removal of organic molecules from biological mixtures using an anion exchange material that includes a polyoxyalkylene
GB0317199D0 (en) 2003-07-23 2003-08-27 Cyclops Genome Sciences Ltd Clean-up beads
WO2005012522A1 (en) * 2003-07-23 2005-02-10 Cyclops Genome Sciences Limited Clean-up beads
EP2311994A1 (de) * 2003-08-01 2011-04-20 Life Technologies Corporation Zusammensetzungen und Verfahren zur Aufreinigung kurzer RNA-Moleküle und anderer Nukleinsäuren
ATE466105T1 (de) * 2003-09-12 2010-05-15 Biocontrol Systems Inc Verfahren, zusammensetzungen und kits zur anreicherung und zum nachweis von mikroorganismen
US20050130177A1 (en) * 2003-12-12 2005-06-16 3M Innovative Properties Company Variable valve apparatus and methods
US7727710B2 (en) * 2003-12-24 2010-06-01 3M Innovative Properties Company Materials, methods, and kits for reducing nonspecific binding of molecules to a surface
US7939249B2 (en) * 2003-12-24 2011-05-10 3M Innovative Properties Company Methods for nucleic acid isolation and kits using a microfluidic device and concentration step
US20050239091A1 (en) * 2004-04-23 2005-10-27 Collis Matthew P Extraction of nucleic acids using small diameter magnetically-responsive particles
CA2575784A1 (en) * 2004-08-03 2006-02-16 Becton, Dickinson And Company Use of magnetic material to fractionate samples
AU2005271688B2 (en) * 2004-08-03 2011-10-06 Becton, Dickinson And Company Use of magnetic material to direct isolation of compounds and fractionation of multipart samples
EP1856262B1 (de) 2005-01-31 2012-08-15 Merck Sharp & Dohme Corp. Upstream- und downstream-reinigungsverfahren zur massenfertigung von plasmider dna
US20090215124A1 (en) 2005-02-15 2009-08-27 Weidong Cao Nucleic acid isolation methods and materials and devices thereof
US20060202922A1 (en) * 2005-03-10 2006-09-14 Hanna Christopher P Method for optimizing a purification procedure
US7378260B2 (en) * 2005-04-01 2008-05-27 Applera Corporation Products and methods for reducing dye artifacts
WO2008134470A2 (en) * 2007-04-25 2008-11-06 3M Innovative Properties Company Methods for nucleic acid amplification
EP2171098B1 (de) * 2007-06-29 2018-03-28 Becton, Dickinson and Company Verfahren zur extraktion und aufreinigung von bestandteilen biologischer proben
US20090088560A1 (en) * 2007-10-02 2009-04-02 Hong Shen Process for Nucleic Acid Purification
US7759112B2 (en) 2007-10-31 2010-07-20 Akonni Biosystems, Inc. Apparatus, system, and method for purifying nucleic acids
ES2588908T3 (es) 2007-11-21 2016-11-07 Cosmosid Inc. Sistema de identificación de genoma
US8478544B2 (en) 2007-11-21 2013-07-02 Cosmosid Inc. Direct identification and measurement of relative populations of microorganisms with direct DNA sequencing and probabilistic methods
DE102008010693A1 (de) 2008-02-22 2009-08-27 Qiagen Gmbh Neue Matrixmaterialien, Verfahren zu ihrer Herstellung und ihre Verwendung in Verfahren zur Isolierung von Biomolekülen
EP2593770B1 (de) * 2010-07-14 2020-05-06 Qiagen GmbH Vorrichtung zur isolierung und/oder reinigung von biomolekülen
PT3270141T (pt) 2011-03-08 2020-08-28 Univ Laval Dispositivo centrípeto fluídico
KR101365737B1 (ko) 2012-08-28 2014-02-20 한정헌 생물학적 시료로부터 핵산 증폭 반응용 생물학적 분자를 신속하게 분리하기 위한 다공성 고체상 및 이의 용도
PL2812091T3 (pl) 2012-09-17 2021-07-19 W.R. Grace & Co. - Conn. Podłoża chromatograficzne i urządzenia
GB201217405D0 (en) 2012-09-28 2012-11-14 Fermentas Uab Protein removal agent
EP3049539B1 (de) 2013-09-25 2018-09-05 Bio-Id Diagnostic Inc. Verfahren für den nachweis von nukleinsäurefragmenten
PL3137209T3 (pl) 2014-05-02 2023-01-02 W.R. Grace & Co. - Conn. Funkcjonalizowany materiał nośnikowy i sposoby wytwarzania oraz stosowania funkcjonalizowanego materiału nośnikowego
JP2018517559A (ja) 2015-06-05 2018-07-05 ダブリュー・アール・グレース・アンド・カンパニー−コーンＷ Ｒ Ｇｒａｃｅ ＆ Ｃｏ−Ｃｏｎｎ 吸着性バイオプロセス清澄化剤並びにその製造及び使用方法
USD799715S1 (en) 2015-10-23 2017-10-10 Gene POC, Inc. Fluidic centripetal device
CN117448315A (zh) 2017-09-18 2024-01-26 相达生物科技国际有限公司 使用双水相系统来分离、纯化和/或浓缩短链核酸片段的方法
KR20220075978A (ko) 2020-11-30 2022-06-08 (주)바이오니아 중저 비유전율을 가진 화합물을 포함한 핵산 결합 완충액을 이용한 핵산 분리방법

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3639949A1 (de) * 1986-11-22 1988-06-09 Diagen Inst Molekularbio Verfahren zur trennung von langkettigen nukleinsaeuren

Also Published As

Publication number Publication date
DE68906799T2 (de) 1993-09-02
WO1990010637A1 (en) 1990-09-20
EP0462100B1 (de) 1993-05-26
NO177856B (no) 1995-08-28
ATE89827T1 (de) 1993-06-15
NO177856C (no) 1995-12-06
JPH04503942A (ja) 1992-07-16
FI914240A0 (fi) 1991-09-09
AU625639B2 (en) 1992-07-16
DK156191D0 (da) 1991-09-05
AU3352589A (en) 1990-10-09
NO913545L (no) 1991-09-09
NO913545D0 (no) 1991-09-09
AU632284B2 (en) 1992-12-24
JP2703636B2 (ja) 1998-01-26
DK175017B1 (da) 2004-04-26
DK156191A (da) 1991-09-05
FI95270B (fi) 1995-09-29
FI95270C (fi) 1996-01-10
EP0462100A1 (de) 1991-12-27
US4923978A (en) 1990-05-08

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee